LOCUS BBa_K396007 3542 bp DNA linear 23-Dec-2024 DEFINITION T7 RNA Polymerase pulse generator ACCESSION BBa_K396007 VERSION BBa_K396007.1 FEATURES Location/Qualifiers misc_feature 4..33 /label=LVA CDS 1..2703 /label=T7 RNA Polymerase misc_feature 2683..2700 /label=His tag RBS 2711..2716 /label=RBS weak misc_feature 2762..2786 /label=Lux Box promoter 2762..2767 /label=-35 misc_feature 2747..2761 /label=cI434 OR2 promoter 2740..2745 /label=-10 misc_feature 2726..2740 /label=cI434 0R 1 promoter 2820..2856 /label=R1062 misc_feature 2802..2822 /label=Lux Box RBS 2863..2868 /label=RBS weak CDS 2877..3542 /label=C0052 misc_feature 3507..3539 /label=LVA ORIGIN 1 ttaagctact aaagcgtagt tttcgtcgtt tgcagccgcg aacgcgaagt ccgactctaa 61 gatgtcacgg aggttcaagt tacctttagc cggaagtgct ggcattttgt ccaattgaga 121 ctcgtgcaac tggtcagcga actggtcgta gaaatcagcc agtacatcac aagactcata 181 tgtgtcaacc atagtttcgc gcactgcttt gaacaggttc gcagcgtcag ccggaatggt 241 accgaaggag tcgtgaatca gtgcaaaaga ttcgattccg tacttctcgt gtgcccacac 301 tacagtctta cgaaggtggc taccgtcttg gctgtgtaca aagttaggag cgataccaga 361 ctcctgtttg tgtgcatcaa tctcgctatc tttgttggtg ttaatggtag gctgtaagcg 421 gaactgaccg aggaacatca ggttcaagcg cgtctgaata ggcttcttgt attcctgcca 481 cacagggaaa ccatcaggag ttacccaatg cacagcgcaa cgcttgcgaa gaatctctcc 541 agtcttctta tctttgacct cagcagccag cagcttagca gcagacttaa gccagttcat 601 tgcttcaacc gcagctacca ccgtcacgct cacagattcc caaatcagct tagccatgta 661 tccagcagcc tgattcggct gagtgaacat cagacccttg ccggaatcaa tagctggctg 721 aatggtatct tccagcactt gttgacggaa gccgaactct ttggacccgt aagccagcgt 781 catgactgaa cgcttagtca cactgcgagt aacaccgtaa gccagccatt gaccagccag 841 tgccttagtg cccagcttga ctttctcaga gatttcacca gtgttctcat cggtcacggt 901 aactacttcg ttatcggtcc cattgattgc gtctgcttgt agaatctcgt tgactttctt 961 agcaacaatc ccgtagatgt cctgaacggt ttcactagga agcaagttaa ccgcgcgacc 1021 acctacctca tctcggagca tcgcggagaa gtgctggatg ccagagcaag acccgtcaaa 1081 cgccagcgga agggagcagt tatagctcag gccgtggtgc tgtaccccag cgtactcaaa 1141 gcagaacgca aggaagcaga acggagaatc ttgctcagcc caccaagtgt tctccagtgg 1201 agacttagcg caagccatga tgttctcgtg gttttcctca atgaacttga tgcgctcagg 1261 gaacggaacc ttatcgacac ccgcacagtt tgcaccgtgg attttcagcc agtagtaacc 1321 ttccttaccg attggtttac ctttcgccag cgtaagcagt cctttggtca tatcgttacc 1381 ttgcgggttg aacattgaca cagcgtaaac acgaccgcgc cagtccatgt tgtaagggaa 1441 ccagatggcc ttatggttag caaacttatt ggcttgctca agcatgaact caaggctgat 1501 acggcgagac ttgcgagcct tgtccttgcg gtacacagca gcggcagcac gtttccacgc 1561 ggtgagagcc tcaggattca tgtcgatgtc ttccggtttc atcgggagtt cttcacgctc 1621 aatcgcaggg atgtcctcga ccggacaatg cttccacttg gtgattacgt tggcgaccgc 1681 taggactttc ttgttgattt tccatgcggt gttttgcgca atgttaatcg ctttgtacac 1741 ctcaggcatg taaacgtctt cgtagcgcat cagtgctttc ttactgtgag tacgcaccag 1801 cgccagagga cgacgaccgt tagcccaata gccaccacca gtaatgccag tccacggctt 1861 aggaggaact acgcaaggtt ggaacatcgg agagatgcca gccagcgcac ctgcacgggt 1921 tgcgatagcc tcagcgtatt caggtgcgag ttcgatagtc tcagagtctt gacctactac 1981 gccagcattt tggcggtgta agctaaccat tccggttgac tcaatgagca tctcgatgca 2041 gcgtactcct acatgaatag agtcttcctt atgccacgaa gaccacgcct cgccaccgag 2101 tagaccctta gagagcatgt cagcctcgac aacttgcata aatgctttct tgtagacgtg 2161 ccctacgcgc ttgttgagtt gttcctcaac gtttttcttg aagtgcttag cttcaaggtc 2221 acggatacga ccgaagcgag cctcgtcctc aatggcccga ccgattgcgc ttgctacagc 2281 ctgaacggtt gtattgtcag cactggttag gcaagccaga gtggtcttaa tggtgatgta 2341 cgctacggct tccggcttga tttcttgcag gaactggaag gctgtcgggc gcttgccgcg 2401 cttagctttc acttcctcaa accagtcgtt gatgcgtgca atcatcttag ggagtagggt 2461 agtgatgaga ggcttggcgg cagcgttatc cgcaacctca ccagctttaa gttgacgctc 2521 aaacatcttg cggaagcgtg cttcacccat ctcgtaagac tcatgctcaa gggccaactg 2581 ttcgcgagct aaacgctcac cgtaatggtc agccagagtg ttgaacggga tagcagccag 2641 ttcgatgtca gagaagtcgt tcttagcgat gttaatcgtg ttgtggtgat ggtgatgatg 2701 catctagtac tttcctgtga agcttcaaga aagtttgtaa ctataacaag atacattgta 2761 acgtaaacct gtacgatcct acaggtgcat tagcttatgg tacctgtagg atcgtacagg 2821 tttacgcaag aaaatggttt gttatagtcg aataaatcac acaggaaacc tactagatga 2881 gtatttcttc cagggtaaaa agcaaaagaa tccagcttgg acttaaccag gctgaacttg 2941 ctcaaaaggt ggggactacc cagcagtcta tagagcagct cgaaaacggt aaaactaagc 3001 gaccacgctt tttaccagaa cttgcgtcag ctcttggcgt aagtgttgac tggctgctca 3061 atggcacctc tgattcgaat gttagatttg ttgggcacgt tgagcccaaa gggaaatatc 3121 cattgattag catggttaga gctggttcgt ggtgtgaagc ttgtgaaccc tacgatatca 3181 aggacattga tgaatggtat gacagtgacg ttaacttatt aggcaatgga ttctggctga 3241 aggttgaagg tgattccatg acctcacctg taggtcaaag catccctgaa ggtcatatgg 3301 tgttagtaga tactggacgg gagccagtga atggaagcct tgttgtagcc aaactgactg 3361 acgcgaacga agcaacattc aagaaactgg tcatagatgg cggtcagaag tacctgaaag 3421 gcctgaatcc ttcatggcct atgactccta tcaacggaaa ctgcaagatt atcggtgttg 3481 tcgtggaagc gagggtaaaa ttcgtagctg caaacgacga aaactacgct ttagtagctt 3541 aa //