BBa_R0061 1 luxR Promoter (HSL-mediated luxR repressor) 2004-01-28T12:00:00Z 2015-05-08T01:14:15Z Released HQ 2013 This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site. false true _1_ 0 24 7 In stock false true Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu annotation305863 1 -35 range305863 1 1 6 annotation305864 1 -10 range305864 1 25 30 annotation305877 1 Lux Box range305877 1 6 25 BBa_J54103 1 BBa_J54103 Promoterless-GFP(A) 2006-10-16T11:00:00Z 2015-08-31T03:48:11Z synthesis Released HQ 2013 promoterless false true _76_ 0 762 76 In stock true as negative control true Yusaku Nakashima annotation2152491 1 BamHI range2152491 1 15 20 annotation2152501 1 stem_loop range2152501 1 811 854 annotation2152498 1 BBa_E0040 range2152498 1 71 790 annotation2152496 1 BBa_B0030 range2152496 1 49 64 annotation2152489 1 BBa_J54033 range2152489 1 1 20 annotation2152500 1 BBa_B0012 range2152500 1 887 927 annotation2152494 1 MulI range2152494 1 41 46 annotation2152499 1 BBa_B0010 range2152499 1 800 879 annotation2152497 1 RBS range2152497 1 56 62 annotation2152495 1 RBS-1\Strong range2152495 1 49 64 annotation2152492 1 BBa_J54025 range2152492 1 27 46 annotation2152502 1 T7 TE range2152502 1 899 918 annotation2152493 1 BglII range2152493 1 27 32 annotation2152490 1 SalI range2152490 1 1 6 BBa_K395104 1 BBa_K395104 GFP reporter repressed by LuxR and 3OC6HSL (K395007:K121013) 2010-10-04T11:00:00Z 2015-05-08T01:12:21Z We ligated BBa_K395007 into BBa_K121013. This part is GFP reporter which is represed by LuxR and 3OC6HSL, and the promoter is K395007. And we use this part to measure the activity of BBa_K395007. true false _505_ 0 6370 9 Discontinued false yes false Shohei Kitano component2082731 1 BBa_K395007 component2082748 1 BBa_J54103 annotation2082731 1 BBa_K395007 range2082731 1 1 30 annotation2082748 1 BBa_J54103 range2082748 1 39 969 BBa_K395007 1 BBa_K395007 plux(rep)-Green 2010-10-04T11:00:00Z 2015-05-08T01:12:21Z test Released HQ 2013 repressed by protein LuxR and 3OC6HSL. have the same consensus sequence of -35 and -10 as plac. quoted Kristi A. Egland and E. P. Greenberg. 1999.Conversion of the Vibrio fischeri Transcriptional Activator, LuxR, to a Repressor. J.Bacteriol. 182:805???811. true false _505_ 0 6593 9 Discontinued false test test false Eriko Uchikoshi BBa_K395104_sequence 1 ttgacactttatgcttccggctcgtataattactagaggtcgactgacgactggatcctatcaagatctgtcggataacgcgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K395007_sequence 1 ttgacacctgtaggatcgtacaggtataat BBa_R0061_sequence 1 ttgacacctgtaggatcgtacaggtataat BBa_J54103_sequence 1 gtcgactgacgactggatcctgtcggagatctgtcggataacgcgtgagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z