LOCUS       BBa_K395401             2456 bp    DNA     linear       30-Jun-2024
DEFINITION  pBad/araC-lacIM1
ACCESSION   BBa_K395401
VERSION     BBa_K395401.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1223..1226
                     /label=conserved
     RBS             1219..1230
                     /label=BBa_B0034
     misc_feature    1237..2319
                     /label=BBa_K082005
     CDS             1237..2319
                     /label=BBa_K082005
     stem_loop       2339..2382
                     /label=stem_loop
     misc_feature    2328..2407
                     /label=BBa_B0010
     terminator      2328..2407
                     /label=BBa_B0010
     stem_loop       2423..2442
                     /label=T7 TE
     misc_feature    2449..2449
                     /label=stop
     polyA_site      2443..2456
                     /label=polya
     misc_feature    2416..2456
                     /label=BBa_B0012
     terminator      2416..2456
                     /label=BBa_B0012
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc tactagagaa agaggagaaa tactagatga agccggtgac cctgtatgac
     1261 gtggcggaat acgcgggcgt gagctaccaa acggtgagcc gtgttgttaa tcaagcgtct
     1321 catgtgagcg ccaagacccg cgagaaggtt gaggccgcca tggccgaatt aaactatatc
     1381 ccgaatcgtg ttgcgcagca gttagccggt aagcaaagcc tgttaatcgg tgtggcgacg
     1441 agcagcttag cgtcacatgc cccaagccag atcgtggccg ccatcaagag ccgtgcggac
     1501 cagttaggcg cgtctgttgt tgttagaatg gtggagcgct ctggtgtgga ggcgtgcaag
     1561 gccgccgttc ataacctgct ggcccagcgt gtgagcggct taattatcaa ttacccatta
     1621 gacgatcaag acgcgatcgc ggttgaggcg gcgtgtacca acgtgccagc cctgttcctg
     1681 gacgtgagcg atcaaacccc gattaatagc atcatcttta gccacgagaa tggcacccgc
     1741 ttaggtgttg aacacttagt ggccctgggc catcaacaga ttgccctact ggccggtccg
     1801 ctgagcagcg tgagcgcccg cttacgctta gcgggttggc acaagtacct gacccgtaac
     1861 cagatccaac caattgccga gcgcgagggt gattggagcg cgatgagcgg cttccagcag
     1921 acgatgcaga tgttaaacga aggtattgtg ccgaccgcca tgttagtggc gaatgaccaa
     1981 atggccttag gtgcgatgcg tgcgatcacg gaaagcggct tacgtgtggg cgccgacatt
     2041 agcgtggttg gctatgatga cacggaggat tctagctgct acattccacc aagcacgacg
     2101 attaagcaag acttccgttt attaggccag acgtctgttg atcgtctgtt acagctgagc
     2161 caaggtcaag ccgttaaagg taaccaatta ctgccggtga gcttagttaa gcgcaagacg
     2221 acgttagccc cgaacaccca gacggcgagc ccgcgtgccc tggcggacag cctgatgcaa
     2281 ttagcgcgcc aagtgagccg cttagagtct ggccaatgat actagagcca ggcatcaaat
     2341 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     2401 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//