BBa_K398203
1
BBa_K398203
Emulsifier OprG + Terminator B0015
2010-07-12T11:00:00Z
2015-05-08T01:12:22Z
iGEM 2010 Distribution plates
Construction intermediate of emulsifier OprG and stop codon B0015
true
false
_506_
0
6350
9
Discontinued
false
None
false
Pieter van Boheemen
component2073287
1
BBa_B0012
component2073285
1
BBa_B0010
component2073284
1
BBa_K398201
annotation2073287
1
BBa_B0012
range2073287
1
781
821
annotation2073284
1
BBa_K398201
range2073284
1
1
684
annotation2073285
1
BBa_B0010
range2073285
1
693
772
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_K398201
1
BBa_K398201
OprG
2010-06-12T11:00:00Z
2015-05-08T01:12:22Z
NC_002947.3 (base 593454 to 594137)
Outer membrane emulsifying protein from Pseudomonas putida KT2440. Nucleotide sequence optimized for expression in E.coli.
false
false
_506_
0
6320
9
It's complicated
false
None
false
Mathias Voges
annotation2070765
1
stop
range2070765
1
682
684
annotation2070766
1
OrpG
range2070766
1
1
684
annotation2070764
1
start
range2070764
1
1
3
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1686
1
T7 TE
range1686
1
8
27
annotation1690
1
polya
range1690
1
28
41
annotation1687
1
stop
range1687
1
34
34
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K398201_sequence
1
atgaacaaatctctgctgggtgcttctctggttgctctggctctggctgctccggctgctcacgcttaccaggctggtgacatgatcctgcgtgctggtgctatcaccaccgctccgaacgaatcttctggtgacctgaagttcgacggtaacaaagtttctggtaccaaagctaccctggactctgacacccagctgggtctgaccttcgcttacatgctgaccgaccacatcggtctggaactgctggctgctaccccgttcaaacacaccgttggtgttaaaggtctgggtggtggtctggacggtaaactggctgacatcaaacagctgccgccgaccctgtctctgcaatactacccgatggaaccgaactctcgtttccagccgtacgctggtgttggtatcaactacaccctgttcttcgacgaagacctgtcttctgctcgtaaagaacagggtttctctaacctgaaactgcaagactctgttggtatcgctggtcagctgggtatggactacatgctgaccgacaacctgctggttaacgcttctgtttggtacgttgacatcgacaccaaagcttctgttaacggtccgaccgctctgggttactctaaaaccaaagttgacgttgacgttgacccgtgggtttacatggttggtctgggttacaagttctaa
BBa_K398203_sequence
1
atgaacaaatctctgctgggtgcttctctggttgctctggctctggctgctccggctgctcacgcttaccaggctggtgacatgatcctgcgtgctggtgctatcaccaccgctccgaacgaatcttctggtgacctgaagttcgacggtaacaaagtttctggtaccaaagctaccctggactctgacacccagctgggtctgaccttcgcttacatgctgaccgaccacatcggtctggaactgctggctgctaccccgttcaaacacaccgttggtgttaaaggtctgggtggtggtctggacggtaaactggctgacatcaaacagctgccgccgaccctgtctctgcaatactacccgatggaaccgaactctcgtttccagccgtacgctggtgttggtatcaactacaccctgttcttcgacgaagacctgtcttctgctcgtaaagaacagggtttctctaacctgaaactgcaagactctgttggtatcgctggtcagctgggtatggactacatgctgaccgacaacctgctggttaacgcttctgtttggtacgttgacatcgacaccaaagcttctgttaacggtccgaccgctctgggttactctaaaaccaaagttgacgttgacgttgacccgtgggtttacatggttggtctgggttacaagttctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z