BBa_K400000
1
BBa_K400000
composite heavy metal-detector(Zn, Cd, As)
2010-10-20T11:00:00Z
2015-05-08T01:12:23Z
a soil bacterium
It is a composite part of heavy metal-detecting promoters and reporter genes.
----PyodA-mAAA(BBa_K400628)----PzntA-RFP(BBa_E0040)----ParsR-GFP(BBa_J04450)----
It detects cadmium, zinc, and arsenic in the media and gives brown, red, and green color.
This is our final product to put in a capsule in order to be customized.
false
false
_511_
0
6409
9
It's complicated
false
Each of the parts were cleaved with Endonuclease and ligated with pSB1C3 vector.
First, a promoter and a reporter gene were amplified by PCR and two of the pairs were ligated with pSB1C3 vector.
Second, another promoter and reporter gene were put together by PCR and then ligated with pSB1C3 vector with the other two-pair-fragment.
Three pairs were finally put in a vector and it underwent point mutation during the procedure because it had multiple cleavage sites.
false
Jonghwa Kim
annotation2091120
1
polya
range2091120
1
1784
1797
annotation2091112
1
conserved
range2091112
1
941
944
annotation2091121
1
stop
range2091121
1
1790
1790
annotation2091117
1
stem_loop
range2091117
1
1680
1723
annotation2091105
1
BBa_R0010
range2091105
1
729
928
annotation2091122
1
BBa_J04450
range2091122
1
729
1797
annotation2091109
1
LacI
range2091109
1
894
928
annotation2091107
1
-35
range2091107
1
865
870
annotation2091103
1
GFP protein
range2091103
1
1
720
annotation2091124
1
BBa_K400628
range2091124
1
1804
3297
annotation2091116
1
BBa_B0010
range2091116
1
1669
1748
annotation2091114
1
mrfp1
range2091114
1
955
1629
annotation2091118
1
BBa_B0012
range2091118
1
1757
1797
annotation2091111
1
BBa_B0034
range2091111
1
937
948
annotation2091108
1
-10
range2091108
1
889
894
annotation2091115
1
barcode
range2091115
1
1636
1660
annotation2091113
1
BBa_E1010
range2091113
1
955
1635
annotation2091123
1
mutated aryl acylamidase
range2091123
1
1804
3297
annotation2091110
1
start
range2091110
1
901
901
annotation2091119
1
T7 TE
range2091119
1
1764
1783
annotation2091104
1
BBa_E0040
range2091104
1
1
720
annotation2091106
1
CAP
range2091106
1
817
854
BBa_K400000_sequence
1
aaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggcagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattccttcatattgccgacaaagtacgcgttatccctgccggatgtggcgcgagcgccctatccggcatacacagatcgcatcaggttatgtttcgtttgtcatcaacctcaaatcaccaaaataatttattttatttcaataagatagataagtagaactgagaaagccatgctctcgtttcctaagagttgttgcattttgctatatgttacaatataacattacacatcatatacattaactctggaggaaactgttatgggtaagtcacattcgccagttcactggaagtcggcggccgagatcgtcgagttagtcaagagcaaacaaatttccccacgcgaggtcgtggaaagcacgatcgacctcatcgagcaacgcgatcccggcctgaatgcggtcgtgtacaaggcttacgatgaagcgcgcgaaaaagcggctgcgcttgagcggcgcatcatgcaaggcgagcctgttggcatgctggcgggggttccgactctcatgaaggacttgttcgcggccaagccgggctggccgtccacgctgggcggcattcgcgccctcaaggacgcccgcggcgctgccggcgtctggtccacttatccattgaagatgtccggcgaagacagcctgttgctgggacagacaaacagccccgtctatggctttcgcggaaccacggacaacacgtttttcggtccgacgcgcaatccattcaacctcgacttcaacgcaggggggtcttcgggaggagccgctgcgctcgtggccgatggcatcgttcctgtggcaggcggcacggatggcggcggctcgatccgcattccggcggcatggaccaatacctacggctttcagccttcgataggacgcgttccgttcaaaagcccgccccaatgctttccatccggggaccgtatctctatgaaggagcccatcacgcggacggtgccggatgcggcattggcaatgaatgtactgcacggcttcgaccgccgcgatcctgcttcgctgcgagtcaagctggacttcacctcagcgcttgcacaaggcgtgcgtggcaaaaaaatcggcctgacgctgaattacggggtatttcccgtccagcaagagattcaggatttgatcggcaaggctgcccgggtttttacagagcttggcgcacacgtcgagttcgtcgacctcggtatcccctacagccagaaacaaatgagcgatgcctggtgccgcatgatcgcgattcctactgtcgcctcgatgcaggcgctgcgcaaggaaggcattgacttatatggcgaacatcgggcagacatacccgatgcgctgatgaagtggatagatgcggtcgctgacataagcgtccagcagatcagcgctgatcaactattgagaacgaccgttttcgattgcatgaacggcgtattcgatcgctttgacctattattggcgccgaccctggcatgcatgcctgttcgcaatgcgactgatggctgcaccgagggaccaagccagatcaacggtgaagaaatcgaccctttgatcggctggtgcatgacttacctgaccaatttttcagggcatcccagtgccagtgtcccggctggcctgattgatggcctgccggcaggcatgctcattatcggcgatcgccaggcggacctggatgtcattgccgcaagcgccgctttcgagcgagccagcccgtggtcccaatactatgacattcccgccggacggcccctgtgataatctagtcgtccgctcgctgtatctctgataaaacttgactctggagtcgactccagagtgtatccttcggttaatgagaaaaaacttaaccggaggatgccatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaactagaccaactcaaaattcacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagacactacctgcaacaatcaggagcgcaatatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggccttaactgcagtccggcaaaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z