BBa_K395998
type: ComponentDefinition
persistent identity: BBa K395998
display id: BBa_K395998
version: 1
was generated by: iGEM to SBOL conversion
description: Wolf coli system
created: 2010-10-18T11:00:00Z
modified: 2015-05-08T01:12:22Z
mutable provenance: test
owned by: james
owned by: myers
mutable description: test
discontinued: false
dominant: false
experience: experience/None
group u list: _505_
m user id: 0
owner id: 6396
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: test
star: false
creator: Mitsuhiko Odera
type: DnaRegion
role: partType/Composite
role: SO:0000804
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type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

rbs+crtZ
type: ComponentDefinition
persistent identity: BBa I742158
display id: BBa_I742158
version: 1
was generated by: iGEM to SBOL conversion
description: crtZ (beta-carotene hydroxylase) with native rbs
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to the yellow pigment zeaxnathin.
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: In stock
status: status/Available
bookmark: true
mutable notes: This part was made as a single biobrick rather than separate rbs and coding sequence, hence the use of blunt combination to generate the final sequence.
star: true
creator: Chris French
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
component: BBa_I742157
component: BBa_I742156
sequence annotation: BBa_I742156
sequence annotation: BBa_I742157
sequence: https://synbiohub.org/public/igem/BBa_I742158_sequence/1

BBa_I742157
type: Component
persistent identity: component1953815
display id: component1953815
version: 1
top level: rbs+crtZ
definition: BBa_I742157
access: public

BBa_I742156
type: Component
persistent identity: component1953813
display id: component1953813
version: 1
top level: rbs+crtZ
definition: BBa_I742156
access: public

BBa_I742156
type: SequenceAnnotation
persistent identity: annotation1953813
display id: annotation1953813
version: 1
top level: rbs+crtZ
location: https://synbiohub.org/public/igem/BBa_I742158/annotation1953813/range1953813/1
component: BBa_I742156

https://synbiohub.org/public/igem/BBa_I742158/annotation1953813/range1953813/1
type: Range
persistent identity: range1953813
display id: range1953813
version: 1
top level: rbs+crtZ
direction: direction/reverse
start: 1
end: 16
orientation: inline

BBa_I742157
type: SequenceAnnotation
persistent identity: annotation1953815
display id: annotation1953815
version: 1
top level: rbs+crtZ
location: https://synbiohub.org/public/igem/BBa_I742158/annotation1953815/range1953815/1
component: BBa_I742157

https://synbiohub.org/public/igem/BBa_I742158/annotation1953815/range1953815/1
type: Range
persistent identity: range1953815
display id: range1953815
version: 1
top level: rbs+crtZ
direction: direction/reverse
start: 17
end: 547
orientation: inline

BBa_I15008
type: ComponentDefinition
persistent identity: BBa I15008
display id: BBa_I15008
version: 1
was generated by: iGEM to SBOL conversion
description: heme oxygenase (ho1) from Synechocystis
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: One of two requisite genes required for the biosynthesis of phycocyanobilin from heme.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jeff Tabor
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15008_sequence/1

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214026
display id: annotation2214026
version: 1
top level: BBa_I15008
location: https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I15008/annotation2214026/range2214026/1
type: Range
persistent identity: range2214026
display id: range2214026
version: 1
top level: BBa_I15008
direction: direction/forward
start: 727
end: 751
orientation: inline

BBa_I15009
type: ComponentDefinition
persistent identity: BBa I15009
display id: BBa_I15009
version: 1
was generated by: iGEM to SBOL conversion
description: phycocyanobilin:ferredoxin oxidoreductase (PcyA) from synechocystis
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Second of two required phycocyanobilin biosynthetic genes.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
star: true
creator: Jeff Tabor
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: PcyA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15009_sequence/1

PcyA
type: SequenceAnnotation
persistent identity: annotation1891612
display id: annotation1891612
version: 1
top level: BBa_I15009
location: https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I15009/annotation1891612/range1891612/1
type: Range
persistent identity: range1891612
display id: range1891612
version: 1
top level: BBa_I15009
direction: direction/forward
start: 1
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214032
display id: annotation2214032
version: 1
top level: BBa_I15009
location: https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_I15009/annotation2214032/range2214032/1
type: Range
persistent identity: range2214032
display id: range2214032
version: 1
top level: BBa_I15009
direction: direction/forward
start: 751
end: 775
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa R1051
display id: BBa_R1051
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter, Standard (lambda cI regulated)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: [Note: This is the same part as R0051 except that the -10 and -35 sites and spacing have been changed to comply with BBa_S0001]. The cI regulated promoter is based on the pR promtoer from bacteriohage lambda. The promoter has two two DNA binding sites for lambda cI repressor <bb_part>BBa_C0051</bb_part>. cI binding results in repression of transcription. The specific sequence used here is based on the cI repressible promoter used in the Elowitz repressilator (and references therein).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In order to address concerns about the promoter transcribing in the reverse direction, we have removed the -35 and -10 signals responsible for the promoter activity in the reverse direction. (More details needed here! DE, 2/24/03) Modified to comply with BBa_S0001: TTGACA-17N-GATACT Incompatible with host expressing cI repressor.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross Drew Endy
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R1051
sequence annotation: OR2
sequence annotation: -35
sequence annotation: OR1
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R1051_sequence/1

BBa_R1051
type: SequenceAnnotation
persistent identity: annotation7074
display id: annotation7074
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R1051/annotation7074/range7074/1
type: Range
persistent identity: range7074
display id: range7074
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 49
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation2077
display id: annotation2077
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R1051/annotation2077/range2077/1
type: Range
persistent identity: range2077
display id: range2077
version: 1
top level: cI lam
direction: direction/forward
start: 1
end: 17
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2075
display id: annotation2075
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R1051/annotation2075/range2075/1
type: Range
persistent identity: range2075
display id: range2075
version: 1
top level: cI lam
direction: direction/forward
start: 15
end: 20
orientation: inline

OR1
type: SequenceAnnotation
persistent identity: annotation2076
display id: annotation2076
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R1051/annotation2076/range2076/1
type: Range
persistent identity: range2076
display id: range2076
version: 1
top level: cI lam
direction: direction/forward
start: 25
end: 41
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2078
display id: annotation2078
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R1051/annotation2078/range2078/1
type: Range
persistent identity: range2078
display id: range2078
version: 1
top level: cI lam
direction: direction/forward
start: 38
end: 43
orientation: inline

LacI
type: ComponentDefinition
persistent identity: BBa I732100
display id: BBa_I732100
version: 1
was generated by: iGEM to SBOL conversion
description: LacI
created: 2007-10-13T11:00:00Z
modified: 2016-10-18T06:58:21Z
mutable provenance: N/A
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: N/A
discontinued: false
dominant: false
experience: experience/Works
group u list: _156_
m user id: 31248
owner id: 1557
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: Zhan Jian
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_I732100_sequence/1

BBa_K081016
type: ComponentDefinition
persistent identity: BBa K081016
display id: BBa_K081016
version: 1
was generated by: iGEM to SBOL conversion
description: lasI protein generator - PoPS->lasI
created: 2008-10-18T11:00:00Z
modified: 2015-05-08T01:08:35Z
mutable provenance: RBS: lasI: T:
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: PoPS in -> lasI out. Strong RBS (efficiency=0.6). Artificial 39 bp terminator (efficiency=0.99).
discontinued: false
dominant: false
experience: experience/None
group u list: _227_
m user id: 0
owner id: 2583
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We used BioBrick Standard Assembly.
star: true
creator: Lorenzo Pasotti, Paolo Magni
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_B0030
component: BBa_B1006
component: BBa_C0078
sequence annotation: BBa_B0030
sequence annotation: BBa_C0078
sequence annotation: BBa_B1006
sequence: https://synbiohub.org/public/igem/BBa_K081016_sequence/1

BBa_B0030
type: Component
persistent identity: component2246157
display id: component2246157
version: 1
top level: BBa_K081016
definition: BBa_B0030
access: public

BBa_B1006
type: Component
persistent identity: component2246167
display id: component2246167
version: 1
top level: BBa_K081016
definition: BBa_B1006
access: public

BBa_C0078
type: Component
persistent identity: component2246162
display id: component2246162
version: 1
top level: BBa_K081016
definition: lasI
access: public

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation2246157
display id: annotation2246157
version: 1
top level: BBa_K081016
location: https://synbiohub.org/public/igem/BBa_K081016/annotation2246157/range2246157/1
component: BBa_B0030

https://synbiohub.org/public/igem/BBa_K081016/annotation2246157/range2246157/1
type: Range
persistent identity: range2246157
display id: range2246157
version: 1
top level: BBa_K081016
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_C0078
type: SequenceAnnotation
persistent identity: annotation2246162
display id: annotation2246162
version: 1
top level: BBa_K081016
location: https://synbiohub.org/public/igem/BBa_K081016/annotation2246162/range2246162/1
component: BBa_C0078

https://synbiohub.org/public/igem/BBa_K081016/annotation2246162/range2246162/1
type: Range
persistent identity: range2246162
display id: range2246162
version: 1
top level: BBa_K081016
direction: direction/reverse
start: 22
end: 688
orientation: inline

BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2246167
display id: annotation2246167
version: 1
top level: BBa_K081016
location: https://synbiohub.org/public/igem/BBa_K081016/annotation2246167/range2246167/1
component: BBa_B1006

https://synbiohub.org/public/igem/BBa_K081016/annotation2246167/range2246167/1
type: Range
persistent identity: range2246167
display id: range2246167
version: 1
top level: BBa_K081016
direction: direction/reverse
start: 697
end: 735
orientation: inline

BBa_K395601
type: ComponentDefinition
persistent identity: BBa K395601
display id: BBa_K395601
version: 1
was generated by: iGEM to SBOL conversion
description: T7 terminator
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:12:21Z
mutable provenance: CTAGCATAACCCCTTGGGGCCTCTAAACGGGTCTTGAGGGGTTTTTTG
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: T7 terminator
discontinued: false
dominant: false
group u list: _505_
m user id: 0
owner id: 7030
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: terminator of T7 promoter
star: false
creator: Toshitaka Matsubara
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence: https://synbiohub.org/public/igem/BBa_K395601_sequence/1

luxR
type: ComponentDefinition
persistent identity: BBa R0061
display id: BBa_R0061
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (HSL-mediated luxR repressor)
created: 2004-01-28T12:00:00Z
modified: 2015-05-08T01:14:15Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This part involves the -10 binding site, the -35 binding site, and the twenty nucleotides between that constitute the lux box. With this part, LuxR functions as a acyl-homoserine lactone-dependent repressor. LuxR resonds to the HSL produced by LuxI, N-(3-oxohexanoyl)-HSL. The Lux box is positioned such that it partially overlaps the consensus -35 and -10 hexamers of an RNA polymerase binding site.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Srini Devadas, David Gray, Ronny Krashinsky, Debra Lin, and Chris Zheng Liu
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: -35
sequence annotation: Lux Box
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0061_sequence/1

-35
type: SequenceAnnotation
persistent identity: annotation305863
display id: annotation305863
version: 1
top level: luxR
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305863/range305863/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0061/annotation305863/range305863/1
type: Range
persistent identity: range305863
display id: range305863
version: 1
top level: luxR
direction: direction/forward
start: 1
end: 6
orientation: inline

Lux Box
type: SequenceAnnotation
persistent identity: annotation305877
display id: annotation305877
version: 1
top level: luxR
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305877/range305877/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0061/annotation305877/range305877/1
type: Range
persistent identity: range305877
display id: range305877
version: 1
top level: luxR
direction: direction/forward
start: 6
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation305864
display id: annotation305864
version: 1
top level: luxR
location: https://synbiohub.org/public/igem/BBa_R0061/annotation305864/range305864/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0061/annotation305864/range305864/1
type: Range
persistent identity: range305864
display id: range305864
version: 1
top level: luxR
direction: direction/forward
start: 25
end: 30
orientation: inline

BBa_K395162
type: ComponentDefinition
persistent identity: BBa K395162
display id: BBa_K395162
version: 1
was generated by: iGEM to SBOL conversion
description: CmR activated by LuxR and 3OC6HSL(AHL of Lux family)
created: 2010-10-04T11:00:00Z
modified: 2015-05-08T01:12:21Z
owned by: james
owned by: myers
mutable description: this part express chloramphenicol resistance by the activation of LuxR and 3OC6HSL
discontinued: false
dominant: false
experience: experience/Works
group u list: _505_
m user id: 0
owner id: 6371
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: We want to create chloramphenicol resistance gene on pSB6A1.As a vector,We already create chloramphenicol resistance gene on pSB6A1and promotor exist as biobrick(BBa_R0062).To accomplish it, vector was cutted by EcoRI and Xha while insert(promotor) was cutted by EcoRI and SpeI
star: false
creator: Kaneta Yusuke
type: DnaRegion
role: SO:0000804
role: partType/Signalling
component: BBa_R0062
component: BBa_K395160
sequence annotation: BBa_R0062
sequence annotation: BBa_K395160
sequence: https://synbiohub.org/public/igem/BBa_K395162_sequence/1

BBa_R0062
type: Component
persistent identity: component2104722
display id: component2104722
version: 1
top level: BBa_K395162
definition: lux pR
access: public

BBa_K395160
type: Component
persistent identity: component2104728
display id: component2104728
version: 1
top level: BBa_K395162
definition: BBa_K395160
access: public

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation2104722
display id: annotation2104722
version: 1
top level: BBa_K395162
location: https://synbiohub.org/public/igem/BBa_K395162/annotation2104722/range2104722/1
component: BBa_R0062

https://synbiohub.org/public/igem/BBa_K395162/annotation2104722/range2104722/1
type: Range
persistent identity: range2104722
display id: range2104722
version: 1
top level: BBa_K395162
direction: direction/forward
start: 1
end: 55
orientation: inline

BBa_K395160
type: SequenceAnnotation
persistent identity: annotation2104728
display id: annotation2104728
version: 1
top level: BBa_K395162
location: https://synbiohub.org/public/igem/BBa_K395162/annotation2104728/range2104728/1
component: BBa_K395160

https://synbiohub.org/public/igem/BBa_K395162/annotation2104728/range2104728/1
type: Range
persistent identity: range2104728
display id: range2104728
version: 1
top level: BBa_K395162
direction: direction/reverse
start: 64
end: 742
orientation: inline

BBa_K395705
type: ComponentDefinition
persistent identity: BBa K395705
display id: BBa_K395705
version: 1
was generated by: iGEM to SBOL conversion
description: crtW (beta-carotene ketolase) with rbs
created: 2010-10-05T11:00:00Z
modified: 2015-05-08T01:12:22Z
mutable provenance: Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.
owned by: james
owned by: myers
mutable description: crtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin.
discontinued: false
dominant: false
experience: experience/Works
group u list: _505_
m user id: 0
owner id: 6372
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: No special considerations. Please refer to Part BBa_K395706 for more information.
star: false
creator: Yumiko Kinoshita
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
component: BBa_J15001
component: BBa_K395700
sequence annotation: BBa_J15001
sequence annotation: BBa_K395700
sequence: https://synbiohub.org/public/igem/BBa_K395705_sequence/1

BBa_J15001
type: Component
persistent identity: component2082980
display id: component2082980
version: 1
top level: BBa_K395705
definition: BBa_J15001
access: public

BBa_K395700
type: Component
persistent identity: component2082981
display id: component2082981
version: 1
top level: BBa_K395705
definition: BBa_K395700
access: public

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2082980
display id: annotation2082980
version: 1
top level: BBa_K395705
location: https://synbiohub.org/public/igem/BBa_K395705/annotation2082980/range2082980/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K395705/annotation2082980/range2082980/1
type: Range
persistent identity: range2082980
display id: range2082980
version: 1
top level: BBa_K395705
direction: direction/reverse
start: 1
end: 10
orientation: inline

BBa_K395700
type: SequenceAnnotation
persistent identity: annotation2082981
display id: annotation2082981
version: 1
top level: BBa_K395705
location: https://synbiohub.org/public/igem/BBa_K395705/annotation2082981/range2082981/1
component: BBa_K395700

https://synbiohub.org/public/igem/BBa_K395705/annotation2082981/range2082981/1
type: Range
persistent identity: range2082981
display id: range2082981
version: 1
top level: BBa_K395705
direction: direction/reverse
start: 17
end: 751
orientation: inline

BBa_K395603
type: ComponentDefinition
persistent identity: BBa K395603
display id: BBa_K395603
version: 1
was generated by: iGEM to SBOL conversion
description: alcohol acetyltransferase; converts butanol or 2-methylbutanol to butyl acetate or 2-methylbutyl ace
created: 2010-10-18T11:00:00Z
modified: 2015-05-08T01:12:22Z
mutable provenance: Apple
owned by: james
owned by: myers
mutable description: BBa_K395603 encodes alcohol acetyltransferase derived from MpAAT1 from Malus pumila cv. Royal Gala. MpAAT1 catalyzes the conversion of butanol or 2-methylbutanol to butyl acetate or 2-methylbutyl acetate. Butyl acetate and 2-methylbutyl acetate have a apple flavor.
discontinued: false
dominant: false
experience: experience/None
group u list: _505_
m user id: 0
owner id: 7030
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: RBS+lac operator+RBS+His tag+thirombin+S・tag+Factor Xa+MpAAT1+His tag+T7 terminator
star: false
creator: Toshitaka Matsubara
type: DnaRegion
role: SO:0000804
role: partType/Generator
component: BBa_K395601
component: BBa_K395604
sequence annotation: BBa_K395604
sequence annotation: BBa_K395601
sequence: https://synbiohub.org/public/igem/BBa_K395603_sequence/1

BBa_K395601
type: Component
persistent identity: component2106229
display id: component2106229
version: 1
top level: BBa_K395603
definition: BBa_K395601
access: public

BBa_K395604
type: Component
persistent identity: component2106228
display id: component2106228
version: 1
top level: BBa_K395603
definition: BBa_K395604
access: public

BBa_K395604
type: SequenceAnnotation
persistent identity: annotation2106228
display id: annotation2106228
version: 1
top level: BBa_K395603
location: https://synbiohub.org/public/igem/BBa_K395603/annotation2106228/range2106228/1
component: BBa_K395604

https://synbiohub.org/public/igem/BBa_K395603/annotation2106228/range2106228/1
type: Range
persistent identity: range2106228
display id: range2106228
version: 1
top level: BBa_K395603
direction: direction/reverse
start: 1
end: 1687
orientation: inline

BBa_K395601
type: SequenceAnnotation
persistent identity: annotation2106229
display id: annotation2106229
version: 1
top level: BBa_K395603
location: https://synbiohub.org/public/igem/BBa_K395603/annotation2106229/range2106229/1
component: BBa_K395601

https://synbiohub.org/public/igem/BBa_K395603/annotation2106229/range2106229/1
type: Range
persistent identity: range2106229
display id: range2106229
version: 1
top level: BBa_K395603
direction: direction/reverse
start: 1696
end: 1743
orientation: inline

BBa_K274200
type: ComponentDefinition
persistent identity: BBa K274200
display id: BBa_K274200
version: 1
was generated by: iGEM to SBOL conversion
description: CrtEBIY with rbs
created: 2009-09-17T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. This Composite Biobrick is created by standard assembly of Part BBa_K118014, Part BBa_K118006, Part BBa_K118005 and Part BBa_K118013.
owned by: james
owned by: myers
mutable description: This Composite Biobrick is created by standard assembly of Part BBa_K118014 (CrtE with rbs), Part BBa_K118006 (CrtB with rbs), Part BBa_K118005 (CrtI with rbs) and Part BBa_K118013 (CrtY with rbs), which are submitted by previous iGEM teams. The whole cassette is on plasmid pSB1A2 (high copy, Ampicillin resistance). Together, enzymes CrtE, CrtB, CrtI and CrtY convert colourless farnesyl pyrophosphate to orange beta-carotene (via intermediates geranylgeranyl pyroiphosphate, phytoene and lycopene). The orange beta-carotene pigment can then be used as a coloured signal output, e.g. for biosensors. There is already individual ribosome binding site before each enzyme gene sequence. Internal restriction sites have been removed by previous iGEM teams. Please refer to Parts BBa_K118014, BBa_K118006, BBa_K118005 and BBa_K118013 for more information on individual Biobricks components. This Composite Biobrick has been put under constitutive promoter R0011 (see Part BBa_K274210) and arabinose-inducible promoter I0500 (see Part BBa_274220), transformed and tested in E.coli strain MG1655. Amount of beta-carotene produced can be measured by photospectrometer with absorbance at 455nm (beta-carotene extraction using acetone). A related Composite Biobrick is Part BBa_K274100 (CrtEBI with rbs), which "stops" at one step before beta-carotene, producing (and retaining) red lycopene pigment.
discontinued: false
dominant: true
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5416
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate). Our experiences showed that this Biobrick works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment (beta-carotene) production than strain TOP10.
star: false
creator: Siming Ma
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K118002
component: BBa_J15001
component: BBa_K118003
component: BBa_I742151
component: BBa_K118012
component: BBa_K118008
sequence annotation: BBa_K118012
sequence annotation: BBa_I742151
sequence annotation: BBa_J15001
sequence annotation: BBa_K118002
sequence annotation: BBa_K118003
sequence annotation: BBa_K118008
sequence: https://synbiohub.org/public/igem/BBa_K274200_sequence/1

BBa_K118002
type: Component
persistent identity: component2021586
display id: component2021586
version: 1
top level: BBa_K274200
definition: BBa_K118002
access: public

BBa_J15001
type: Component
persistent identity: component2021584
display id: component2021584
version: 1
top level: BBa_K274200
definition: BBa_J15001
access: public

BBa_K118003
type: Component
persistent identity: component2021593
display id: component2021593
version: 1
top level: BBa_K274200
definition: BBa_K118003
access: public

BBa_J15001
type: Component
persistent identity: component2021589
display id: component2021589
version: 1
top level: BBa_K274200
definition: BBa_J15001
access: public

BBa_I742151
type: Component
persistent identity: component2021581
display id: component2021581
version: 1
top level: BBa_K274200
definition: BBa_I742151
access: public

BBa_K118012
type: Component
persistent identity: component2021580
display id: component2021580
version: 1
top level: BBa_K274200
definition: BBa_K118012
access: public

BBa_K118008
type: Component
persistent identity: component2021596
display id: component2021596
version: 1
top level: BBa_K274200
definition: BBa_K118008
access: public

BBa_K118012
type: Component
persistent identity: component2021594
display id: component2021594
version: 1
top level: BBa_K274200
definition: BBa_K118012
access: public

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021580
display id: annotation2021580
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021580/range2021580/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K274200/annotation2021580/range2021580/1
type: Range
persistent identity: range2021580
display id: range2021580
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 1
end: 4
orientation: inline

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation2021581
display id: annotation2021581
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021581/range2021581/1
component: BBa_I742151

https://synbiohub.org/public/igem/BBa_K274200/annotation2021581/range2021581/1
type: Range
persistent identity: range2021581
display id: range2021581
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 11
end: 922
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021584
display id: annotation2021584
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021584/range2021584/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274200/annotation2021584/range2021584/1
type: Range
persistent identity: range2021584
display id: range2021584
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 931
end: 940
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation2021586
display id: annotation2021586
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021586/range2021586/1
component: BBa_K118002

https://synbiohub.org/public/igem/BBa_K274200/annotation2021586/range2021586/1
type: Range
persistent identity: range2021586
display id: range2021586
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 947
end: 1879
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021589
display id: annotation2021589
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021589/range2021589/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274200/annotation2021589/range2021589/1
type: Range
persistent identity: range2021589
display id: range2021589
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 1888
end: 1897
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation2021593
display id: annotation2021593
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021593/range2021593/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K274200/annotation2021593/range2021593/1
type: Range
persistent identity: range2021593
display id: range2021593
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 1904
end: 3385
orientation: inline

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021594
display id: annotation2021594
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021594/range2021594/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K274200/annotation2021594/range2021594/1
type: Range
persistent identity: range2021594
display id: range2021594
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 3394
end: 3397
orientation: inline

BBa_K118008
type: SequenceAnnotation
persistent identity: annotation2021596
display id: annotation2021596
version: 1
top level: BBa_K274200
location: https://synbiohub.org/public/igem/BBa_K274200/annotation2021596/range2021596/1
component: BBa_K118008

https://synbiohub.org/public/igem/BBa_K274200/annotation2021596/range2021596/1
type: Range
persistent identity: range2021596
display id: range2021596
version: 1
top level: BBa_K274200
direction: direction/reverse
start: 3404
end: 4555
orientation: inline

OmpR
type: ComponentDefinition
persistent identity: BBa R0082
display id: BBa_R0082
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (OmpR, positive)
created: 2004-01-27T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: NC_000193 E. coli K12
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Positively regulated, OmpR-controlled promoter. This promoter is taken from the upstream region of ompC. Phosphorylated OmpR binds to the three operator sites and activates transcription.
discontinued: false
dominant: false
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The promoter includes the three OmpR binding sites: C1, C2, and C3. The promoter starts at -107 and goes up to the transcriptional start site at +1. An alternate version, BBa_R0083, cuts out the C2 and C3 sites. The efficacy of this promoter versus the truncated ompC upstream region (BBa_R0083) or the ompF upstream region (BBa_R0084) is currently unknown.
star: true
creator: Stephen Lee, Roshan Kumar, Joe Levine, Ziyan Chu (Polkadorks, IAP 2004)
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: C1 OmpR
sequence annotation: C2 OmpR
sequence annotation: C3 OmpR
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0082_sequence/1

C1 OmpR
type: SequenceAnnotation
persistent identity: annotation301154
display id: annotation301154
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301154/range301154/1
type: Range
persistent identity: range301154
display id: range301154
version: 1
top level: OmpR
direction: direction/forward
start: 13
end: 30
orientation: inline

C2 OmpR
type: SequenceAnnotation
persistent identity: annotation301155
display id: annotation301155
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301155/range301155/1
type: Range
persistent identity: range301155
display id: range301155
version: 1
top level: OmpR
direction: direction/forward
start: 34
end: 51
orientation: inline

C3 OmpR
type: SequenceAnnotation
persistent identity: annotation301156
display id: annotation301156
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_R0082/annotation301156/range301156/1
type: Range
persistent identity: range301156
display id: range301156
version: 1
top level: OmpR
direction: direction/forward
start: 54
end: 71
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation301166
display id: annotation301166
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0082/annotation301166/range301166/1
type: Range
persistent identity: range301166
display id: range301166
version: 1
top level: OmpR
direction: direction/forward
start: 75
end: 80
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation301167
display id: annotation301167
version: 1
top level: OmpR
location: https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0082/annotation301167/range301167/1
type: Range
persistent identity: range301167
display id: range301167
version: 1
top level: OmpR
direction: direction/forward
start: 98
end: 103
orientation: inline

cI lam
type: ComponentDefinition
persistent identity: BBa C0051
display id: BBa_C0051
version: 1
was generated by: iGEM to SBOL conversion
description: cI repressor from E. coli phage lambda (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999).
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the cI repressor based on cI repressor from bacteriophage lambda modified with an LVA tail for rapid degradation of the protein. cI repressor binds to the cI regulator (BBa_R0051).
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 References (unparsed) here: A synthetic oscillatory network of transcriptional regulators , Elowitz M.B. , Leibler S., Nature(403),335-38: 2000 Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Ian B. Dodd,1 Alison J. Perkins, Daniel Tsemitsidis, and J. Barry Egan , Genes and Development (Vol 15, No. 22) 3013-3022: 2001 BBa_C0051 cI repressor is based on the cI repressor from the Elowitz's repressilator. It has been modified to include a rapid degradation LAA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: Vinay S Mahajan, Brian Chow, Peter Carr, Voichita Marinescu and Alexander D. Wissner-Gross
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: cI lambda
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0051_sequence/1

cI lambda
type: SequenceAnnotation
persistent identity: annotation23334
display id: annotation23334
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0051/annotation23334/range23334/1
type: Range
persistent identity: range23334
display id: range23334
version: 1
top level: cI lam
direction: direction/forward
start: 4
end: 711
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation23335
display id: annotation23335
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0051/annotation23335/range23335/1
type: Range
persistent identity: range23335
display id: range23335
version: 1
top level: cI lam
direction: direction/forward
start: 712
end: 744
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213991
display id: annotation2213991
version: 1
top level: cI lam
location: https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0051/annotation2213991/range2213991/1
type: Range
persistent identity: range2213991
display id: range2213991
version: 1
top level: cI lam
direction: direction/forward
start: 751
end: 775
orientation: inline

LuxR
type: ComponentDefinition
persistent identity: BBa I0462
display id: BBa_I0462
version: 1
was generated by: iGEM to SBOL conversion
description: luxR Protein Generator
created: 2003-12-04T12:00:00Z
modified: 2015-08-31T04:07:29Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Produces LuxR protein which can sense 3OC6HSL in the media and activate transcription from R0062, the right hand Lux promoter
discontinued: false
dominant: false
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: partType/Generator
role: SO:0000804
component: BBa_B0034
component: BBa_B0010
component: BBa_C0062
component: BBa_B0012
sequence annotation: BBa_B0034
sequence annotation: BBa_C0062
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_I0462_sequence/1

BBa_B0034
type: Component
persistent identity: component943166
display id: component943166
version: 1
top level: LuxR
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component943189
display id: component943189
version: 1
top level: LuxR
definition: BBa_B0010
access: public

BBa_C0062
type: Component
persistent identity: component943181
display id: component943181
version: 1
top level: LuxR
definition: luxr
access: public

BBa_B0012
type: Component
persistent identity: component943199
display id: component943199
version: 1
top level: LuxR
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation943166
display id: annotation943166
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_I0462/annotation943166/range943166/1
type: Range
persistent identity: range943166
display id: range943166
version: 1
top level: LuxR
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation943181
display id: annotation943181
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_I0462/annotation943181/range943181/1
type: Range
persistent identity: range943181
display id: range943181
version: 1
top level: LuxR
direction: direction/forward
start: 19
end: 774
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation943189
display id: annotation943189
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_I0462/annotation943189/range943189/1
type: Range
persistent identity: range943189
display id: range943189
version: 1
top level: LuxR
direction: direction/forward
start: 808
end: 887
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation943199
display id: annotation943199
version: 1
top level: LuxR
location: https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_I0462/annotation943199/range943199/1
type: Range
persistent identity: range943199
display id: range943199
version: 1
top level: LuxR
direction: direction/forward
start: 896
end: 936
orientation: inline

BBa_J23107
type: ComponentDefinition
persistent identity: BBa J23107
display id: BBa_J23107
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-16T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Later
owned by: james
owned by: myers
mutable description: Later
discontinued: false
dominant: false
experience: experience/Works
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23107_sequence/1

lacI+pL
type: ComponentDefinition
persistent identity: BBa R0011
display id: BBa_R0011
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (lacI regulated, lambda pL hybrid)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: represillator of Elowitz and Leibler (2000)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site. LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
star: true
creator: Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0011
sequence annotation: lac O1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0011_sequence/1

BBa_R0011
type: SequenceAnnotation
persistent identity: annotation7064
display id: annotation7064
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0011/annotation7064/range7064/1
type: Range
persistent identity: range7064
display id: range7064
version: 1
top level: lacI+pL
direction: direction/forward
start: 1
end: 54
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation1999
display id: annotation1999
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation1999/range1999/1
type: Range
persistent identity: range1999
display id: range1999
version: 1
top level: lacI+pL
direction: direction/forward
start: 3
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2000
display id: annotation2000
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2000/range2000/1
type: Range
persistent identity: range2000
display id: range2000
version: 1
top level: lacI+pL
direction: direction/forward
start: 20
end: 25
orientation: inline

lac O1
type: SequenceAnnotation
persistent identity: annotation2001
display id: annotation2001
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_R0011/annotation2001/range2001/1
type: Range
persistent identity: range2001
display id: range2001
version: 1
top level: lacI+pL
direction: direction/forward
start: 26
end: 42
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2002
display id: annotation2002
version: 1
top level: lacI+pL
location: https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0011/annotation2002/range2002/1
type: Range
persistent identity: range2002
display id: range2002
version: 1
top level: lacI+pL
direction: direction/forward
start: 43
end: 48
orientation: inline

BBa_I15010
type: ComponentDefinition
persistent identity: BBa I15010
display id: BBa_I15010
version: 1
was generated by: iGEM to SBOL conversion
description: cph8 (Cph1/EnvZ fusion)
created: 2004-09-19T11:00:00Z
modified: 2015-08-31T04:07:38Z
mutable provenance: Voigt Lab via Anselm Levskaya
owned by: james
owned by: myers
mutable description: Chimeric Cph1 light receptor/EnvZ protein.
discontinued: false
dominant: false
experience: experience/None
group u list: _5_
m user id: 0
owner id: 88
owning group id: 7
sample status: In stock
status: status/Available
bookmark: true
mutable notes: Silent mutation at base 108 (G-A) to remove PstI site
star: false
creator: Jeff Tabor
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Cph8
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_I15010_sequence/1

Cph8
type: SequenceAnnotation
persistent identity: annotation1891613
display id: annotation1891613
version: 1
top level: BBa_I15010
location: https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I15010/annotation1891613/range1891613/1
type: Range
persistent identity: range1891613
display id: range1891613
version: 1
top level: BBa_I15010
direction: direction/forward
start: 1
end: 2238
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214031
display id: annotation2214031
version: 1
top level: BBa_I15010
location: https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_I15010/annotation2214031/range2214031/1
type: Range
persistent identity: range2214031
display id: range2214031
version: 1
top level: BBa_I15010
direction: direction/forward
start: 2239
end: 2263
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa Q04400
display id: BBa_Q04400
version: 1
was generated by: iGEM to SBOL conversion
description: QPI (B0034.C0040.B0015.R0040)
created: 2003-12-02T12:00:00Z
modified: 2015-05-08T01:14:13Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: TetR QPI with strong RBS
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Caitlin Conboy and Jennifer Braff
type: DnaRegion
role: SO:0000804
role: partType/Inverter
component: BBa_B0010
component: BBa_C0040
component: BBa_R0040
component: BBa_B0012
component: BBa_B0034
sequence annotation: BBa_B0034
sequence annotation: BBa_C0040
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence: https://synbiohub.org/public/igem/BBa_Q04400_sequence/1

BBa_B0010
type: Component
persistent identity: component952797
display id: component952797
version: 1
top level: tetR
definition: BBa_B0010
access: public

BBa_C0040
type: Component
persistent identity: component952791
display id: component952791
version: 1
top level: tetR
definition: tetR
access: public

BBa_R0040
type: Component
persistent identity: component952822
display id: component952822
version: 1
top level: tetR
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component952807
display id: component952807
version: 1
top level: tetR
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component952781
display id: component952781
version: 1
top level: tetR
definition: BBa_B0034
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation952781
display id: annotation952781
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952781/range952781/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_Q04400/annotation952781/range952781/1
type: Range
persistent identity: range952781
display id: range952781
version: 1
top level: tetR
direction: direction/forward
start: 1
end: 12
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation952791
display id: annotation952791
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952791/range952791/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_Q04400/annotation952791/range952791/1
type: Range
persistent identity: range952791
display id: range952791
version: 1
top level: tetR
direction: direction/forward
start: 19
end: 678
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation952797
display id: annotation952797
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952797/range952797/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_Q04400/annotation952797/range952797/1
type: Range
persistent identity: range952797
display id: range952797
version: 1
top level: tetR
direction: direction/forward
start: 712
end: 791
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation952807
display id: annotation952807
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952807/range952807/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_Q04400/annotation952807/range952807/1
type: Range
persistent identity: range952807
display id: range952807
version: 1
top level: tetR
direction: direction/forward
start: 800
end: 840
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation952822
display id: annotation952822
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_Q04400/annotation952822/range952822/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_Q04400/annotation952822/range952822/1
type: Range
persistent identity: range952822
display id: range952822
version: 1
top level: tetR
direction: direction/forward
start: 849
end: 902
orientation: inline

BBa_K395407
type: ComponentDefinition
persistent identity: BBa K395407
display id: BBa_K395407
version: 1
was generated by: iGEM to SBOL conversion
description: pompC(CS1)-lacIM1
created: 2010-10-04T11:00:00Z
modified: 2015-05-08T01:12:21Z
mutable provenance: test
owned by: james
owned by: myers
mutable description: lacIM1 regulated by ompC-CS1 promoter.The promoter is regulated by phosphorylated OmpR.
discontinued: false
dominant: false
experience: experience/None
group u list: _505_
m user id: 0
owner id: 6396
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: test
star: false
creator: Mitsuhiko Odera
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_K395303
component: BBa_K082005
component: BBa_B0034
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_K395303
sequence annotation: BBa_B0034
sequence annotation: BBa_K082005
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_K395407_sequence/1

BBa_K395303
type: Component
persistent identity: component2082491
display id: component2082491
version: 1
top level: BBa_K395407
definition: PompC-CS1
access: public

BBa_K082005
type: Component
persistent identity: component2082494
display id: component2082494
version: 1
top level: BBa_K395407
definition: BBa_K082005
access: public

BBa_B0034
type: Component
persistent identity: component2082493
display id: component2082493
version: 1
top level: BBa_K395407
definition: BBa_B0034
access: public

BBa_B0012
type: Component
persistent identity: component2082497
display id: component2082497
version: 1
top level: BBa_K395407
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component2082495
display id: component2082495
version: 1
top level: BBa_K395407
definition: BBa_B0010
access: public

BBa_K395303
type: SequenceAnnotation
persistent identity: annotation2082491
display id: annotation2082491
version: 1
top level: BBa_K395407
location: https://synbiohub.org/public/igem/BBa_K395407/annotation2082491/range2082491/1
component: BBa_K395303

https://synbiohub.org/public/igem/BBa_K395407/annotation2082491/range2082491/1
type: Range
persistent identity: range2082491
display id: range2082491
version: 1
top level: BBa_K395407
direction: direction/reverse
start: 1
end: 59
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2082493
display id: annotation2082493
version: 1
top level: BBa_K395407
location: https://synbiohub.org/public/igem/BBa_K395407/annotation2082493/range2082493/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K395407/annotation2082493/range2082493/1
type: Range
persistent identity: range2082493
display id: range2082493
version: 1
top level: BBa_K395407
direction: direction/reverse
start: 68
end: 79
orientation: inline

BBa_K082005
type: SequenceAnnotation
persistent identity: annotation2082494
display id: annotation2082494
version: 1
top level: BBa_K395407
location: https://synbiohub.org/public/igem/BBa_K395407/annotation2082494/range2082494/1
component: BBa_K082005

https://synbiohub.org/public/igem/BBa_K395407/annotation2082494/range2082494/1
type: Range
persistent identity: range2082494
display id: range2082494
version: 1
top level: BBa_K395407
direction: direction/reverse
start: 86
end: 1168
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2082495
display id: annotation2082495
version: 1
top level: BBa_K395407
location: https://synbiohub.org/public/igem/BBa_K395407/annotation2082495/range2082495/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K395407/annotation2082495/range2082495/1
type: Range
persistent identity: range2082495
display id: range2082495
version: 1
top level: BBa_K395407
direction: direction/forward
start: 1177
end: 1256
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2082497
display id: annotation2082497
version: 1
top level: BBa_K395407
location: https://synbiohub.org/public/igem/BBa_K395407/annotation2082497/range2082497/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K395407/annotation2082497/range2082497/1
type: Range
persistent identity: range2082497
display id: range2082497
version: 1
top level: BBa_K395407
direction: direction/forward
start: 1265
end: 1305
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_I742156
type: ComponentDefinition
persistent identity: BBa I742156
display id: BBa_I742156
version: 1
was generated by: iGEM to SBOL conversion
description: crtZ native rbs
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
mutable description: Native ribosome binding site for crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
discontinued: false
dominant: false
experience: experience/None
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: A virtual part to be added to the coding sequence blunt (without a scar).
star: false
creator: Chris French
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_I742156_sequence/1

BBa_I742157
type: ComponentDefinition
persistent identity: BBa I742157
display id: BBa_I742157
version: 1
was generated by: iGEM to SBOL conversion
description: crtZ (beta-carotene hydroxylase) coding sequence
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
mutable description: Coding sequence for crtZ (beta-carotene hydroxylase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to the yellow pigment zeaxanthin.
discontinued: false
dominant: true
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No special considerations.
star: false
creator: Chris French
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: crtZ
sequence: https://synbiohub.org/public/igem/BBa_I742157_sequence/1

crtZ
type: SequenceAnnotation
persistent identity: annotation1953812
display id: annotation1953812
version: 1
top level: BBa_I742157
location: https://synbiohub.org/public/igem/BBa_I742157/annotation1953812/range1953812/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I742157/annotation1953812/range1953812/1
type: Range
persistent identity: range1953812
display id: range1953812
version: 1
top level: BBa_I742157
direction: direction/forward
start: 1
end: 528
orientation: inline

BBa_B0030
type: ComponentDefinition
persistent identity: BBa B0030
display id: BBa_B0030
version: 1
was generated by: iGEM to SBOL conversion
description: RBS.1 (strong) -- modified from R. Weiss
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Strong RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0032</bb_part>, <bb_part>BBa_B0033</bb_part>.
discontinued: false
dominant: true
experience: experience/Works
group u list: _44_46_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("orig" in figure 4-14 of Ron Weiss thesis). No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: RBS-1\Strong
sequence annotation: BBa_B0030
sequence annotation: RBS
sequence: https://synbiohub.org/public/igem/BBa_B0030_sequence/1

RBS-1\Strong
type: SequenceAnnotation
persistent identity: annotation1701
display id: annotation1701
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_B0030/annotation1701/range1701/1
type: Range
persistent identity: range1701
display id: range1701
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

BBa_B0030
type: SequenceAnnotation
persistent identity: annotation7025
display id: annotation7025
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0030/annotation7025/range7025/1
type: Range
persistent identity: range7025
display id: range7025
version: 1
top level: BBa_B0030
direction: direction/forward
start: 1
end: 15
orientation: inline

RBS
type: SequenceAnnotation
persistent identity: annotation1702
display id: annotation1702
version: 1
top level: BBa_B0030
location: https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_B0030/annotation1702/range1702/1
type: Range
persistent identity: range1702
display id: range1702
version: 1
top level: BBa_B0030
direction: direction/forward
start: 8
end: 12
orientation: inline

lux pR
type: ComponentDefinition
persistent identity: BBa R0062
display id: BBa_R0062
version: 1
was generated by: iGEM to SBOL conversion
description: Promoter (luxR & HSL regulated -- lux pR)
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:15Z
mutable provenance: V. fischeri
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Promoter activated by LuxR in concert with HSL The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: This promoter is based on the Vibrio fischeri quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <img src="<bb_file>Image1.gif</bb_file>" width="614" height="362">
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: BBa_R0062
sequence annotation: LuxR/HSL
sequence annotation: -35
sequence annotation: -10
sequence annotation: start
sequence: https://synbiohub.org/public/igem/BBa_R0062_sequence/1

BBa_R0062
type: SequenceAnnotation
persistent identity: annotation7070
display id: annotation7070
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0062/annotation7070/range7070/1
type: Range
persistent identity: range7070
display id: range7070
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 55
orientation: inline

LuxR/HSL
type: SequenceAnnotation
persistent identity: annotation2045
display id: annotation2045
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0062/annotation2045/range2045/1
type: Range
persistent identity: range2045
display id: range2045
version: 1
top level: lux pR
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2046
display id: annotation2046
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2046/range2046/1
type: Range
persistent identity: range2046
display id: range2046
version: 1
top level: lux pR
direction: direction/forward
start: 20
end: 25
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2047
display id: annotation2047
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0062/annotation2047/range2047/1
type: Range
persistent identity: range2047
display id: range2047
version: 1
top level: lux pR
direction: direction/forward
start: 42
end: 47
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2048
display id: annotation2048
version: 1
top level: lux pR
location: https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_R0062/annotation2048/range2048/1
type: Range
persistent identity: range2048
display id: range2048
version: 1
top level: lux pR
direction: direction/forward
start: 53
end: 53
orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
star: false
creator: Chris French
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
top level: BBa_J15001
direction: direction/forward
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
top level: BBa_J15001
direction: direction/forward
start: 4
end: 10
orientation: inline

BBa_I742151
type: ComponentDefinition
persistent identity: BBa I742151
display id: BBa_I742151
version: 1
was generated by: iGEM to SBOL conversion
description: crtE (geranylgeranyl pyrophosphate synthase) coding sequence.
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
mutable description: crtE (geranylgeranyl pyrophosphate synthase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis parthway.
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No important considerations.
star: false
creator: Chris French
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence: https://synbiohub.org/public/igem/BBa_I742151_sequence/1

lasI
type: ComponentDefinition
persistent identity: BBa C0078
display id: BBa_C0078
version: 1
was generated by: iGEM to SBOL conversion
description: autoinducer synthetase for PAI from Pseudomonas aeruginosa
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: www.ncbi.nlm.nih.gov
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for lasI protein, which produces the chemical signal AI-1
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Chris Walsh (Fighting Darwins)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: LasI
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0078_sequence/1

LasI
type: SequenceAnnotation
persistent identity: annotation305970
display id: annotation305970
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0078/annotation305970/range305970/1
type: Range
persistent identity: range305970
display id: range305970
version: 1
top level: lasI
direction: direction/forward
start: 1
end: 603
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation306608
display id: annotation306608
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0078/annotation306608/range306608/1
type: Range
persistent identity: range306608
display id: range306608
version: 1
top level: lasI
direction: direction/forward
start: 604
end: 636
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2214003
display id: annotation2214003
version: 1
top level: lasI
location: https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0078/annotation2214003/range2214003/1
type: Range
persistent identity: range2214003
display id: range2214003
version: 1
top level: lasI
direction: direction/forward
start: 643
end: 667
orientation: inline

BBa_K118002
type: ComponentDefinition
persistent identity: BBa K118002
display id: BBa_K118002
version: 1
was generated by: iGEM to SBOL conversion
description: crtB coding sequence encoding phytoene synthase
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtB from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene. Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12), 6704-6712 (1990)
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations.
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: crtB coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118002_sequence/1

crtB coding sequence
type: SequenceAnnotation
persistent identity: annotation1995701
display id: annotation1995701
version: 1
top level: BBa_K118002
location: https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1
type: Range
persistent identity: range1995701
display id: range1995701
version: 1
top level: BBa_K118002
direction: direction/forward
start: 1
end: 930
orientation: inline

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

BBa_K118012
type: ComponentDefinition
persistent identity: BBa K118012
display id: BBa_K118012
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic ribosome binding site added by Son of Babel procedure
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
mutable description: This is a 4bp synthetic ribosome binding site which can be added by fusion PCR using a reverse primer and the Son of Babel cloning system.
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The part can be added by fusion PCR using a Son of Babel reverse primer.
star: false
creator: Andrew Hall
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_K118012_sequence/1

BBa_K395160
type: ComponentDefinition
persistent identity: BBa K395160
display id: BBa_K395160
version: 1
was generated by: iGEM to SBOL conversion
description: RBS-CmR(no promoter and terminator)
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:12:21Z
mutable provenance: aaaatatatcatca tgaacaataaaactgtctgcttacataaacagtaatacaaggggtgttatgagccatattcaacgggaaacgtcttgctcccgtccgcgcttaaactcca acatggacgctgatttatatgggtataaatgggctcgcgataatgtcgggcaatcaggtgcgacaatctatcgcttgtatgggaagcccgatgcgccaga gttgtttctgaaacatggcaaaggtagcgttgccaatgatgttacagatgagatggtccgtctcaactggctgacggagtttatgcctctcccgaccatc aagcattttatccgtactcctgatgatgcgtggttactcaccaccgcgattcctgggaaaacagccttccaggtattagaagaatatcctgattcaggtg aaaatattgttgatgcgctggccgtgttcctgcgccggttacattcgattcctgtttgtaattgtccttttaacagcgatcgtgtatttcgtcttgctca ggcgcaatcacgcatgaataacggtttggttgatgcgagtgattttgatgacgagcgtaatggctggcctgttgaacaagtctggaaagaaatgcacaag ctcttgccattctcaccggattcagtcgtcactcatggtgatttctcacttgataaccttatttttgacgaggggaaattaataggttgtattgatgttg gacgggtcggaatcgcagaccgttaccaggaccttgccattctttggaactgcctcggtgagttttctccttcattacagaaacggctttttcaaaaata tggtattgataatcctgatatgaataaattgcagtttcatttgatgctcgatgagtttttctaataa
owned by: james
owned by: myers
mutable description: We get this part ,RBS-CmR, from BBa_P1004 by PCR. this doesn't have any promoters and terminators.
discontinued: false
dominant: false
experience: experience/Works
group u list: _505_
m user id: 0
owner id: 6371
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: We want to create promoterless CmR on pSB6A1. so We get this part ,RBS-CmR, from BBa_P1004 by PCR and then ligated with pSB6A1
star: false
creator: Kaneta Yusuke
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: RBS
sequence annotation: CmR
sequence: https://synbiohub.org/public/igem/BBa_K395160_sequence/1

RBS
type: SequenceAnnotation
persistent identity: annotation2081191
display id: annotation2081191
version: 1
top level: BBa_K395160
location: https://synbiohub.org/public/igem/BBa_K395160/annotation2081191/range2081191/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_K395160/annotation2081191/range2081191/1
type: Range
persistent identity: range2081191
display id: range2081191
version: 1
top level: BBa_K395160
direction: direction/forward
start: 6
end: 9
orientation: inline

CmR
type: SequenceAnnotation
persistent identity: annotation2081192
display id: annotation2081192
version: 1
top level: BBa_K395160
location: https://synbiohub.org/public/igem/BBa_K395160/annotation2081192/range2081192/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K395160/annotation2081192/range2081192/1
type: Range
persistent identity: range2081192
display id: range2081192
version: 1
top level: BBa_K395160
direction: direction/forward
start: 17
end: 677
orientation: inline

BBa_K395700
type: ComponentDefinition
persistent identity: BBa K395700
display id: BBa_K395700
version: 1
was generated by: iGEM to SBOL conversion
description: crtW (beta-carotene ketolase) coding sequence
created: 2010-10-04T11:00:00Z
modified: 2015-05-08T01:12:22Z
mutable provenance: Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.
owned by: james
owned by: myers
mutable description: Coding sequence for crtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271. Part of the carotenoid biosynthesis pathway. Converts beta-carotene to canthaxanthin and zeaxanthin to astaxanthin.
discontinued: false
dominant: false
group u list: _505_
m user id: 0
owner id: 6372
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No special considerations. Please refer to Part BBa_K395706 for more information.
star: false
creator: Yumiko Kinoshita
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_K395700_sequence/1

BBa_K395604
type: ComponentDefinition
persistent identity: BBa K395604
display id: BBa_K395604
version: 1
was generated by: iGEM to SBOL conversion
description: MpAAT1 coding region
created: 2010-10-25T11:00:00Z
modified: 2015-05-08T01:12:22Z
mutable provenance: ATGA TGTCCTTTTC CGTACTGCAA GTGAAACGTC TGCAACCGGA 301 ACTGATTACA CCGGCAAAAT CAACCCCTCA GGAAACCAAA TTTCTGAGCG ACATCGATGA 361 TCAAGAATCT CTGCGCGTTC AGATTCCGAT TATCATGTGT TATAAAGACA ACCCGAGCCT 421 GAATAAAAAC CGTAACCCGG TCAAAGCCAT TCGTGAAGCA CTGTCACGTG CCCTGGTCTA 481 TTATTATCCA CTGGCCGGTC GTCTGCGTGA AGGTCCAAAC CGTAAACTGG TGGTCGATTG 541 TAACGGCGAA GGCATCCTGT TTGTGGAAGC TTCCGCCGAT GTTACGCTGG AACAGCTGGG 601 GGACAAAATC CTGCCTCCTT GTCCTCTGCT GGAGGAGTTT CTGTATAACT TCCCGGGTTC 661 TGATGGCATT ATCGACTGCC CACTGCTGCT GATTCAAGTG ACCTGTCTGA CTTGTGGAGG 721 GTTCATCCTG GCTCTGCGCC TGAACCATAC AATGTGTGAT GCCGCCGGTC TGCTGCTGTT 781 TCTGACCGCT ATTGCCGAAA TGGCCCGTGG CGCTCATGCC CCTAGTATTC TGCCTGTCTG 841 GGAGCGTGAA CTGCTGTTTG CTCGTGATCC TCCTCGTATC ACCTGTGCCC ACCATGAATA 901 TGAGGACGTG ATTGGCCACT CAGATGGGTC TTATGCCAGC TCAAATCAGA GTAATATGGT 961 CCAACGCTCG TTCTATTTTG GCGCCAAAGA GATGCGCGTA CTGCGTAAAC AAATTCCGCC 1021 ACACCTGATT AGCACTTGTA GCACCTTCGA TCTGATCACC GCTTGTCTGT GGAAATGTCG 1081 TACCCTGGCA CTGAACATTA ACCCGAAAGA GGCCGTTCGT GTTTCGTGTA TTGTGAATGC 1141 TCGTGGTAAA CACAACAACG TTCGCCTGCC TCTGGGTTAT TATGGTAACG CCTTTGCCTT 1201 TCCGGCAGCA ATTTCAAAAG CCGAACCTCT GTGTAAAAAC CCGCTGGGAT ATGCTCTGGA 1261 ACTGGTAAAA AAAGCGAAAG CGACTATGAA CGAAGAGTAT CTGCGTTCTG TTGCTGATCT 1321 GCTGGTACTG CGCGGTCGCC CTCAGTATAG TAGTACCGGC TCCTATCTGA TCGTTAGCGA 1381 CAACACCCGT GTAGGCTTCG GAGATGTGAA TTTTGGCTGG GGTCAGCCTG TTTTTGCTGG 1441 ACCGGTTAAA GCACTGGATC TGATCTCGTT TTATGTCCAA CACAAAAACA ACACCGAGGA 1501 TGGCATTCTG GTTCCGATGT GTCTGCCTAG TAGCGCTATG GAACGCTTTC AGCAGGAACT 1561 GGAGCGTATT ACCCAGGAAC CAAAAGAGGA CATCTGTAAC AATCTGCGCT CAACGTCCCA 1621 GTAA
owned by: james
owned by: myers
mutable description: MpAAT1 coding region
discontinued: false
dominant: false
experience: experience/None
group u list: _505_
m user id: 0
owner id: 7030
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: a
star: false
creator: Toshitaka Matsubara
type: DnaRegion
role: partType/Translational Unit
role: SO:0000804
sequence annotation: rbs
sequence annotation: start
sequence annotation: his-tag
sequence annotation: s-tag
sequence annotation: factor-Xa
sequence annotation: MpAAT1
sequence annotation: stop
sequence annotation: His-tag
sequence: https://synbiohub.org/public/igem/BBa_K395604_sequence/1

rbs
type: SequenceAnnotation
persistent identity: annotation2106199
display id: annotation2106199
version: 1
top level: BBa_K395604
location: https://synbiohub.org/public/igem/BBa_K395604/annotation2106199/range2106199/1
role: feature/rbs
role: SO:0000139

https://synbiohub.org/public/igem/BBa_K395604/annotation2106199/range2106199/1
type: Range
persistent identity: range2106199
display id: range2106199
version: 1
top level: BBa_K395604
direction: direction/forward
start: 10
end: 14
orientation: inline

start
type: SequenceAnnotation
persistent identity: annotation2106200
display id: annotation2106200
version: 1
top level: BBa_K395604
location: https://synbiohub.org/public/igem/BBa_K395604/annotation2106200/range2106200/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K395604/annotation2106200/range2106200/1
type: Range
persistent identity: range2106200
display id: range2106200
version: 1
top level: BBa_K395604
direction: direction/forward
start: 24
end: 24
orientation: inline

his-tag
type: SequenceAnnotation
persistent identity: annotation2106201
display id: annotation2106201
version: 1
top level: BBa_K395604
location: https://synbiohub.org/public/igem/BBa_K395604/annotation2106201/range2106201/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K395604/annotation2106201/range2106201/1
type: Range
persistent identity: range2106201
display id: range2106201
version: 1
top level: BBa_K395604
direction: direction/forward
start: 27
end: 44
orientation: inline

s-tag
type: SequenceAnnotation
persistent identity: annotation2106202
display id: annotation2106202
version: 1
top level: BBa_K395604
location: https://synbiohub.org/public/igem/BBa_K395604/annotation2106202/range2106202/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K395604/annotation2106202/range2106202/1
type: Range
persistent identity: range2106202
display id: range2106202
version: 1
top level: BBa_K395604
direction: direction/forward
start: 78
end: 111
orientation: inline

factor-Xa
type: SequenceAnnotation
persistent identity: annotation2106203
display id: annotation2106203
version: 1
top level: BBa_K395604
location: https://synbiohub.org/public/igem/BBa_K395604/annotation2106203/range2106203/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K395604/annotation2106203/range2106203/1
type: Range
persistent identity: range2106203
display id: range2106203
version: 1
top level: BBa_K395604
direction: direction/forward
start: 153
end: 164
orientation: inline

MpAAT1
type: SequenceAnnotation
persistent identity: annotation2106204
display id: annotation2106204
version: 1
top level: BBa_K395604
location: https://synbiohub.org/public/igem/BBa_K395604/annotation2106204/range2106204/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K395604/annotation2106204/range2106204/1
type: Range
persistent identity: range2106204
display id: range2106204
version: 1
top level: BBa_K395604
direction: direction/forward
start: 165
end: 1532
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation2106205
display id: annotation2106205
version: 1
top level: BBa_K395604
location: https://synbiohub.org/public/igem/BBa_K395604/annotation2106205/range2106205/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_K395604/annotation2106205/range2106205/1
type: Range
persistent identity: range2106205
display id: range2106205
version: 1
top level: BBa_K395604
direction: direction/forward
start: 1532
end: 1532
orientation: inline

His-tag
type: SequenceAnnotation
persistent identity: annotation2106219
display id: annotation2106219
version: 1
top level: BBa_K395604
location: https://synbiohub.org/public/igem/BBa_K395604/annotation2106219/range2106219/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K395604/annotation2106219/range2106219/1
type: Range
persistent identity: range2106219
display id: range2106219
version: 1
top level: BBa_K395604
direction: direction/forward
start: 1611
end: 1628
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

BBa_K118003
type: ComponentDefinition
persistent identity: BBa K118003
display id: BBa_K118003
version: 1
was generated by: iGEM to SBOL conversion
description: crtI coding sequence encoding phytoene dehydrogenase
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows: * crtImutf1: gtt tac agt aag gtt gcc * crtImutf2: agt aac tct ctg ttt gtg c In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: crtI coding sequence
sequence annotation: PstI site removed
sequence: https://synbiohub.org/public/igem/BBa_K118003_sequence/1

crtI coding sequence
type: SequenceAnnotation
persistent identity: annotation1995702
display id: annotation1995702
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
type: Range
persistent identity: range1995702
display id: range1995702
version: 1
top level: BBa_K118003
direction: direction/forward
start: 1
end: 1479
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968244
display id: annotation1968244
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
type: Range
persistent identity: range1968244
display id: range1968244
version: 1
top level: BBa_K118003
direction: direction/forward
start: 478
end: 483
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968245
display id: annotation1968245
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
type: Range
persistent identity: range1968245
display id: range1968245
version: 1
top level: BBa_K118003
direction: direction/forward
start: 889
end: 894
orientation: inline

BBa_K118008
type: ComponentDefinition
persistent identity: BBa K118008
display id: BBa_K118008
version: 1
was generated by: iGEM to SBOL conversion
description: crtY coding sequence encoding lycopene B-cyclase
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtY from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes lycopene B-cyclase, part of the carotenoid biosynthesis pathway, which converts lycopene to B-carotene.
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: crtY coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118008_sequence/1

crtY coding sequence
type: SequenceAnnotation
persistent identity: annotation1995716
display id: annotation1995716
version: 1
top level: BBa_K118008
location: https://synbiohub.org/public/igem/BBa_K118008/annotation1995716/range1995716/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118008/annotation1995716/range1995716/1
type: Range
persistent identity: range1995716
display id: range1995716
version: 1
top level: BBa_K118008
direction: direction/forward
start: 1
end: 1149
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

PompC-CS1
type: ComponentDefinition
persistent identity: BBa K395303
display id: BBa_K395303
version: 1
was generated by: iGEM to SBOL conversion
description: PompC(CS1) Promoter regulated by phosphorylated OmpR
created: 2010-10-01T11:00:00Z
modified: 2015-05-08T01:12:21Z
mutable provenance: ATTTACATTTTGAAACATCTGACCGTTGGATTATTCTGCATTTTTGGGGAGAATGGACT
owned by: james
owned by: myers
mutable description: test
discontinued: false
dominant: false
experience: experience/Works
group u list: _505_
m user id: 0
owner id: 7027
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: test test
star: false
creator: Taichi Nakamura
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Binding site1
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_K395303_sequence/1

Binding site1
type: SequenceAnnotation
persistent identity: annotation2081250
display id: annotation2081250
version: 1
top level: PompC-CS1
location: https://synbiohub.org/public/igem/BBa_K395303/annotation2081250/range2081250/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_K395303/annotation2081250/range2081250/1
type: Range
persistent identity: range2081250
display id: range2081250
version: 1
top level: PompC-CS1
direction: direction/forward
start: 1
end: 20
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation2090359
display id: annotation2090359
version: 1
top level: PompC-CS1
location: https://synbiohub.org/public/igem/BBa_K395303/annotation2090359/range2090359/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_K395303/annotation2090359/range2090359/1
type: Range
persistent identity: range2090359
display id: range2090359
version: 1
top level: PompC-CS1
direction: direction/forward
start: 26
end: 31
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation2102148
display id: annotation2102148
version: 1
top level: PompC-CS1
location: https://synbiohub.org/public/igem/BBa_K395303/annotation2102148/range2102148/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_K395303/annotation2102148/range2102148/1
type: Range
persistent identity: range2102148
display id: range2102148
version: 1
top level: PompC-CS1
direction: direction/forward
start: 49
end: 54
orientation: inline

BBa_K082005
type: ComponentDefinition
persistent identity: BBa K082005
display id: BBa_K082005
version: 1
was generated by: iGEM to SBOL conversion
description: LacI-Mutant
created: 2008-10-27T12:00:00Z
modified: 2015-05-08T01:08:35Z
mutable provenance: None
owned by: james
owned by: myers
mutable description: None
discontinued: false
dominant: false
experience: experience/Works
group u list: _228_
m user id: 0
owner id: 1560
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Yanting Xue, Binjie Xu
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: BBa_K082005
sequence: https://synbiohub.org/public/igem/BBa_K082005_sequence/1

BBa_K082005
type: SequenceAnnotation
persistent identity: annotation1992781
display id: annotation1992781
version: 1
top level: BBa_K082005
location: https://synbiohub.org/public/igem/BBa_K082005/annotation1992781/range1992781/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_K082005/annotation1992781/range1992781/1
type: Range
persistent identity: range1992781
display id: range1992781
version: 1
top level: BBa_K082005
direction: direction/reverse
start: 1
end: 1083
orientation: inline

https://synbiohub.org/public/igem/BBa_K395998_sequence/1
type: Sequence
persistent identity: BBa K395998 sequence
display id: BBa_K395998_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagccctaggtattatgctagctactagagaaagaggagaaatactagatgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgctactagagaaagaggagaaatactagatggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtttacggctagctcagccctaggtattatgctagctactagagaaagaggagaaatactagatggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742158_sequence/1
type: Sequence
persistent identity: BBa I742158 sequence
display id: BBa_I742158_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctctaccggagaaattatgttgtggatttggaatgccctgatcgttttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggcatctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgatcctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactctattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggttgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcccctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggaggatgagcccgcatccgggaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15008_sequence/1
type: Sequence
persistent identity: BBa I15008 sequence
display id: BBa_I15008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtgtcaacttagcttcccagttgcgggaagggacgaaaaaatcccactccatggcggagaacgtcggctttgtcaaatgcttcctcaagggcgttgtcgagaaaaattcctaccgtaagctggttggcaatctctactttgtctacagtgccatggaagaggaaatggcaaaatttaaggaccatcccatcctcagccacatttacttccccgaactcaaccgcaaacaaagcctagagcaagacctgcaattctattacggctccaactggcggcaagaagtgaaaatttctgccgctggccaagcctatgtggaccgagtccggcaagtggccgctacggcccctgaattgttggtggcccattcctacacccgttacctgggggatctttccggcggtcaaattctcaagaaaattgcccaaaatgccatgaatctccacgatggtggcacagctttctatgaatttgccgacattgatgacgaaaaggcttttaaaaatacctaccgtcaagctatgaatgatctgcccattgaccaagccaccgccgaacggattgtggatgaagccaatgacgcctttgccatgaacatgaaaatgttcaacgaacttgaaggcaacctgatcaaggcgatcggcattatggtgttcaacagcctcacccgtcgccgcagtcaaggcagcaccgaagttggcctcgccacctccgaaggctaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15009_sequence/1
type: Sequence
persistent identity: BBa I15009 sequence
display id: BBa_I15009_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccgtcactgatttaagtttgaccaattcttccctgatgcctacgttgaacccgatgattcaacagttggccctggcgatcgccgctagttggcaaagtttacccctcaagccctatcaattgccggaggatttgggctacgtagaaggccgcctggaaggggaaaagttagtgattgaaaatcggtgctaccaaacgccccagtttcgcaaaatgcatttggagttggccaaggtgggcaaagggttggatattctccactgtgtaatgtttcctgagcctttatacggtctacctttgtttggctgtgacattgtggccggccccggtggagtaagtgcggctattgcggatctatcccccacccaaagcgatcgccaattgcccgcagcgtaccaaaaatcattggcagagctaggccagccagaatttgagcaacaacgggaattgcccccctggggagaaatattttctgaatattgtttattcatccgtcccagcaatgtcactgaagaagaaagatttgtacaaagggtagtggactttttgcaaattcattgtcaccaatccatcgttgccgaacccttgtctgaagctcaaactttggagcaccgtcaggggcaaattcattactgccaacaacaacagaaaaatgataaaacccgtcgggtactggaaaaagcttttggggaagcttgggcggaacggtatatgagccaagtcttatttgatgttatccaataataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R1051_sequence/1
type: Sequence
persistent identity: BBa R1051 sequence
display id: BBa_R1051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: taacaccgtgcgtgttgacaattttacctctggcggtgatactggttgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I732100_sequence/1
type: Sequence
persistent identity: BBa I732100 sequence
display id: BBa_I732100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K081016_sequence/1
type: Sequence
persistent identity: BBa K081016 sequence
display id: BBa_K081016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaatactagatgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctctactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K395601_sequence/1
type: Sequence
persistent identity: BBa K395601 sequence
display id: BBa_K395601_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K395162_sequence/1
type: Sequence
persistent identity: BBa K395162 sequence
display id: BBa_K395162_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagagctaaggaagctaaaatggagaaaaaaatcacgggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaacgctcacccggagtttcgtatggccatgaaagacggtgagctggtgatctgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcgtccctctggagtgaataccacgacgatttccggcagtttctccacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgttttttgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgatccaggttcatcatgccgtttgtgatggcttccatgtcggccgcatgcttaatgaattacaacagtactgtgatgagtggcagggcggggcgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0061_sequence/1
type: Sequence
persistent identity: BBa R0061 sequence
display id: BBa_R0061_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacacctgtaggatcgtacaggtataat
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K395705_sequence/1
type: Sequence
persistent identity: BBa K395705 sequence
display id: BBa_K395705_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggaggtactagatgactgctgctgttgctgagcctagaatcgttcctagacagacttggatcggacttactcttgctggaatgatcgttgctggatggggatctcttcacgtttacggagtttacttccacagatggggaacttcttctcttgttatcgttcctgctatcgttgctgttcagacttggctttctgttggacttttcatcgttgctcatgatgctatgcacggatctcttgctcctggaagacctagacttaacgctgctgttggaagacttactcttggactttacgctggattcagattcgatagacttaagactgctcaccacgctcaccacgctgctcctggaactgctgatgatcctgatttctacgctcctgctcctagagctttccttccttggttccttaacttcttcagaacttacttcggatggagagagatggctgttcttactgctcttgttcttatcgctcttttcggacttggagctagacctgctaaccttcttactttctgggctgctcctgctcttctttctgctcttcagcttttcactttcggaacttggcttcctcacagacacactgatcagcctttcgctgatgctcaccacgctagatcttctggatacggacctgttctttctcttcttacttgcttccacttcggaagacaccacgagcaccaccttactccttggagaccttggtggagactttggagaggagagtcttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K395603_sequence/1
type: Sequence
persistent identity: BBa K395603 sequence
display id: BBa_K395603_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aactttaagaaggagatatacatatgcaccatcatcatcatcattcttctggtctggtgccacgcggttctggtatgaaagaaaccgctgctgctaaattcgaacgccagcacatggacagcccagatctgggtaccggtggtggctccggtattgagggtcgcatgatgtccttttccgtactgcaagtgaaacgtctgcaaccggaactgattacaccggcaaaatcaacccctcaggaaaccaaatttctgagcgacatcgatgatcaagaatctctgcgcgttcagattccgattatcatgtgttataaagacaacccgagcctgaataaaaaccgtaacccggtcaaagccattcgtgaagcactgtcacgtgccctggtctattattatccactggccggtcgtctgcgtgaaggtccaaaccgtaaactggtggtcgattgtaacggcgaaggcatcctgtttgtggaagcttccgccgatgttacgctggaacagctgggggacaaaatcctgcctccttgtcctctgctggaggagtttctgtataacttcccgggttctgatggcattatcgactgcccactgctgctgattcaagtgacctgtctgacttgtggagggttcatcctggctctgcgcctgaaccatacaatgtgtgatgccgccggtctgctgctgtttctgaccgctattgccgaaatggcccgtggcgctcatgcccctagtattctgcctgtctgggagcgtgaactgctgtttgctcgtgatcctcctcgtatcacctgtgcccaccatgaatatgaggacgtgattggccactcagatgggtcttatgccagctcaaatcagagtaatatggtccaacgctcgttctattttggcgccaaagagatgcgcgtactgcgtaaacaaattccgccacacctgattagcacttgtagcaccttcgatctgatcaccgcttgtctgtggaaatgtcgtaccctggcactgaacattaacccgaaagaggccgttcgtgtttcgtgtattgtgaatgctcgtggtaaacacaacaacgttcgcctgcctctgggttattatggtaacgcctttgcctttccggcagcaatttcaaaagccgaacctctgtgtaaaaacccgctgggatatgctctggaactggtaaaaaaagcgaaagcgactatgaacgaagagtatctgcgttctgttgctgatctgctggtactgcgcggtcgccctcagtatagtagtaccggctcctatctgatcgttagcgacaacacccgtgtaggcttcggagatgtgaattttggctggggtcagcctgtttttgctggaccggttaaagcactggatctgatctcgttttatgtccaacacaaaaacaacaccgaggatggcattctggttccgatgtgtctgcctagtagcgctatggaacgctttcagcaggaactggagcgtattacccaggaaccaaaagaggacatctgtaacaatctgcgctcaacgtcccagtaaggctctaactctcctctggccatggcgatatcggatccaaattcgagctccgtcgacaagcttgcggccgcactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgatactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0082_sequence/1
type: Sequence
persistent identity: BBa R0082 sequence
display id: BBa_R0082_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcccttgcatttacattttgaaacatctatagcgataaatgaaacatcttaaaagttttagtatcatattcgtgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0051_sequence/1
type: Sequence
persistent identity: BBa C0051 sequence
display id: BBa_C0051_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagcacaaaaaagaaaccattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatcccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaatgcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatctacgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggcagggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagtgattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaatgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttaccttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaatgagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K274200_sequence/1
type: Sequence
persistent identity: BBa K274200 sequence
display id: BBa_K274200_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaggtactagatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcaaggaggtactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I0462_sequence/1
type: Sequence
persistent identity: BBa I0462 sequence
display id: BBa_I0462_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23107_sequence/1
type: Sequence
persistent identity: BBa J23107 sequence
display id: BBa_J23107_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tttacggctagctcagccctaggtattatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0011_sequence/1
type: Sequence
persistent identity: BBa R0011 sequence
display id: BBa_R0011_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I15010_sequence/1
type: Sequence
persistent identity: BBa I15010 sequence
display id: BBa_I15010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccaccaccgtacaactcagcgaccaatccctccgtcagctagaaaccctcgccatccacaccgcccacctgattcagccccacggtttagtggtggtcctgcaagaaccagacctcaccatcagccaaattagcgccaactgcaccggcattttagggcgatcgccagaggatttgttgggcagaaccctaggggaagtgtttgatagctttcagattgatcccatccagagtcgcctaacggccggacaaatcagcagcctcaaccccagtaaactttgggcgcgggtcatgggggacgactttgtcatttttgacggggtttttcatcgcaacagtgacggtttattggtatgtgaactcgagccagcctacacttccgataatctgcccttcctcggtttttatcacatggccaacgctgccctgaatcggttgcgccaacaagctaatctacgggatttctacgatgttattgtcgaagaagtccgccgtatgactggctttgaccgggtgatgctataccgctttgatgaaaataaccacggtgatgtcattgccgaagataaacgggatgatatggaaccctatttgggcctgcactatcccgaatcggatattccccaacccgcccgtcggctatttatccacaaccccattcgagtaattcccgatgtttatggtgtggcggtgcccctgaccccagcggttaaccccagcaccaaccgagcggtggatttaacagaatccattctgcgcagtgcgtaccattgccacttgacctatctgaaaaatatgggggtaggagcgtctttaaccatttccctaattaaggacggccatctctgggggctcattgcctgccaccatcaaacccccaaagtaattccctttgaactgcgtaaagcctgcgaattttttggtcgggtggtgtttagcaacatttccgcccaggaagatacggaaaccttcgattaccgggtgcagctggcggagcatgaagcggttttattggacaaaatgaccacggcggcggattttgtcgaaggattaactaatcatcccgatcgcctgttgggattaacgggctcccagggggcggccatttgctttggggaaaaattgattttagtaggggaaaccccggacgagaaagcagtgcaatatttactgcaatggttggagaatcgggaagtgcaagacgttttcttcacctcttccctctcacaaatttatcctgatgcagtgaattttaaatccgtggccagtggcttattggccattcccattgcccgtcacaactttttgctctggtttcgccctgaagtgttgcaaacggttaattggggcggtgacccaaatcatgcttacgaagctacccaggaagacggtaaaatcgagctccatccccgccaatcctttgacctctggaaagaaattgtccgactccaatctttgccctggcaatcggtggaaatccaaagtgccctggccctgaaaaaggcgatcgtcaacctcattttgcgccaggcagaagaattgcatatggcggctggtgttaagcaactggcggatgaccgcacgctgctgatggcgggggtaagtcacgacttgcgcacgccgctgacgcgtattcgcctggcgactgagatgatgagcgagcaggatggctatctggcagaatcgatcaataaagatatcgaagagtgcaacgccatcattgagcagtttatcgactacctgcgcaccgggcaggagatgccgatggaaatggcggatcttaatgcagtactcggtgaggtgattgctgccgaaagtggctatgagcgggaaattgaaaccgcgctttaccccggcagcattgaagtgaaaatgcacccgctgtcgatcaaacgcgcggtggcgaatatggtggtcaacgccgcccgttatggcaatggctgggtcaaagtcagcagcggaacggagccgaatcgcgcctggttccaggtggaaga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_Q04400_sequence/1
type: Sequence
persistent identity: BBa Q04400 sequence
display id: BBa_Q04400_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K395407_sequence/1
type: Sequence
persistent identity: BBa K395407 sequence
display id: BBa_K395407_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atttacattttgaaacatctgaccgttggattattctgcatttttggggagaatggacttactagagaaagaggagaaatactagatgaagccggtgaccctgtatgacgtggcggaatacgcgggcgtgagctaccaaacggtgagccgtgttgttaatcaagcgtctcatgtgagcgccaagacccgcgagaaggttgaggccgccatggccgaattaaactatatcccgaatcgtgttgcgcagcagttagccggtaagcaaagcctgttaatcggtgtggcgacgagcagcttagcgtcacatgccccaagccagatcgtggccgccatcaagagccgtgcggaccagttaggcgcgtctgttgttgttagaatggtggagcgctctggtgtggaggcgtgcaaggccgccgttcataacctgctggcccagcgtgtgagcggcttaattatcaattacccattagacgatcaagacgcgatcgcggttgaggcggcgtgtaccaacgtgccagccctgttcctggacgtgagcgatcaaaccccgattaatagcatcatctttagccacgagaatggcacccgcttaggtgttgaacacttagtggccctgggccatcaacagattgccctactggccggtccgctgagcagcgtgagcgcccgcttacgcttagcgggttggcacaagtacctgacccgtaaccagatccaaccaattgccgagcgcgagggtgattggagcgcgatgagcggcttccagcagacgatgcagatgttaaacgaaggtattgtgccgaccgccatgttagtggcgaatgaccaaatggccttaggtgcgatgcgtgcgatcacggaaagcggcttacgtgtgggcgccgacattagcgtggttggctatgatgacacggaggattctagctgctacattccaccaagcacgacgattaagcaagacttccgtttattaggccagacgtctgttgatcgtctgttacagctgagccaaggtcaagccgttaaaggtaaccaattactgccggtgagcttagttaagcgcaagacgacgttagccccgaacacccagacggcgagcccgcgtgccctggcggacagcctgatgcaattagcgcgccaagtgagccgcttagagtctggccaatgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0030_sequence/1
type: Sequence
persistent identity: BBa B0030 sequence
display id: BBa_B0030_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: attaaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0062_sequence/1
type: Sequence
persistent identity: BBa R0062 sequence
display id: BBa_R0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742156_sequence/1
type: Sequence
persistent identity: BBa I742156 sequence
display id: BBa_I742156_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctctaccggagaaatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742157_sequence/1
type: Sequence
persistent identity: BBa I742157 sequence
display id: BBa_I742157_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgttgtggatttggaatgccctgatcgttttcgttaccgtgattggcatggaagtgattgctgcactggcacacaaatacatcatgcacggctggggttggggatggcatctttcacatcatgaaccgcgtaaaggtgcgtttgaagttaacgatctttatgccgtggtttttgctgcattatcgatcctgctgatttatctgggcagtacaggaatgtggccgctccagtggattggcgcaggtatgacggcgtatggattactctattttatggtgcacgacgggctggtgcatcaacgttggccattccgctatattccacgcaagggctacctcaaacggttgtatatggcgcaccgtatgcatcacgccgtcaggggcaaagaaggttgtgtttcttttggcttcctctatgcgccgcccctgtcaaaacttcaggcgacgctccgggaaagacatggcgctagagcgggcgctgccagagatgcgcagggcggggaggatgagcccgcatccgggaagtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15001_sequence/1
type: Sequence
persistent identity: BBa J15001 sequence
display id: BBa_J15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742151_sequence/1
type: Sequence
persistent identity: BBa I742151 sequence
display id: BBa_I742151_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0078_sequence/1
type: Sequence
persistent identity: BBa C0078 sequence
display id: BBa_C0078_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatcgttcagatcggtcgtcgtgaagagttcgacaaaaaactgctgggtgaaatgcacaaactgcgtgctcaggttttcaaagaacgtaaaggttgggacgtttccgttatcgacgaaatggaaatcgacggttacgacgctctgtccccgtactacatgctgatccaggaagacaccccggaagctcaggttttcggttgctggcgtatcttcgacaccaccggtccgtacatgctgaaaaacaccttcccggaactgctgcacggtaaagaagctccgtgctccccgcacatctgggaactgtcccgtttcgctatcaactccggtcagaaaggttccctgggtttctccgactgcaccctggaagctatgcgtgctctggctcgttactccttgcagaacgacatccagaccctggttaccgttaccaccgttggtgttgaaaaaatgatgatccgtgctggtctggacgtttcccgtttcggtccgcacctgaaaatcggtatcgaacgtgctgttgctctgcgtatcgaactgaacgctaaaacccagatcgctctgtacggtggtgttctggttgaacagcgtctggctgtttccgctgctaacgacgaaaactacgctctggttgcttaataactctgatagtgctagtgtagatctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118002_sequence/1
type: Sequence
persistent identity: BBa K118002 sequence
display id: BBa_K118002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118012_sequence/1
type: Sequence
persistent identity: BBa K118012 sequence
display id: BBa_K118012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: myers
owned by: james
elements: gagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K395160_sequence/1
type: Sequence
persistent identity: BBa K395160 sequence
display id: BBa_K395160_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agctaaggaagctaaaatggagaaaaaaatcacgggatataccaccgttgatatatcccaatggcatcgtaaagaacattttgaggcatttcagtcagttgctcaatgtacctataaccagaccgttcagctggatattacggcctttttaaagaccgtaaagaaaaataagcacaagttttatccggcctttattcacattcttgcccgcctgatgaacgctcacccggagtttcgtatggccatgaaagacggtgagctggtgatctgggatagtgttcacccttgttacaccgttttccatgagcaaactgaaacgttttcgtccctctggagtgaataccacgacgatttccggcagtttctccacatatattcgcaagatgtggcgtgttacggtgaaaacctggcctatttccctaaagggtttattgagaatatgttttttgtctcagccaatccctgggtgagtttcaccagttttgatttaaacgtggccaatatggacaacttcttcgcccccgttttcacgatgggcaaatattatacgcaaggcgacaaggtgctgatgccgctggcgatccaggttcatcatgccgtttgtgatggcttccatgtcggccgcatgcttaatgaattacaacagtactgtgatgagtggcagggcggggcgtaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K395700_sequence/1
type: Sequence
persistent identity: BBa K395700 sequence
display id: BBa_K395700_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgactgctgctgttgctgagcctagaatcgttcctagacagacttggatcggacttactcttgctggaatgatcgttgctggatggggatctcttcacgtttacggagtttacttccacagatggggaacttcttctcttgttatcgttcctgctatcgttgctgttcagacttggctttctgttggacttttcatcgttgctcatgatgctatgcacggatctcttgctcctggaagacctagacttaacgctgctgttggaagacttactcttggactttacgctggattcagattcgatagacttaagactgctcaccacgctcaccacgctgctcctggaactgctgatgatcctgatttctacgctcctgctcctagagctttccttccttggttccttaacttcttcagaacttacttcggatggagagagatggctgttcttactgctcttgttcttatcgctcttttcggacttggagctagacctgctaaccttcttactttctgggctgctcctgctcttctttctgctcttcagcttttcactttcggaacttggcttcctcacagacacactgatcagcctttcgctgatgctcaccacgctagatcttctggatacggacctgttctttctcttcttacttgcttccacttcggaagacaccacgagcaccaccttactccttggagaccttggtggagactttggagaggagagtcttga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118003_sequence/1
type: Sequence
persistent identity: BBa K118003 sequence
display id: BBa_K118003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K395604_sequence/1
type: Sequence
persistent identity: BBa K395604 sequence
display id: BBa_K395604_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aactttaagaaggagatatacatatgcaccatcatcatcatcattcttctggtctggtgccacgcggttctggtatgaaagaaaccgctgctgctaaattcgaacgccagcacatggacagcccagatctgggtaccggtggtggctccggtattgagggtcgcatgatgtccttttccgtactgcaagtgaaacgtctgcaaccggaactgattacaccggcaaaatcaacccctcaggaaaccaaatttctgagcgacatcgatgatcaagaatctctgcgcgttcagattccgattatcatgtgttataaagacaacccgagcctgaataaaaaccgtaacccggtcaaagccattcgtgaagcactgtcacgtgccctggtctattattatccactggccggtcgtctgcgtgaaggtccaaaccgtaaactggtggtcgattgtaacggcgaaggcatcctgtttgtggaagcttccgccgatgttacgctggaacagctgggggacaaaatcctgcctccttgtcctctgctggaggagtttctgtataacttcccgggttctgatggcattatcgactgcccactgctgctgattcaagtgacctgtctgacttgtggagggttcatcctggctctgcgcctgaaccatacaatgtgtgatgccgccggtctgctgctgtttctgaccgctattgccgaaatggcccgtggcgctcatgcccctagtattctgcctgtctgggagcgtgaactgctgtttgctcgtgatcctcctcgtatcacctgtgcccaccatgaatatgaggacgtgattggccactcagatgggtcttatgccagctcaaatcagagtaatatggtccaacgctcgttctattttggcgccaaagagatgcgcgtactgcgtaaacaaattccgccacacctgattagcacttgtagcaccttcgatctgatcaccgcttgtctgtggaaatgtcgtaccctggcactgaacattaacccgaaagaggccgttcgtgtttcgtgtattgtgaatgctcgtggtaaacacaacaacgttcgcctgcctctgggttattatggtaacgcctttgcctttccggcagcaatttcaaaagccgaacctctgtgtaaaaacccgctgggatatgctctggaactggtaaaaaaagcgaaagcgactatgaacgaagagtatctgcgttctgttgctgatctgctggtactgcgcggtcgccctcagtatagtagtaccggctcctatctgatcgttagcgacaacacccgtgtaggcttcggagatgtgaattttggctggggtcagcctgtttttgctggaccggttaaagcactggatctgatctcgttttatgtccaacacaaaaacaacaccgaggatggcattctggttccgatgtgtctgcctagtagcgctatggaacgctttcagcaggaactggagcgtattacccaggaaccaaaagaggacatctgtaacaatctgcgctcaacgtcccagtaaggctctaactctcctctggccatggcgatatcggatccaaattcgagctccgtcgacaagcttgcggccgcactcgagcaccaccaccaccaccactgagatccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118008_sequence/1
type: Sequence
persistent identity: BBa K118008 sequence
display id: BBa_K118008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaaccgcattatgatctgattctcgtgggggctggactcgcgaatggccttatcgccctgcgtcttcagcagcagcaacctgatatgcgtattttgcttatcgacgccgcaccccaggcgggcgggaatcatacgtggtcatttcaccacgatgatttgactgagagccaacatcgttggatagctccgctggtggttcatcactggcccgactatcaggtacgctttcccacacgccgtcgtaagctgaacagcggctacttttgtattacttctcagcgtttcgctgaggttttacagcgacagtttggcccgcacttgtggatggataccgcggtcgcagaggttaatgcggaatctgttcggttgaaaaagggtcaggttatcggtgcccgcgcggtgattgacgggcggggttatgcggcaaattcagcactgagcgtgggcttccaggcgtttattggccaggaatggcgattgagccacccgcatggtttatcgtctcccattatcatggatgccacggtcgatcagcaaaatggttatcgcttcgtgtacagcctgccgctctcgccgaccagattgttaattgaagacacgcactatattgataatgcgacattagatcctgaatgcgcgcggcaaaatatttgcgactatgccgcgcaacagggttggcagcttcagacactgctgcgagaagaacagggcgccttacccattactctgtcgggcaatgccgacgcattctggcagcagcgccccctggcctgtagtggattacgtgccggtctgttccatcctaccaccggctattcactgccgctggcggttgccgtggccgaccgcctgagtgcacttgatgtctttacgtcggcctcaattcaccatgccattacgcattttgcccgcgagcgctggcagcagcagggctttttccgcatgctgaatcgcatgctgtttttagccggacccgccgattcacgctggcgggttatgcagcgtttttatggtttacctgaagatttaattgcccgtttttatgcgggaaaactcacgctgaccgatcggctacgtattctgagcggcaagccgcctgttccggtattagcagcattgcaagccattatgacgactcatcgttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K395303_sequence/1
type: Sequence
persistent identity: BBa K395303 sequence
display id: BBa_K395303_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atttacattttgaaacatctgaccgttggattattctgcatttttggggagaatggact
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K082005_sequence/1
type: Sequence
persistent identity: BBa K082005 sequence
display id: BBa_K082005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaagccggtgaccctgtatgacgtggcggaatacgcgggcgtgagctaccaaacggtgagccgtgttgttaatcaagcgtctcatgtgagcgccaagacccgcgagaaggttgaggccgccatggccgaattaaactatatcccgaatcgtgttgcgcagcagttagccggtaagcaaagcctgttaatcggtgtggcgacgagcagcttagcgtcacatgccccaagccagatcgtggccgccatcaagagccgtgcggaccagttaggcgcgtctgttgttgttagaatggtggagcgctctggtgtggaggcgtgcaaggccgccgttcataacctgctggcccagcgtgtgagcggcttaattatcaattacccattagacgatcaagacgcgatcgcggttgaggcggcgtgtaccaacgtgccagccctgttcctggacgtgagcgatcaaaccccgattaatagcatcatctttagccacgagaatggcacccgcttaggtgttgaacacttagtggccctgggccatcaacagattgccctactggccggtccgctgagcagcgtgagcgcccgcttacgcttagcgggttggcacaagtacctgacccgtaaccagatccaaccaattgccgagcgcgagggtgattggagcgcgatgagcggcttccagcagacgatgcagatgttaaacgaaggtattgtgccgaccgccatgttagtggcgaatgaccaaatggccttaggtgcgatgcgtgcgatcacggaaagcggcttacgtgtgggcgccgacattagcgtggttggctatgatgacacggaggattctagctgctacattccaccaagcacgacgattaagcaagacttccgtttattaggccagacgtctgttgatcgtctgttacagctgagccaaggtcaagccgttaaaggtaaccaattactgccggtgagcttagttaagcgcaagacgacgttagccccgaacacccagacggcgagcccgcgtgccctggcggacagcctgatgcaattagcgcgccaagtgagccgcttagagtctggccaatga
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z