LOCUS       BBa_K395304              947 bp    DNA     linear       15-May-2024
DEFINITION  PompC(C) with GFP reporter (K395301+E0240)
ACCESSION   BBa_K395304
VERSION     BBa_K395304.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Binding site 1 
     promoter        30..35
                     /label=-35
     promoter        53..58
                     /label=-10
     promoter        1..63
                     /label=BBa_K395301
     RBS             72..84
                     /label=RBS-3\Weak
     RBS             78..81
                     /label=RBS
     misc_feature    72..84
                     /label=BBa_B0032
     RBS             72..84
                     /label=BBa_B0032
     CDS             91..810
                     /label=GFP protein
     CDS             91..810
                     /label=BBa_E0040
     stem_loop       830..873
                     /label=stem_loop
     misc_feature    819..898
                     /label=BBa_B0010
     terminator      819..898
                     /label=BBa_B0010
     stem_loop       914..933
                     /label=T7 TE
     misc_feature    940..940
                     /label=stop
     polyA_site      934..947
                     /label=polya
     misc_feature    907..947
                     /label=BBa_B0012
     terminator      907..947
                     /label=BBa_B0012
ORIGIN
        1 atttacattt tgaaacatct gacctcgagt tggattattc tgcatttttg gggagaatgg
       61 acttactaga gtcacacagg aaagtactag atgcgtaaag gagaagaact tttcactgga
      121 gttgtcccaa ttcttgttga attagatggt gatgttaatg ggcacaaatt ttctgtcagt
      181 ggagagggtg aaggtgatgc aacatacgga aaacttaccc ttaaatttat ttgcactact
      241 ggaaaactac ctgttccatg gccaacactt gtcactactt tcggttatgg tgttcaatgc
      301 tttgcgagat acccagatca tatgaaacag catgactttt tcaagagtgc catgcccgaa
      361 ggttatgtac aggaaagaac tatatttttc aaagatgacg ggaactacaa gacacgtgct
      421 gaagtcaagt ttgaaggtga tacccttgtt aatagaatcg agttaaaagg tattgatttt
      481 aaagaagatg gaaacattct tggacacaaa ttggaataca actataactc acacaatgta
      541 tacatcatgg cagacaaaca aaagaatgga atcaaagtta acttcaaaat tagacacaac
      601 attgaagatg gaagcgttca actagcagac cattatcaac aaaatactcc aattggcgat
      661 ggccctgtcc ttttaccaga caaccattac ctgtccacac aatctgccct ttcgaaagat
      721 cccaacgaaa agagagacca catggtcctt cttgagtttg taacagctgc tgggattaca
      781 catggcatgg atgaactata caaataataa tactagagcc aggcatcaaa taaaacgaaa
      841 ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta
      901 ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//