BBa_K394004 1 BBa_K394004 An GAL1 Inducible Sex-Lethal Construct with a His tag 2010-10-19T11:00:00Z 2015-05-08T01:12:21Z none This construct was used to selectively activate Sex-Lethal in a yeast cell false false _504_ 0 6327 9 Not in stock true none false Brian Landry component2091004 1 BBa_J63003 component2091005 1 BBa_J63007 component2091002 1 BBa_J63006 component2091006 1 BBa_K394003 component2091008 1 BBa_J63002 annotation2091002 1 BBa_J63006 range2091002 1 1 549 annotation2091005 1 BBa_J63007 range2091005 1 584 613 annotation2091008 1 BBa_J63002 range2091008 1 1690 1914 annotation2091006 1 BBa_K394003 range2091006 1 620 1681 annotation2091004 1 BBa_J63003 range2091004 1 558 575 BBa_J63003 1 Kozak & st designed yeast Kozak sequence 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z consensus Kozak and start built from oligonucleotides Released HQ 2013 consensus Kozak sequence and start codon false false _97_ 0 545 97 In stock false designed such that fusion using fusion bricks assembly method leads to in frame translation true Caroline Ajo-Franklin annotation1902852 1 start codon range1902852 1 13 15 BBa_J63007 1 PKI NES PKI nuclear export sequence; yeast codon optimized 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z from genomic DNA yeast codon optimized PKI nuclear export sequence false false _97_ 0 545 97 It's complicated false optimized codons for S. cerevisiae false Caroline Ajo-Franklin BBa_J63002 1 tADH1 ADH1 terminator from S. cerevisiae 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z genomic DNA of S. cerevisiae ADH1 terminator from S. cerevisiae false true _97_ 0 545 97 It's complicated false starts with stop codon false Caroline Ajo-Franklin annotation1902851 1 ADH1 terminator range1902851 1 1 225 BBa_J63006 1 GAL1 yeast GAL1 promoter 2006-10-10T11:00:00Z 2015-08-31T01:56:26Z genomic GAL1 locus Released HQ 2013 GAL1 promoter from S. cerevisiae false true _97_ 0 545 97 In stock false unchanged from genomic sequence true Caroline Ajo-Franklin BBa_K394003 1 BBa_K394003 Sex-Lethal from Drosophila melanogaster 2010-10-19T11:00:00Z 2015-05-08T01:12:21Z This was part was made using PCR from a construct synthesized by Mr. Gene. It intons have been artificially removed. This is the coding region for the Sex-Lethal Gene from which can be used to control splicing events. false false _504_ 0 6327 9 Not in stock false none false Brian Landry annotation2102751 1 Sxl gene range2102751 1 1 1062 annotation2095519 1 Start Codon range2095519 1 1 3 BBa_J63006_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggag BBa_J63002_sequence 1 taataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_K394004_sequence 1 ccccattatcttagcctaaaaaaaccttctctttggaactttcagtaatacgcttaactgctcattgctatattgaagtacggattagaagccgccgagcgggtgacagccctccgaaggaagactctcctccgtgcgtcctcgtcttcaccggtcgcgttcctgaaacgcagatgtgcctcgcgccgcactgctccgaacaataaagattctacaatactagcttttatggttatgaagaggaaaaattggcagtaacctggccccacaaaccttcaaatgaacgaatcaaattaacaaccataggatgataatgcgattagttttttagccttatttctggggtaattaatcagcgaagcgatgatttttgatctattaacagatatataaatgcaaaaactgcataaccactttaactaatactttcaacattttcggtttgtattacttcttattcaaatgtaataaaagtatcaacaaaaaattgttaatatacctctatactttaacgtcaaggaggaaactagacccgccgccaccatggagtactagagcccgccgccaccatggagtactagagttggctttgaaattggctggtttggatatttactagatgtacggcaacaataatccgggtagtaacaataataatggtggttatcccccatatggctacaacaacaagtcgagtggtgggcgtggatttgggatgtcccattctctgccatccggaatgtcacgttatgcgttctcgccacaggatacagaattttcatttccaagttcatcgtcgcgtcgtggttacaatgatttccccggctgcggcggcagcggcggaaatggtgggagtgccaacaatttagggggcggcaacatgtgccacctgccgccgatggccagcaacaactcgctgaataatctctgcggattgtcgctgggcagcggtggtagtgatgatctcatgaacgatcctcgggcaagcaacaccaacctgattgtcaactacttgccccaggacatgaccgatcgcgagctgtacgccctattcagagccattggacccatcaacacgtgcagaatcatgcgagactataagactggctacagttttggttatgctttcgtggacttcacatcggaaatggactcgcagcgtgctattaaagtgctgaatggcatcacagtgcgcaacaagcggcttaaggtttcctatgcacgtcccggcggagaatcgatcaaggacaccaatctgtatgtgaccaatctgccgcgtaccataaccgacgatcagctggacacgatcttcggcaagtacggttccattgtgcagaagaacatcttgcgtgacaagctcacaggtcgtcctcgtggtgtggcctttgttcggtacaacaagcgtgaggaggcccaggaggccatttcggcgctgaacaacgtaatacccgagggcggatcacagccgctgtccgtccggttggctgaggagcatggcaaggcgaaggcggcccactttatgtcgcagatgggcgtggttccagcaaatgtaccaccgccgccaccacaaccgccagcacatatggccgccgcattcaacatgatgcacagaggtagatcaattaaatcacagcaacgcttccaaaactcgcatccctatttcgatgctaaaaagtttatctactagagtaataagcgaatttcttatgatttatgatttttattattaaataagttataaaaaaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattcttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctcttattgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccc BBa_K394003_sequence 1 atgtacggcaacaataatccgggtagtaacaataataatggtggttatcccccatatggctacaacaacaagtcgagtggtgggcgtggatttgggatgtcccattctctgccatccggaatgtcacgttatgcgttctcgccacaggatacagaattttcatttccaagttcatcgtcgcgtcgtggttacaatgatttccccggctgcggcggcagcggcggaaatggtgggagtgccaacaatttagggggcggcaacatgtgccacctgccgccgatggccagcaacaactcgctgaataatctctgcggattgtcgctgggcagcggtggtagtgatgatctcatgaacgatcctcgggcaagcaacaccaacctgattgtcaactacttgccccaggacatgaccgatcgcgagctgtacgccctattcagagccattggacccatcaacacgtgcagaatcatgcgagactataagactggctacagttttggttatgctttcgtggacttcacatcggaaatggactcgcagcgtgctattaaagtgctgaatggcatcacagtgcgcaacaagcggcttaaggtttcctatgcacgtcccggcggagaatcgatcaaggacaccaatctgtatgtgaccaatctgccgcgtaccataaccgacgatcagctggacacgatcttcggcaagtacggttccattgtgcagaagaacatcttgcgtgacaagctcacaggtcgtcctcgtggtgtggcctttgttcggtacaacaagcgtgaggaggcccaggaggccatttcggcgctgaacaacgtaatacccgagggcggatcacagccgctgtccgtccggttggctgaggagcatggcaaggcgaaggcggcccactttatgtcgcagatgggcgtggttccagcaaatgtaccaccgccgccaccacaaccgccagcacatatggccgccgcattcaacatgatgcacagaggtagatcaattaaatcacagcaacgcttccaaaactcgcatccctatttcgatgctaaaaagtttatc BBa_J63003_sequence 1 cccgccgccaccatggag BBa_J63007_sequence 1 ttggctttgaaattggctggtttggatatt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z