##gff-version 3
##sequence-region BBa_K395704 1 6391
BBa_K395704	.	engineered_region	1774	6391	.	+	0	ID=BBa_K274210;Name=BBa_K274210
BBa_K395704	.	CDS	2783	3715	.	+	0	ID=BBa_K118002;Name=BBa_K118002;Parent=BBa_K274210
BBa_K395704	.	CDS	2783	3712	.	+	0	ID=annotation1995701;Name=crtB coding sequence;Parent=BBa_K118002
BBa_K395704	.	CDS	5240	6391	.	+	0	ID=BBa_K118008;Name=BBa_K118008;Parent=BBa_K274210
BBa_K395704	.	CDS	5240	6388	.	+	0	ID=annotation1995716;Name=crtY coding sequence;Parent=BBa_K118008
BBa_K395704	.	CDS	1847	2758	.	+	0	ID=BBa_I742151;Name=BBa_I742151;Parent=BBa_K274210
BBa_K395704	.	ribosome_entry_site	5230	5233	.	+	0	ID=BBa_K118012;Name=BBa_K118012;Parent=BBa_K274210
BBa_K395704	.	ribosome_entry_site	2767	2776	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274210
BBa_K395704	.	ribosome_entry_site	2770	2776	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K395704	.	sequence_feature	2767	2769	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K395704	.	ribosome_entry_site	3724	3733	.	+	0	ID=BBa_J15001;Name=BBa_J15001;Parent=BBa_K274210
BBa_K395704	.	ribosome_entry_site	3727	3733	.	+	0	ID=annotation1938046;Name=rbs;Parent=BBa_J15001
BBa_K395704	.	sequence_feature	3724	3726	.	+	0	ID=annotation1938045;Name=SacI;Parent=BBa_J15001
BBa_K395704	.	ribosome_entry_site	1837	1840	.	+	0	ID=BBa_K118012;Name=BBa_K118012;Parent=BBa_K274210
BBa_K395704	.	CDS	3740	5221	.	+	0	ID=BBa_K118003;Name=BBa_K118003;Parent=BBa_K274210
BBa_K395704	.	point_mutation	4628	4633	.	+	0	ID=annotation1968245;Name=PstI site removed;Parent=BBa_K118003
BBa_K395704	.	CDS	3740	5218	.	+	0	ID=annotation1995702;Name=crtI coding sequence;Parent=BBa_K118003
BBa_K395704	.	point_mutation	4217	4222	.	+	0	ID=annotation1968244;Name=PstI site removed;Parent=BBa_K118003
BBa_K395704	.	promoter	1774	1827	.	+	0	ID=BBa_R0011;Name=BBa_R0011;Parent=BBa_K274210
BBa_K395704	.	operator	1799	1815	.	+	0	ID=annotation2001;Name=lac O1;Parent=BBa_R0011
BBa_K395704	.	promoter	1816	1821	.	+	0	ID=annotation2002;Name=-10;Parent=BBa_R0011
BBa_K395704	.	promoter	1793	1798	.	+	0	ID=annotation2000;Name=-35;Parent=BBa_R0011
BBa_K395704	.	engineered_region	1774	1827	.	+	0	ID=annotation7064;Name=BBa_R0011;Parent=BBa_R0011
BBa_K395704	.	operator	1776	1792	.	+	0	ID=annotation1999;Name=lac O1;Parent=BBa_R0011
BBa_K395704	.	engineered_region	1	1765	.	+	0	ID=BBa_K395702;Name=BBa_K395702
BBa_K395704	.	promoter	1	1210	.	+	0	ID=BBa_I0500;Name=BBa_I0500;Parent=BBa_K395702
BBa_K395704	.	promoter	1080	1210	.	+	0	ID=annotation1498841;Name=Pbad;Parent=BBa_I0500
BBa_K395704	.	promoter	1043	1114	.	+	0	ID=annotation1498842;Name=PC;Parent=BBa_I0500
BBa_K395704	.	sequence_feature	1	1079	.	+	0	ID=annotation1498843;Name=AraC;Parent=BBa_I0500
BBa_K395704	.	engineered_region	1219	1765	.	+	0	ID=BBa_I742158;Name=BBa_I742158;Parent=BBa_K395702
BBa_K395704	.	ribosome_entry_site	1219	1234	.	+	0	ID=BBa_I742156;Name=BBa_I742156;Parent=BBa_I742158
BBa_K395704	.	CDS	1235	1765	.	+	0	ID=BBa_I742157;Name=BBa_I742157;Parent=BBa_I742158
BBa_K395704	.	CDS	1235	1762	.	+	0	ID=annotation1953812;Name=crtZ;Parent=BBa_I742157
>BBa_K395704
ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcat
tttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtgg
tgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaa
aagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtga
tcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgcc
gcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttg
cccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccag
ttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgac
cgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcac
caccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataa
ccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcg
cttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactg
cgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaa
gccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacac
tttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttc
tccatacccgtttttttgggctagctactagagctctaccggagaaattatgttgtggatttggaatgccctgatcgtt
ttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggc
atctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgat
cctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactc
tattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggt
tgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcc
cctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggag
gatgagcccgcatccgggaagtaataatactagagaattgtgagcggataacaattgacattgtgagcggataacaaga
tactgagcacatactagaggaggtactagatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcgga
gcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcg
atgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcg
ctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatat
gccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactg
gcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcggg
cggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataa
gccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcc
tcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaac
tgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaa
tctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgc
caacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactaga
gctcaaggaggtactagatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttt
tgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgac
gatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaac
ttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctat
ggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagc
caactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgc
gggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtgga
cgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggca
cctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacag
ccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaa
agttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggcc
gcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaat
aatactagagctcaaggaggtactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaat
tcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgag
gatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcag
gaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctt
taattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcag
tttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagaca
tgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaaga
tgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacg
ttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataa
agctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattga
agccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgc
gacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttg
tgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagct
gattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcg
tcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactgga
cggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagct
ggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggag
cccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcag
gcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgat
ataataatactagaggaggtactagatgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggcctt
atcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatc
atacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcc
cgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgct
gaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggt
tgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtggg
cttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacg
gtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcact
atattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagct
tcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgc
cccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtgg
ccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctg
gcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatg
cagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgta
ttctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataa