LOCUS       BBa_K395600             1757 bp    DNA     linear       25-Nov-2024
DEFINITION  Apple fragrance enzyme (MpAAT1) generator
ACCESSION   BBa_K395600
VERSION     BBa_K395600.1  
FEATURES             Location/Qualifiers
     misc_feature    5..49
                     /label=BBa_R0185
     promoter        6..22
                     /label=T7 promoter sequence
     misc_feature    25..49
                     /label=LacI binding site
     RBS             80..84
                     /label=rbs
     misc_feature    76..76
                     /label=start
     misc_feature    97..114
                     /label=his-tag
     misc_feature    148..181
                     /label=s-tag
     misc_feature    223..234
                     /label=factor-Xa
     CDS             235..1602
                     /label=MpAAT1
     misc_feature    1602..1602
                     /label=stop
     misc_feature    1681..1698
                     /label=His-tag
ORIGIN
        1 gaaattaata cgactcacta taggggaatt gtgagcggat aacaattccc ccctagaaat
       61 aattttgttt aactttaaga aggagatata catatgcacc atcatcatca tcattcttct
      121 ggtctggtgc cacgcggttc tggtatgaaa gaaaccgctg ctgctaaatt cgaacgccag
      181 cacatggaca gcccagatct gggtaccggt ggtggctccg gtattgaggg tcgcatgatg
      241 tccttttccg tactgcaagt gaaacgtctg caaccggaac tgattacacc ggcaaaatca
      301 acccctcagg aaaccaaatt tctgagcgac atcgatgatc aagaatctct gcgcgttcag
      361 attccgatta tcatgtgtta taaagacaac ccgagcctga ataaaaaccg taacccggtc
      421 aaagccattc gtgaagcact gtcacgtgcc ctggtctatt attatccact ggccggtcgt
      481 ctgcgtgaag gtccaaaccg taaactggtg gtcgattgta acggcgaagg catcctgttt
      541 gtggaagctt ccgccgatgt tacgctggaa cagctggggg acaaaatcct gcctccttgt
      601 cctctgctgg aggagtttct gtataacttc ccgggttctg atggcattat cgactgccca
      661 ctgctgctga ttcaagtgac ctgtctgact tgtggagggt tcatcctggc tctgcgcctg
      721 aaccatacaa tgtgtgatgc cgccggtctg ctgctgtttc tgaccgctat tgccgaaatg
      781 gcccgtggcg ctcatgcccc tagtattctg cctgtctggg agcgtgaact gctgtttgct
      841 cgtgatcctc ctcgtatcac ctgtgcccac catgaatatg aggacgtgat tggccactca
      901 gatgggtctt atgccagctc aaatcagagt aatatggtcc aacgctcgtt ctattttggc
      961 gccaaagaga tgcgcgtact gcgtaaacaa attccgccac acctgattag cacttgtagc
     1021 accttcgatc tgatcaccgc ttgtctgtgg aaatgtcgta ccctggcact gaacattaac
     1081 ccgaaagagg ccgttcgtgt ttcgtgtatt gtgaatgctc gtggtaaaca caacaacgtt
     1141 cgcctgcctc tgggttatta tggtaacgcc tttgcctttc cggcagcaat ttcaaaagcc
     1201 gaacctctgt gtaaaaaccc gctgggatat gctctggaac tggtaaaaaa agcgaaagcg
     1261 actatgaacg aagagtatct gcgttctgtt gctgatctgc tggtactgcg cggtcgccct
     1321 cagtatagta gtaccggctc ctatctgatc gttagcgaca acacccgtgt aggcttcgga
     1381 gatgtgaatt ttggctgggg tcagcctgtt tttgctggac cggttaaagc actggatctg
     1441 atctcgtttt atgtccaaca caaaaacaac accgaggatg gcattctggt tccgatgtgt
     1501 ctgcctagta gcgctatgga acgctttcag caggaactgg agcgtattac ccaggaacca
     1561 aaagaggaca tctgtaacaa tctgcgctca acgtcccagt aaggctctaa ctctcctctg
     1621 gccatggcga tatcggatcc aaattcgagc tccgtcgaca agcttgcggc cgcactcgag
     1681 caccaccacc accaccactg agatccggct gctaacaaag cccgaaagga agctgagttg
     1741 gctgctgcca ccgctga
//