BBa_K395025
1
BBa_K395025
plas(rep)
2010-10-04T11:00:00Z
2015-05-08T01:12:21Z
test
Repressed by LasR and 3OC12HSL. Have the same consensus sequence of -35 and -10 as that of R0061 which repressed LasR and 3OC12HSL, and was inserted LasR binding site between the sequence of -35 and -10.
false
false
_505_
0
6593
9
Not in stock
false
test test
false
Eriko Uchikoshi
BBa_K395110
1
BBa_K395110
GFP reporter repressed by LasR and 3OC12HSL (K395025:K121013)
2010-10-04T11:00:00Z
2015-05-08T01:12:21Z
We ligated BBa_K395025 into BBa_K121013.
This part is GFP reporter which is repressed by LasR and 3OC12HSL, and the promoter is K395025. And we use this part to measure the activity of BBa_K395025.
false
false
_505_
0
6370
9
Not in stock
false
yes
false
Shohei Kitano
component2082826
1
BBa_K395025
component2082843
1
BBa_J54103
annotation2082826
1
BBa_K395025
range2082826
1
1
30
annotation2082843
1
BBa_J54103
range2082843
1
39
969
BBa_J54103
1
BBa_J54103
Promoterless-GFP(A)
2006-10-16T11:00:00Z
2015-08-31T03:48:11Z
synthesis
Released HQ 2013
promoterless
false
true
_76_
0
762
76
In stock
true
as negative control
true
Yusaku Nakashima
annotation2152491
1
BamHI
range2152491
1
15
20
annotation2152490
1
SalI
range2152490
1
1
6
annotation2152502
1
T7 TE
range2152502
1
899
918
annotation2152493
1
BglII
range2152493
1
27
32
annotation2152497
1
RBS
range2152497
1
56
62
annotation2152499
1
BBa_B0010
range2152499
1
800
879
annotation2152498
1
BBa_E0040
range2152498
1
71
790
annotation2152494
1
MulI
range2152494
1
41
46
annotation2152500
1
BBa_B0012
range2152500
1
887
927
annotation2152492
1
BBa_J54025
range2152492
1
27
46
annotation2152495
1
RBS-1\Strong
range2152495
1
49
64
annotation2152496
1
BBa_B0030
range2152496
1
49
64
annotation2152501
1
stem_loop
range2152501
1
811
854
annotation2152489
1
BBa_J54033
range2152489
1
1
20
BBa_K395025_sequence
1
ttgacactgcccggaagggcaggttataat
BBa_J54103_sequence
1
gtcgactgacgactggatcctgtcggagatctgtcggataacgcgtgagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K395110_sequence
1
ttgacactgcccggaagggcaggttataattactagaggtcgactgacgactggatcctatcaagatctgtcggataacgcgttactagagattaaagaggagaaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z