LOCUS BBa_K395408 3301 bp DNA linear 25-Nov-2024 DEFINITION pompC(WT)-cI-pλ-lacI-lacI+pL-GFP ACCESSION BBa_K395408 VERSION BBa_K395408.1 FEATURES Location/Qualifiers misc_feature 13..30 /label=C1 OmpR misc_feature 34..51 /label=C2 OmpR misc_feature 54..71 /label=C3 OmpR promoter 75..80 /label=-35 promoter 98..103 /label=-10 promoter 1..108 /label=BBa_R0082 misc_feature 121..124 /label=conserved RBS 117..128 /label=BBa_B0034 CDS 138..845 /label=cI lambda misc_feature 846..878 /label=LVA misc_feature 885..909 /label=Help:Barcodes CDS 135..884 /label=BBa_C0051 stem_loop 929..972 /label=stem_loop misc_feature 918..997 /label=BBa_B0010 terminator 918..997 /label=BBa_B0010 stem_loop 1013..1032 /label=T7 TE misc_feature 1039..1039 /label=stop polyA_site 1033..1046 /label=polya misc_feature 1006..1046 /label=BBa_B0012 terminator 1006..1046 /label=BBa_B0012 misc_feature 117..1046 /label=BBa_P0451 promoter 1069..1074 /label=-35 promoter 1079..1095 /label=OR1 promoter 1055..1071 /label=OR2 promoter 1092..1097 /label=-10 misc_feature 1055..1103 /label=BBa_R1051 promoter 1055..1103 /label=BBa_R1051 misc_feature 1116..1119 /label=conserved RBS 1112..1123 /label=BBa_B0034 CDS 1130..2215 /label=BBa_I732100 stem_loop 2235..2278 /label=stem_loop misc_feature 2224..2303 /label=BBa_B0010 terminator 2224..2303 /label=BBa_B0010 stem_loop 2319..2338 /label=T7 TE misc_feature 2345..2345 /label=stop polyA_site 2339..2352 /label=polya misc_feature 2312..2352 /label=BBa_B0012 terminator 2312..2352 /label=BBa_B0012 misc_feature 1112..2352 /label=BBa_I732820 misc_feature 2363..2379 /label=lac O1 promoter 2380..2385 /label=-35 misc_feature 2386..2402 /label=lac O1 promoter 2403..2408 /label=-10 misc_feature 2361..2414 /label=BBa_R0011 promoter 2361..2414 /label=BBa_R0011 RBS 2424..2438 /label=RBS-1\Strong RBS 2431..2435 /label=RBS misc_feature 2424..2438 /label=BBa_B0030 RBS 2424..2438 /label=BBa_B0030 CDS 2445..3164 /label=GFP protein CDS 2445..3164 /label=BBa_E0040 stem_loop 3184..3227 /label=stem_loop misc_feature 3173..3252 /label=BBa_B0010 terminator 3173..3252 /label=BBa_B0010 stem_loop 3268..3287 /label=T7 TE misc_feature 3294..3294 /label=stop polyA_site 3288..3301 /label=polya misc_feature 3261..3301 /label=BBa_B0012 terminator 3261..3301 /label=BBa_B0012 terminator 3173..3301 /label=BBa_B0015 ORIGIN 1 tcccttgcat ttacattttg aaacatctat agcgataaat gaaacatctt aaaagtttta 61 gtatcatatt cgtgttggat tattctgcat ttttggggag aatggactta ctagagaaag 121 aggagaaata ctagatgagc acaaaaaaga aaccattaac acaagagcag cttgaggacg 181 cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga acttggctta tcccaggaat 241 ctgtcgcaga caagatgggg atggggcagt caggcgttgg tgctttattt aatggcatca 301 atgcattaaa tgcttataac gccgcattgc ttgcaaaaat tctcaaagtt agcgttgaag 361 aatttagccc ttcaatcgcc agagaaatct acgagatgta tgaagcggtt agtatgcagc 421 cgtcacttag aagtgagtat gagtaccctg ttttttctca tgttcaggca gggatgttct 481 cacctgagct tagaaccttt accaaaggtg atgcggagag atgggtaagc acaaccaaaa 541 aagccagtga ttctgcattc tggcttgagg ttgaaggtaa ttccatgacc gcaccaacag 601 gctccaagcc aagctttcct gacggaatgt taattctcgt tgaccctgag caggctgttg 661 agccaggtga tttctgcata gccagacttg ggggtgatga gtttaccttc aagaaactga 721 tcagggatag cggtcaggtg tttttacaac cactaaaccc acagtaccca atgatcccat 781 gcaatgagag ttgttccgtt gtggggaaag ttatcgctag tcagtggcct gaagagacgt 841 ttggcgctgc aaacgacgaa aactacgctt tagtagctta ataacgctga tagtgctagt 901 gtagatcgct actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 961 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 1021 ttcgggtggg cctttctgcg tttatatact agagtaacac cgtgcgtgtt gacaatttta 1081 cctctggcgg tgatactggt tgctactaga gaaagaggag aaatactaga tgaaaccagt 1141 aacgttatac gatgtcgcag agtatgccgg tgtctcttat cagaccgttt cccgcgtggt 1201 gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa gtggaagcgg cgatggcgga 1261 gctgaattac attcccaacc gcgtggcaca acaactggcg ggcaaacagt cgttgctgat 1321 tggcgttgcc acctccagtc tggccctgca cgcgccgtcg caaattgtcg cggcgattaa 1381 atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg atggtagaac gaagcggcgt 1441 cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa cgcgtcagtg ggctgatcat 1501 taactatccg ctggatgacc aggatgccat tgctgtggaa gctgcctgca ctaatgttcc 1561 ggcgttattt cttgatgtct ctgaccagac acccatcaac agtattattt tctcccatga 1621 agacggtacg cgactgggcg tggagcatct ggtcgcattg ggtcaccagc aaatcgcgct 1681 gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt ctggctggct ggcataaata 1741 tctcactcgc aatcaaattc agccgatagc ggaacgggaa ggcgactgga gtgccatgtc 1801 cggttttcaa caaaccatgc aaatgctgaa tgagggcatc gttcccactg cgatgctggt 1861 tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt accgagtccg ggctgcgcgt 1921 tggtgcggat atctcggtag tgggatacga cgataccgaa gacagctcat gttatatccc 1981 gccgttaacc accatcaaac aggattttcg cctgctgggg caaaccagcg tggaccgctt 2041 gctgcaactc tctcagggcc aggcggtgaa gggcaatcag ctgttgcccg tctcactggt 2101 gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc tctccccgcg cgttggccga 2161 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt aataatacta 2221 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 2281 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 2341 tctgcgttta tatactagag aattgtgagc ggataacaat tgacattgtg agcggataac 2401 aagatactga gcacatacta gagattaaag aggagaaata ctagatgcgt aaaggagaag 2461 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca 2521 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat 2581 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt 2641 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga 2701 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact 2761 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa 2821 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata 2881 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca 2941 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata 3001 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg 3061 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag 3121 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agccaggcat 3181 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg 3241 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat 3301 a //