##gff-version 3
##sequence-region BBa_K395408 1 3301
BBa_K395408	.	engineered_region	117	1046	.	+	0	ID=BBa_P0451;Name=BBa_P0451
BBa_K395408	.	terminator	918	997	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0451
BBa_K395408	.	stem_loop	929	972	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K395408	.	engineered_region	918	997	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K395408	.	terminator	1006	1046	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0451
BBa_K395408	.	engineered_region	1006	1046	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K395408	.	stop_codon	1039	1039	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K395408	.	stem_loop	1013	1032	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K395408	.	polyA_site	1033	1046	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K395408	.	ribosome_entry_site	117	128	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0451
BBa_K395408	.	conserved_region	121	124	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K395408	.	CDS	135	884	.	+	0	ID=BBa_C0051;Name=BBa_C0051;Parent=BBa_P0451
BBa_K395408	.	tag	846	878	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K395408	.	CDS	138	845	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K395408	.	engineered_tag	885	909	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
BBa_K395408	.	ribosome_entry_site	2424	2438	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_K395408	.	engineered_region	2424	2438	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_K395408	.	ribosome_entry_site	2431	2435	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_K395408	.	ribosome_entry_site	2424	2438	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_K395408	.	promoter	1055	1103	.	+	0	ID=BBa_R1051;Name=BBa_R1051
BBa_K395408	.	promoter	1079	1095	.	+	0	ID=annotation2076;Name=OR1;Parent=BBa_R1051
BBa_K395408	.	promoter	1055	1071	.	+	0	ID=annotation2077;Name=OR2;Parent=BBa_R1051
BBa_K395408	.	promoter	1092	1097	.	+	0	ID=annotation2078;Name=-10;Parent=BBa_R1051
BBa_K395408	.	promoter	1069	1074	.	+	0	ID=annotation2075;Name=-35;Parent=BBa_R1051
BBa_K395408	.	engineered_region	1055	1103	.	+	0	ID=annotation7074;Name=BBa_R1051;Parent=BBa_R1051
BBa_K395408	.	terminator	3173	3301	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_K395408	.	terminator	3173	3252	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K395408	.	stem_loop	3184	3227	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K395408	.	engineered_region	3173	3252	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K395408	.	terminator	3261	3301	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K395408	.	engineered_region	3261	3301	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K395408	.	stop_codon	3294	3294	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K395408	.	stem_loop	3268	3287	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K395408	.	polyA_site	3288	3301	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K395408	.	promoter	1	108	.	+	0	ID=BBa_R0082;Name=BBa_R0082
BBa_K395408	.	promoter	98	103	.	+	0	ID=annotation301167;Name=-10;Parent=BBa_R0082
BBa_K395408	.	promoter	75	80	.	+	0	ID=annotation301166;Name=-35;Parent=BBa_R0082
BBa_K395408	.	non_covalent_binding_site	13	30	.	+	0	ID=annotation301154;Name=C1 OmpR;Parent=BBa_R0082
BBa_K395408	.	non_covalent_binding_site	34	51	.	+	0	ID=annotation301155;Name=C2 OmpR;Parent=BBa_R0082
BBa_K395408	.	non_covalent_binding_site	54	71	.	+	0	ID=annotation301156;Name=C3 OmpR;Parent=BBa_R0082
BBa_K395408	.	CDS	2445	3164	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K395408	.	CDS	2445	3164	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K395408	.	promoter	2361	2414	.	+	0	ID=BBa_R0011;Name=BBa_R0011
BBa_K395408	.	promoter	2380	2385	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K395408	.	promoter	2403	2408	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K395408	.	operator	2363	2379	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K395408	.	operator	2386	2402	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K395408	.	engineered_region	2361	2414	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K395408	.	engineered_region	1112	2352	.	+	0	ID=BBa_I732820;Name=BBa_I732820
BBa_K395408	.	terminator	2312	2352	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I732820
BBa_K395408	.	engineered_region	2312	2352	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K395408	.	stop_codon	2345	2345	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K395408	.	stem_loop	2319	2338	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K395408	.	polyA_site	2339	2352	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K395408	.	ribosome_entry_site	1112	1123	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I732820
BBa_K395408	.	conserved_region	1116	1119	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K395408	.	CDS	1130	2215	.	+	0	ID=BBa_I732100;Name=BBa_I732100;Parent=BBa_I732820
BBa_K395408	.	terminator	2224	2303	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I732820
BBa_K395408	.	stem_loop	2235	2278	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K395408	.	engineered_region	2224	2303	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K395408
tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttgga
ttattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatgagcacaaaaaagaaaccatta
acacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccagg
aatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgctta
taacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgag
atgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcaggga
tgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattc
tgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgtta
attctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttca
agaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagag
ttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgct
ttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtc
gaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggt
gggcctttctgcgtttatatactagagtaacaccgtgcgtgttgacaattttacctctggcggtgatactggttgctac
tagagaaagaggagaaatactagatgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagac
cgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctg
aattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctgg
ccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgat
ggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcatt
aactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtct
ctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcatt
gggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaa
tatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaacca
tgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgc
cattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttat
atcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctc
agggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgca
aaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaa
taatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcg
gtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagaattgtgagc
ggataacaattgacattgtgagcggataacaagatactgagcacatactagagattaaagaggagaaatactagatgcg
taaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttct
gtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctg
ttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagca
tgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaag
acacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatg
gaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatgg
aatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatact
ccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacg
aaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaata
ataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtc
ggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata