BBa_K395705
1
BBa_K395705
crtW (beta-carotene ketolase) with rbs
2010-10-05T11:00:00Z
2015-05-08T01:12:22Z
Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.
crtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.
Part of the carotenoid biosynthesis pathway. Converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin.
false
false
_505_
0
6372
9
It's complicated
true
No special considerations.
Please refer to Part BBa_K395706 for more information.
false
Yumiko Kinoshita
component2082981
1
BBa_K395700
component2082980
1
BBa_J15001
annotation2082980
1
BBa_J15001
range2082980
1
1
10
annotation2082981
1
BBa_K395700
range2082981
1
17
751
BBa_J15001
1
BBa_J15001
strong synthetic E. coli ribosome binding site with SacI site.
2007-07-12T11:00:00Z
2015-08-31T04:08:32Z
Synthetic.
This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
false
false
_163_
0
837
163
Not in stock
false
Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
false
Chris French
annotation1938046
1
rbs
range1938046
1
4
10
annotation1938045
1
SacI
range1938045
1
1
3
BBa_K395700
1
BBa_K395700
crtW (beta-carotene ketolase) coding sequence
2010-10-04T11:00:00Z
2015-05-08T01:12:22Z
Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.
Coding sequence for crtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.
Part of the carotenoid biosynthesis pathway. Converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin.
false
false
_505_
0
6372
9
Not in stock
false
No special considerations.
Please refer to Part BBa_K395706 for more information.
false
Yumiko Kinoshita
BBa_K395700_sequence
1
atgactgctgctgttgctgagcctagaatcgttcctagacagacttggatcggacttactcttgctggaatgatcgttgctggatggggatctcttcacgtttacggagtttacttccacagatggggaacttcttctcttgttatcgttcctgctatcgttgctgttcagacttggctttctgttggacttttcatcgttgctcatgatgctatgcacggatctcttgctcctggaagacctagacttaacgctgctgttggaagacttactcttggactttacgctggattcagattcgatagacttaagactgctcaccacgctcaccacgctgctcctggaactgctgatgatcctgatttctacgctcctgctcctagagctttccttccttggttccttaacttcttcagaacttacttcggatggagagagatggctgttcttactgctcttgttcttatcgctcttttcggacttggagctagacctgctaaccttcttactttctgggctgctcctgctcttctttctgctcttcagcttttcactttcggaacttggcttcctcacagacacactgatcagcctttcgctgatgctcaccacgctagatcttctggatacggacctgttctttctcttcttacttgcttccacttcggaagacaccacgagcaccaccttactccttggagaccttggtggagactttggagaggagagtcttga
BBa_J15001_sequence
1
ctcaaggagg
BBa_K395705_sequence
1
ctcaaggaggtactagatgactgctgctgttgctgagcctagaatcgttcctagacagacttggatcggacttactcttgctggaatgatcgttgctggatggggatctcttcacgtttacggagtttacttccacagatggggaacttcttctcttgttatcgttcctgctatcgttgctgttcagacttggctttctgttggacttttcatcgttgctcatgatgctatgcacggatctcttgctcctggaagacctagacttaacgctgctgttggaagacttactcttggactttacgctggattcagattcgatagacttaagactgctcaccacgctcaccacgctgctcctggaactgctgatgatcctgatttctacgctcctgctcctagagctttccttccttggttccttaacttcttcagaacttacttcggatggagagagatggctgttcttactgctcttgttcttatcgctcttttcggacttggagctagacctgctaaccttcttactttctgggctgctcctgctcttctttctgctcttcagcttttcactttcggaacttggcttcctcacagacacactgatcagcctttcgctgatgctcaccacgctagatcttctggatacggacctgttctttctcttcttacttgcttccacttcggaagacaccacgagcaccaccttactccttggagaccttggtggagactttggagaggagagtcttga
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z