BBa_K398206
1
BBa_K398206
AlnA generator (IPTG inducible)
2010-10-09T11:00:00Z
2015-05-08T01:12:22Z
Accession no. AY033946
AlnA is a protein with emulsification activity originated from the natural oil degrading bacterium Acinetobacter radioresistens. It has been shown that this protein keeps its emulsifying capacitiesis when it is produced by other organisms, such as Escherichia coli.
AlnA has an amphipathic structure, which allows it to form micelles that accumulate at the interface between liquids of different polarities such as water and oil. This process is based upon the ability of biosurfactants to reduce surface tension, blocking the formation of hydrogen bridges and certain hydrophilic and hydrophobic interactions. The emulsifier helps to disperse the oil by emulsifying the oil, thus increasing the surface area for the growth of microorganisms on hydrocarbons
false
false
_506_
0
6352
9
It's complicated
true
none
false
Eva Brinkman
component2088271
1
BBa_R0011
component2088277
1
BBa_B0032
component2088285
1
BBa_B0012
component2088282
1
BBa_K398200
component2088283
1
BBa_B0010
annotation2088283
1
BBa_B0010
range2088283
1
1138
1217
annotation2088271
1
BBa_R0011
range2088271
1
1
54
annotation2088282
1
BBa_K398200
range2088282
1
83
1129
annotation2088277
1
BBa_B0032
range2088277
1
64
76
annotation2088285
1
BBa_B0012
range2088285
1
1226
1266
BBa_B0032
1
BBa_B0032
RBS.3 (medium) -- derivative of BBa_0030
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>.
false
true
_41_44_48_46_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix ("RBS-2" in figure 4-14 of thesis). <P>
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation1709
1
RBS-3\Weak
range1709
1
1
13
annotation1710
1
RBS
range1710
1
7
10
annotation7027
1
BBa_B0032
range7027
1
1
13
BBa_K398200
1
BBa_K398200
AlnA
2010-06-12T11:00:00Z
2015-05-08T01:12:22Z
Genbank
An emulsifying protein present in the Alasan emulsifying complex which naturally occurs in Acinetobacter radioresistens. Nucleotide sequence optimized for expression in E.coli.
false
false
_506_
0
6320
9
It's complicated
false
None
false
Mathias Voges
annotation2070762
1
stop
range2070762
1
1045
1047
annotation2070763
1
AlnA
range2070763
1
1
1047
annotation2070761
1
start
range2070761
1
1
3
BBa_R0011
1
lacI+pL
Promoter (lacI regulated, lambda pL hybrid)
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
represillator of Elowitz and Leibler (2000)
Released HQ 2013
Inverting regulatory region controlled by LacI (<bb_part>BBa_C0010</bb_part>, <bb_part>BBa_C0011</bb_part>, etc.) <p> The PLlac 0-1 promoter is a hybrid regulatory region consisting of the promoter P(L) of phage lambda with the cI binding sites replaced with lacO1. The hybrid design allows for strong promotion that can nevertheless be tightly repressed by LacI, the Lac inhibitor (i.e. repressor) (<bb_part>BBa_C0010</bb_part>) ([LUTZ97]). The activity of the promoter can be regulated over a >600-fold range by IPTG in E.Coli DH5-alpha-Z1 (same paper reference).
false
true
_1_
0
24
7
In stock
false
<P> <P>hybrid promoter design to create strong promoter that is, at the same time, highly repressible. note that the upstream operator installed in this hybrid is slightly different than the one in the original source (Lutz and Bujard, 1997). the most upstream operator region is slightly truncated in the represillator version, so that both operators in the hybrid are the same sequence. see references for details. also, the sequence has been truncated after the transcriptional start site.<P>LacI binds to this regulator. This part is incompatible with species containing active LacI coding regions. Lactose and IPTG disable the operation of LacI and increase transcription. This part is incompatible with environments containing lactose or lactose analogs.
true
Neelaksh Varshney, Grace Kenney, Daniel Shen, Samantha Sutton
annotation1999
1
lac O1
range1999
1
3
19
annotation2001
1
lac O1
range2001
1
26
42
annotation2002
1
-10
range2002
1
43
48
annotation7064
1
BBa_R0011
range7064
1
1
54
annotation2000
1
-35
range2000
1
20
25
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_K398206_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcacatactagagtcacacaggaaagtactagatgaaactgtctcgtatcgctctggctatgctggttgctgctccgctggctgctgctaacgctggtgttaccatcaccccgctgatgctgggttacaccatgttcgacacccagcacaacaacggtggtaacgacggtgacctgaccgactctgttgaactggacgacgacctgttcgttggtgctgctctgggtgttgaactgaccccgtggctcggcttcgaagctgaatactctcagatcaaaggtgacacctctgttgctggtaacgaatacaaaggtgaaaacatcgctggtaacttctacgttacctctgacctgttcaccaaaaactacgactctaaaatcaaaccgtacgctctgctgggtgctggtcactacaaatacgagttcgacaccgacgctggtcgtgctggtcgtggtctggacgaagaaggtaccctgggtaacgctggtctgggtgctttctggcgtatcaacgacgctctgtctctgcgtaccgaagctcgtggtacctaccacttcgacgaacagttctggaactacaccgctctggctggtctgaacgttgttctgggtggtcacctgaaaccggctgctccggttgttgaagttgttccggttgaaccgaccccgatcgttgaaccgcagccgcaggaactgaccgaagacctgaacatggaactgcgtgttttcttcgacaccaacaaatctaacatcaaagaccagtacaaaccggaaatcgctaaagttgctgaaaaactggttgaatacccgaacgctaccgctcgtatcgacggtcacaccgacaacaccggcccgcgtgctctgaacgaacgtctgtctctggctcgtgctaactctgttaaatcttctctggttaacgaatacaacatcgacccgtctcgtctcactgctcagggcttcgcttgggaccagccgatcgctgacaactctaccaaagaaggtcgtgctatgaaccgtcgtgttttcgctaccatcaccggctctcgtaccgttgttgctcagccgggtcaggctcagtaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0032_sequence
1
tcacacaggaaag
BBa_K398200_sequence
1
atgaaactgtctcgtatcgctctggctatgctggttgctgctccgctggctgctgctaacgctggtgttaccatcaccccgctgatgctgggttacaccatgttcgacacccagcacaacaacggtggtaacgacggtgacctgaccgactctgttgaactggacgacgacctgttcgttggtgctgctctgggtgttgaactgaccccgtggctcggcttcgaagctgaatactctcagatcaaaggtgacacctctgttgctggtaacgaatacaaaggtgaaaacatcgctggtaacttctacgttacctctgacctgttcaccaaaaactacgactctaaaatcaaaccgtacgctctgctgggtgctggtcactacaaatacgagttcgacaccgacgctggtcgtgctggtcgtggtctggacgaagaaggtaccctgggtaacgctggtctgggtgctttctggcgtatcaacgacgctctgtctctgcgtaccgaagctcgtggtacctaccacttcgacgaacagttctggaactacaccgctctggctggtctgaacgttgttctgggtggtcacctgaaaccggctgctccggttgttgaagttgttccggttgaaccgaccccgatcgttgaaccgcagccgcaggaactgaccgaagacctgaacatggaactgcgtgttttcttcgacaccaacaaatctaacatcaaagaccagtacaaaccggaaatcgctaaagttgctgaaaaactggttgaatacccgaacgctaccgctcgtatcgacggtcacaccgacaacaccggcccgcgtgctctgaacgaacgtctgtctctggctcgtgctaactctgttaaatcttctctggttaacgaatacaacatcgacccgtctcgtctcactgctcagggcttcgcttgggaccagccgatcgctgacaactctaccaaagaaggtcgtgctatgaaccgtcgtgttttcgctaccatcaccggctctcgtaccgttgttgctcagccgggtcaggctcagtaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_R0011_sequence
1
aattgtgagcggataacaattgacattgtgagcggataacaagatactgagcaca
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z