LOCUS       BBa_K395106              969 bp    DNA     linear       23-Dec-2024
DEFINITION  GFP reporter repressed by LuxR and 3OC6HSL (K395009:K121013)
ACCESSION   BBa_K395106
VERSION     BBa_K395106.1  
FEATURES             Location/Qualifiers
     promoter        1..30
                     /label=BBa_K395009
     misc_feature    39..58
                     /label=BBa_J54033
     misc_feature    39..44
                     /label=SalI
     misc_feature    53..58
                     /label=BamHI
     misc_feature    65..84
                     /label=BBa_J54025
     misc_feature    65..70
                     /label=BglII
     misc_feature    79..84
                     /label=MulI
     RBS             87..102
                     /label=RBS-1\Strong
     misc_feature    87..102
                     /label=BBa_B0030
     RBS             94..100
                     /label=RBS
     misc_feature    109..828
                     /label=BBa_E0040
     misc_feature    838..917
                     /label=BBa_B0010
     misc_feature    925..965
                     /label=BBa_B0012
     stem_loop       849..892
                     /label=stem_loop
     stem_loop       937..956
                     /label=T7 TE
     misc_feature    39..969
                     /label=BBa_J54103
     misc_feature    39..43
                     /label=SalI
     misc_feature    39..57
                     /label=BBa_J54033
     misc_feature    53..57
                     /label=BamHI
     misc_feature    66..70
                     /label=BglII
     misc_feature    66..84
                     /label=BBa_J54025
     misc_feature    79..84
                     /label=MulI
     RBS             93..107
                     /label=RBS-1\Strong
     misc_feature    93..107
                     /label=BBa_B0030
     RBS             100..104
                     /label=RBS
     misc_feature    114..833
                     /label=BBa_E0040
     misc_feature    842..921
                     /label=BBa_B0010
     stem_loop       853..896
                     /label=stem_loop
     misc_feature    930..970
                     /label=BBa_B0012
     stem_loop       937..956
                     /label=T7 TE
     polyA_site      957..970
                     /label=polya
     misc_feature    963..963
                     /label=stop
     misc_feature    39..969
                     /label=BBa_J54103
     misc_feature    39..969
                     /label=BBa_K121013
ORIGIN
        1 tttatacctg taggatcgta caggtacaat tactagaggt cgactgacga ctggatccta
       61 tcaagatctg tcggataacg cgttactaga gattaaagag gagaaatact agatgcgtaa
      121 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      181 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
      241 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
      301 tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt
      361 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
      421 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
      481 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
      541 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
      601 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
      661 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
      721 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
      781 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag
      841 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      901 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      961 gcgtttata
//
LOCUS       BBa_K395103              969 bp    DNA     linear       23-Dec-2024
DEFINITION  GFP reporter repressed by LuxR and 3OC6HSL (K395006:K121013)
ACCESSION   BBa_K395103
VERSION     BBa_K395103.1  
FEATURES             Location/Qualifiers
     promoter        1..30
                     /label=BBa_K395006
     misc_feature    39..58
                     /label=BBa_J54033
     misc_feature    39..44
                     /label=SalI
     misc_feature    53..58
                     /label=BamHI
     misc_feature    65..84
                     /label=BBa_J54025
     misc_feature    65..70
                     /label=BglII
     misc_feature    79..84
                     /label=MulI
     RBS             87..102
                     /label=RBS-1\Strong
     misc_feature    87..102
                     /label=BBa_B0030
     RBS             94..100
                     /label=RBS
     misc_feature    109..828
                     /label=BBa_E0040
     misc_feature    838..917
                     /label=BBa_B0010
     misc_feature    925..965
                     /label=BBa_B0012
     stem_loop       849..892
                     /label=stem_loop
     stem_loop       937..956
                     /label=T7 TE
     misc_feature    39..969
                     /label=BBa_J54103
ORIGIN
        1 tttatacctg taggatcgta caggtacaat tactagaggt cgactgacga ctggatccta
       61 tcaagatctg tcggataacg cgttactaga gattaaagag gagaaatact agatgcgtaa
      121 aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg gtgatgttaa
      181 tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg gaaaacttac
      241 ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac ttgtcactac
      301 tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac agcatgactt
      361 tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt tcaaagatga
      421 cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg ttaatagaat
      481 cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca aattggaata
      541 caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg gaatcaaagt
      601 taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag accattatca
      661 acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt acctgtccac
      721 acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc ttcttgagtt
      781 tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat aatactagag
      841 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      901 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      961 gcgtttata
//