BBa_I742156 1 BBa_I742156 crtZ native rbs 2007-10-23T11:00:00Z 2015-08-31T04:08:03Z Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Native ribosome binding site for crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. false false _123_ 0 837 163 Not in stock false A virtual part to be added to the coding sequence blunt (without a scar). false Chris French BBa_I742157 1 BBa_I742157 crtZ (beta-carotene hydroxylase) coding sequence 2007-10-23T11:00:00Z 2015-08-31T04:08:03Z Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Coding sequence for crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to the yellow pigment zeaxanthin. false true _123_ 0 837 163 Not in stock false No special considerations. false Chris French annotation1953812 1 crtZ range1953812 1 1 528 BBa_K395706 1 BBa_K395706 rbs+crtZW under pBad promoter for double plasmids 2010-10-07T11:00:00Z 2015-05-08T01:12:22Z CrtZ comes from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. CrtW comes from Brevundimonas sp. strain SD212 (MBIC 03018) (Accession number AB377271). . This Composite Biobrick is created by standard assembly of Part BBa_I0500, Part BBa_I742158 and Part BBa_K395705. This part contains crtZW, which works as the astaxanthin biosynthesis pathway when add beta-carotene as a substrate. It is necessary to add either beta-carotene or the synthesis operon. false false _505_ 0 6372 9 It's complicated false ・This operon is under arabinose inducible promoter. ・I used this part with beta-carotene synthesis operon (BBa_K274210) as double plasmids in the project. false Yumiko Kinoshita component2222587 1 BBa_I0500 component2222596 1 BBa_K395705 component2222591 1 BBa_I742158 annotation2222587 1 BBa_I0500 range2222587 1 1 1210 annotation2222596 1 BBa_K395705 range2222596 1 1774 2524 annotation2222591 1 BBa_I742158 range2222591 1 1219 1765 BBa_K395705 1 BBa_K395705 crtW (beta-carotene ketolase) with rbs 2010-10-05T11:00:00Z 2015-05-08T01:12:22Z Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271. crtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin. false false _505_ 0 6372 9 It's complicated true No special considerations. Please refer to Part BBa_K395706 for more information. false Yumiko Kinoshita component2082980 1 BBa_J15001 component2082981 1 BBa_K395700 annotation2082981 1 BBa_K395700 range2082981 1 17 751 annotation2082980 1 BBa_J15001 range2082980 1 1 10 BBa_I742158 1 rbs+crtZ crtZ (beta-carotene hydroxylase) with native rbs 2007-10-23T11:00:00Z 2015-08-31T04:08:03Z Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Released HQ 2013 crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to the yellow pigment zeaxnathin. false false _123_ 0 837 163 In stock true This part was made as a single biobrick rather than separate rbs and coding sequence, hence the use of blunt combination to generate the final sequence. true Chris French component1953813 1 BBa_I742156 component1953815 1 BBa_I742157 annotation1953815 1 BBa_I742157 range1953815 1 17 547 annotation1953813 1 BBa_I742156 range1953813 1 1 16 BBa_J15001 1 BBa_J15001 strong synthetic E. coli ribosome binding site with SacI site. 2007-07-12T11:00:00Z 2015-08-31T04:08:32Z Synthetic. This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector. false false _163_ 0 837 163 Not in stock false Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest. false Chris French annotation1938045 1 SacI range1938045 1 1 3 annotation1938046 1 rbs range1938046 1 4 10 BBa_K395700 1 BBa_K395700 crtW (beta-carotene ketolase) coding sequence 2010-10-04T11:00:00Z 2015-05-08T01:12:22Z Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271. Coding sequence for crtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin. false false _505_ 0 6372 9 Not in stock false No special considerations. Please refer to Part BBa_K395706 for more information. false Yumiko Kinoshita BBa_I0500 1 pBad/araC Inducible pBad/araC promoter 2003-12-05T12:00:00Z 2015-08-31T04:07:29Z -- No description -- false true _1_ 0 24 7 In stock false true Sri Kosuri annotation1498842 1 PC range1498842 1 1043 1114 annotation1498841 1 Pbad range1498841 1 1080 1210 annotation1498843 1 AraC range1498843 1 1 1079 BBa_I742157_sequence 1 atgttgtggatttggaatgccctgatcgttttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggcatctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgatcctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactctattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggttgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcccctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggaggatgagcccgcatccgggaagtaataa BBa_K395706_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagctactagagctctaccggagaaattatgttgtggatttggaatgccctgatcgttttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggcatctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgatcctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactctattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggttgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcccctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggaggatgagcccgcatccgggaagtaataatactagagctcaaggaggtactagatgactgctgctgttgctgagcctagaatcgttcctagacagacttggatcggacttactcttgctggaatgatcgttgctggatggggatctcttcacgtttacggagtttacttccacagatggggaacttcttctcttgttatcgttcctgctatcgttgctgttcagacttggctttctgttggacttttcatcgttgctcatgatgctatgcacggatctcttgctcctggaagacctagacttaacgctgctgttggaagacttactcttggactttacgctggattcagattcgatagacttaagactgctcaccacgctcaccacgctgctcctggaactgctgatgatcctgatttctacgctcctgctcctagagctttccttccttggttccttaacttcttcagaacttacttcggatggagagagatggctgttcttactgctcttgttcttatcgctcttttcggacttggagctagacctgctaaccttcttactttctgggctgctcctgctcttctttctgctcttcagcttttcactttcggaacttggcttcctcacagacacactgatcagcctttcgctgatgctcaccacgctagatcttctggatacggacctgttctttctcttcttacttgcttccacttcggaagacaccacgagcaccaccttactccttggagaccttggtggagactttggagaggagagtcttga BBa_K395700_sequence 1 atgactgctgctgttgctgagcctagaatcgttcctagacagacttggatcggacttactcttgctggaatgatcgttgctggatggggatctcttcacgtttacggagtttacttccacagatggggaacttcttctcttgttatcgttcctgctatcgttgctgttcagacttggctttctgttggacttttcatcgttgctcatgatgctatgcacggatctcttgctcctggaagacctagacttaacgctgctgttggaagacttactcttggactttacgctggattcagattcgatagacttaagactgctcaccacgctcaccacgctgctcctggaactgctgatgatcctgatttctacgctcctgctcctagagctttccttccttggttccttaacttcttcagaacttacttcggatggagagagatggctgttcttactgctcttgttcttatcgctcttttcggacttggagctagacctgctaaccttcttactttctgggctgctcctgctcttctttctgctcttcagcttttcactttcggaacttggcttcctcacagacacactgatcagcctttcgctgatgctcaccacgctagatcttctggatacggacctgttctttctcttcttacttgcttccacttcggaagacaccacgagcaccaccttactccttggagaccttggtggagactttggagaggagagtcttga BBa_J15001_sequence 1 ctcaaggagg BBa_I0500_sequence 1 ttatgacaacttgacggctacatcattcactttttcttcacaaccggcacggaactcgctcgggctggccccggtgcattttttaaatacccgcgagaaatagagttgatcgtcaaaaccaacattgcgaccgacggtggcgataggcatccgggtggtgctcaaaagcagcttcgcctggctgatacgttggtcctcgcgccagcttaagacgctaatccctaactgctggcggaaaagatgtgacagacgcgacggcgacaagcaaacatgctgtgcgacgctggcgatatcaaaattgctgtctgccaggtgatcgctgatgtactgacaagcctcgcgtacccgattatccatcggtggatggagcgactcgttaatcgcttccatgcgccgcagtaacaattgctcaagcagatttatcgccagcagctccgaatagcgcccttccccttgcccggcgttaatgatttgcccaaacaggtcgctgaaatgcggctggtgcgcttcatccgggcgaaagaaccccgtattggcaaatattgacggccagttaagccattcatgccagtaggcgcgcggacgaaagtaaacccactggtgataccattcgcgagcctccggatgacgaccgtagtgatgaatctctcctggcgggaacagcaaaatatcacccggtcggcaaacaaattctcgtccctgatttttcaccaccccctgaccgcgaatggtgagattgagaatataacctttcattcccagcggtcggtcgataaaaaaatcgagataaccgttggcctcaatcggcgttaaacccgccaccagatgggcattaaacgagtatcccggcagcaggggatcattttgcgcttcagccatacttttcatactcccgccattcagagaagaaaccaattgtccatattgcatcagacattgccgtcactgcgtcttttactggctcttctcgctaaccaaaccggtaaccccgcttattaaaagcattctgtaacaaagcgggaccaaagccatgacaaaaacgcgtaacaaaagtgtctataatcacggcagaaaagtccacattgattatttgcacggcgtcacactttgctatgccatagcatttttatccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccatacccgtttttttgggctagc BBa_K395705_sequence 1 ctcaaggaggtactagatgactgctgctgttgctgagcctagaatcgttcctagacagacttggatcggacttactcttgctggaatgatcgttgctggatggggatctcttcacgtttacggagtttacttccacagatggggaacttcttctcttgttatcgttcctgctatcgttgctgttcagacttggctttctgttggacttttcatcgttgctcatgatgctatgcacggatctcttgctcctggaagacctagacttaacgctgctgttggaagacttactcttggactttacgctggattcagattcgatagacttaagactgctcaccacgctcaccacgctgctcctggaactgctgatgatcctgatttctacgctcctgctcctagagctttccttccttggttccttaacttcttcagaacttacttcggatggagagagatggctgttcttactgctcttgttcttatcgctcttttcggacttggagctagacctgctaaccttcttactttctgggctgctcctgctcttctttctgctcttcagcttttcactttcggaacttggcttcctcacagacacactgatcagcctttcgctgatgctcaccacgctagatcttctggatacggacctgttctttctcttcttacttgcttccacttcggaagacaccacgagcaccaccttactccttggagaccttggtggagactttggagaggagagtcttga BBa_I742156_sequence 1 ctctaccggagaaatt BBa_I742158_sequence 1 ctctaccggagaaattatgttgtggatttggaatgccctgatcgttttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggcatctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgatcctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactctattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggttgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcccctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggaggatgagcccgcatccgggaagtaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z