LOCUS BBa_K395998 19024 bp DNA linear 22-Dec-2024 DEFINITION Wolf coli system ACCESSION BBa_K395998 VERSION BBa_K395998.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23107 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 788..812 /label=Help:Barcodes CDS 62..812 /label=BBa_I15008 misc_feature 825..828 /label=conserved RBS 821..832 /label=BBa_B0034 CDS 839..1588 /label=PcyA misc_feature 1589..1613 /label=Help:Barcodes CDS 839..1613 /label=BBa_I15009 stem_loop 1633..1676 /label=stem_loop misc_feature 1622..1701 /label=BBa_B0010 terminator 1622..1701 /label=BBa_B0010 stem_loop 1717..1736 /label=T7 TE misc_feature 1743..1743 /label=stop polyA_site 1737..1750 /label=polya misc_feature 1710..1750 /label=BBa_B0012 terminator 1710..1750 /label=BBa_B0012 terminator 1622..1750 /label=BBa_B0015 promoter 1759..1793 /label=BBa_J23107 misc_feature 1806..1809 /label=conserved RBS 1802..1813 /label=BBa_B0034 CDS 1820..4057 /label=Cph8 misc_feature 4058..4082 /label=Help:Barcodes CDS 1820..4082 /label=BBa_I15010 stem_loop 4102..4145 /label=stem_loop misc_feature 4091..4170 /label=BBa_B0010 terminator 4091..4170 /label=BBa_B0010 stem_loop 4186..4205 /label=T7 TE misc_feature 4212..4212 /label=stop polyA_site 4206..4219 /label=polya misc_feature 4179..4219 /label=BBa_B0012 terminator 4179..4219 /label=BBa_B0012 terminator 4091..4219 /label=BBa_B0015 misc_feature 4228..4247 /label=Binding site1 promoter 4253..4258 /label=-35 promoter 4276..4281 /label=-10 promoter 4228..4286 /label=BBa_K395303 misc_feature 4299..4302 /label=conserved RBS 4295..4306 /label=BBa_B0034 misc_feature 4313..5395 /label=BBa_K082005 CDS 4313..5395 /label=BBa_K082005 stem_loop 5415..5458 /label=stem_loop misc_feature 5404..5483 /label=BBa_B0010 terminator 5404..5483 /label=BBa_B0010 stem_loop 5499..5518 /label=T7 TE misc_feature 5525..5525 /label=stop polyA_site 5519..5532 /label=polya misc_feature 5492..5532 /label=BBa_B0012 terminator 5492..5532 /label=BBa_B0012 misc_feature 4228..5532 /label=BBa_K395407 misc_feature 5553..5570 /label=C1 OmpR misc_feature 5574..5591 /label=C2 OmpR misc_feature 5594..5611 /label=C3 OmpR promoter 5615..5620 /label=-35 promoter 5638..5643 /label=-10 promoter 5541..5648 /label=BBa_R0082 misc_feature 5661..5664 /label=conserved RBS 5657..5668 /label=BBa_B0034 CDS 5678..6385 /label=cI lambda misc_feature 6386..6418 /label=LVA misc_feature 6425..6449 /label=Help:Barcodes CDS 5675..6449 /label=BBa_C0051 stem_loop 6469..6512 /label=stem_loop misc_feature 6458..6537 /label=BBa_B0010 terminator 6458..6537 /label=BBa_B0010 stem_loop 6553..6572 /label=T7 TE misc_feature 6579..6579 /label=stop polyA_site 6573..6586 /label=polya misc_feature 6546..6586 /label=BBa_B0012 terminator 6546..6586 /label=BBa_B0012 terminator 6458..6586 /label=BBa_B0015 promoter 6609..6614 /label=-35 promoter 6619..6635 /label=OR1 promoter 6595..6611 /label=OR2 promoter 6632..6637 /label=-10 misc_feature 6595..6643 /label=BBa_R1051 promoter 6595..6643 /label=BBa_R1051 misc_feature 6656..6659 /label=conserved RBS 6652..6663 /label=BBa_B0034 CDS 6670..7755 /label=BBa_I732100 stem_loop 7775..7818 /label=stem_loop misc_feature 7764..7843 /label=BBa_B0010 terminator 7764..7843 /label=BBa_B0010 stem_loop 7859..7878 /label=T7 TE misc_feature 7885..7885 /label=stop polyA_site 7879..7892 /label=polya misc_feature 7852..7892 /label=BBa_B0012 terminator 7852..7892 /label=BBa_B0012 terminator 7764..7892 /label=BBa_B0015 misc_feature 7903..7919 /label=lac O1 promoter 7920..7925 /label=-35 misc_feature 7926..7942 /label=lac O1 promoter 7943..7948 /label=-10 misc_feature 7901..7954 /label=BBa_R0011 promoter 7901..7954 /label=BBa_R0011 misc_feature 7968..7971 /label=conserved RBS 7964..7975 /label=BBa_B0034 CDS 7985..8601 /label=tetR misc_feature 8602..8635 /label=SsrA misc_feature 8642..8666 /label=Help:Barcodes CDS 7982..8641 /label=BBa_C0040 stem_loop 8686..8729 /label=stem_loop misc_feature 8675..8754 /label=BBa_B0010 terminator 8675..8754 /label=BBa_B0010 stem_loop 8770..8789 /label=T7 TE misc_feature 8796..8796 /label=stop polyA_site 8790..8803 /label=polya misc_feature 8763..8803 /label=BBa_B0012 terminator 8763..8803 /label=BBa_B0012 misc_feature 8812..8830 /label=TetR 1 misc_feature 8812..8865 /label=BBa_R0040 promoter 8831..8836 /label=-35 misc_feature 8837..8855 /label=TetR 2 promoter 8854..8859 /label=-10 promoter 8812..8865 /label=BBa_R0040 misc_feature 7964..8865 /label=BBa_Q04400 misc_feature 8878..8881 /label=conserved RBS 8874..8885 /label=BBa_B0034 misc_feature 8892..8893 /label=prefix misc_feature 9065..9065 /label=T misc_feature 9383..9383 /label=A CDS 8892..9641 /label=luxR misc_feature 8892..9647 /label=BBa_C0062 misc_feature 9648..9672 /label=Help:Barcodes CDS 8892..9647 /label=BBa_C0062 stem_loop 9692..9735 /label=stem_loop misc_feature 9681..9760 /label=BBa_B0010 terminator 9681..9760 /label=BBa_B0010 stem_loop 9776..9795 /label=T7 TE misc_feature 9802..9802 /label=stop polyA_site 9796..9809 /label=polya misc_feature 9769..9809 /label=BBa_B0012 terminator 9769..9809 /label=BBa_B0012 misc_feature 8874..9809 /label=BBa_I0462 promoter 9818..9823 /label=-35 promoter 9842..9847 /label=-10 misc_feature 9823..9842 /label=Lux Box promoter 9818..9847 /label=BBa_R0061 RBS 9856..9870 /label=RBS-1\Strong RBS 9863..9867 /label=RBS misc_feature 9856..9870 /label=BBa_B0030 RBS 9856..9870 /label=BBa_B0030 CDS 9877..10479 /label=LasI misc_feature 10480..10512 /label=LVA misc_feature 10519..10543 /label=Help:Barcodes CDS 9877..10543 /label=BBa_C0078 misc_feature 10552..10590 /label=B1006 stem_loop 10561..10582 /label=modified thr terminator polyA_site 10583..10590 /label=PolyA polyA_site 10552..10560 /label=PolyA terminator 10552..10590 /label=BBa_B1006 misc_feature 9856..10590 /label=BBa_K081016 misc_feature 10599..10618 /label=LuxR/HSL promoter 10618..10623 /label=-35 promoter 10640..10645 /label=-10 misc_feature 10651..10651 /label=start misc_feature 10599..10653 /label=BBa_R0062 promoter 10599..10653 /label=BBa_R0062 RBS 10667..10670 /label=RBS misc_feature 10678..11338 /label=CmR misc_feature 10662..11340 /label=BBa_K395160 misc_feature 10599..11340 /label=BBa_K395162 RBS 11349..11352 /label=BBa_K118012 CDS 11359..12270 /label=BBa_I742151 misc_feature 12279..12281 /label=SacI RBS 12282..12288 /label=rbs RBS 12279..12288 /label=BBa_J15001 CDS 12295..13224 /label=crtB coding sequence CDS 12295..13227 /label=BBa_K118002 misc_feature 13236..13238 /label=SacI RBS 13239..13245 /label=rbs RBS 13236..13245 /label=BBa_J15001 misc_feature 13729..13734 /label=PstI site removed misc_feature 14140..14145 /label=PstI site removed CDS 13252..14730 /label=crtI coding sequence CDS 13252..14733 /label=BBa_K118003 RBS 14742..14745 /label=BBa_K118012 CDS 14752..15900 /label=crtY coding sequence CDS 14752..15903 /label=BBa_K118008 misc_feature 11349..15903 /label=BBa_K274200 RBS 15912..15927 /label=BBa_I742156 CDS 15928..16455 /label=crtZ CDS 15928..16458 /label=BBa_I742157 misc_feature 15912..16458 /label=BBa_I742158 misc_feature 16467..16469 /label=SacI RBS 16470..16476 /label=rbs RBS 16467..16476 /label=BBa_J15001 CDS 16483..17217 /label=BBa_K395700 misc_feature 16467..17217 /label=BBa_K395705 RBS 17235..17239 /label=rbs misc_feature 17249..17249 /label=start misc_feature 17252..17269 /label=his-tag misc_feature 17303..17336 /label=s-tag misc_feature 17378..17389 /label=factor-Xa CDS 17390..18757 /label=MpAAT1 misc_feature 18757..18757 /label=stop misc_feature 18836..18853 /label=His-tag misc_feature 17226..18912 /label=BBa_K395604 terminator 18921..18968 /label=BBa_K395601 misc_feature 17226..18968 /label=BBa_K395603 terminator 18977..19024 /label=BBa_K395601 ORIGIN 1 tttacggcta gctcagccct aggtattatg ctagctacta gagaaagagg agaaatacta 61 gatgagtgtc aacttagctt cccagttgcg ggaagggacg aaaaaatccc actccatggc 121 ggagaacgtc ggctttgtca aatgcttcct caagggcgtt gtcgagaaaa attcctaccg 181 taagctggtt ggcaatctct actttgtcta cagtgccatg gaagaggaaa tggcaaaatt 241 taaggaccat cccatcctca gccacattta cttccccgaa ctcaaccgca aacaaagcct 301 agagcaagac ctgcaattct attacggctc caactggcgg caagaagtga aaatttctgc 361 cgctggccaa gcctatgtgg accgagtccg gcaagtggcc gctacggccc ctgaattgtt 421 ggtggcccat tcctacaccc gttacctggg ggatctttcc ggcggtcaaa ttctcaagaa 481 aattgcccaa aatgccatga atctccacga tggtggcaca gctttctatg aatttgccga 541 cattgatgac gaaaaggctt ttaaaaatac ctaccgtcaa gctatgaatg atctgcccat 601 tgaccaagcc accgccgaac ggattgtgga tgaagccaat gacgcctttg ccatgaacat 661 gaaaatgttc aacgaacttg aaggcaacct gatcaaggcg atcggcatta tggtgttcaa 721 cagcctcacc cgtcgccgca gtcaaggcag caccgaagtt ggcctcgcca cctccgaagg 781 ctaataacgc tgatagtgct agtgtagatc gctactagag aaagaggaga aatactagat 841 ggccgtcact gatttaagtt tgaccaattc ttccctgatg cctacgttga acccgatgat 901 tcaacagttg gccctggcga tcgccgctag ttggcaaagt ttacccctca agccctatca 961 attgccggag gatttgggct acgtagaagg ccgcctggaa ggggaaaagt tagtgattga 1021 aaatcggtgc taccaaacgc cccagtttcg caaaatgcat ttggagttgg ccaaggtggg 1081 caaagggttg gatattctcc actgtgtaat gtttcctgag cctttatacg gtctaccttt 1141 gtttggctgt gacattgtgg ccggccccgg tggagtaagt gcggctattg cggatctatc 1201 ccccacccaa agcgatcgcc aattgcccgc agcgtaccaa aaatcattgg cagagctagg 1261 ccagccagaa tttgagcaac aacgggaatt gcccccctgg ggagaaatat tttctgaata 1321 ttgtttattc atccgtccca gcaatgtcac tgaagaagaa agatttgtac aaagggtagt 1381 ggactttttg caaattcatt gtcaccaatc catcgttgcc gaacccttgt ctgaagctca 1441 aactttggag caccgtcagg ggcaaattca ttactgccaa caacaacaga aaaatgataa 1501 aacccgtcgg gtactggaaa aagcttttgg ggaagcttgg gcggaacggt atatgagcca 1561 agtcttattt gatgttatcc aataataact ctgatagtgc tagtgtagat ctctactaga 1621 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 1681 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 1741 tgcgtttata tactagagtt tacggctagc tcagccctag gtattatgct agctactaga 1801 gaaagaggag aaatactaga tggccaccac cgtacaactc agcgaccaat ccctccgtca 1861 gctagaaacc ctcgccatcc acaccgccca cctgattcag ccccacggtt tagtggtggt 1921 cctgcaagaa ccagacctca ccatcagcca aattagcgcc aactgcaccg gcattttagg 1981 gcgatcgcca gaggatttgt tgggcagaac cctaggggaa gtgtttgata gctttcagat 2041 tgatcccatc cagagtcgcc taacggccgg acaaatcagc agcctcaacc ccagtaaact 2101 ttgggcgcgg gtcatggggg acgactttgt catttttgac ggggtttttc atcgcaacag 2161 tgacggttta ttggtatgtg aactcgagcc agcctacact tccgataatc tgcccttcct 2221 cggtttttat cacatggcca acgctgccct gaatcggttg cgccaacaag ctaatctacg 2281 ggatttctac gatgttattg tcgaagaagt ccgccgtatg actggctttg accgggtgat 2341 gctataccgc tttgatgaaa ataaccacgg tgatgtcatt gccgaagata aacgggatga 2401 tatggaaccc tatttgggcc tgcactatcc cgaatcggat attccccaac ccgcccgtcg 2461 gctatttatc cacaacccca ttcgagtaat tcccgatgtt tatggtgtgg cggtgcccct 2521 gaccccagcg gttaacccca gcaccaaccg agcggtggat ttaacagaat ccattctgcg 2581 cagtgcgtac cattgccact tgacctatct gaaaaatatg ggggtaggag cgtctttaac 2641 catttcccta attaaggacg gccatctctg ggggctcatt gcctgccacc atcaaacccc 2701 caaagtaatt ccctttgaac tgcgtaaagc ctgcgaattt tttggtcggg tggtgtttag 2761 caacatttcc gcccaggaag atacggaaac cttcgattac cgggtgcagc tggcggagca 2821 tgaagcggtt ttattggaca aaatgaccac ggcggcggat tttgtcgaag gattaactaa 2881 tcatcccgat cgcctgttgg gattaacggg ctcccagggg gcggccattt gctttgggga 2941 aaaattgatt ttagtagggg aaaccccgga cgagaaagca gtgcaatatt tactgcaatg 3001 gttggagaat cgggaagtgc aagacgtttt cttcacctct tccctctcac aaatttatcc 3061 tgatgcagtg aattttaaat ccgtggccag tggcttattg gccattccca ttgcccgtca 3121 caactttttg ctctggtttc gccctgaagt gttgcaaacg gttaattggg gcggtgaccc 3181 aaatcatgct tacgaagcta cccaggaaga cggtaaaatc gagctccatc cccgccaatc 3241 ctttgacctc tggaaagaaa ttgtccgact ccaatctttg ccctggcaat cggtggaaat 3301 ccaaagtgcc ctggccctga aaaaggcgat cgtcaacctc attttgcgcc aggcagaaga 3361 attgcatatg gcggctggtg ttaagcaact ggcggatgac cgcacgctgc tgatggcggg 3421 ggtaagtcac gacttgcgca cgccgctgac gcgtattcgc ctggcgactg agatgatgag 3481 cgagcaggat ggctatctgg cagaatcgat caataaagat atcgaagagt gcaacgccat 3541 cattgagcag tttatcgact acctgcgcac cgggcaggag atgccgatgg aaatggcgga 3601 tcttaatgca gtactcggtg aggtgattgc tgccgaaagt ggctatgagc gggaaattga 3661 aaccgcgctt taccccggca gcattgaagt gaaaatgcac ccgctgtcga tcaaacgcgc 3721 ggtggcgaat atggtggtca acgccgcccg ttatggcaat ggctgggtca aagtcagcag 3781 cggaacggag ccgaatcgcg cctggttcca ggtggaagat gacggtccgg gaattgcgcc 3841 ggaacaacgt aagcacctgt tccagccgtt tgtccgcggc gacagtgcgc gcaccattag 3901 cggcacggga ttagggctgg caattgtgca gcgtatcgtg gataaccata acgggatgct 3961 ggagcttggc accagcgagc ggggcgggct ttccattcgc gcctggctgc cagtgccggt 4021 aacgcgggcg cagggcatga caaaagaagg gtaataactc tgatagtgct agtgtagatc 4081 tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 4141 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 4201 gggcctttct gcgtttatat actagagatt tacattttga aacatctgac cgttggatta 4261 ttctgcattt ttggggagaa tggacttact agagaaagag gagaaatact agatgaagcc 4321 ggtgaccctg tatgacgtgg cggaatacgc gggcgtgagc taccaaacgg tgagccgtgt 4381 tgttaatcaa gcgtctcatg tgagcgccaa gacccgcgag aaggttgagg ccgccatggc 4441 cgaattaaac tatatcccga atcgtgttgc gcagcagtta gccggtaagc aaagcctgtt 4501 aatcggtgtg gcgacgagca gcttagcgtc acatgcccca agccagatcg tggccgccat 4561 caagagccgt gcggaccagt taggcgcgtc tgttgttgtt agaatggtgg agcgctctgg 4621 tgtggaggcg tgcaaggccg ccgttcataa cctgctggcc cagcgtgtga gcggcttaat 4681 tatcaattac ccattagacg atcaagacgc gatcgcggtt gaggcggcgt gtaccaacgt 4741 gccagccctg ttcctggacg tgagcgatca aaccccgatt aatagcatca tctttagcca 4801 cgagaatggc acccgcttag gtgttgaaca cttagtggcc ctgggccatc aacagattgc 4861 cctactggcc ggtccgctga gcagcgtgag cgcccgctta cgcttagcgg gttggcacaa 4921 gtacctgacc cgtaaccaga tccaaccaat tgccgagcgc gagggtgatt ggagcgcgat 4981 gagcggcttc cagcagacga tgcagatgtt aaacgaaggt attgtgccga ccgccatgtt 5041 agtggcgaat gaccaaatgg ccttaggtgc gatgcgtgcg atcacggaaa gcggcttacg 5101 tgtgggcgcc gacattagcg tggttggcta tgatgacacg gaggattcta gctgctacat 5161 tccaccaagc acgacgatta agcaagactt ccgtttatta ggccagacgt ctgttgatcg 5221 tctgttacag ctgagccaag gtcaagccgt taaaggtaac caattactgc cggtgagctt 5281 agttaagcgc aagacgacgt tagccccgaa cacccagacg gcgagcccgc gtgccctggc 5341 ggacagcctg atgcaattag cgcgccaagt gagccgctta gagtctggcc aatgatacta 5401 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 5461 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 5521 tctgcgttta tatactagag tcccttgcat ttacattttg aaacatctat agcgataaat 5581 gaaacatctt aaaagtttta gtatcatatt cgtgttggat tattctgcat ttttggggag 5641 aatggactta ctagagaaag aggagaaata ctagatgagc acaaaaaaga aaccattaac 5701 acaagagcag cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga 5761 acttggctta tcccaggaat ctgtcgcaga caagatgggg atggggcagt caggcgttgg 5821 tgctttattt aatggcatca atgcattaaa tgcttataac gccgcattgc ttgcaaaaat 5881 tctcaaagtt agcgttgaag aatttagccc ttcaatcgcc agagaaatct acgagatgta 5941 tgaagcggtt agtatgcagc cgtcacttag aagtgagtat gagtaccctg ttttttctca 6001 tgttcaggca gggatgttct cacctgagct tagaaccttt accaaaggtg atgcggagag 6061 atgggtaagc acaaccaaaa aagccagtga ttctgcattc tggcttgagg ttgaaggtaa 6121 ttccatgacc gcaccaacag gctccaagcc aagctttcct gacggaatgt taattctcgt 6181 tgaccctgag caggctgttg agccaggtga tttctgcata gccagacttg ggggtgatga 6241 gtttaccttc aagaaactga tcagggatag cggtcaggtg tttttacaac cactaaaccc 6301 acagtaccca atgatcccat gcaatgagag ttgttccgtt gtggggaaag ttatcgctag 6361 tcagtggcct gaagagacgt ttggcgctgc aaacgacgaa aactacgctt tagtagctta 6421 ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag 6481 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 6541 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtaacac 6601 cgtgcgtgtt gacaatttta cctctggcgg tgatactggt tgctactaga gaaagaggag 6661 aaatactaga tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 6721 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 6781 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 6841 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 6901 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 6961 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 7021 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 7081 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 7141 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 7201 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 7261 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 7321 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 7381 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 7441 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa 7501 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 7561 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 7621 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 7681 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 7741 agcgggcagt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 7801 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 7861 ctcaccttcg ggtgggcctt tctgcgttta tatactagag aattgtgagc ggataacaat 7921 tgacattgtg agcggataac aagatactga gcacatacta gagaaagagg agaaatacta 7981 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 8041 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 8101 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 8161 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 8221 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 8281 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 8341 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 8401 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 8461 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 8521 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 8581 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 8641 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 8701 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 8761 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca 8821 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg 8881 agaaatacta gatgaaaaac ataaatgccg acgacacata cagaataatt aataaaatta 8941 aagcttgtag aagcaataat gatattaatc aatgcttatc tgatatgact aaaatggtac 9001 attgtgaata ttatttactc gcgatcattt atcctcattc tatggttaaa tctgatattt 9061 caatcctaga taattaccct aaaaaatgga ggcaatatta tgatgacgct aatttaataa 9121 aatatgatcc tatagtagat tattctaact ccaatcattc accaattaat tggaatatat 9181 ttgaaaacaa tgctgtaaat aaaaaatctc caaatgtaat taaagaagcg aaaacatcag 9241 gtcttatcac tgggtttagt ttccctattc atacggctaa caatggcttc ggaatgctta 9301 gttttgcaca ttcagaaaaa gacaactata tagatagttt atttttacat gcgtgtatga 9361 acataccatt aattgttcct tctctagttg ataattatcg aaaaataaat atagcaaata 9421 ataaatcaaa caacgattta accaaaagag aaaaagaatg tttagcgtgg gcatgcgaag 9481 gaaaaagctc ttgggatatt tcaaaaatat taggttgcag tgagcgtact gtcactttcc 9541 atttaaccaa tgcgcaaatg aaactcaata caacaaaccg ctgccaaagt atttctaaag 9601 caattttaac aggagcaatt gattgcccat actttaaaaa ttaataacac tgatagtgct 9661 agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 9721 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 9781 accttcgggt gggcctttct gcgtttatat actagagttg acacctgtag gatcgtacag 9841 gtataattac tagagattaa agaggagaaa tactagatga tcgttcagat cggtcgtcgt 9901 gaagagttcg acaaaaaact gctgggtgaa atgcacaaac tgcgtgctca ggttttcaaa 9961 gaacgtaaag gttgggacgt ttccgttatc gacgaaatgg aaatcgacgg ttacgacgct 10021 ctgtccccgt actacatgct gatccaggaa gacaccccgg aagctcaggt tttcggttgc 10081 tggcgtatct tcgacaccac cggtccgtac atgctgaaaa acaccttccc ggaactgctg 10141 cacggtaaag aagctccgtg ctccccgcac atctgggaac tgtcccgttt cgctatcaac 10201 tccggtcaga aaggttccct gggtttctcc gactgcaccc tggaagctat gcgtgctctg 10261 gctcgttact ccttgcagaa cgacatccag accctggtta ccgttaccac cgttggtgtt 10321 gaaaaaatga tgatccgtgc tggtctggac gtttcccgtt tcggtccgca cctgaaaatc 10381 ggtatcgaac gtgctgttgc tctgcgtatc gaactgaacg ctaaaaccca gatcgctctg 10441 tacggtggtg ttctggttga acagcgtctg gctgtttccg ctgctaacga cgaaaactac 10501 gctctggttg cttaataact ctgatagtgc tagtgtagat ctctactaga gaaaaaaaaa 10561 ccccgcccct gacagggcgg ggtttttttt tactagagac ctgtaggatc gtacaggttt 10621 acgcaagaaa atggtttgtt atagtcgaat aaatactaga gagctaagga agctaaaatg 10681 gagaaaaaaa tcacgggata taccaccgtt gatatatccc aatggcatcg taaagaacat 10741 tttgaggcat ttcagtcagt tgctcaatgt acctataacc agaccgttca gctggatatt 10801 acggcctttt taaagaccgt aaagaaaaat aagcacaagt tttatccggc ctttattcac 10861 attcttgccc gcctgatgaa cgctcacccg gagtttcgta tggccatgaa agacggtgag 10921 ctggtgatct gggatagtgt tcacccttgt tacaccgttt tccatgagca aactgaaacg 10981 ttttcgtccc tctggagtga ataccacgac gatttccggc agtttctcca catatattcg 11041 caagatgtgg cgtgttacgg tgaaaacctg gcctatttcc ctaaagggtt tattgagaat 11101 atgttttttg tctcagccaa tccctgggtg agtttcacca gttttgattt aaacgtggcc 11161 aatatggaca acttcttcgc ccccgttttc acgatgggca aatattatac gcaaggcgac 11221 aaggtgctga tgccgctggc gatccaggtt catcatgccg tttgtgatgg cttccatgtc 11281 ggccgcatgc ttaatgaatt acaacagtac tgtgatgagt ggcagggcgg ggcgtaataa 11341 tactagagga ggtactagat gacggtctgc gcaaaaaaac acgttcatct cactcgcgat 11401 gctgcggagc agttactggc tgatattgat cgacgccttg atcagttatt gcccgtggag 11461 ggagaacggg atgttgtggg tgccgcgatg cgtgaaggtg cgctggcacc gggaaaacgt 11521 attcgcccca tgttgctgtt gctgaccgcc cgcgatctgg gttgcgctgt cagccatgac 11581 ggattactgg atttggcctg tgcggtggaa atggtccacg cggcttcgct gatccttgac 11641 gatatgccct gcatggacga tgcgaagctg cggcgcggac gccctaccat tcattctcat 11701 tacggagagc atgtggcaat actggcggcg gttgccttgc tgagtaaagc ctttggcgta 11761 attgccgatg cagatggcct cacgccgctg gcaaaaaatc gggcggtttc tgaactgtca 11821 aacgccatcg gcatgcaagg attggttcag ggtcagttca aggatctgtc tgaaggggat 11881 aagccgcgca gcgctgaagc tattttgatg acgaatcact ttaaaaccag cacgctgttt 11941 tgtgcctcca tgcagatggc ctcgattgtt gcgaatgcct ccagcgaagc gcgtgattgc 12001 ctgcatcgtt tttcacttga tcttggtcag gcatttcaac tgctggacga tttgaccgat 12061 ggcatgaccg acaccggtaa ggatagcaat caggacgccg gtaaatcgac gctggtcaat 12121 ctgttaggcc cgagggcggt tgaagaacgt ctgagacaac atcttcagct tgccagtgag 12181 catctctctg cggcctgcca acacgggcac gccactcaac attttattca ggcctggttt 12241 gacaaaaaac tcgctgccgt cagttaataa tactagagct caaggaggta ctagatgaat 12301 aatccgtcgt tactcaatca tgcggtcgaa acgatggcag ttggctcgaa aagttttgcg 12361 acagcctcaa agttatttga tgcaaaaacc cggcgcagcg tactgatgct ctacgcctgg 12421 tgccgccatt gtgacgatgt tattgacgat cagacgctgg gctttcaggc ccggcagcct 12481 gccttacaaa cgcccgaaca acgtctgatg caacttgaga tgaaaacgcg ccaggcctat 12541 gcaggatcgc agatgcacga accggcgttt gcggcttttc aggaagtggc tatggctcat 12601 gatatcgccc cggcttacgc gtttgatcat ctggaaggct tcgccatgga tgtacgcgaa 12661 gcgcaataca gccaactgga tgatacgctg cgctattgct atcacgttgc aggcgttgtc 12721 ggcttgatga tggcgcaaat catgggcgtg cgggataacg ccacgctgga ccgcgcctgt 12781 gaccttgggc tggcatttca gttgaccaat attgctcgcg atattgtgga cgatgcgcat 12841 gcgggccgct gttatctgcc ggcaagctgg ctggagcatg aaggtctgaa caaagagaat 12901 tatgcggcac ctgaaaaccg tcaggcgctg agccgtatcg cccgtcgttt ggtgcaggaa 12961 gcagaacctt actatttgtc tgccacagcc ggcctggcag ggttgcccct gcgttccgcc 13021 tgggcaatcg ctacggcgaa gcaggtttac cggaaaatag gtgtcaaagt tgaacaggcc 13081 ggtcagcaag cctgggatca gcggcagtca acgaccacgc ccgaaaaatt aacgctgctg 13141 ctggccgcct ctggtcaggc ccttacttcc cggatgcggg ctcatcctcc ccgccctgcg 13201 catctctggc agcgcccgct ctaataatac tagagctcaa ggaggtacta gatgaaacca 13261 actacggtaa ttggtgcagg cttcggtggc ctggcactgg caattcgtct acaagctgcg 13321 gggatccccg tcttactgct tgaacaacgt gataaacccg gcggtcgggc ttatgtctac 13381 gaggatcagg ggtttacctt tgatgcaggc ccgacggtta tcaccgatcc cagtgccatt 13441 gaagaactgt ttgcactggc aggaaaacag ttaaaagagt atgtcgaact gctgccggtt 13501 acgccgtttt accgcctgtg ttgggagtca gggaaggtct ttaattacga taacgatcaa 13561 acccggctcg aagcgcagat tcagcagttt aatccccgcg atgtcgaagg ttatcgtcag 13621 tttctggact attcacgcgc ggtgtttaaa gaaggctatc taaagctcgg tactgtccct 13681 tttttatcgt tcagagacat gcttcgcgcc gcacctcaac tggcgaaact gcaagcatgg 13741 agaagcgttt acagtaaggt tgccagttac atcgaagatg aacatctgcg ccaggcgttt 13801 tctttccact cgctgttggt gggcggcaat cccttcgcca cctcatccat ttatacgttg 13861 atacacgcgc tggagcgtga gtggggcgtc tggtttccgc gtggcggcac cggcgcatta 13921 gttcagggga tgataaagct gtttcaggat ctgggtggcg aagtcgtgtt aaacgccaga 13981 gtcagccata tggaaacgac aggaaacaag attgaagccg tgcatttaga ggacggtcgc 14041 aggttcctga cgcaagccgt cgcgtcaaat gcagatgtgg ttcataccta tcgcgacctg 14101 ttaagccagc accctgccgc ggttaagcag tccaacaaac tgcaaactaa gcgcatgagt 14161 aactctctgt ttgtgctcta ttttggtttg aatcaccatc atgatcagct cgcgcatcac 14221 acggtttgtt tcggcccgcg ttaccgcgag ctgattgacg aaatttttaa tcatgatggc 14281 ctcgcagagg acttctcact ttatctgcac gcgccctgtg tcacggattc gtcactggcg 14341 cctgaaggtt gcggcagtta ctatgtgttg gcgccggtgc cgcatttagg caccgcgaac 14401 ctcgactgga cggttgaggg gccaaaacta cgcgaccgta tttttgcgta ccttgagcag 14461 cattacatgc ctggcttacg gagtcagctg gtcacgcacc ggatgtttac gccgtttgat 14521 tttcgcgacc agcttaatgc ctatcatggc tcagcctttt ctgtggagcc cgttcttacc 14581 cagagcgcct ggtttcggcc gcataaccgc gataaaacca ttactaatct ctacctggtc 14641 ggcgcaggca cgcatcccgg cgcaggcatt cctggcgtca tcggctcggc aaaagcgaca 14701 gcaggtttga tgctggagga tctgatataa taatactaga ggaggtacta gatgcaaccg 14761 cattatgatc tgattctcgt gggggctgga ctcgcgaatg gccttatcgc cctgcgtctt 14821 cagcagcagc aacctgatat gcgtattttg cttatcgacg ccgcacccca ggcgggcggg 14881 aatcatacgt ggtcatttca ccacgatgat ttgactgaga gccaacatcg ttggatagct 14941 ccgctggtgg ttcatcactg gcccgactat caggtacgct ttcccacacg ccgtcgtaag 15001 ctgaacagcg gctacttttg tattacttct cagcgtttcg ctgaggtttt acagcgacag 15061 tttggcccgc acttgtggat ggataccgcg gtcgcagagg ttaatgcgga atctgttcgg 15121 ttgaaaaagg gtcaggttat cggtgcccgc gcggtgattg acgggcgggg ttatgcggca 15181 aattcagcac tgagcgtggg cttccaggcg tttattggcc aggaatggcg attgagccac 15241 ccgcatggtt tatcgtctcc cattatcatg gatgccacgg tcgatcagca aaatggttat 15301 cgcttcgtgt acagcctgcc gctctcgccg accagattgt taattgaaga cacgcactat 15361 attgataatg cgacattaga tcctgaatgc gcgcggcaaa atatttgcga ctatgccgcg 15421 caacagggtt ggcagcttca gacactgctg cgagaagaac agggcgcctt acccattact 15481 ctgtcgggca atgccgacgc attctggcag cagcgccccc tggcctgtag tggattacgt 15541 gccggtctgt tccatcctac caccggctat tcactgccgc tggcggttgc cgtggccgac 15601 cgcctgagtg cacttgatgt ctttacgtcg gcctcaattc accatgccat tacgcatttt 15661 gcccgcgagc gctggcagca gcagggcttt ttccgcatgc tgaatcgcat gctgttttta 15721 gccggacccg ccgattcacg ctggcgggtt atgcagcgtt tttatggttt acctgaagat 15781 ttaattgccc gtttttatgc gggaaaactc acgctgaccg atcggctacg tattctgagc 15841 ggcaagccgc ctgttccggt attagcagca ttgcaagcca ttatgacgac tcatcgttaa 15901 taatactaga gctctaccgg agaaattatg ttgtggattt ggaatgccct gatcgttttc 15961 gttaccgtga ttggcatgga agtgattgct gcactggcac acaaatacat catgcacggc 16021 tggggttggg gatggcatct ttcacatcat gaaccgcgta aaggtgcgtt tgaagttaac 16081 gatctttatg ccgtggtttt tgctgcatta tcgatcctgc tgatttatct gggcagtaca 16141 ggaatgtggc cgctccagtg gattggcgca ggtatgacgg cgtatggatt actctatttt 16201 atggtgcacg acgggctggt gcatcaacgt tggccattcc gctatattcc acgcaagggc 16261 tacctcaaac ggttgtatat ggcgcaccgt atgcatcacg ccgtcagggg caaagaaggt 16321 tgtgtttctt ttggcttcct ctatgcgccg cccctgtcaa aacttcaggc gacgctccgg 16381 gaaagacatg gcgctagagc gggcgctgcc agagatgcgc agggcgggga ggatgagccc 16441 gcatccggga agtaataata ctagagctca aggaggtact agatgactgc tgctgttgct 16501 gagcctagaa tcgttcctag acagacttgg atcggactta ctcttgctgg aatgatcgtt 16561 gctggatggg gatctcttca cgtttacgga gtttacttcc acagatgggg aacttcttct 16621 cttgttatcg ttcctgctat cgttgctgtt cagacttggc tttctgttgg acttttcatc 16681 gttgctcatg atgctatgca cggatctctt gctcctggaa gacctagact taacgctgct 16741 gttggaagac ttactcttgg actttacgct ggattcagat tcgatagact taagactgct 16801 caccacgctc accacgctgc tcctggaact gctgatgatc ctgatttcta cgctcctgct 16861 cctagagctt tccttccttg gttccttaac ttcttcagaa cttacttcgg atggagagag 16921 atggctgttc ttactgctct tgttcttatc gctcttttcg gacttggagc tagacctgct 16981 aaccttctta ctttctgggc tgctcctgct cttctttctg ctcttcagct tttcactttc 17041 ggaacttggc ttcctcacag acacactgat cagcctttcg ctgatgctca ccacgctaga 17101 tcttctggat acggacctgt tctttctctt cttacttgct tccacttcgg aagacaccac 17161 gagcaccacc ttactccttg gagaccttgg tggagacttt ggagaggaga gtcttgatac 17221 tagagaactt taagaaggag atatacatat gcaccatcat catcatcatt cttctggtct 17281 ggtgccacgc ggttctggta tgaaagaaac cgctgctgct aaattcgaac gccagcacat 17341 ggacagccca gatctgggta ccggtggtgg ctccggtatt gagggtcgca tgatgtcctt 17401 ttccgtactg caagtgaaac gtctgcaacc ggaactgatt acaccggcaa aatcaacccc 17461 tcaggaaacc aaatttctga gcgacatcga tgatcaagaa tctctgcgcg ttcagattcc 17521 gattatcatg tgttataaag acaacccgag cctgaataaa aaccgtaacc cggtcaaagc 17581 cattcgtgaa gcactgtcac gtgccctggt ctattattat ccactggccg gtcgtctgcg 17641 tgaaggtcca aaccgtaaac tggtggtcga ttgtaacggc gaaggcatcc tgtttgtgga 17701 agcttccgcc gatgttacgc tggaacagct gggggacaaa atcctgcctc cttgtcctct 17761 gctggaggag tttctgtata acttcccggg ttctgatggc attatcgact gcccactgct 17821 gctgattcaa gtgacctgtc tgacttgtgg agggttcatc ctggctctgc gcctgaacca 17881 tacaatgtgt gatgccgccg gtctgctgct gtttctgacc gctattgccg aaatggcccg 17941 tggcgctcat gcccctagta ttctgcctgt ctgggagcgt gaactgctgt ttgctcgtga 18001 tcctcctcgt atcacctgtg cccaccatga atatgaggac gtgattggcc actcagatgg 18061 gtcttatgcc agctcaaatc agagtaatat ggtccaacgc tcgttctatt ttggcgccaa 18121 agagatgcgc gtactgcgta aacaaattcc gccacacctg attagcactt gtagcacctt 18181 cgatctgatc accgcttgtc tgtggaaatg tcgtaccctg gcactgaaca ttaacccgaa 18241 agaggccgtt cgtgtttcgt gtattgtgaa tgctcgtggt aaacacaaca acgttcgcct 18301 gcctctgggt tattatggta acgcctttgc ctttccggca gcaatttcaa aagccgaacc 18361 tctgtgtaaa aacccgctgg gatatgctct ggaactggta aaaaaagcga aagcgactat 18421 gaacgaagag tatctgcgtt ctgttgctga tctgctggta ctgcgcggtc gccctcagta 18481 tagtagtacc ggctcctatc tgatcgttag cgacaacacc cgtgtaggct tcggagatgt 18541 gaattttggc tggggtcagc ctgtttttgc tggaccggtt aaagcactgg atctgatctc 18601 gttttatgtc caacacaaaa acaacaccga ggatggcatt ctggttccga tgtgtctgcc 18661 tagtagcgct atggaacgct ttcagcagga actggagcgt attacccagg aaccaaaaga 18721 ggacatctgt aacaatctgc gctcaacgtc ccagtaaggc tctaactctc ctctggccat 18781 ggcgatatcg gatccaaatt cgagctccgt cgacaagctt gcggccgcac tcgagcacca 18841 ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg agttggctgc 18901 tgccaccgct gatactagag ctagcataac cccttggggc ctctaaacgg gtcttgaggg 18961 gttttttgta ctagagctag cataacccct tggggcctct aaacgggtct tgaggggttt 19021 tttg //