BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K398201 1 BBa_K398201 OprG 2010-06-12T11:00:00Z 2015-05-08T01:12:22Z NC_002947.3 (base 593454 to 594137) Outer membrane emulsifying protein from Pseudomonas putida KT2440. Nucleotide sequence optimized for expression in E.coli. false false _506_ 0 6320 9 It's complicated false None false Mathias Voges annotation2070765 1 stop range2070765 1 682 684 annotation2070764 1 start range2070764 1 1 3 annotation2070766 1 OrpG range2070766 1 1 684 BBa_K398203 1 BBa_K398203 Emulsifier OprG + Terminator B0015 2010-07-12T11:00:00Z 2015-05-08T01:12:22Z iGEM 2010 Distribution plates Construction intermediate of emulsifier OprG and stop codon B0015 true false _506_ 0 6350 9 Discontinued false None false Pieter van Boheemen component2073287 1 BBa_B0012 component2073284 1 BBa_K398201 component2073285 1 BBa_B0010 annotation2073285 1 BBa_B0010 range2073285 1 693 772 annotation2073287 1 BBa_B0012 range2073287 1 781 821 annotation2073284 1 BBa_K398201 range2073284 1 1 684 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K398201_sequence 1 atgaacaaatctctgctgggtgcttctctggttgctctggctctggctgctccggctgctcacgcttaccaggctggtgacatgatcctgcgtgctggtgctatcaccaccgctccgaacgaatcttctggtgacctgaagttcgacggtaacaaagtttctggtaccaaagctaccctggactctgacacccagctgggtctgaccttcgcttacatgctgaccgaccacatcggtctggaactgctggctgctaccccgttcaaacacaccgttggtgttaaaggtctgggtggtggtctggacggtaaactggctgacatcaaacagctgccgccgaccctgtctctgcaatactacccgatggaaccgaactctcgtttccagccgtacgctggtgttggtatcaactacaccctgttcttcgacgaagacctgtcttctgctcgtaaagaacagggtttctctaacctgaaactgcaagactctgttggtatcgctggtcagctgggtatggactacatgctgaccgacaacctgctggttaacgcttctgtttggtacgttgacatcgacaccaaagcttctgttaacggtccgaccgctctgggttactctaaaaccaaagttgacgttgacgttgacccgtgggtttacatggttggtctgggttacaagttctaa BBa_K398203_sequence 1 atgaacaaatctctgctgggtgcttctctggttgctctggctctggctgctccggctgctcacgcttaccaggctggtgacatgatcctgcgtgctggtgctatcaccaccgctccgaacgaatcttctggtgacctgaagttcgacggtaacaaagtttctggtaccaaagctaccctggactctgacacccagctgggtctgaccttcgcttacatgctgaccgaccacatcggtctggaactgctggctgctaccccgttcaaacacaccgttggtgttaaaggtctgggtggtggtctggacggtaaactggctgacatcaaacagctgccgccgaccctgtctctgcaatactacccgatggaaccgaactctcgtttccagccgtacgctggtgttggtatcaactacaccctgttcttcgacgaagacctgtcttctgctcgtaaagaacagggtttctctaacctgaaactgcaagactctgttggtatcgctggtcagctgggtatggactacatgctgaccgacaacctgctggttaacgcttctgtttggtacgttgacatcgacaccaaagcttctgttaacggtccgaccgctctgggttactctaaaaccaaagttgacgttgacgttgacccgtgggtttacatggttggtctgggttacaagttctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z