LOCUS       BBa_K398305             3533 bp    DNA     linear       22-Nov-2024
DEFINITION  AlkS_E0240
ACCESSION   BBa_K398305
VERSION     BBa_K398305.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start
     misc_feature    2647..2649
                     /label=stop
     CDS             1..2649
                     /label=AlkS
     CDS             1..2649
                     /label=BBa_K398300
     RBS             2658..2670
                     /label=RBS-3\Weak
     RBS             2664..2667
                     /label=RBS
     misc_feature    2658..2670
                     /label=BBa_B0032
     RBS             2658..2670
                     /label=BBa_B0032
     CDS             2677..3396
                     /label=GFP protein
     CDS             2677..3396
                     /label=BBa_E0040
     stem_loop       3416..3459
                     /label=stem_loop
     misc_feature    3405..3484
                     /label=BBa_B0010
     terminator      3405..3484
                     /label=BBa_B0010
     stem_loop       3500..3519
                     /label=T7 TE
     misc_feature    3526..3526
                     /label=stop
     polyA_site      3520..3533
                     /label=polya
     misc_feature    3493..3533
                     /label=BBa_B0012
     terminator      3493..3533
                     /label=BBa_B0012
ORIGIN
        1 atgaaaataa taataaataa cgacttcccg gttgctaaag ttggtgctga ccagataact
       61 accctggtaa gcgctaaagt tcactcttgc atctaccgtc cgcgtctgtc tatcgctgat
      121 ggtgctgctc cgcgtgtctg cctgtacagg gctccgccgg gttacggtaa aaccgttgcg
      181 ctcgcgttcg aatggctgcg tcaccgtacc gcaggtcgtc ccgctgtgtg gctgagcctg
      241 cgtgcttctt cttactctga atttgacatc tgcgctgaaa tcatcgaaca gctggaaacc
      301 ttcgaaatgg ttaaattctc tcgtgttcgt gaaggtgttt ctaaaccggc tctgctgcgt
      361 gacctggctt cttctctgtg gcagtctacc tctaacaacg aaatcgaaac cctggtttgc
      421 ctggacaaca tcaaccacga cctggacctg ccgctgctgc acgctctgat ggaatttatg
      481 ctgaacaccc cgaaaaacat ccgtttcgct gttgctggta acaccataaa gggcttctct
      541 cagctgaagc tggctggtgc tatgcgtgaa tacaccgaaa aagacctggc tttctctgct
      601 gaagaagctg ttgctctggc tgaagctgaa tctgttctgg gtgttccgga agaacagatc
      661 gaaaccctgg ttcaggaagt tgaaggttgg ccggctctgg ttgttttcct gctgaaacgt
      721 gaactgccgg ctaaacacat ctctgctgtt gttgaagttg acaactactt ccgtgacgaa
      781 atcttcgaag ctatcccgga acgttacagg gtattcctgg cgaactcttc tctgctggac
      841 ttcgttaccc cggaccagta caactacgtt ttcaaatgcg ttaacggtgt ttcttgcatc
      901 aaatacctgt ctaccaacta catgctgctg cgtcacgttt ctggtgaacc ggctcagttc
      961 accctgcacc cggttctgcg taacttcctg cgtgaaatca cctggaccga aaacccggct
     1021 aaacgttctt acctgctgaa acgtgctgct ttctggcact ggcgtcgtgg tgaataccag
     1081 tacgctatcc gtatctctct gcgtgctaat gactgccgtt gggctgtgag catgtctgaa
     1141 cgtatcatcc tggacctgtc tttccgtcag ggtgaaatcg acgctctgcg tcagtggctg
     1201 ctggaactgc cgaaacaggc ttggcaccag aaaccgatcg ttctgatctc ttacgcttgg
     1261 gttctgtact tctctcagca gggtgctcgt gctgaaaaac tgatcaaaga cctgtcttct
     1321 cagtctgaca aaaaaaacaa atggcaggaa aaagaatggc tgcaactggt tctggctatc
     1381 ggtaaagcta ccaaagacga aatgctgtct tctgaagaac tgtgcaacaa atggatctct
     1441 ctgttcggtg actctaacgc tgttggtaaa ggtgctgctc tgacctgcct ggctttcatc
     1501 ttcgcttctg aataccgttt cgctgaactg gaaaaagttc tggctcaggc tcaggctgtt
     1561 aacaaattcg ctaaacagaa cttcgctttc ggttggctgt acgttgctcg tttccagcag
     1621 gctctggctt ctggtaaaat gggttgggct cgtcagatca tcacccaggc tcgtaccgac
     1681 tctcgtgctc agatgatgga atctgaattt acctctaaaa tgttcgacgc tctggaactg
     1741 gaactgcact acgaactgcg ttgcctggac acctctgaag aaaaactgtc taaaatcctg
     1801 gaatttatct ctaaccacgg ggtaactgac gtcttcttca gcgtctgtcg tgctgtaagc
     1861 gcttggcgtc tgggtcgttc tgacctgaac ggttctatcg aaatcctgga atgggctaaa
     1921 gctcacgctg ttgaaaaaaa cctgccgcgt ctggaagtta tgtctcagat cgaaatctac
     1981 caacgtctgg tatgccaggg aatcaccggt atcaacaacc tgaaaaccct ggaagaccac
     2041 aaaatcttct ctggtcagca ctctgctccg ctgaaagctc gtctgctgct ggttcagtct
     2101 ctggttctgt ctcgtgaccg taacttccac tctgctgctc accgtgctct gctggctatc
     2161 cagcaggctc gtaaaatcaa cgctggtcag ctggaagttc gtggtctgct gtgcctggct
     2221 ggtgctcagg ctggtgctgg tgacctgaaa aaagctcagc tgaacatcgt ttacgctgtt
     2281 gaaatcgcta aacagctgca atgcttccag accgttctcg acgaagtctg cctgatcgag
     2341 cgtatcatcc cggctagttg cgaagcgttc accgctgtta atctggacca ggctatcggt
     2401 gctttctctc tgccgcgtat cgttgaaatc ggtaaatctg ctgaaaacaa agctgacgct
     2461 ctgctgaccc gtaaacagat cgctgttctg cgtctggtta aagaaggttg ctctaacaaa
     2521 cagatcgcta ccaacatgca cgttaccgaa gacgctatca aatggcacat gcgtaaaatc
     2581 ttcgctaccc tgaacgttgt taaccgtacc caggctacca tcgaagctga acgtcagggt
     2641 atcatctaat actagagtca cacaggaaag tactagatgc gtaaaggaga agaacttttc
     2701 actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     2761 gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     2821 actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt
     2881 caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg
     2941 cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     3001 cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     3061 gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     3121 aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
     3181 cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     3241 ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     3301 aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     3361 attacacatg gcatggatga actatacaaa taataatact agagccaggc atcaaataaa
     3421 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     3481 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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