BBa_K395161
1
BBa_K395161
RBS-KanR(no promoter and terminator)
2010-10-04T11:00:00Z
2015-05-08T01:12:21Z
We get this part ,RBS-KanR, from BBa_P1003 by PCR. this doesn't have any promoters and terminators.
false
false
_505_
0
6371
9
Not in stock
false
We want to create promoterless KanR on pSB6A1. so We get this part ,RBS-KanR, from BBa_P1003 by PCR and then ligated with pSB6A1
false
Kaneta Yusuke
annotation2082559
1
KanR
range2082559
1
16
835
BBa_K395163
1
BBa_K395163
KanR activated by RhlR and C4HSL(AHL of Rhl family)
2010-10-04T11:00:00Z
2015-05-08T01:12:21Z
this part express Kanamycin resistance by the activation of RhlR and C4HSL
false
false
_505_
0
6371
9
Not in stock
false
We want to create Kanamycin resistance gene on pSB6A1 activated by RhlR and C4HSL.As a vector,We already create Kanamycin resistance gene on pSB6A1and promotor exist as biobrick(Bba_R0071).To accomplish it, vector was cutted by EcoRI and Xha while insert(promotor) was cutted by EcoRI and SpeI
false
Kaneta Yusuke
component2082590
1
BBa_R0071
component2082592
1
BBa_K395161
annotation2082590
1
BBa_R0071
range2082590
1
1
53
annotation2082592
1
BBa_K395161
range2082592
1
62
896
BBa_R0071
1
RhlR+C4
Promoter (RhlR & C4-HSL regulated)
2004-01-24T12:00:00Z
2015-05-08T01:14:15Z
<i>Pseudomonas aeruginosa</i> rhlAB promoter
Released HQ 2013
Promoter activated by RhlR in concert with C4-HSL. C4-HSL is produced by RhlI, and acts as a quorum-sensing autoinducer.
<p>
This is the natural sequence taken from Pseudomonas aeruginosa.
<p>
Crosstalk: this promoter is also activated at a low level by LasR with its associated HSL.
false
false
_1_
0
24
7
In stock
false
The -10 and -35 sites are labeled as identified in [Pearson97].
<P>
The part begins with the RhlR binding site, and (rather arbitrarily) ends with the first transcribed base (+1).
true
Ronny Krashinsky
annotation297104
1
-10
range297104
1
42
47
annotation297102
1
RhlR
range297102
1
1
20
annotation297105
1
start
range297105
1
53
53
annotation297103
1
-35
range297103
1
19
24
BBa_R0071_sequence
1
tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttc
BBa_K395163_sequence
1
tcctgtgaaatctggcagttaccgttagctttcgaattggctaaaaagtgttctactagagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
BBa_K395161_sequence
1
taatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactccaacatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccagagttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatcaagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtgaaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctcaggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaagctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttggacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaatatggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z