LOCUS       BBa_K396006             1595 bp    DNA     linear       22-Nov-2024
DEFINITION  GFP pulse generator with pLux/cI434 hybrid promoter
ACCESSION   BBa_K396006
VERSION     BBa_K396006.1  
FEATURES             Location/Qualifiers
     misc_feature    1..839
                     /label=BBa_K396001
     CDS             1..756
                     /label=GFPuv 
     misc_feature    1..61
                     /label=BBa_K396002
     misc_feature    4..36
                     /label=LVA
     misc_feature    779..793
                     /label=cI434 OR1
     promoter        793..798
                     /label=-10
     misc_feature    800..814
                     /label=cI434 OR2
     promoter        815..820
                     /label=-35
     misc_feature    819..839
                     /label=Lux Box
     misc_feature    854..909
                     /label=BBa_R1062
     misc_feature    854..873
                     /label=LuxR/HSL
     promoter        874..879
                     /label=-35
     promoter        895..901
                     /label=-10
     misc_feature    910..923
                     /label=BBa_B0031
     CDS             930..1595
                     /label=cI 434
     misc_feature    930..1595
                     /label=BBa_C0052
     misc_feature    1560..1592
                     /label=LVA
     RBS             763..769
                     /label=RBS(weak)
ORIGIN
        1 ttaagctact aaagcgtagt tttcgtcgtt tgcagctttg tagagctcat ccatgccatg
       61 tgtaatccca gcagcagtta caaactcaag aaggaccatg tggtcacgct tttcgttggg
      121 atctttcgaa agggcagatt gtgtcgacag gtaatggttg tctggtaaaa ggacggggcc
      181 atcgccaatt ggagtatttt gttgataatg gtctgctagt tgaacggatc catcttcaat
      241 gttgtggcga attttgaagt tagctttgat tccattcttt tgtttgtctg ccgtgatgta
      301 tacattgtgt gagttatagt tgtactcgag tttgtgtccg agaatgtttc catcttcttt
      361 aaaatcaata ccttttaact cgatacgatt aacaagggta tcaccttcaa acttgacttc
      421 agcacgcgtc tggtagttcc cgtcatcttt gaaagatata gtgcgttcct gtacataacc
      481 ttcgggcatg gcactcttga aaaagtcatg ccgtttcata tgatccggat aacgggaaaa
      541 gcattgaaca ccataagaga aagtagtgac aagtgttggc cacggaacag gtagttttcc
      601 agtagtgcaa ataaatttaa gggtaagttt tccgtatgtt gcatcacctt caccctctcc
      661 actgacagaa aatttgtgcc cattaacatc accatctaat tcaacaagaa ttgggacaac
      721 tccagtgaaa agttcttctc ctttactcat ccccatctag tactttcctg tgaagcttca
      781 agaaagtttg taactataac aagatacatt gtaacgtaaa cctgtacgat cctacaggtg
      841 cattagctta tggtacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag
      901 tcgaataaat cacacaggaa acctactaga tgagtatttc ttccagggta aaaagcaaaa
      961 gaatccagct tggacttaac caggctgaac ttgctcaaaa ggtggggact acccagcagt
     1021 ctatagagca gctcgaaaac ggtaaaacta agcgaccacg ctttttacca gaacttgcgt
     1081 cagctcttgg cgtaagtgtt gactggctgc tcaatggcac ctctgattcg aatgttagat
     1141 ttgttgggca cgttgagccc aaagggaaat atccattgat tagcatggtt agagctggtt
     1201 cgtggtgtga agcttgtgaa ccctacgata tcaaggacat tgatgaatgg tatgacagtg
     1261 acgttaactt attaggcaat ggattctggc tgaaggttga aggtgattcc atgacctcac
     1321 ctgtaggtca aagcatccct gaaggtcata tggtgttagt agatactgga cgggagccag
     1381 tgaatggaag ccttgttgta gccaaactga ctgacgcgaa cgaagcaaca ttcaagaaac
     1441 tggtcataga tggcggtcag aagtacctga aaggcctgaa tccttcatgg cctatgactc
     1501 ctatcaacgg aaactgcaag attatcggtg ttgtcgtgga agcgagggta aaattcgtag
     1561 ctgcaaacga cgaaaactac gctttagtag cttaa
//