##gff-version 3
##sequence-region BBa_K395403 1 3321
BBa_K395403	.	engineered_region	139	1068	.	+	0	ID=BBa_P0451;Name=BBa_P0451
BBa_K395403	.	terminator	1028	1068	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0451
BBa_K395403	.	engineered_region	1028	1068	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K395403	.	stop_codon	1061	1061	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K395403	.	stem_loop	1035	1054	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K395403	.	polyA_site	1055	1068	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K395403	.	terminator	940	1019	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0451
BBa_K395403	.	engineered_region	940	1019	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K395403	.	stem_loop	951	994	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K395403	.	ribosome_entry_site	139	150	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0451
BBa_K395403	.	conserved_region	143	146	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K395403	.	CDS	157	906	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0451
BBa_K395403	.	tag	868	900	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K395403	.	CDS	160	867	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K395403	.	engineered_tag	907	931	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K395403	.	promoter	2383	2436	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K395403	.	promoter	2402	2407	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K395403	.	promoter	2425	2430	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K395403	.	operator	2408	2424	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K395403	.	operator	2385	2401	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K395403	.	engineered_region	2383	2436	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K395403	.	promoter	1077	1125	.	+	0	ID=BBa_R1051;Name=BBa_R1051
BBa_K395403	.	promoter	1101	1117	.	+	0	ID=annotation2076;Name=OR1;Parent=BBa_R1051
BBa_K395403	.	promoter	1077	1093	.	+	0	ID=annotation2077;Name=OR2;Parent=BBa_R1051
BBa_K395403	.	promoter	1091	1096	.	+	0	ID=annotation2075;Name=-35;Parent=BBa_R1051
BBa_K395403	.	engineered_region	1077	1125	.	+	0	ID=annotation7074;Name=BBa_R1051;Parent=BBa_R1051
BBa_K395403	.	promoter	1114	1119	.	+	0	ID=annotation2078;Name=-10;Parent=BBa_R1051
BBa_K395403	.	engineered_region	2446	3321	.	+	0	ID=BBa_E0240;Name=BBa_E0240
BBa_K395403	.	terminator	3193	3272	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_E0240
BBa_K395403	.	engineered_region	3193	3272	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K395403	.	stem_loop	3204	3247	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K395403	.	CDS	2465	3184	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0240
BBa_K395403	.	CDS	2465	3184	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K395403	.	ribosome_entry_site	2446	2458	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0240
BBa_K395403	.	ribosome_entry_site	2452	2455	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K395403	.	ribosome_entry_site	2446	2458	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K395403	.	engineered_region	2446	2458	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K395403	.	terminator	3281	3321	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_E0240
BBa_K395403	.	engineered_region	3281	3321	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K395403	.	stop_codon	3314	3314	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K395403	.	stem_loop	3288	3307	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K395403	.	polyA_site	3308	3321	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K395403	.	engineered_region	1134	2374	.	+	0	ID=BBa_I732820;Name=BBa_I732820
BBa_K395403	.	ribosome_entry_site	1134	1145	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732820
BBa_K395403	.	conserved_region	1138	1141	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K395403	.	terminator	2334	2374	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732820
BBa_K395403	.	engineered_region	2334	2374	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K395403	.	stop_codon	2367	2367	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K395403	.	stem_loop	2341	2360	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K395403	.	polyA_site	2361	2374	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K395403	.	CDS	1152	2237	.	+	0	ID=BBa_I732100;Name=BBa_I732100;Parent=BBa_I732820
BBa_K395403	.	terminator	2246	2325	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732820
BBa_K395403	.	engineered_region	2246	2325	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K395403	.	stem_loop	2257	2300	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K395403	.	promoter	1	130	.	+	0	ID=BBa_I13453;Name=BBa_I13453
>BBa_K395403
acattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacg
ctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagaaagaggagaaatactagat
gagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaa
aatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatg
gcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttc
aatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgtt
ttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagca
caaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagcc
aagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagactt
gggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacc
caatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgc
tgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatc
aaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagag
tcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgacaattttacctc
tggcggtgatactggttgctactagagaaagaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagt
atgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagt
ggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgatt
ggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgg
gtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgca
acgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgtt
ccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgg
gcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtct
gcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgcc
atgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcaga
tggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacga
taccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtg
gaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaa
ccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttc
ccgactggaaagcgggcagtaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tatatactagagaattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcac
acaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatg
ttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttg
cactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatac
ccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttca
aagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaagg
tattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatg
gcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcag
accattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgc
cctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggc
atggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggccttt
cgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgttt
ata