BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K398030
1
BBa_K398030
ALDH generator (over-expression)
2010-10-06T11:00:00Z
2015-05-08T01:12:22Z
Genebank
ALDH, an aldehyde deyhydrogenase that facilitates the third step in alkane degradation, from n-alkanals to n-alkanoic acids, which can then be further degraded through β-oxidation
false
false
_506_
0
6322
9
It's complicated
false
Nucleotide sequence optimized for expression in E.coli
false
Kira Schipper
component2083587
1
BBa_R0040
component2083593
1
BBa_B0034
component2083597
1
BBa_K398006
annotation2083593
1
BBa_B0034
range2083593
1
63
74
annotation2083587
1
BBa_R0040
range2083587
1
1
54
annotation2083597
1
BBa_K398006
range2083597
1
81
1574
BBa_K398006
1
Bt-ALDH
Medium-Chain Aldehyde Dehydrogenase
2010-06-12T11:00:00Z
2015-05-08T01:12:22Z
Genbank
Aldehyde dehydrogenase from Geobacillus thermoleovorans B23.
false
false
_506_
0
6320
9
It's complicated
false
Nucleotide sequence optimized for expression in E.coli.
false
Mathias Voges
annotation2070757
1
Bt-ALDH
range2070757
1
1
1494
annotation2070756
1
stop
range2070756
1
1492
1494
annotation2070755
1
start
range2070755
1
1
3
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986787
1
-10
range1986787
1
43
48
annotation1986785
1
-35
range1986785
1
20
25
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986783
1
TetR 1
range1986783
1
1
19
BBa_B0034_sequence
1
aaagaggagaaa
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_K398006_sequence
1
atgaccatgatctctgctcagaccaccgaagctgacctgtggatcgacggtgaatggcgtccggctgcttctggtgaacgtttcgacgttatcgacccggctaccggcgaagttaccgctcgtgttgctaacgctggtgaagacgacgttgacgctgctgttgctatcgctgaagaagctttctctgttcgtcgttggctggctatctctccgctggaacgtggtcgtatcctgcgtcgtatcgctgaactgatccgtcagcaccactgcgaactggctcagctgatgacccgtgaaaacggtatgccgatcaacctggctctgttcatcgaaatcccgctggctgactgcttcgacttcttcgcttctctggttgttaaaccgcagggtgaagttctgccgttctctgttgctggttctgctccggactacatggcttggaccatgaaagaaccgatcggtgttgctggtctgatcaccccgtggaacttcccgctgctgatgccgacctggaaaatcgctccggctctggctgctggttgcaccatggttgttaaaccggctccggaaaccccgctgaccgctctgaaactggctgaaatctgccacgaagctggtgttccggaaggtgttatcaacgttctgccgggtctggacgaagctggtaaagctctggttcgtcacccgcgtgttccgaaaatcgctttcaccggcgaaaccgaaaccggccgtcacatcctgcaagctgctgctccgcacatcaaacgtgttaccctggaactgggtggtaaatctccgaacatcatcttcgctgacgctgacctggaacaggctgctaaatctgctctgttcggtgttttctacaactctggtcaggtttgccaggctggttctcgtatcctggttgaacgtaccgtttacgaaccgttcgttgaacgtctggctgaacgtgctaaaaaactgaaagttggtccgggtaccaacccgcgttctgacctgggtccggttatctctcgtgaacagtacgaaaaagttctgcgttacatcgaaatcggtaaacaggaaggtgctcgtctggctgctggtggtcgtgctctggacggtggtggtggtggttacttcatcgaaccgaccgttttcgctgacgtttctccgtctatgcgtatcgcttgcgaagaaatcttcggtccggttgctgctgttatcccgttcgctgacgaagaagaagctgttcgtatcgctaacggtaccatgtacggtctggctgctgctgtttggaccaacgacatcaaacgtgctctgcgtctggctcgtcgtgttaaatctggtaccgtttggctgaacacctaccaggttctgtctccgaccgttccgttcggtggttacaaacagtctggtctgggtcgtgaactgggtatgcaggctctggacgcttacctggaaaccaaaaccgttatctgcgacctgaacgaccgtccgatgaccctgttctaa
BBa_K398030_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaccatgatctctgctcagaccaccgaagctgacctgtggatcgacggtgaatggcgtccggctgcttctggtgaacgtttcgacgttatcgacccggctaccggcgaagttaccgctcgtgttgctaacgctggtgaagacgacgttgacgctgctgttgctatcgctgaagaagctttctctgttcgtcgttggctggctatctctccgctggaacgtggtcgtatcctgcgtcgtatcgctgaactgatccgtcagcaccactgcgaactggctcagctgatgacccgtgaaaacggtatgccgatcaacctggctctgttcatcgaaatcccgctggctgactgcttcgacttcttcgcttctctggttgttaaaccgcagggtgaagttctgccgttctctgttgctggttctgctccggactacatggcttggaccatgaaagaaccgatcggtgttgctggtctgatcaccccgtggaacttcccgctgctgatgccgacctggaaaatcgctccggctctggctgctggttgcaccatggttgttaaaccggctccggaaaccccgctgaccgctctgaaactggctgaaatctgccacgaagctggtgttccggaaggtgttatcaacgttctgccgggtctggacgaagctggtaaagctctggttcgtcacccgcgtgttccgaaaatcgctttcaccggcgaaaccgaaaccggccgtcacatcctgcaagctgctgctccgcacatcaaacgtgttaccctggaactgggtggtaaatctccgaacatcatcttcgctgacgctgacctggaacaggctgctaaatctgctctgttcggtgttttctacaactctggtcaggtttgccaggctggttctcgtatcctggttgaacgtaccgtttacgaaccgttcgttgaacgtctggctgaacgtgctaaaaaactgaaagttggtccgggtaccaacccgcgttctgacctgggtccggttatctctcgtgaacagtacgaaaaagttctgcgttacatcgaaatcggtaaacaggaaggtgctcgtctggctgctggtggtcgtgctctggacggtggtggtggtggttacttcatcgaaccgaccgttttcgctgacgtttctccgtctatgcgtatcgcttgcgaagaaatcttcggtccggttgctgctgttatcccgttcgctgacgaagaagaagctgttcgtatcgctaacggtaccatgtacggtctggctgctgctgtttggaccaacgacatcaaacgtgctctgcgtctggctcgtcgtgttaaatctggtaccgtttggctgaacacctaccaggttctgtctccgaccgttccgttcggtggttacaaacagtctggtctgggtcgtgaactgggtatgcaggctctggacgcttacctggaaaccaaaaccgttatctgcgacctgaacgaccgtccgatgaccctgttctaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z