LOCUS BBa_K395999 19005 bp DNA linear 22-Dec-2024 DEFINITION Wolfcoli system ACCESSION BBa_K395999 VERSION BBa_K395999.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23107 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 misc_feature 788..812 /label=Help:Barcodes CDS 62..812 /label=BBa_I15008 misc_feature 825..828 /label=conserved RBS 821..832 /label=BBa_B0034 CDS 839..1588 /label=PcyA misc_feature 1589..1613 /label=Help:Barcodes CDS 839..1613 /label=BBa_I15009 stem_loop 1633..1676 /label=stem_loop misc_feature 1622..1701 /label=BBa_B0010 terminator 1622..1701 /label=BBa_B0010 stem_loop 1717..1736 /label=T7 TE misc_feature 1743..1743 /label=stop polyA_site 1737..1750 /label=polya misc_feature 1710..1750 /label=BBa_B0012 terminator 1710..1750 /label=BBa_B0012 terminator 1622..1750 /label=BBa_B0015 promoter 1759..1793 /label=BBa_J23107 misc_feature 1806..1809 /label=conserved RBS 1802..1813 /label=BBa_B0034 CDS 1820..4057 /label=Cph8 misc_feature 4058..4082 /label=Help:Barcodes CDS 1820..4082 /label=BBa_I15010 stem_loop 4102..4145 /label=stem_loop misc_feature 4091..4170 /label=BBa_B0010 terminator 4091..4170 /label=BBa_B0010 stem_loop 4186..4205 /label=T7 TE misc_feature 4212..4212 /label=stop polyA_site 4206..4219 /label=polya misc_feature 4179..4219 /label=BBa_B0012 terminator 4179..4219 /label=BBa_B0012 terminator 4091..4219 /label=BBa_B0015 misc_feature 4228..4247 /label=Binding site1 promoter 4253..4258 /label=-35 promoter 4276..4281 /label=-10 promoter 4228..4286 /label=BBa_K395303 misc_feature 4299..4302 /label=conserved RBS 4295..4306 /label=BBa_B0034 misc_feature 4313..5395 /label=BBa_K082005 CDS 4313..5395 /label=BBa_K082005 stem_loop 5415..5458 /label=stem_loop misc_feature 5404..5483 /label=BBa_B0010 terminator 5404..5483 /label=BBa_B0010 stem_loop 5499..5518 /label=T7 TE misc_feature 5525..5525 /label=stop polyA_site 5519..5532 /label=polya misc_feature 5492..5532 /label=BBa_B0012 terminator 5492..5532 /label=BBa_B0012 misc_feature 4228..5532 /label=BBa_K395407 misc_feature 5553..5570 /label=C1 OmpR misc_feature 5574..5591 /label=C2 OmpR misc_feature 5594..5611 /label=C3 OmpR promoter 5615..5620 /label=-35 promoter 5638..5643 /label=-10 promoter 5541..5648 /label=BBa_R0082 misc_feature 5661..5664 /label=conserved RBS 5657..5668 /label=BBa_B0034 CDS 5678..6385 /label=cI lambda misc_feature 6386..6418 /label=LVA misc_feature 6425..6449 /label=Help:Barcodes CDS 5675..6449 /label=BBa_C0051 stem_loop 6469..6512 /label=stem_loop misc_feature 6458..6537 /label=BBa_B0010 terminator 6458..6537 /label=BBa_B0010 stem_loop 6553..6572 /label=T7 TE misc_feature 6579..6579 /label=stop polyA_site 6573..6586 /label=polya misc_feature 6546..6586 /label=BBa_B0012 terminator 6546..6586 /label=BBa_B0012 terminator 6458..6586 /label=BBa_B0015 promoter 6609..6614 /label=-35 promoter 6619..6635 /label=OR1 promoter 6595..6611 /label=OR2 promoter 6632..6637 /label=-10 misc_feature 6595..6643 /label=BBa_R1051 promoter 6595..6643 /label=BBa_R1051 misc_feature 6656..6659 /label=conserved RBS 6652..6663 /label=BBa_B0034 CDS 6670..7755 /label=BBa_I732100 stem_loop 7775..7818 /label=stem_loop misc_feature 7764..7843 /label=BBa_B0010 terminator 7764..7843 /label=BBa_B0010 stem_loop 7859..7878 /label=T7 TE misc_feature 7885..7885 /label=stop polyA_site 7879..7892 /label=polya misc_feature 7852..7892 /label=BBa_B0012 terminator 7852..7892 /label=BBa_B0012 terminator 7764..7892 /label=BBa_B0015 misc_feature 7903..7919 /label=lac O1 promoter 7920..7925 /label=-35 misc_feature 7926..7942 /label=lac O1 promoter 7943..7948 /label=-10 misc_feature 7901..7954 /label=BBa_R0011 promoter 7901..7954 /label=BBa_R0011 misc_feature 7968..7971 /label=conserved RBS 7964..7975 /label=BBa_B0034 CDS 7985..8601 /label=tetR misc_feature 8602..8635 /label=SsrA misc_feature 8642..8666 /label=Help:Barcodes CDS 7982..8641 /label=BBa_C0040 stem_loop 8686..8729 /label=stem_loop misc_feature 8675..8754 /label=BBa_B0010 terminator 8675..8754 /label=BBa_B0010 stem_loop 8770..8789 /label=T7 TE misc_feature 8796..8796 /label=stop polyA_site 8790..8803 /label=polya misc_feature 8763..8803 /label=BBa_B0012 terminator 8763..8803 /label=BBa_B0012 misc_feature 8812..8830 /label=TetR 1 misc_feature 8812..8865 /label=BBa_R0040 promoter 8831..8836 /label=-35 misc_feature 8837..8855 /label=TetR 2 promoter 8854..8859 /label=-10 promoter 8812..8865 /label=BBa_R0040 misc_feature 7964..8865 /label=BBa_Q04400 misc_feature 8878..8881 /label=conserved RBS 8874..8885 /label=BBa_B0034 CDS 8892..9608 /label=lasR misc_feature 9609..9641 /label=LVA misc_feature 9371..9371 /label=G misc_feature 9648..9672 /label=Help:Barcodes CDS 8892..9647 /label=BBa_C0079 misc_feature 8874..9672 /label=BBa_S03156 stem_loop 9692..9735 /label=stem_loop misc_feature 9681..9760 /label=BBa_B0010 terminator 9681..9760 /label=BBa_B0010 stem_loop 9776..9795 /label=T7 TE misc_feature 9802..9802 /label=stop polyA_site 9796..9809 /label=polya misc_feature 9769..9809 /label=BBa_B0012 terminator 9769..9809 /label=BBa_B0012 terminator 9681..9809 /label=BBa_B0015 misc_feature 8874..9809 /label=BBa_S03882 promoter 9818..9847 /label=BBa_K395025 misc_feature 9863..9874 /label=B0034 RBS CDS 9881..10462 /label=LuxI misc_feature 10499..10627 /label=B0015 Terminator misc_feature 10463..10498 /label=barcode misc_feature 9856..10627 /label=BBa_K092400 misc_feature 10636..10655 /label=LuxR/HSL promoter 10655..10660 /label=-35 promoter 10677..10682 /label=-10 misc_feature 10688..10688 /label=start misc_feature 10636..10690 /label=BBa_R0062 promoter 10636..10690 /label=BBa_R0062 RBS 10704..10707 /label=RBS misc_feature 10715..11375 /label=CmR misc_feature 10699..11377 /label=BBa_K395160 misc_feature 10636..11377 /label=BBa_K395162 RBS 11386..11389 /label=BBa_K118012 CDS 11396..12307 /label=BBa_I742151 misc_feature 12316..12318 /label=SacI RBS 12319..12325 /label=rbs RBS 12316..12325 /label=BBa_J15001 CDS 12332..13261 /label=crtB coding sequence CDS 12332..13264 /label=BBa_K118002 misc_feature 13273..13275 /label=SacI RBS 13276..13282 /label=rbs RBS 13273..13282 /label=BBa_J15001 misc_feature 13766..13771 /label=PstI site removed misc_feature 14177..14182 /label=PstI site removed CDS 13289..14767 /label=crtI coding sequence CDS 13289..14770 /label=BBa_K118003 RBS 14779..14782 /label=BBa_K118012 CDS 14789..15937 /label=crtY coding sequence CDS 14789..15940 /label=BBa_K118008 misc_feature 11386..15940 /label=BBa_K274200 RBS 15949..15964 /label=BBa_I742156 CDS 15965..16492 /label=crtZ CDS 15965..16495 /label=BBa_I742157 misc_feature 15949..16495 /label=BBa_I742158 misc_feature 16504..16506 /label=SacI RBS 16507..16513 /label=rbs RBS 16504..16513 /label=BBa_J15001 CDS 16520..17254 /label=BBa_K395700 misc_feature 16504..17254 /label=BBa_K395705 RBS 17272..17276 /label=rbs misc_feature 17286..17286 /label=start misc_feature 17289..17306 /label=his-tag misc_feature 17340..17373 /label=s-tag misc_feature 17415..17426 /label=factor-Xa CDS 17427..18794 /label=MpAAT1 misc_feature 18794..18794 /label=stop misc_feature 18873..18890 /label=His-tag misc_feature 17263..18949 /label=BBa_K395604 terminator 18958..19005 /label=BBa_K395601 ORIGIN 1 tttacggcta gctcagccct aggtattatg ctagctacta gagaaagagg agaaatacta 61 gatgagtgtc aacttagctt cccagttgcg ggaagggacg aaaaaatccc actccatggc 121 ggagaacgtc ggctttgtca aatgcttcct caagggcgtt gtcgagaaaa attcctaccg 181 taagctggtt ggcaatctct actttgtcta cagtgccatg gaagaggaaa tggcaaaatt 241 taaggaccat cccatcctca gccacattta cttccccgaa ctcaaccgca aacaaagcct 301 agagcaagac ctgcaattct attacggctc caactggcgg caagaagtga aaatttctgc 361 cgctggccaa gcctatgtgg accgagtccg gcaagtggcc gctacggccc ctgaattgtt 421 ggtggcccat tcctacaccc gttacctggg ggatctttcc ggcggtcaaa ttctcaagaa 481 aattgcccaa aatgccatga atctccacga tggtggcaca gctttctatg aatttgccga 541 cattgatgac gaaaaggctt ttaaaaatac ctaccgtcaa gctatgaatg atctgcccat 601 tgaccaagcc accgccgaac ggattgtgga tgaagccaat gacgcctttg ccatgaacat 661 gaaaatgttc aacgaacttg aaggcaacct gatcaaggcg atcggcatta tggtgttcaa 721 cagcctcacc cgtcgccgca gtcaaggcag caccgaagtt ggcctcgcca cctccgaagg 781 ctaataacgc tgatagtgct agtgtagatc gctactagag aaagaggaga aatactagat 841 ggccgtcact gatttaagtt tgaccaattc ttccctgatg cctacgttga acccgatgat 901 tcaacagttg gccctggcga tcgccgctag ttggcaaagt ttacccctca agccctatca 961 attgccggag gatttgggct acgtagaagg ccgcctggaa ggggaaaagt tagtgattga 1021 aaatcggtgc taccaaacgc cccagtttcg caaaatgcat ttggagttgg ccaaggtggg 1081 caaagggttg gatattctcc actgtgtaat gtttcctgag cctttatacg gtctaccttt 1141 gtttggctgt gacattgtgg ccggccccgg tggagtaagt gcggctattg cggatctatc 1201 ccccacccaa agcgatcgcc aattgcccgc agcgtaccaa aaatcattgg cagagctagg 1261 ccagccagaa tttgagcaac aacgggaatt gcccccctgg ggagaaatat tttctgaata 1321 ttgtttattc atccgtccca gcaatgtcac tgaagaagaa agatttgtac aaagggtagt 1381 ggactttttg caaattcatt gtcaccaatc catcgttgcc gaacccttgt ctgaagctca 1441 aactttggag caccgtcagg ggcaaattca ttactgccaa caacaacaga aaaatgataa 1501 aacccgtcgg gtactggaaa aagcttttgg ggaagcttgg gcggaacggt atatgagcca 1561 agtcttattt gatgttatcc aataataact ctgatagtgc tagtgtagat ctctactaga 1621 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt 1681 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc 1741 tgcgtttata tactagagtt tacggctagc tcagccctag gtattatgct agctactaga 1801 gaaagaggag aaatactaga tggccaccac cgtacaactc agcgaccaat ccctccgtca 1861 gctagaaacc ctcgccatcc acaccgccca cctgattcag ccccacggtt tagtggtggt 1921 cctgcaagaa ccagacctca ccatcagcca aattagcgcc aactgcaccg gcattttagg 1981 gcgatcgcca gaggatttgt tgggcagaac cctaggggaa gtgtttgata gctttcagat 2041 tgatcccatc cagagtcgcc taacggccgg acaaatcagc agcctcaacc ccagtaaact 2101 ttgggcgcgg gtcatggggg acgactttgt catttttgac ggggtttttc atcgcaacag 2161 tgacggttta ttggtatgtg aactcgagcc agcctacact tccgataatc tgcccttcct 2221 cggtttttat cacatggcca acgctgccct gaatcggttg cgccaacaag ctaatctacg 2281 ggatttctac gatgttattg tcgaagaagt ccgccgtatg actggctttg accgggtgat 2341 gctataccgc tttgatgaaa ataaccacgg tgatgtcatt gccgaagata aacgggatga 2401 tatggaaccc tatttgggcc tgcactatcc cgaatcggat attccccaac ccgcccgtcg 2461 gctatttatc cacaacccca ttcgagtaat tcccgatgtt tatggtgtgg cggtgcccct 2521 gaccccagcg gttaacccca gcaccaaccg agcggtggat ttaacagaat ccattctgcg 2581 cagtgcgtac cattgccact tgacctatct gaaaaatatg ggggtaggag cgtctttaac 2641 catttcccta attaaggacg gccatctctg ggggctcatt gcctgccacc atcaaacccc 2701 caaagtaatt ccctttgaac tgcgtaaagc ctgcgaattt tttggtcggg tggtgtttag 2761 caacatttcc gcccaggaag atacggaaac cttcgattac cgggtgcagc tggcggagca 2821 tgaagcggtt ttattggaca aaatgaccac ggcggcggat tttgtcgaag gattaactaa 2881 tcatcccgat cgcctgttgg gattaacggg ctcccagggg gcggccattt gctttgggga 2941 aaaattgatt ttagtagggg aaaccccgga cgagaaagca gtgcaatatt tactgcaatg 3001 gttggagaat cgggaagtgc aagacgtttt cttcacctct tccctctcac aaatttatcc 3061 tgatgcagtg aattttaaat ccgtggccag tggcttattg gccattccca ttgcccgtca 3121 caactttttg ctctggtttc gccctgaagt gttgcaaacg gttaattggg gcggtgaccc 3181 aaatcatgct tacgaagcta cccaggaaga cggtaaaatc gagctccatc cccgccaatc 3241 ctttgacctc tggaaagaaa ttgtccgact ccaatctttg ccctggcaat cggtggaaat 3301 ccaaagtgcc ctggccctga aaaaggcgat cgtcaacctc attttgcgcc aggcagaaga 3361 attgcatatg gcggctggtg ttaagcaact ggcggatgac cgcacgctgc tgatggcggg 3421 ggtaagtcac gacttgcgca cgccgctgac gcgtattcgc ctggcgactg agatgatgag 3481 cgagcaggat ggctatctgg cagaatcgat caataaagat atcgaagagt gcaacgccat 3541 cattgagcag tttatcgact acctgcgcac cgggcaggag atgccgatgg aaatggcgga 3601 tcttaatgca gtactcggtg aggtgattgc tgccgaaagt ggctatgagc gggaaattga 3661 aaccgcgctt taccccggca gcattgaagt gaaaatgcac ccgctgtcga tcaaacgcgc 3721 ggtggcgaat atggtggtca acgccgcccg ttatggcaat ggctgggtca aagtcagcag 3781 cggaacggag ccgaatcgcg cctggttcca ggtggaagat gacggtccgg gaattgcgcc 3841 ggaacaacgt aagcacctgt tccagccgtt tgtccgcggc gacagtgcgc gcaccattag 3901 cggcacggga ttagggctgg caattgtgca gcgtatcgtg gataaccata acgggatgct 3961 ggagcttggc accagcgagc ggggcgggct ttccattcgc gcctggctgc cagtgccggt 4021 aacgcgggcg cagggcatga caaaagaagg gtaataactc tgatagtgct agtgtagatc 4081 tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt 4141 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt 4201 gggcctttct gcgtttatat actagagatt tacattttga aacatctgac cgttggatta 4261 ttctgcattt ttggggagaa tggacttact agagaaagag gagaaatact agatgaagcc 4321 ggtgaccctg tatgacgtgg cggaatacgc gggcgtgagc taccaaacgg tgagccgtgt 4381 tgttaatcaa gcgtctcatg tgagcgccaa gacccgcgag aaggttgagg ccgccatggc 4441 cgaattaaac tatatcccga atcgtgttgc gcagcagtta gccggtaagc aaagcctgtt 4501 aatcggtgtg gcgacgagca gcttagcgtc acatgcccca agccagatcg tggccgccat 4561 caagagccgt gcggaccagt taggcgcgtc tgttgttgtt agaatggtgg agcgctctgg 4621 tgtggaggcg tgcaaggccg ccgttcataa cctgctggcc cagcgtgtga gcggcttaat 4681 tatcaattac ccattagacg atcaagacgc gatcgcggtt gaggcggcgt gtaccaacgt 4741 gccagccctg ttcctggacg tgagcgatca aaccccgatt aatagcatca tctttagcca 4801 cgagaatggc acccgcttag gtgttgaaca cttagtggcc ctgggccatc aacagattgc 4861 cctactggcc ggtccgctga gcagcgtgag cgcccgctta cgcttagcgg gttggcacaa 4921 gtacctgacc cgtaaccaga tccaaccaat tgccgagcgc gagggtgatt ggagcgcgat 4981 gagcggcttc cagcagacga tgcagatgtt aaacgaaggt attgtgccga ccgccatgtt 5041 agtggcgaat gaccaaatgg ccttaggtgc gatgcgtgcg atcacggaaa gcggcttacg 5101 tgtgggcgcc gacattagcg tggttggcta tgatgacacg gaggattcta gctgctacat 5161 tccaccaagc acgacgatta agcaagactt ccgtttatta ggccagacgt ctgttgatcg 5221 tctgttacag ctgagccaag gtcaagccgt taaaggtaac caattactgc cggtgagctt 5281 agttaagcgc aagacgacgt tagccccgaa cacccagacg gcgagcccgc gtgccctggc 5341 ggacagcctg atgcaattag cgcgccaagt gagccgctta gagtctggcc aatgatacta 5401 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct 5461 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt 5521 tctgcgttta tatactagag tcccttgcat ttacattttg aaacatctat agcgataaat 5581 gaaacatctt aaaagtttta gtatcatatt cgtgttggat tattctgcat ttttggggag 5641 aatggactta ctagagaaag aggagaaata ctagatgagc acaaaaaaga aaccattaac 5701 acaagagcag cttgaggacg cacgtcgcct taaagcaatt tatgaaaaaa agaaaaatga 5761 acttggctta tcccaggaat ctgtcgcaga caagatgggg atggggcagt caggcgttgg 5821 tgctttattt aatggcatca atgcattaaa tgcttataac gccgcattgc ttgcaaaaat 5881 tctcaaagtt agcgttgaag aatttagccc ttcaatcgcc agagaaatct acgagatgta 5941 tgaagcggtt agtatgcagc cgtcacttag aagtgagtat gagtaccctg ttttttctca 6001 tgttcaggca gggatgttct cacctgagct tagaaccttt accaaaggtg atgcggagag 6061 atgggtaagc acaaccaaaa aagccagtga ttctgcattc tggcttgagg ttgaaggtaa 6121 ttccatgacc gcaccaacag gctccaagcc aagctttcct gacggaatgt taattctcgt 6181 tgaccctgag caggctgttg agccaggtga tttctgcata gccagacttg ggggtgatga 6241 gtttaccttc aagaaactga tcagggatag cggtcaggtg tttttacaac cactaaaccc 6301 acagtaccca atgatcccat gcaatgagag ttgttccgtt gtggggaaag ttatcgctag 6361 tcagtggcct gaagagacgt ttggcgctgc aaacgacgaa aactacgctt tagtagctta 6421 ataacgctga tagtgctagt gtagatcgct actagagcca ggcatcaaat aaaacgaaag 6481 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac 6541 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttatatact agagtaacac 6601 cgtgcgtgtt gacaatttta cctctggcgg tgatactggt tgctactaga gaaagaggag 6661 aaatactaga tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat 6721 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa 6781 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg 6841 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg 6901 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg 6961 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa 7021 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa 7081 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac 7141 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg 7201 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt 7261 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa 7321 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc 7381 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt 7441 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa 7501 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg 7561 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag 7621 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc 7681 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa 7741 agcgggcagt aataatacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 7801 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 7861 ctcaccttcg ggtgggcctt tctgcgttta tatactagag aattgtgagc ggataacaat 7921 tgacattgtg agcggataac aagatactga gcacatacta gagaaagagg agaaatacta 7981 gatgtccaga ttagataaaa gtaaagtgat taacagcgca ttagagctgc ttaatgaggt 8041 cggaatcgaa ggtttaacaa cccgtaaact cgcccagaag ctaggtgtag agcagcctac 8101 attgtattgg catgtaaaaa ataagcgggc tttgctcgac gccttagcca ttgagatgtt 8161 agataggcac catactcact tttgcccttt agaaggggaa agctggcaag attttttacg 8221 taataacgct aaaagtttta gatgtgcttt actaagtcat cgcgatggag caaaagtaca 8281 tttaggtaca cggcctacag aaaaacagta tgaaactctc gaaaatcaat tagccttttt 8341 atgccaacaa ggtttttcac tagagaatgc attatatgca ctcagcgctg tggggcattt 8401 tactttaggt tgcgtattgg aagatcaaga gcatcaagtc gctaaagaag aaagggaaac 8461 acctactact gatagtatgc cgccattatt acgacaagct atcgaattat ttgatcacca 8521 aggtgcagag ccagccttct tattcggcct tgaattgatc atatgcggat tagaaaaaca 8581 acttaaatgt gaaagtgggt ccgctgcaaa cgacgaaaac tacgctttag tagcttaata 8641 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct 8701 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag 8761 agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga gtccctatca 8821 gtgatagaga ttgacatccc tatcagtgat agagatactg agcactacta gagaaagagg 8881 agaaatacta gatggccttg gttgacggtt ttcttgagct ggaacgctca agtggaaaat 8941 tggagtggag cgccatcctc cagaagatgg cgagcgacct tggattctcg aagatcctgt 9001 tcggcctgtt gcctaaggac agccaggact acgagaacgc cttcatcgtc ggcaactacc 9061 cggccgcctg gcgcgagcat tacgaccggg ctggctacgc gcgggtcgac ccgacggtca 9121 gtcactgtac ccagagcgta ctgccgattt tctgggaacc gtccatctac cagacgcgaa 9181 agcagcacga gttcttcgag gaagcctcgg ccgccggcct ggtgtatggg ctgaccatgc 9241 cgctgcatgg tgctcgcggc gaactcggcg cgctgagcct cagcgtggaa gcggaaaacc 9301 gggccgaggc caaccgtttc atagagtcgg tcctgccgac cctgtggatg ctcaaggact 9361 acgcactgca aagcggtgcc ggactggcct tcgaacatcc ggtcagcaaa ccggtggttc 9421 tgaccagccg ggagaaggaa gtgttgcagt ggtgcgccat cggcaagacc agttgggaga 9481 tatcggttat ctgcaactgc tcggaagcca atgtgaactt ccatatggga aatattcggc 9541 ggaagttcgg tgtgacctcc cgccgcgtag cggccattat ggccgttaat ttgggtctta 9601 ttactctcgc tgcaaacgac gaaaactacg ctttagtagc ttaataacac tgatagtgct 9661 agtgtagatc actactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg 9721 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc 9781 accttcgggt gggcctttct gcgtttatat actagagttg acactgcccg gaagggcagg 9841 ttataattac tagagcttgc ataaagagga gaaatactag atgactataa tgataaaaaa 9901 atcggatttt ttggcaattc catcggagga gtataaaggt attctaagtc ttcgttatca 9961 agtgtttaag caaagacttg agtgggactt agttgtagaa aataaccttg aatcagatga 10021 gtatgataac tcaaatgcag aatatattta tgcttgtgat gatactgaaa atgtaagtgg 10081 atgctggcgt ttattaccta caacaggtga ttatatgctg aaaagtgttt ttcctgaatt 10141 gcttggtcaa cagagtgctc ccaaagatcc taatatagtc gaattaagtc gttttgctgt 10201 aggtaaaaat agctcaaaga taaataactc tgctagtgaa attacaatga aactatttga 10261 agctatatat aaacacgctg ttagtcaagg tattacagaa tatgtaacag taacatcaac 10321 agcaatagag cgatttttaa agcgtattaa agttccttgt catcgtattg gagacaaaga 10381 aattcatgta ttaggtgata ctaaatcggt tgtattgtct atgcctatta atgaacagtt 10441 taaaaaagca gtcttaaatt aataacgctg atagtgctag tgtagatcgc tactagagcc 10501 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt 10561 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc 10621 gtttatatac tagagacctg taggatcgta caggtttacg caagaaaatg gtttgttata 10681 gtcgaataaa tactagagag ctaaggaagc taaaatggag aaaaaaatca cgggatatac 10741 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc 10801 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa 10861 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaacgc 10921 tcacccggag tttcgtatgg ccatgaaaga cggtgagctg gtgatctggg atagtgttca 10981 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcgtccctct ggagtgaata 11041 ccacgacgat ttccggcagt ttctccacat atattcgcaa gatgtggcgt gttacggtga 11101 aaacctggcc tatttcccta aagggtttat tgagaatatg ttttttgtct cagccaatcc 11161 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc 11221 cgttttcacg atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat 11281 ccaggttcat catgccgttt gtgatggctt ccatgtcggc cgcatgctta atgaattaca 11341 acagtactgt gatgagtggc agggcggggc gtaataatac tagaggaggt actagatgac 11401 ggtctgcgca aaaaaacacg ttcatctcac tcgcgatgct gcggagcagt tactggctga 11461 tattgatcga cgccttgatc agttattgcc cgtggaggga gaacgggatg ttgtgggtgc 11521 cgcgatgcgt gaaggtgcgc tggcaccggg aaaacgtatt cgccccatgt tgctgttgct 11581 gaccgcccgc gatctgggtt gcgctgtcag ccatgacgga ttactggatt tggcctgtgc 11641 ggtggaaatg gtccacgcgg cttcgctgat ccttgacgat atgccctgca tggacgatgc 11701 gaagctgcgg cgcggacgcc ctaccattca ttctcattac ggagagcatg tggcaatact 11761 ggcggcggtt gccttgctga gtaaagcctt tggcgtaatt gccgatgcag atggcctcac 11821 gccgctggca aaaaatcggg cggtttctga actgtcaaac gccatcggca tgcaaggatt 11881 ggttcagggt cagttcaagg atctgtctga aggggataag ccgcgcagcg ctgaagctat 11941 tttgatgacg aatcacttta aaaccagcac gctgttttgt gcctccatgc agatggcctc 12001 gattgttgcg aatgcctcca gcgaagcgcg tgattgcctg catcgttttt cacttgatct 12061 tggtcaggca tttcaactgc tggacgattt gaccgatggc atgaccgaca ccggtaagga 12121 tagcaatcag gacgccggta aatcgacgct ggtcaatctg ttaggcccga gggcggttga 12181 agaacgtctg agacaacatc ttcagcttgc cagtgagcat ctctctgcgg cctgccaaca 12241 cgggcacgcc actcaacatt ttattcaggc ctggtttgac aaaaaactcg ctgccgtcag 12301 ttaataatac tagagctcaa ggaggtacta gatgaataat ccgtcgttac tcaatcatgc 12361 ggtcgaaacg atggcagttg gctcgaaaag ttttgcgaca gcctcaaagt tatttgatgc 12421 aaaaacccgg cgcagcgtac tgatgctcta cgcctggtgc cgccattgtg acgatgttat 12481 tgacgatcag acgctgggct ttcaggcccg gcagcctgcc ttacaaacgc ccgaacaacg 12541 tctgatgcaa cttgagatga aaacgcgcca ggcctatgca ggatcgcaga tgcacgaacc 12601 ggcgtttgcg gcttttcagg aagtggctat ggctcatgat atcgccccgg cttacgcgtt 12661 tgatcatctg gaaggcttcg ccatggatgt acgcgaagcg caatacagcc aactggatga 12721 tacgctgcgc tattgctatc acgttgcagg cgttgtcggc ttgatgatgg cgcaaatcat 12781 gggcgtgcgg gataacgcca cgctggaccg cgcctgtgac cttgggctgg catttcagtt 12841 gaccaatatt gctcgcgata ttgtggacga tgcgcatgcg ggccgctgtt atctgccggc 12901 aagctggctg gagcatgaag gtctgaacaa agagaattat gcggcacctg aaaaccgtca 12961 ggcgctgagc cgtatcgccc gtcgtttggt gcaggaagca gaaccttact atttgtctgc 13021 cacagccggc ctggcagggt tgcccctgcg ttccgcctgg gcaatcgcta cggcgaagca 13081 ggtttaccgg aaaataggtg tcaaagttga acaggccggt cagcaagcct gggatcagcg 13141 gcagtcaacg accacgcccg aaaaattaac gctgctgctg gccgcctctg gtcaggccct 13201 tacttcccgg atgcgggctc atcctccccg ccctgcgcat ctctggcagc gcccgctcta 13261 ataatactag agctcaagga ggtactagat gaaaccaact acggtaattg gtgcaggctt 13321 cggtggcctg gcactggcaa ttcgtctaca agctgcgggg atccccgtct tactgcttga 13381 acaacgtgat aaacccggcg gtcgggctta tgtctacgag gatcaggggt ttacctttga 13441 tgcaggcccg acggttatca ccgatcccag tgccattgaa gaactgtttg cactggcagg 13501 aaaacagtta aaagagtatg tcgaactgct gccggttacg ccgttttacc gcctgtgttg 13561 ggagtcaggg aaggtcttta attacgataa cgatcaaacc cggctcgaag cgcagattca 13621 gcagtttaat ccccgcgatg tcgaaggtta tcgtcagttt ctggactatt cacgcgcggt 13681 gtttaaagaa ggctatctaa agctcggtac tgtccctttt ttatcgttca gagacatgct 13741 tcgcgccgca cctcaactgg cgaaactgca agcatggaga agcgtttaca gtaaggttgc 13801 cagttacatc gaagatgaac atctgcgcca ggcgttttct ttccactcgc tgttggtggg 13861 cggcaatccc ttcgccacct catccattta tacgttgata cacgcgctgg agcgtgagtg 13921 gggcgtctgg tttccgcgtg gcggcaccgg cgcattagtt caggggatga taaagctgtt 13981 tcaggatctg ggtggcgaag tcgtgttaaa cgccagagtc agccatatgg aaacgacagg 14041 aaacaagatt gaagccgtgc atttagagga cggtcgcagg ttcctgacgc aagccgtcgc 14101 gtcaaatgca gatgtggttc atacctatcg cgacctgtta agccagcacc ctgccgcggt 14161 taagcagtcc aacaaactgc aaactaagcg catgagtaac tctctgtttg tgctctattt 14221 tggtttgaat caccatcatg atcagctcgc gcatcacacg gtttgtttcg gcccgcgtta 14281 ccgcgagctg attgacgaaa tttttaatca tgatggcctc gcagaggact tctcacttta 14341 tctgcacgcg ccctgtgtca cggattcgtc actggcgcct gaaggttgcg gcagttacta 14401 tgtgttggcg ccggtgccgc atttaggcac cgcgaacctc gactggacgg ttgaggggcc 14461 aaaactacgc gaccgtattt ttgcgtacct tgagcagcat tacatgcctg gcttacggag 14521 tcagctggtc acgcaccgga tgtttacgcc gtttgatttt cgcgaccagc ttaatgccta 14581 tcatggctca gccttttctg tggagcccgt tcttacccag agcgcctggt ttcggccgca 14641 taaccgcgat aaaaccatta ctaatctcta cctggtcggc gcaggcacgc atcccggcgc 14701 aggcattcct ggcgtcatcg gctcggcaaa agcgacagca ggtttgatgc tggaggatct 14761 gatataataa tactagagga ggtactagat gcaaccgcat tatgatctga ttctcgtggg 14821 ggctggactc gcgaatggcc ttatcgccct gcgtcttcag cagcagcaac ctgatatgcg 14881 tattttgctt atcgacgccg caccccaggc gggcgggaat catacgtggt catttcacca 14941 cgatgatttg actgagagcc aacatcgttg gatagctccg ctggtggttc atcactggcc 15001 cgactatcag gtacgctttc ccacacgccg tcgtaagctg aacagcggct acttttgtat 15061 tacttctcag cgtttcgctg aggttttaca gcgacagttt ggcccgcact tgtggatgga 15121 taccgcggtc gcagaggtta atgcggaatc tgttcggttg aaaaagggtc aggttatcgg 15181 tgcccgcgcg gtgattgacg ggcggggtta tgcggcaaat tcagcactga gcgtgggctt 15241 ccaggcgttt attggccagg aatggcgatt gagccacccg catggtttat cgtctcccat 15301 tatcatggat gccacggtcg atcagcaaaa tggttatcgc ttcgtgtaca gcctgccgct 15361 ctcgccgacc agattgttaa ttgaagacac gcactatatt gataatgcga cattagatcc 15421 tgaatgcgcg cggcaaaata tttgcgacta tgccgcgcaa cagggttggc agcttcagac 15481 actgctgcga gaagaacagg gcgccttacc cattactctg tcgggcaatg ccgacgcatt 15541 ctggcagcag cgccccctgg cctgtagtgg attacgtgcc ggtctgttcc atcctaccac 15601 cggctattca ctgccgctgg cggttgccgt ggccgaccgc ctgagtgcac ttgatgtctt 15661 tacgtcggcc tcaattcacc atgccattac gcattttgcc cgcgagcgct ggcagcagca 15721 gggctttttc cgcatgctga atcgcatgct gtttttagcc ggacccgccg attcacgctg 15781 gcgggttatg cagcgttttt atggtttacc tgaagattta attgcccgtt tttatgcggg 15841 aaaactcacg ctgaccgatc ggctacgtat tctgagcggc aagccgcctg ttccggtatt 15901 agcagcattg caagccatta tgacgactca tcgttaataa tactagagct ctaccggaga 15961 aattatgttg tggatttgga atgccctgat cgttttcgtt accgtgattg gcatggaagt 16021 gattgctgca ctggcacaca aatacatcat gcacggctgg ggttggggat ggcatctttc 16081 acatcatgaa ccgcgtaaag gtgcgtttga agttaacgat ctttatgccg tggtttttgc 16141 tgcattatcg atcctgctga tttatctggg cagtacagga atgtggccgc tccagtggat 16201 tggcgcaggt atgacggcgt atggattact ctattttatg gtgcacgacg ggctggtgca 16261 tcaacgttgg ccattccgct atattccacg caagggctac ctcaaacggt tgtatatggc 16321 gcaccgtatg catcacgccg tcaggggcaa agaaggttgt gtttcttttg gcttcctcta 16381 tgcgccgccc ctgtcaaaac ttcaggcgac gctccgggaa agacatggcg ctagagcggg 16441 cgctgccaga gatgcgcagg gcggggagga tgagcccgca tccgggaagt aataatacta 16501 gagctcaagg aggtactaga tgactgctgc tgttgctgag cctagaatcg ttcctagaca 16561 gacttggatc ggacttactc ttgctggaat gatcgttgct ggatggggat ctcttcacgt 16621 ttacggagtt tacttccaca gatggggaac ttcttctctt gttatcgttc ctgctatcgt 16681 tgctgttcag acttggcttt ctgttggact tttcatcgtt gctcatgatg ctatgcacgg 16741 atctcttgct cctggaagac ctagacttaa cgctgctgtt ggaagactta ctcttggact 16801 ttacgctgga ttcagattcg atagacttaa gactgctcac cacgctcacc acgctgctcc 16861 tggaactgct gatgatcctg atttctacgc tcctgctcct agagctttcc ttccttggtt 16921 ccttaacttc ttcagaactt acttcggatg gagagagatg gctgttctta ctgctcttgt 16981 tcttatcgct cttttcggac ttggagctag acctgctaac cttcttactt tctgggctgc 17041 tcctgctctt ctttctgctc ttcagctttt cactttcgga acttggcttc ctcacagaca 17101 cactgatcag cctttcgctg atgctcacca cgctagatct tctggatacg gacctgttct 17161 ttctcttctt acttgcttcc acttcggaag acaccacgag caccacctta ctccttggag 17221 accttggtgg agactttgga gaggagagtc ttgatactag agaactttaa gaaggagata 17281 tacatatgca ccatcatcat catcattctt ctggtctggt gccacgcggt tctggtatga 17341 aagaaaccgc tgctgctaaa ttcgaacgcc agcacatgga cagcccagat ctgggtaccg 17401 gtggtggctc cggtattgag ggtcgcatga tgtccttttc cgtactgcaa gtgaaacgtc 17461 tgcaaccgga actgattaca ccggcaaaat caacccctca ggaaaccaaa tttctgagcg 17521 acatcgatga tcaagaatct ctgcgcgttc agattccgat tatcatgtgt tataaagaca 17581 acccgagcct gaataaaaac cgtaacccgg tcaaagccat tcgtgaagca ctgtcacgtg 17641 ccctggtcta ttattatcca ctggccggtc gtctgcgtga aggtccaaac cgtaaactgg 17701 tggtcgattg taacggcgaa ggcatcctgt ttgtggaagc ttccgccgat gttacgctgg 17761 aacagctggg ggacaaaatc ctgcctcctt gtcctctgct ggaggagttt ctgtataact 17821 tcccgggttc tgatggcatt atcgactgcc cactgctgct gattcaagtg acctgtctga 17881 cttgtggagg gttcatcctg gctctgcgcc tgaaccatac aatgtgtgat gccgccggtc 17941 tgctgctgtt tctgaccgct attgccgaaa tggcccgtgg cgctcatgcc cctagtattc 18001 tgcctgtctg ggagcgtgaa ctgctgtttg ctcgtgatcc tcctcgtatc acctgtgccc 18061 accatgaata tgaggacgtg attggccact cagatgggtc ttatgccagc tcaaatcaga 18121 gtaatatggt ccaacgctcg ttctattttg gcgccaaaga gatgcgcgta ctgcgtaaac 18181 aaattccgcc acacctgatt agcacttgta gcaccttcga tctgatcacc gcttgtctgt 18241 ggaaatgtcg taccctggca ctgaacatta acccgaaaga ggccgttcgt gtttcgtgta 18301 ttgtgaatgc tcgtggtaaa cacaacaacg ttcgcctgcc tctgggttat tatggtaacg 18361 cctttgcctt tccggcagca atttcaaaag ccgaacctct gtgtaaaaac ccgctgggat 18421 atgctctgga actggtaaaa aaagcgaaag cgactatgaa cgaagagtat ctgcgttctg 18481 ttgctgatct gctggtactg cgcggtcgcc ctcagtatag tagtaccggc tcctatctga 18541 tcgttagcga caacacccgt gtaggcttcg gagatgtgaa ttttggctgg ggtcagcctg 18601 tttttgctgg accggttaaa gcactggatc tgatctcgtt ttatgtccaa cacaaaaaca 18661 acaccgagga tggcattctg gttccgatgt gtctgcctag tagcgctatg gaacgctttc 18721 agcaggaact ggagcgtatt acccaggaac caaaagagga catctgtaac aatctgcgct 18781 caacgtccca gtaaggctct aactctcctc tggccatggc gatatcggat ccaaattcga 18841 gctccgtcga caagcttgcg gccgcactcg agcaccacca ccaccaccac tgagatccgg 18901 ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgat actagagcta 18961 gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttg //