BBa_S04459
1
BBa_S04459
R0011:B0032
2010-07-12T11:00:00Z
2015-05-08T01:14:40Z
false
true
_9_
0
6350
9
Not in stock
false
false
Pieter van Boheemen
component2073265
1
BBa_R0011
component2073271
1
BBa_B0032
annotation2073271
1
BBa_B0032
range2073271
1
64
76
annotation2073265
1
BBa_R0011
range2073265
1
1
54
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation7027
1
BBa_B0032
range7027
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation1709
1
RBS-3\Weak
range1709
1
1
13
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation2002
1
-10
range2002
1
43
48
annotation2001
1
lac O1
range2001
1
26
42
annotation2000
1
-35
range2000
1
20
25
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation1999
1
lac O1
range1999
1
3
19
BBa_K398202
1
BBa_K398202
Promotor R0011 + RBS B0032
2010-07-12T11:00:00Z
2015-05-08T01:12:22Z
iGEM 2010 Distribution plates
Construction intermediate of promotor R0011 and RBS B0032
true
false
_506_
0
6350
9
Discontinued
false
None
false
Pieter van Boheemen
component2073279
1
BBa_B0032
component2073273
1
BBa_R0011
annotation2073273
1
BBa_R0011
range2073273
1
1
54
annotation2073279
1
BBa_B0032
range2073279
1
64
76
BBa_K398202_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaag
BBa_B0032_sequence
1
tcacacaggaaag
BBa_S04459_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaag
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z