LOCUS       BBa_K395305              953 bp    DNA     linear       01-Jun-2024
DEFINITION  PompC(CB) with GFP reporter  (K395302+E0240)
ACCESSION   BBa_K395305
VERSION     BBa_K395305.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=Binding site 1
     promoter        36..41
                     /label=-35
     promoter        59..64
                     /label=-10
     promoter        1..69
                     /label=BBa_K395302
     RBS             78..90
                     /label=RBS-3\Weak
     RBS             84..87
                     /label=RBS
     misc_feature    78..90
                     /label=BBa_B0032
     RBS             78..90
                     /label=BBa_B0032
     CDS             97..816
                     /label=GFP protein
     CDS             97..816
                     /label=BBa_E0040
     stem_loop       836..879
                     /label=stem_loop
     misc_feature    825..904
                     /label=BBa_B0010
     terminator      825..904
                     /label=BBa_B0010
     stem_loop       920..939
                     /label=T7 TE
     misc_feature    946..946
                     /label=stop
     polyA_site      940..953
                     /label=polya
     misc_feature    913..953
                     /label=BBa_B0012
     terminator      913..953
                     /label=BBa_B0012
     misc_feature    78..953
                     /label=BBa_E0240
ORIGIN
        1 atttacattt tgaaacatct gaccccggat ccgggttgga ttattctgca tttttgggga
       61 gaatggactt actagagtca cacaggaaag tactagatgc gtaaaggaga agaacttttc
      121 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
      181 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
      241 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
      301 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
      361 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
      421 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
      481 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
      541 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
      601 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
      661 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
      721 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
      781 attacacatg gcatggatga actatacaaa taataatact agagccaggc atcaaataaa
      841 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
      901 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//