##gff-version 3
##sequence-region BBa_K395170 1 987
BBa_K395170	.	engineered_region	46	987	.	+	0	ID=BBa_I0466;Name=BBa_I0466
BBa_K395170	.	terminator	859	938	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I0466
BBa_K395170	.	engineered_region	859	938	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K395170	.	stem_loop	870	913	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K395170	.	ribosome_entry_site	46	57	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I0466
BBa_K395170	.	conserved_region	50	53	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K395170	.	terminator	947	987	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I0466
BBa_K395170	.	engineered_region	947	987	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K395170	.	polyA_site	974	987	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K395170	.	stem_loop	954	973	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K395170	.	stop_codon	980	980	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K395170	.	CDS	64	825	.	+	0	ID=BBa_C0071;Name=BBa_C0071;Parent=BBa_I0466
BBa_K395170	.	engineered_tag	826	850	.	+	0	ID=annotation2213995;Name=Help:Barcodes;Parent=BBa_C0071
BBa_K395170	.	CDS	64	786	.	+	0	ID=annotation301192;Name=rhlR;Parent=BBa_C0071
BBa_K395170	.	tag	787	819	.	+	0	ID=annotation306523;Name=LVA;Parent=BBa_C0071
BBa_K395170	.	promoter	1	37	.	+	0	ID=BBa_I14032;Name=BBa_I14032
BBa_K395170	.	operator	1	37	.	+	0	ID=annotation1028342;Name=P(Lac) IQ;Parent=BBa_I14032
BBa_K395170	.	promoter	26	31	.	+	0	ID=annotation1028344;Name=-10;Parent=BBa_I14032
BBa_K395170	.	promoter	3	8	.	+	0	ID=annotation1028343;Name=-35;Parent=BBa_I14032
>BBa_K395170
tggtgcaaaacctttcgcggtatggcatgatagcgcctactagagaaagaggagaaatactagatgaggaatgacggag
gctttttgctgtggtgggacggtttgcgtagcgagatgcagccgatccacgacagccagggcgtgttcgccgtcctgga
aaaggaagtgcggcgcctgggcttcgattactacgcctatggcgtgcgccacacgattcccttcacccggccgaagacc
gaggtccatggcacctatcccaaggcctggctggagcgataccagatgcagaactacggggccgtggatccggcgatcc
tcaacggcctgcgctcctcggaaatggtggtctggagcgacagcctgttcgaccagagccggatgctctggaacgaggc
tcgcgattggggcctctgtgtcggcgcgaccttgccgatccgcgcgccgaacaatttgctcagcgtgctttccgtggcg
cgcgaccagcagaacatctccagcttcgagcgcgaggaaatccgcctgcggctgcgttgcatgatcgagttgctgaccc
agaagctgaccgacctggagcatccgatgctgatgtccaacccggtctgcctgagccatcgcgaacgcgagatcctgca
atggaccgccgacggcaagagttccggggaaatcgccatcatcctgagcatctccgagagcacggtgaacttccaccac
aagaacatccagaagaagttcgacgcgccgaacaagacgctggctgccgcctacgccgcggcgctgggtctcatcgctg
caaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaa
ataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtc
acactggctcaccttcgggtgggcctttctgcgtttata