BBa_K398406 1 406 Solvent tolerance cluster 2010-10-11T11:00:00Z 2015-05-08T01:12:23Z Genomic sequence obtained from NCBI, the sequences were enhanced for iGEM purposes Four subunits alpha and two subunits beta create a chaperone that confers solvent tolerance to E. coli K12 false false _506_ 0 6321 9 It's complicated true Enhanced sequence for expression on E. coli K12, restriction sites for the enzymes XbaI, PstI, EcRI and SpeI are removed from the CDS. An extra XbaI site will be found between RBS and Promoter so you can increase the expression level of the protein by changing the promoter. false Hugo Federico Cueto Rojas and Ramon van der Valk annotation2085527 1 BBa_J61107 range2085527 1 44 55 annotation2085529 1 PhPFD-alpha range2085529 1 62 517 annotation2085533 1 PhPFD-beta range2085533 1 544 897 annotation2085530 1 stop range2085530 1 515 517 annotation2085534 1 start range2085534 1 544 546 annotation2085536 1 BBa_K398401 range2085536 1 544 897 annotation2085535 1 stop range2085535 1 895 897 annotation2085531 1 BBa_K398400 range2085531 1 62 517 annotation2085532 1 BBa_J61107 range2085532 1 526 537 annotation2085528 1 start range2085528 1 62 64 annotation2085526 1 BBa_J23100 range2085526 1 1 35 annotation2085537 1 XbaI site range2085537 1 36 41 BBa_K398406_sequence 1 ttgacggctagctcagtcctaggtacagtgctagcttctagagaaagaagagacttactagatgatccgtatggctcagaacaacaaagaactggaaaaactggcttacgaataccaggttctgcaagctcaggctcagatcctggctcagaacctggaactgctgaacctggctaaagctgaagttcagaccgttcgtgaaaccctggaaaacctgaaaaaaatcgaagaagaaaaaccggaaatcctggttccgatcggtgctggttctttcctgaaaggtgttatcgttgacaaaaacaacgctatcgtttctgttggttctggttacgctgttgaacgttctatcgacgaagctatctctttcctggaaaaacgtctgaaagaatacgacgaagctatcaaaaaaacccagggtgctctggctgaactggaaaaacgtatcggtgaagttgctcgtaaagctcaggaagttcagcagaaacagtctatgacctctttcaaagttaaaaaataatactagagaaagaagagacttactagatgcagaacatcccgccgcaggttcaggctatgctgggtcagctggacacctaccagcagcagctgcaactggttatccagcagaaacagaaagttcaggctgacctgaacgaagctaaaaaagctctggaagaaatcgaaaccctgccggacgacgctcagatatacaaaaccgttggtaccctgatcgttaaaaccaccaaagaaaaagctgttcaggaactgaaagaaaaaatcgaaaccctggaagttcgtctgaacgctctgaaccgtcaggaacagaaaatcaacgaaaaagttaaagaactgacccagaaaatccaggctgctctgcgtccgccgaccgctggttaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z