LOCUS       BBa_K396000             1654 bp    DNA     linear       03-May-2025
DEFINITION  T7/CI Hybrid Promoter with GFP (composite with LuxR generator)
ACCESSION   BBa_K396000
VERSION     BBa_K396000.1  
FEATURES             Location/Qualifiers
     CDS             4..757
                     /label=LuxR
     promoter        784..818
                     /label=Constitutive promoter
     promoter        833..855
                     /label=T7 promoter 
     misc_feature    855..868
                     /label=Lambda CI (OR1 operator)
     CDS             901..1616
                     /label=GFP
     misc_feature    1616..1654
                     /label=LVA
ORIGIN
        1 ttattaattt ttaaagtatg ggcaatcaat tgctcctgtt aaaattgctt tagaaatact
       61 ttggcagcgg tttgttgtat tgagtttcat ttgcgcattg gttaaatgga aagtgacagt
      121 acgctcactg caacctaata tttttgaaat atcccaagag ctttttcctt cgcatgccca
      181 cgctaaacat tctttttctc ttttggttaa atcgttgttt gatttattat ttgctatatt
      241 tatttttcga taattatcaa ctagagaagg aacaattaat ggtatgttca tacacgcatg
      301 taaaaataaa ctatctatat agttgtcttt ttctgaatgt gcaaaactaa gcattccgaa
      361 gccattgtta gccgtatgaa tagggaaact aaacccagtg ataagacctg atgttttcgc
      421 ttctttaatt acatttggag attttttatt tacagcattg ttttcaaata tattccaatt
      481 aattggtgaa tgattggagt tagaataatc tactatagga tcatatttta ttaaattagc
      541 gtcatcataa tattgcctcc attttttagg gtaattatct aggattgaaa tatcagattt
      601 aaccatagaa tgaggataaa tgatcgcgag taaataatat tcacaatgta ccattttagt
      661 catatcagat aagcattgat taatatcatt attgcttcta caagctttaa ttttattaat
      721 tattctgtat gtgtcgtcgg catttatgtt tttcatctag tatttctcct ctttctctag
      781 tagctagcac tgtacctagg actgagctag ccgtcaattt tctcgagttt ttaatacgac
      841 tcactatagg gagatctggc ggtgataatg gttgcaaaga ggagaaatac tagccatggg
      901 gatgagtaaa ggagaagaac ttttcactgg agttgtccca attcttgttg aattagatgg
      961 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
     1021 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccgt ggccaacact
     1081 tgtcactact ttctcttatg gtgttcaatg cttttcccgt tatccggatc atatgaaacg
     1141 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaacgca ctatatcttt
     1201 caaagatgac gggaactaca agacgcgtgc tgaagtcaag tttgaaggtg atacccttgt
     1261 taatcgtatc gagttaaaag gtattgattt taaagaagat ggaaacattc tcggacacaa
     1321 actcgagtac aactataact cacacaatgt atacatcacg gcagacaaac aaaagaatgg
     1381 aatcaaagct aacttcaaaa ttcgccacaa cattgaagat ggatccgttc aactagcaga
     1441 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
     1501 cctgtcgaca caatctgccc tttcgaaaga tcccaacgaa aagcgtgacc acatggtcct
     1561 tcttgagttt gtaactgctg ctgggattac acatggcatg gatgagctct acaaagctgc
     1621 aaacgacgaa aactacgctt tagtagctta ataa
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