BBa_K396006 1 BBa_K396006 GFP pulse generator with pLux/cI434 hybrid promoter 2010-10-20T11:00:00Z 2015-05-08T01:12:22Z Ron Weiss. Spatiotemporal control of gene expression with pulse-generating networks When there is AHL induction, GFP generats as a pulse because of cI434 repression. false false _519_ 0 6729 9 It's complicated false hybrid promoter has lux box in its upstream and cI434 operation site(OR1,OR2)in its downstream. The RBS of cI434 is weak and GFP is designed destabilized with LVA tag. false Ling Li annotation2108819 1 -10 range2108819 1 895 901 annotation2108811 1 cI434 OR1 range2108811 1 779 793 annotation2108809 1 BBa_K396002 range2108809 1 1 61 annotation2108810 1 LVA range2108810 1 4 36 annotation2108822 1 BBa_C0052 range2108822 1 930 1595 annotation2108813 1 cI434 OR2 range2108813 1 800 814 annotation2108814 1 -35 range2108814 1 815 820 annotation2108820 1 BBa_B0031 range2108820 1 910 923 annotation2108824 1 RBS(weak) range2108824 1 763 769 annotation2108816 1 BBa_R1062 range2108816 1 854 909 annotation2108812 1 -10 range2108812 1 793 798 annotation2108823 1 LVA range2108823 1 1560 1592 annotation2108807 1 BBa_K396001 range2108807 1 1 839 annotation2108817 1 LuxR/HSL range2108817 1 854 873 annotation2108821 1 cI 434 range2108821 1 930 1595 annotation2108808 1 GFPuv range2108808 1 1 756 annotation2108818 1 -35 range2108818 1 874 879 annotation2108815 1 Lux Box range2108815 1 819 839 BBa_K396006_sequence 1 ttaagctactaaagcgtagttttcgtcgtttgcagctttgtagagctcatccatgccatgtgtaatcccagcagcagttacaaactcaagaaggaccatgtggtcacgcttttcgttgggatctttcgaaagggcagattgtgtcgacaggtaatggttgtctggtaaaaggacggggccatcgccaattggagtattttgttgataatggtctgctagttgaacggatccatcttcaatgttgtggcgaattttgaagttagctttgattccattcttttgtttgtctgccgtgatgtatacattgtgtgagttatagttgtactcgagtttgtgtccgagaatgtttccatcttctttaaaatcaataccttttaactcgatacgattaacaagggtatcaccttcaaacttgacttcagcacgcgtctggtagttcccgtcatctttgaaagatatagtgcgttcctgtacataaccttcgggcatggcactcttgaaaaagtcatgccgtttcatatgatccggataacgggaaaagcattgaacaccataagagaaagtagtgacaagtgttggccacggaacaggtagttttccagtagtgcaaataaatttaagggtaagttttccgtatgttgcatcaccttcaccctctccactgacagaaaatttgtgcccattaacatcaccatctaattcaacaagaattgggacaactccagtgaaaagttcttctcctttactcatccccatctagtactttcctgtgaagcttcaagaaagtttgtaactataacaagatacattgtaacgtaaacctgtacgatcctacaggtgcattagcttatggtacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatcacacaggaaacctactagatgagtatttcttccagggtaaaaagcaaaagaatccagcttggacttaaccaggctgaacttgctcaaaaggtggggactacccagcagtctatagagcagctcgaaaacggtaaaactaagcgaccacgctttttaccagaacttgcgtcagctcttggcgtaagtgttgactggctgctcaatggcacctctgattcgaatgttagatttgttgggcacgttgagcccaaagggaaatatccattgattagcatggttagagctggttcgtggtgtgaagcttgtgaaccctacgatatcaaggacattgatgaatggtatgacagtgacgttaacttattaggcaatggattctggctgaaggttgaaggtgattccatgacctcacctgtaggtcaaagcatccctgaaggtcatatggtgttagtagatactggacgggagccagtgaatggaagccttgttgtagccaaactgactgacgcgaacgaagcaacattcaagaaactggtcatagatggcggtcagaagtacctgaaaggcctgaatccttcatggcctatgactcctatcaacggaaactgcaagattatcggtgttgtcgtggaagcgagggtaaaattcgtagctgcaaacgacgaaaactacgctttagtagcttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z