BBa_K395107 1 BBa_K395107 GFP reporter activated by RhlR and C4HSL (R0071:K121013) 2010-10-04T11:00:00Z 2015-05-08T01:12:21Z We ligated BBa_R0071 into BBa_K121013. This part is GFP reporter which is represed by RhlR and C4HSL, and the promoter is R0071. And we use this part to measure the activity of BBa_R0071. false false _505_ 0 6370 9 Not in stock false yes false Shohei Kitano component2082789 1 BBa_R0071 component2082806 1 BBa_J54103 annotation2082806 1 BBa_J54103 range2082806 1 62 992 annotation2082789 1 BBa_R0071 range2082789 1 1 53 BBa_J54103 1 BBa_J54103 Promoterless-GFP(A) 2006-10-16T11:00:00Z 2015-08-31T03:48:11Z synthesis Released HQ 2013 promoterless false true _76_ 0 762 76 In stock true as negative control true Yusaku Nakashima annotation2152491 1 BamHI range2152491 1 15 20 annotation2152495 1 RBS-1\Strong range2152495 1 49 64 annotation2152502 1 T7 TE range2152502 1 899 918 annotation2152492 1 BBa_J54025 range2152492 1 27 46 annotation2152497 1 RBS range2152497 1 56 62 annotation2152490 1 SalI range2152490 1 1 6 annotation2152498 1 BBa_E0040 range2152498 1 71 790 annotation2152496 1 BBa_B0030 range2152496 1 49 64 annotation2152501 1 stem_loop range2152501 1 811 854 annotation2152489 1 BBa_J54033 range2152489 1 1 20 annotation2152499 1 BBa_B0010 range2152499 1 800 879 annotation2152493 1 BglII range2152493 1 27 32 annotation2152500 1 BBa_B0012 range2152500 1 887 927 annotation2152494 1 MulI range2152494 1 41 46 BBa_R0071 1 RhlR+C4 Promoter (RhlR & C4-HSL regulated) 2004-01-24T12:00:00Z 2015-05-08T01:14:15Z <i>Pseudomonas aeruginosa</i> rhlAB promoter Released HQ 2013 Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer. <p> This is the natural sequence taken from Pseudomonas aeruginosa. <p> Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL. false false _1_ 0 24 7 In stock false The -10 and -35 sites are labeled as identified in [Pearson97]. <P> The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1). true Ronny Krashinsky annotation297104 1 -10 range297104 1 42 47 annotation297105 1 start range297105 1 53 53 annotation297103 1 -35 range297103 1 19 24 annotation297102 1 RhlR range297102 1 1 20 BBa_R0071_sequence 1 tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttc BBa_K395107_sequence 1 tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagaggtcgactgacgactggatcctatcaagatctgtcggataacgcgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_J54103_sequence 1 gtcgactgacgactggatcctgtcggagatctgtcggataacgcgtgagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z