BBa_J54103
1
BBa_J54103
Promoterless-GFP(A)
2006-10-16T11:00:00Z
2015-08-31T03:48:11Z
synthesis
Released HQ 2013
promoterless
false
true
_76_
0
762
76
In stock
true
as negative control
true
Yusaku Nakashima
annotation2152491
1
BamHI
range2152491
1
15
20
annotation2152501
1
stem_loop
range2152501
1
811
854
annotation2152494
1
MulI
range2152494
1
41
46
annotation2152495
1
RBS-1\Strong
range2152495
1
49
64
annotation2152490
1
SalI
range2152490
1
1
6
annotation2152502
1
T7 TE
range2152502
1
899
918
annotation2152497
1
RBS
range2152497
1
56
62
annotation2152492
1
BBa_J54025
range2152492
1
27
46
annotation2152498
1
BBa_E0040
range2152498
1
71
790
annotation2152496
1
BBa_B0030
range2152496
1
49
64
annotation2152500
1
BBa_B0012
range2152500
1
887
927
annotation2152499
1
BBa_B0010
range2152499
1
800
879
annotation2152493
1
BglII
range2152493
1
27
32
annotation2152489
1
BBa_J54033
range2152489
1
1
20
BBa_R0071
1
RhlR+C4
Promoter (RhlR & C4-HSL regulated)
2004-01-24T12:00:00Z
2015-05-08T01:14:15Z
<i>Pseudomonas aeruginosa</i> rhlAB promoter
Released HQ 2013
Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer.
<p>
This is the natural sequence taken from Pseudomonas aeruginosa.
<p>
Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
false
false
_1_
0
24
7
In stock
false
The -10 and -35 sites are labeled as identified in [Pearson97].
<P>
The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
true
Ronny Krashinsky
annotation297104
1
-10
range297104
1
42
47
annotation297102
1
RhlR
range297102
1
1
20
annotation297105
1
start
range297105
1
53
53
annotation297103
1
-35
range297103
1
19
24
BBa_K395107
1
BBa_K395107
GFP reporter activated by RhlR and C4HSL (R0071:K121013)
2010-10-04T11:00:00Z
2015-05-08T01:12:21Z
We ligated BBa_R0071 into BBa_K121013.
This part is GFP reporter which is represed by RhlR and C4HSL, and the promoter is R0071. And we use this part to measure the activity of BBa_R0071.
false
false
_505_
0
6370
9
Not in stock
false
yes
false
Shohei Kitano
component2082806
1
BBa_J54103
component2082789
1
BBa_R0071
annotation2082789
1
BBa_R0071
range2082789
1
1
53
annotation2082806
1
BBa_J54103
range2082806
1
62
992
BBa_R0071_sequence
1
tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttc
BBa_K395107_sequence
1
tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagaggtcgactgacgactggatcctatcaagatctgtcggataacgcgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J54103_sequence
1
gtcgactgacgactggatcctgtcggagatctgtcggataacgcgtgagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z