BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_K395301 1 PompC(C) PompC(C) Promoter regulated by phosphorylated OmpR 2010-10-01T11:00:00Z 2015-05-08T01:12:21Z ATTTACATTTTGAAACATCTGACCTCGAGTTGGATTATTCTGCATTTTTGGGGAGAATGGACT Promoter regulated by phosphorylated OmpR false false _505_ 0 7028 9 Not in stock false A false THIPRAMPAI THAMAMONGOOD annotation2102138 1 -35 range2102138 1 30 35 annotation2102140 1 -10 range2102140 1 53 58 annotation2102132 1 Binding site 1 range2102132 1 1 20 BBa_K082005 1 BBa_K082005 LacI-Mutant 2008-10-27T12:00:00Z 2015-05-08T01:08:35Z None None false false _228_ 0 1560 9 It's complicated false None false Yanting Xue, Binjie Xu annotation1992781 1 BBa_K082005 range1992781 1 1 1083 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K395405 1 BBa_K395405 pompC(C)-lacIM1 2010-10-04T11:00:00Z 2015-05-08T01:12:21Z test lacIM1 regulated by ompC-C promoter.The promoter is regulated by phosphorylated OmpR. false false _505_ 0 6396 9 Not in stock false test false Mitsuhiko Odera component2082476 1 BBa_B0012 component2082474 1 BBa_B0010 component2082473 1 BBa_K082005 component2082472 1 BBa_B0034 component2082470 1 BBa_K395301 annotation2082473 1 BBa_K082005 range2082473 1 90 1172 annotation2082476 1 BBa_B0012 range2082476 1 1269 1309 annotation2082470 1 BBa_K395301 range2082470 1 1 63 annotation2082472 1 BBa_B0034 range2082472 1 72 83 annotation2082474 1 BBa_B0010 range2082474 1 1181 1260 BBa_K395405_sequence 1 atttacattttgaaacatctgacctcgagttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatgaagccggtgaccctgtatgacgtggcggaatacgcgggcgtgagctaccaaacggtgagccgtgttgttaatcaagcgtctcatgtgagcgccaagacccgcgagaaggttgaggccgccatggccgaattaaactatatcccgaatcgtgttgcgcagcagttagccggtaagcaaagcctgttaatcggtgtggcgacgagcagcttagcgtcacatgccccaagccagatcgtggccgccatcaagagccgtgcggaccagttaggcgcgtctgttgttgttagaatggtggagcgctctggtgtggaggcgtgcaaggccgccgttcataacctgctggcccagcgtgtgagcggcttaattatcaattacccattagacgatcaagacgcgatcgcggttgaggcggcgtgtaccaacgtgccagccctgttcctggacgtgagcgatcaaaccccgattaatagcatcatctttagccacgagaatggcacccgcttaggtgttgaacacttagtggccctgggccatcaacagattgccctactggccggtccgctgagcagcgtgagcgcccgcttacgcttagcgggttggcacaagtacctgacccgtaaccagatccaaccaattgccgagcgcgagggtgattggagcgcgatgagcggcttccagcagacgatgcagatgttaaacgaaggtattgtgccgaccgccatgttagtggcgaatgaccaaatggccttaggtgcgatgcgtgcgatcacggaaagcggcttacgtgtgggcgccgacattagcgtggttggctatgatgacacggaggattctagctgctacattccaccaagcacgacgattaagcaagacttccgtttattaggccagacgtctgttgatcgtctgttacagctgagccaaggtcaagccgttaaaggtaaccaattactgccggtgagcttagttaagcgcaagacgacgttagccccgaacacccagacggcgagcccgcgtgccctggcggacagcctgatgcaattagcgcgccaagtgagccgcttagagtctggccaatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_B0034_sequence 1 aaagaggagaaa BBa_K082005_sequence 1 atgaagccggtgaccctgtatgacgtggcggaatacgcgggcgtgagctaccaaacggtgagccgtgttgttaatcaagcgtctcatgtgagcgccaagacccgcgagaaggttgaggccgccatggccgaattaaactatatcccgaatcgtgttgcgcagcagttagccggtaagcaaagcctgttaatcggtgtggcgacgagcagcttagcgtcacatgccccaagccagatcgtggccgccatcaagagccgtgcggaccagttaggcgcgtctgttgttgttagaatggtggagcgctctggtgtggaggcgtgcaaggccgccgttcataacctgctggcccagcgtgtgagcggcttaattatcaattacccattagacgatcaagacgcgatcgcggttgaggcggcgtgtaccaacgtgccagccctgttcctggacgtgagcgatcaaaccccgattaatagcatcatctttagccacgagaatggcacccgcttaggtgttgaacacttagtggccctgggccatcaacagattgccctactggccggtccgctgagcagcgtgagcgcccgcttacgcttagcgggttggcacaagtacctgacccgtaaccagatccaaccaattgccgagcgcgagggtgattggagcgcgatgagcggcttccagcagacgatgcagatgttaaacgaaggtattgtgccgaccgccatgttagtggcgaatgaccaaatggccttaggtgcgatgcgtgcgatcacggaaagcggcttacgtgtgggcgccgacattagcgtggttggctatgatgacacggaggattctagctgctacattccaccaagcacgacgattaagcaagacttccgtttattaggccagacgtctgttgatcgtctgttacagctgagccaaggtcaagccgttaaaggtaaccaattactgccggtgagcttagttaagcgcaagacgacgttagccccgaacacccagacggcgagcccgcgtgccctggcggacagcctgatgcaattagcgcgccaagtgagccgcttagagtctggccaatga BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K395301_sequence 1 atttacattttgaaacatctgacctcgagttggattattctgcatttttggggagaatggact igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z