BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_K398204 1 BBa_K398204 Emulsifier AlnA + Terminator B0015 2010-07-12T11:00:00Z 2015-05-08T01:12:22Z DNA synthesis based on P. putida genome and iGEM Distribution plates Construction intermediate of emulsifier AlnA and stop codon B0015 true false _506_ 0 6350 9 Discontinued false None false Pieter van Boheemen component2073688 1 BBa_K398200 component2073689 1 BBa_B0010 component2073691 1 BBa_B0012 annotation2073688 1 BBa_K398200 range2073688 1 1 1047 annotation2073691 1 BBa_B0012 range2073691 1 1144 1184 annotation2073689 1 BBa_B0010 range2073689 1 1056 1135 BBa_K398200 1 BBa_K398200 AlnA 2010-06-12T11:00:00Z 2015-05-08T01:12:22Z Genbank An emulsifying protein present in the Alasan emulsifying complex which naturally occurs in Acinetobacter radioresistens. Nucleotide sequence optimized for expression in E.coli. false false _506_ 0 6320 9 It's complicated false None false Mathias Voges annotation2070763 1 AlnA range2070763 1 1 1047 annotation2070762 1 stop range2070762 1 1045 1047 annotation2070761 1 start range2070761 1 1 3 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K398204_sequence 1 atgaaactgtctcgtatcgctctggctatgctggttgctgctccgctggctgctgctaacgctggtgttaccatcaccccgctgatgctgggttacaccatgttcgacacccagcacaacaacggtggtaacgacggtgacctgaccgactctgttgaactggacgacgacctgttcgttggtgctgctctgggtgttgaactgaccccgtggctcggcttcgaagctgaatactctcagatcaaaggtgacacctctgttgctggtaacgaatacaaaggtgaaaacatcgctggtaacttctacgttacctctgacctgttcaccaaaaactacgactctaaaatcaaaccgtacgctctgctgggtgctggtcactacaaatacgagttcgacaccgacgctggtcgtgctggtcgtggtctggacgaagaaggtaccctgggtaacgctggtctgggtgctttctggcgtatcaacgacgctctgtctctgcgtaccgaagctcgtggtacctaccacttcgacgaacagttctggaactacaccgctctggctggtctgaacgttgttctgggtggtcacctgaaaccggctgctccggttgttgaagttgttccggttgaaccgaccccgatcgttgaaccgcagccgcaggaactgaccgaagacctgaacatggaactgcgtgttttcttcgacaccaacaaatctaacatcaaagaccagtacaaaccggaaatcgctaaagttgctgaaaaactggttgaatacccgaacgctaccgctcgtatcgacggtcacaccgacaacaccggcccgcgtgctctgaacgaacgtctgtctctggctcgtgctaactctgttaaatcttctctggttaacgaatacaacatcgacccgtctcgtctcactgctcagggcttcgcttgggaccagccgatcgctgacaactctaccaaagaaggtcgtgctatgaaccgtcgtgttttcgctaccatcaccggctctcgtaccgttgttgctcagccgggtcaggctcagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K398200_sequence 1 atgaaactgtctcgtatcgctctggctatgctggttgctgctccgctggctgctgctaacgctggtgttaccatcaccccgctgatgctgggttacaccatgttcgacacccagcacaacaacggtggtaacgacggtgacctgaccgactctgttgaactggacgacgacctgttcgttggtgctgctctgggtgttgaactgaccccgtggctcggcttcgaagctgaatactctcagatcaaaggtgacacctctgttgctggtaacgaatacaaaggtgaaaacatcgctggtaacttctacgttacctctgacctgttcaccaaaaactacgactctaaaatcaaaccgtacgctctgctgggtgctggtcactacaaatacgagttcgacaccgacgctggtcgtgctggtcgtggtctggacgaagaaggtaccctgggtaacgctggtctgggtgctttctggcgtatcaacgacgctctgtctctgcgtaccgaagctcgtggtacctaccacttcgacgaacagttctggaactacaccgctctggctggtctgaacgttgttctgggtggtcacctgaaaccggctgctccggttgttgaagttgttccggttgaaccgaccccgatcgttgaaccgcagccgcaggaactgaccgaagacctgaacatggaactgcgtgttttcttcgacaccaacaaatctaacatcaaagaccagtacaaaccggaaatcgctaaagttgctgaaaaactggttgaatacccgaacgctaccgctcgtatcgacggtcacaccgacaacaccggcccgcgtgctctgaacgaacgtctgtctctggctcgtgctaactctgttaaatcttctctggttaacgaatacaacatcgacccgtctcgtctcactgctcagggcttcgcttgggaccagccgatcgctgacaactctaccaaagaaggtcgtgctatgaaccgtcgtgttttcgctaccatcaccggctctcgtaccgttgttgctcagccgggtcaggctcagtaa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z