LOCUS BBa_K400000 5505 bp DNA linear 23-Dec-2024 DEFINITION composite heavy metal-detector(Zn, Cd, As) ACCESSION BBa_K400000 VERSION BBa_K400000.1 FEATURES Location/Qualifiers CDS 1..720 /label=GFP protein misc_feature 1..720 /label=BBa_E0040 misc_feature 729..928 /label=BBa_R0010 misc_feature 817..854 /label=CAP promoter 865..870 /label=-35 promoter 889..894 /label=-10 misc_feature 894..928 /label=LacI misc_feature 901..901 /label=start misc_feature 937..948 /label=BBa_B0034 misc_feature 941..944 /label=conserved misc_feature 955..1635 /label=BBa_E1010 CDS 955..1629 /label=mrfp1 misc_feature 1636..1660 /label=barcode misc_feature 1669..1748 /label=BBa_B0010 stem_loop 1680..1723 /label=stem_loop misc_feature 1757..1797 /label=BBa_B0012 stem_loop 1764..1783 /label=T7 TE polyA_site 1784..1797 /label=polya misc_feature 1790..1790 /label=stop misc_feature 729..1797 /label=BBa_J04450 CDS 1804..3297 /label=mutated aryl acylamidase misc_feature 1804..3297 /label=BBa_K400628 ORIGIN 1 aaacgggcaa ggtgtcacca ccctgccctt tttctttaaa accgaaaaga ttacttcgcg 61 ttatgcaggc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 121 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 181 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 241 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 301 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 361 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 421 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 481 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 541 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 601 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 661 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 721 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 781 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 841 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 901 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 961 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 1021 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 1081 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 1141 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 1201 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 1261 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta 1321 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 1381 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 1441 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 1501 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 1561 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 1621 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 1681 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 1741 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 1801 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 1861 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 1921 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 1981 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 2041 ggcgtatcac gaggcagaat ttcagataaa aaaaatcctt agctttcgct aaggatgatt 2101 tctggaattc cttcatattg ccgacaaagt acgcgttatc cctgccggat gtggcgcgag 2161 cgccctatcc ggcatacaca gatcgcatca ggttatgttt cgtttgtcat caacctcaaa 2221 tcaccaaaat aatttatttt atttcaataa gatagataag tagaactgag aaagccatgc 2281 tctcgtttcc taagagttgt tgcattttgc tatatgttac aatataacat tacacatcat 2341 atacattaac tctggaggaa actgttatgg gtaagtcaca ttcgccagtt cactggaagt 2401 cggcggccga gatcgtcgag ttagtcaaga gcaaacaaat ttccccacgc gaggtcgtgg 2461 aaagcacgat cgacctcatc gagcaacgcg atcccggcct gaatgcggtc gtgtacaagg 2521 cttacgatga agcgcgcgaa aaagcggctg cgcttgagcg gcgcatcatg caaggcgagc 2581 ctgttggcat gctggcgggg gttccgactc tcatgaagga cttgttcgcg gccaagccgg 2641 gctggccgtc cacgctgggc ggcattcgcg ccctcaagga cgcccgcggc gctgccggcg 2701 tctggtccac ttatccattg aagatgtccg gcgaagacag cctgttgctg ggacagacaa 2761 acagccccgt ctatggcttt cgcggaacca cggacaacac gtttttcggt ccgacgcgca 2821 atccattcaa cctcgacttc aacgcagggg ggtcttcggg aggagccgct gcgctcgtgg 2881 ccgatggcat cgttcctgtg gcaggcggca cggatggcgg cggctcgatc cgcattccgg 2941 cggcatggac caatacctac ggctttcagc cttcgatagg acgcgttccg ttcaaaagcc 3001 cgccccaatg ctttccatcc ggggaccgta tctctatgaa ggagcccatc acgcggacgg 3061 tgccggatgc ggcattggca atgaatgtac tgcacggctt cgaccgccgc gatcctgctt 3121 cgctgcgagt caagctggac ttcacctcag cgcttgcaca aggcgtgcgt ggcaaaaaaa 3181 tcggcctgac gctgaattac ggggtatttc ccgtccagca agagattcag gatttgatcg 3241 gcaaggctgc ccgggttttt acagagcttg gcgcacacgt cgagttcgtc gacctcggta 3301 tcccctacag ccagaaacaa atgagcgatg cctggtgccg catgatcgcg attcctactg 3361 tcgcctcgat gcaggcgctg cgcaaggaag gcattgactt atatggcgaa catcgggcag 3421 acatacccga tgcgctgatg aagtggatag atgcggtcgc tgacataagc gtccagcaga 3481 tcagcgctga tcaactattg agaacgaccg ttttcgattg catgaacggc gtattcgatc 3541 gctttgacct attattggcg ccgaccctgg catgcatgcc tgttcgcaat gcgactgatg 3601 gctgcaccga gggaccaagc cagatcaacg gtgaagaaat cgaccctttg atcggctggt 3661 gcatgactta cctgaccaat ttttcagggc atcccagtgc cagtgtcccg gctggcctga 3721 ttgatggcct gccggcaggc atgctcatta tcggcgatcg ccaggcggac ctggatgtca 3781 ttgccgcaag cgccgctttc gagcgagcca gcccgtggtc ccaatactat gacattcccg 3841 ccggacggcc cctgtgataa tctagtcgtc cgctcgctgt atctctgata aaacttgact 3901 ctggagtcga ctccagagtg tatccttcgg ttaatgagaa aaaacttaac cggaggatgc 3961 catggcttcc tccgaagacg ttatcaaaga gttcatgcgt ttcaaagttc gtatggaagg 4021 ttccgttaac ggtcacgagt tcgaaatcga aggtgaaggt gaaggtcgtc cgtacgaagg 4081 tacccagacc gctaaactga aagttaccaa aggtggtccg ctgccgttcg cttgggacat 4141 cctgtccccg cagttccagt acggttccaa agcttacgtt aaacacccgg ctgacatccc 4201 ggactacctg aaactgtcct tcccggaagg tttcaaatgg gaacgtgtta tgaacttcga 4261 agacggtggt gttgttaccg ttacccagga ctcctccctg caagacggtg agttcatcta 4321 caaagttaaa ctgcgtggta ccaacttccc gtccgacggt ccggttatgc agaaaaaaac 4381 catgggttgg gaagcttcca ccgaacgtat gtacccggaa gacggtgctc tgaaaggtga 4441 aatcaaaatg cgtctgaaac tgaaagacgg tggtcactac gacgctgaag ttaaaaccac 4501 ctacatggct aaaaaaccgg ttcagctgcc gggtgcttac aaaaccgaca tcaaactgga 4561 catcacctcc cacaacgaag actacaccat cgttgaacag tacgaacgtg ctgaaggtcg 4621 tcactccacc ggtgcttaat aaactagacc aactcaaaat tcacacctat taccttcctc 4681 tgcacttaca cattcgttaa gtcatatatg tttttgactt atccgcttcg aagagagaca 4741 ctacctgcaa caatcaggag cgcaatatgc gtaaaggaga agaacttttc actggagttg 4801 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag 4861 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa 4921 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg 4981 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt 5041 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag 5101 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag 5161 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca 5221 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg 5281 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc 5341 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca 5401 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg 5461 gcatggatga actatacaaa aggccttaac tgcagtccgg caaaa //