LOCUS BBa_K395403 3321 bp DNA linear 17-May-2024 DEFINITION pBad-ｃI-pλ-lacI-lacI+pL-GFP ACCESSION BBa_K395403 VERSION BBa_K395403.1 FEATURES Location/Qualifiers promoter 1..130 /label=BBa_I13453 misc_feature 143..146 /label=conserved RBS 139..150 /label=BBa_B0034 CDS 160..867 /label=cI lambda misc_feature 868..900 /label=LVA misc_feature 907..931 /label=Help:Barcodes CDS 157..906 /label=BBa_C0051 stem_loop 951..994 /label=stem_loop misc_feature 940..1019 /label=BBa_B0010 terminator 940..1019 /label=BBa_B0010 stem_loop 1035..1054 /label=T7 TE misc_feature 1061..1061 /label=stop polyA_site 1055..1068 /label=polya misc_feature 1028..1068 /label=BBa_B0012 terminator 1028..1068 /label=BBa_B0012 misc_feature 139..1068 /label=BBa_P0451 promoter 1091..1096 /label=-35 promoter 1101..1117 /label=OR1 promoter 1077..1093 /label=OR2 promoter 1114..1119 /label=-10 misc_feature 1077..1125 /label=BBa_R1051 promoter 1077..1125 /label=BBa_R1051 misc_feature 1138..1141 /label=conserved RBS 1134..1145 /label=BBa_B0034 CDS 1152..2237 /label=BBa_I732100 stem_loop 2257..2300 /label=stem_loop misc_feature 2246..2325 /label=BBa_B0010 terminator 2246..2325 /label=BBa_B0010 stem_loop 2341..2360 /label=T7 TE misc_feature 2367..2367 /label=stop polyA_site 2361..2374 /label=polya misc_feature 2334..2374 /label=BBa_B0012 terminator 2334..2374 /label=BBa_B0012 misc_feature 1134..2374 /label=BBa_I732820 misc_feature 2385..2401 /label=lac O1 promoter 2402..2407 /label=-35 misc_feature 2408..2424 /label=lac O1 promoter 2425..2430 /label=-10 misc_feature 2383..2436 /label=BBa_R0011 promoter 2383..2436 /label=BBa_R0011 RBS 2446..2458 /label=RBS-3\Weak RBS 2452..2455 /label=RBS misc_feature 2446..2458 /label=BBa_B0032 RBS 2446..2458 /label=BBa_B0032 CDS 2465..3184 /label=GFP protein CDS 2465..3184 /label=BBa_E0040 stem_loop 3204..3247 /label=stem_loop misc_feature 3193..3272 /label=BBa_B0010 terminator 3193..3272 /label=BBa_B0010 stem_loop 3288..3307 /label=T7 TE misc_feature 3314..3314 /label=stop polyA_site 3308..3321 /label=polya misc_feature 3281..3321 /label=BBa_B0012 terminator 3281..3321 /label=BBa_B0012 misc_feature 2446..3321 /label=BBa_E0240 ORIGIN 1 acattgatta tttgcacggc gtcacacttt gctatgccat agcattttta tccataagat 61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt 121 ttgggctagc tactagagaa agaggagaaa tactagatga gcacaaaaaa gaaaccatta 181 acacaagagc agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat 241 gaacttggct tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt 301 ggtgctttat ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa 361 attctcaaag ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg 421 tatgaagcgg ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct 481 catgttcagg cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag 541 agatgggtaa gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt 601 aattccatga ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc 661 gttgaccctg agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat 721 gagtttacct tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac 781 ccacagtacc caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct 841 agtcagtggc ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct 901 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa 961 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct 1021 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaac 1081 accgtgcgtg ttgacaattt tacctctggc ggtgatactg gttgctacta gagaaagagg 1141 agaaatacta gatgaaacca gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt 1201 atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa 1261 aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca caacaactgg 1321 cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg cacgcgccgt 1381 cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt 1441 cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc 1501 aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc attgctgtgg 1561 aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag acacccatca 1621 acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat ctggtcgcat 1681 tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc 1741 gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata gcggaacggg 1801 aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg aatgagggca 1861 tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca atgcgcgcca 1921 ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac gacgataccg 1981 aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg 2041 ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc 2101 agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg 2161 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg 2221 aaagcgggca gtaataatac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa 2281 gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact 2341 ggctcacctt cgggtgggcc tttctgcgtt tatatactag agaattgtga gcggataaca 2401 attgacattg tgagcggata acaagatact gagcacatac tagagtcaca caggaaagta 2461 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga 2521 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata 2581 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac 2641 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa 2701 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt 2761 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct 2821 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca 2881 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa 2941 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc 3001 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca 3061 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt 3121 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata 3181 ataatactag agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc 3241 gttttatctg ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg 3301 gtgggccttt ctgcgtttat a //