BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_K398006 1 Bt-ALDH Medium-Chain Aldehyde Dehydrogenase 2010-06-12T11:00:00Z 2015-05-08T01:12:22Z Genbank Aldehyde dehydrogenase from Geobacillus thermoleovorans B23. false false _506_ 0 6320 9 It's complicated false Nucleotide sequence optimized for expression in E.coli. false Mathias Voges annotation2070755 1 start range2070755 1 1 3 annotation2070757 1 Bt-ALDH range2070757 1 1 1494 annotation2070756 1 stop range2070756 1 1492 1494 BBa_K398030 1 BBa_K398030 ALDH generator (over-expression) 2010-10-06T11:00:00Z 2015-05-08T01:12:22Z Genebank ALDH, an aldehyde deyhydrogenase that facilitates the third step in alkane degradation, from n-alkanals to n-alkanoic acids, which can then be further degraded through &#946;-oxidation false false _506_ 0 6322 9 It's complicated false Nucleotide sequence optimized for expression in E.coli false Kira Schipper component2083593 1 BBa_B0034 component2083597 1 BBa_K398006 component2083587 1 BBa_R0040 annotation2083593 1 BBa_B0034 range2083593 1 63 74 annotation2083597 1 BBa_K398006 range2083597 1 81 1574 annotation2083587 1 BBa_R0040 range2083587 1 1 54 BBa_R0040 1 p(tetR) TetR repressible promoter 2003-01-31T12:00:00Z 2015-05-08T01:14:14Z Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210. Released HQ 2013 Sequence for pTet inverting regulator driven by the TetR protein.</P> false true _1_ 0 24 7 In stock false <P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P> true June Rhee, Connie Tao, Ty Thomson, Louis Waldman annotation1986786 1 TetR 2 range1986786 1 26 44 annotation1986783 1 TetR 1 range1986783 1 1 19 annotation1986784 1 BBa_R0040 range1986784 1 1 54 annotation1986787 1 -10 range1986787 1 43 48 annotation1986785 1 -35 range1986785 1 20 25 BBa_B0034_sequence 1 aaagaggagaaa BBa_R0040_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac BBa_K398006_sequence 1 atgaccatgatctctgctcagaccaccgaagctgacctgtggatcgacggtgaatggcgtccggctgcttctggtgaacgtttcgacgttatcgacccggctaccggcgaagttaccgctcgtgttgctaacgctggtgaagacgacgttgacgctgctgttgctatcgctgaagaagctttctctgttcgtcgttggctggctatctctccgctggaacgtggtcgtatcctgcgtcgtatcgctgaactgatccgtcagcaccactgcgaactggctcagctgatgacccgtgaaaacggtatgccgatcaacctggctctgttcatcgaaatcccgctggctgactgcttcgacttcttcgcttctctggttgttaaaccgcagggtgaagttctgccgttctctgttgctggttctgctccggactacatggcttggaccatgaaagaaccgatcggtgttgctggtctgatcaccccgtggaacttcccgctgctgatgccgacctggaaaatcgctccggctctggctgctggttgcaccatggttgttaaaccggctccggaaaccccgctgaccgctctgaaactggctgaaatctgccacgaagctggtgttccggaaggtgttatcaacgttctgccgggtctggacgaagctggtaaagctctggttcgtcacccgcgtgttccgaaaatcgctttcaccggcgaaaccgaaaccggccgtcacatcctgcaagctgctgctccgcacatcaaacgtgttaccctggaactgggtggtaaatctccgaacatcatcttcgctgacgctgacctggaacaggctgctaaatctgctctgttcggtgttttctacaactctggtcaggtttgccaggctggttctcgtatcctggttgaacgtaccgtttacgaaccgttcgttgaacgtctggctgaacgtgctaaaaaactgaaagttggtccgggtaccaacccgcgttctgacctgggtccggttatctctcgtgaacagtacgaaaaagttctgcgttacatcgaaatcggtaaacaggaaggtgctcgtctggctgctggtggtcgtgctctggacggtggtggtggtggttacttcatcgaaccgaccgttttcgctgacgtttctccgtctatgcgtatcgcttgcgaagaaatcttcggtccggttgctgctgttatcccgttcgctgacgaagaagaagctgttcgtatcgctaacggtaccatgtacggtctggctgctgctgtttggaccaacgacatcaaacgtgctctgcgtctggctcgtcgtgttaaatctggtaccgtttggctgaacacctaccaggttctgtctccgaccgttccgttcggtggttacaaacagtctggtctgggtcgtgaactgggtatgcaggctctggacgcttacctggaaaccaaaaccgttatctgcgacctgaacgaccgtccgatgaccctgttctaa BBa_K398030_sequence 1 tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaccatgatctctgctcagaccaccgaagctgacctgtggatcgacggtgaatggcgtccggctgcttctggtgaacgtttcgacgttatcgacccggctaccggcgaagttaccgctcgtgttgctaacgctggtgaagacgacgttgacgctgctgttgctatcgctgaagaagctttctctgttcgtcgttggctggctatctctccgctggaacgtggtcgtatcctgcgtcgtatcgctgaactgatccgtcagcaccactgcgaactggctcagctgatgacccgtgaaaacggtatgccgatcaacctggctctgttcatcgaaatcccgctggctgactgcttcgacttcttcgcttctctggttgttaaaccgcagggtgaagttctgccgttctctgttgctggttctgctccggactacatggcttggaccatgaaagaaccgatcggtgttgctggtctgatcaccccgtggaacttcccgctgctgatgccgacctggaaaatcgctccggctctggctgctggttgcaccatggttgttaaaccggctccggaaaccccgctgaccgctctgaaactggctgaaatctgccacgaagctggtgttccggaaggtgttatcaacgttctgccgggtctggacgaagctggtaaagctctggttcgtcacccgcgtgttccgaaaatcgctttcaccggcgaaaccgaaaccggccgtcacatcctgcaagctgctgctccgcacatcaaacgtgttaccctggaactgggtggtaaatctccgaacatcatcttcgctgacgctgacctggaacaggctgctaaatctgctctgttcggtgttttctacaactctggtcaggtttgccaggctggttctcgtatcctggttgaacgtaccgtttacgaaccgttcgttgaacgtctggctgaacgtgctaaaaaactgaaagttggtccgggtaccaacccgcgttctgacctgggtccggttatctctcgtgaacagtacgaaaaagttctgcgttacatcgaaatcggtaaacaggaaggtgctcgtctggctgctggtggtcgtgctctggacggtggtggtggtggttacttcatcgaaccgaccgttttcgctgacgtttctccgtctatgcgtatcgcttgcgaagaaatcttcggtccggttgctgctgttatcccgttcgctgacgaagaagaagctgttcgtatcgctaacggtaccatgtacggtctggctgctgctgtttggaccaacgacatcaaacgtgctctgcgtctggctcgtcgtgttaaatctggtaccgtttggctgaacacctaccaggttctgtctccgaccgttccgttcggtggttacaaacagtctggtctgggtcgtgaactgggtatgcaggctctggacgcttacctggaaaccaaaaccgttatctgcgacctgaacgaccgtccgatgaccctgttctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z