LOCUS       BBa_K396012              914 bp    DNA     linear       15-Jun-2024
DEFINITION  GFP reporter repressed by LuxR and HSL
ACCESSION   BBa_K396012
VERSION     BBa_K396012.1  
FEATURES             Location/Qualifiers
     promoter        1..6
                     /label=-35
     promoter        25..30
                     /label=-10
     misc_feature    6..25
                     /label=Lux Box
     promoter        1..30
                     /label=BBa_R0061
     RBS             39..51
                     /label=RBS-3\Weak
     RBS             45..48
                     /label=RBS
     misc_feature    39..51
                     /label=BBa_B0032
     RBS             39..51
                     /label=BBa_B0032
     CDS             58..777
                     /label=GFP protein
     CDS             58..777
                     /label=BBa_E0040
     stem_loop       797..840
                     /label=stem_loop
     misc_feature    786..865
                     /label=BBa_B0010
     terminator      786..865
                     /label=BBa_B0010
     stem_loop       881..900
                     /label=T7 TE
     misc_feature    907..907
                     /label=stop
     polyA_site      901..914
                     /label=polya
     misc_feature    874..914
                     /label=BBa_B0012
     terminator      874..914
                     /label=BBa_B0012
     misc_feature    39..914
                     /label=BBa_E0240
ORIGIN
        1 ttgacacctg taggatcgta caggtataat tactagagtc acacaggaaa gtactagatg
       61 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      121 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      181 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
      241 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat
      301 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
      361 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
      421 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
      481 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
      541 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
      601 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
      661 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
      721 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac
      781 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      841 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      901 tttctgcgtt tata
//