BBa_K398305 1 BBa_K398305 AlkS_E0240 2010-10-09T11:00:00Z 2015-05-08T01:12:22Z Genbank AlkS transcriptional regulator activates the expression of its own gene, and that of alkT, from a promoter named PalkS2 in the presence of alkanes. . AlkS levels that are high enough to activate the expression of the alkBFGHJKL operon from the PalkB promoter. AlkS recognizes C5???C10 n-alkanes as effectors, but does not respond to shorter or larger alkanes. false false _506_ 0 6352 9 It's complicated false Nucleotide sequence optimized for expression in E.coli false Eva Brinkman component2084773 1 BBa_B0012 component2084770 1 BBa_E0040 component2084765 1 BBa_K398300 component2084767 1 BBa_B0032 component2084771 1 BBa_B0010 annotation2084767 1 BBa_B0032 range2084767 1 2658 2670 annotation2084771 1 BBa_B0010 range2084771 1 3405 3484 annotation2084773 1 BBa_B0012 range2084773 1 3493 3533 annotation2084765 1 BBa_K398300 range2084765 1 1 2649 annotation2084770 1 BBa_E0040 range2084770 1 2677 3396 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 BBa_K398300 1 BBa_K398300 AlkS 2010-06-13T11:00:00Z 2015-05-08T01:12:22Z Genbank Transcription factor involved in the expression of alkane-degrading genes in Pseudomonas putida OCT. false false _506_ 0 6320 9 It's complicated false None false Mathias Voges annotation2070768 1 stop range2070768 1 2647 2649 annotation2070767 1 start range2070767 1 1 3 annotation2070769 1 AlkS range2070769 1 1 2649 BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1710 1 RBS range1710 1 7 10 annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation7027 1 BBa_B0032 range7027 1 1 13 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation4184 1 stem_loop range4184 1 12 55 annotation7018 1 BBa_B0010 range7018 1 1 80 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K398305_sequence 1 atgaaaataataataaataacgacttcccggttgctaaagttggtgctgaccagataactaccctggtaagcgctaaagttcactcttgcatctaccgtccgcgtctgtctatcgctgatggtgctgctccgcgtgtctgcctgtacagggctccgccgggttacggtaaaaccgttgcgctcgcgttcgaatggctgcgtcaccgtaccgcaggtcgtcccgctgtgtggctgagcctgcgtgcttcttcttactctgaatttgacatctgcgctgaaatcatcgaacagctggaaaccttcgaaatggttaaattctctcgtgttcgtgaaggtgtttctaaaccggctctgctgcgtgacctggcttcttctctgtggcagtctacctctaacaacgaaatcgaaaccctggtttgcctggacaacatcaaccacgacctggacctgccgctgctgcacgctctgatggaatttatgctgaacaccccgaaaaacatccgtttcgctgttgctggtaacaccataaagggcttctctcagctgaagctggctggtgctatgcgtgaatacaccgaaaaagacctggctttctctgctgaagaagctgttgctctggctgaagctgaatctgttctgggtgttccggaagaacagatcgaaaccctggttcaggaagttgaaggttggccggctctggttgttttcctgctgaaacgtgaactgccggctaaacacatctctgctgttgttgaagttgacaactacttccgtgacgaaatcttcgaagctatcccggaacgttacagggtattcctggcgaactcttctctgctggacttcgttaccccggaccagtacaactacgttttcaaatgcgttaacggtgtttcttgcatcaaatacctgtctaccaactacatgctgctgcgtcacgtttctggtgaaccggctcagttcaccctgcacccggttctgcgtaacttcctgcgtgaaatcacctggaccgaaaacccggctaaacgttcttacctgctgaaacgtgctgctttctggcactggcgtcgtggtgaataccagtacgctatccgtatctctctgcgtgctaatgactgccgttgggctgtgagcatgtctgaacgtatcatcctggacctgtctttccgtcagggtgaaatcgacgctctgcgtcagtggctgctggaactgccgaaacaggcttggcaccagaaaccgatcgttctgatctcttacgcttgggttctgtacttctctcagcagggtgctcgtgctgaaaaactgatcaaagacctgtcttctcagtctgacaaaaaaaacaaatggcaggaaaaagaatggctgcaactggttctggctatcggtaaagctaccaaagacgaaatgctgtcttctgaagaactgtgcaacaaatggatctctctgttcggtgactctaacgctgttggtaaaggtgctgctctgacctgcctggctttcatcttcgcttctgaataccgtttcgctgaactggaaaaagttctggctcaggctcaggctgttaacaaattcgctaaacagaacttcgctttcggttggctgtacgttgctcgtttccagcaggctctggcttctggtaaaatgggttgggctcgtcagatcatcacccaggctcgtaccgactctcgtgctcagatgatggaatctgaatttacctctaaaatgttcgacgctctggaactggaactgcactacgaactgcgttgcctggacacctctgaagaaaaactgtctaaaatcctggaatttatctctaaccacggggtaactgacgtcttcttcagcgtctgtcgtgctgtaagcgcttggcgtctgggtcgttctgacctgaacggttctatcgaaatcctggaatgggctaaagctcacgctgttgaaaaaaacctgccgcgtctggaagttatgtctcagatcgaaatctaccaacgtctggtatgccagggaatcaccggtatcaacaacctgaaaaccctggaagaccacaaaatcttctctggtcagcactctgctccgctgaaagctcgtctgctgctggttcagtctctggttctgtctcgtgaccgtaacttccactctgctgctcaccgtgctctgctggctatccagcaggctcgtaaaatcaacgctggtcagctggaagttcgtggtctgctgtgcctggctggtgctcaggctggtgctggtgacctgaaaaaagctcagctgaacatcgtttacgctgttgaaatcgctaaacagctgcaatgcttccagaccgttctcgacgaagtctgcctgatcgagcgtatcatcccggctagttgcgaagcgttcaccgctgttaatctggaccaggctatcggtgctttctctctgccgcgtatcgttgaaatcggtaaatctgctgaaaacaaagctgacgctctgctgacccgtaaacagatcgctgttctgcgtctggttaaagaaggttgctctaacaaacagatcgctaccaacatgcacgttaccgaagacgctatcaaatggcacatgcgtaaaatcttcgctaccctgaacgttgttaaccgtacccaggctaccatcgaagctgaacgtcagggtatcatctaatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0032_sequence 1 tcacacaggaaag BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K398300_sequence 1 atgaaaataataataaataacgacttcccggttgctaaagttggtgctgaccagataactaccctggtaagcgctaaagttcactcttgcatctaccgtccgcgtctgtctatcgctgatggtgctgctccgcgtgtctgcctgtacagggctccgccgggttacggtaaaaccgttgcgctcgcgttcgaatggctgcgtcaccgtaccgcaggtcgtcccgctgtgtggctgagcctgcgtgcttcttcttactctgaatttgacatctgcgctgaaatcatcgaacagctggaaaccttcgaaatggttaaattctctcgtgttcgtgaaggtgtttctaaaccggctctgctgcgtgacctggcttcttctctgtggcagtctacctctaacaacgaaatcgaaaccctggtttgcctggacaacatcaaccacgacctggacctgccgctgctgcacgctctgatggaatttatgctgaacaccccgaaaaacatccgtttcgctgttgctggtaacaccataaagggcttctctcagctgaagctggctggtgctatgcgtgaatacaccgaaaaagacctggctttctctgctgaagaagctgttgctctggctgaagctgaatctgttctgggtgttccggaagaacagatcgaaaccctggttcaggaagttgaaggttggccggctctggttgttttcctgctgaaacgtgaactgccggctaaacacatctctgctgttgttgaagttgacaactacttccgtgacgaaatcttcgaagctatcccggaacgttacagggtattcctggcgaactcttctctgctggacttcgttaccccggaccagtacaactacgttttcaaatgcgttaacggtgtttcttgcatcaaatacctgtctaccaactacatgctgctgcgtcacgtttctggtgaaccggctcagttcaccctgcacccggttctgcgtaacttcctgcgtgaaatcacctggaccgaaaacccggctaaacgttcttacctgctgaaacgtgctgctttctggcactggcgtcgtggtgaataccagtacgctatccgtatctctctgcgtgctaatgactgccgttgggctgtgagcatgtctgaacgtatcatcctggacctgtctttccgtcagggtgaaatcgacgctctgcgtcagtggctgctggaactgccgaaacaggcttggcaccagaaaccgatcgttctgatctcttacgcttgggttctgtacttctctcagcagggtgctcgtgctgaaaaactgatcaaagacctgtcttctcagtctgacaaaaaaaacaaatggcaggaaaaagaatggctgcaactggttctggctatcggtaaagctaccaaagacgaaatgctgtcttctgaagaactgtgcaacaaatggatctctctgttcggtgactctaacgctgttggtaaaggtgctgctctgacctgcctggctttcatcttcgcttctgaataccgtttcgctgaactggaaaaagttctggctcaggctcaggctgttaacaaattcgctaaacagaacttcgctttcggttggctgtacgttgctcgtttccagcaggctctggcttctggtaaaatgggttgggctcgtcagatcatcacccaggctcgtaccgactctcgtgctcagatgatggaatctgaatttacctctaaaatgttcgacgctctggaactggaactgcactacgaactgcgttgcctggacacctctgaagaaaaactgtctaaaatcctggaatttatctctaaccacggggtaactgacgtcttcttcagcgtctgtcgtgctgtaagcgcttggcgtctgggtcgttctgacctgaacggttctatcgaaatcctggaatgggctaaagctcacgctgttgaaaaaaacctgccgcgtctggaagttatgtctcagatcgaaatctaccaacgtctggtatgccagggaatcaccggtatcaacaacctgaaaaccctggaagaccacaaaatcttctctggtcagcactctgctccgctgaaagctcgtctgctgctggttcagtctctggttctgtctcgtgaccgtaacttccactctgctgctcaccgtgctctgctggctatccagcaggctcgtaaaatcaacgctggtcagctggaagttcgtggtctgctgtgcctggctggtgctcaggctggtgctggtgacctgaaaaaagctcagctgaacatcgtttacgctgttgaaatcgctaaacagctgcaatgcttccagaccgttctcgacgaagtctgcctgatcgagcgtatcatcccggctagttgcgaagcgttcaccgctgttaatctggaccaggctatcggtgctttctctctgccgcgtatcgttgaaatcggtaaatctgctgaaaacaaagctgacgctctgctgacccgtaaacagatcgctgttctgcgtctggttaaagaaggttgctctaacaaacagatcgctaccaacatgcacgttaccgaagacgctatcaaatggcacatgcgtaaaatcttcgctaccctgaacgttgttaaccgtacccaggctaccatcgaagctgaacgtcagggtatcatctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z