##gff-version 3
##sequence-region BBa_K400000 1 3297
BBa_K400000	.	promoter	865	870	.	+	0	ID=annotation2091107;Name=-35
BBa_K400000	.	engineered_tag	1636	1660	.	+	0	ID=annotation2091115;Name=barcode
BBa_K400000	.	CDS	1	720	.	+	0	ID=annotation2091103;Name=GFP protein
BBa_K400000	.	conserved_region	941	944	.	+	0	ID=annotation2091112;Name=conserved
BBa_K400000	.	start_codon	901	901	.	+	0	ID=annotation2091110;Name=start
BBa_K400000	.	engineered_region	1804	3297	.	+	0	ID=annotation2091124;Name=BBa_K400628
BBa_K400000	.	engineered_region	729	928	.	+	0	ID=annotation2091105;Name=BBa_R0010
BBa_K400000	.	CDS	1804	3297	.	+	0	ID=annotation2091123;Name=mutated aryl acylamidase
BBa_K400000	.	non_covalent_binding_site	894	928	.	+	0	ID=annotation2091109;Name=LacI
BBa_K400000	.	CDS	955	1629	.	+	0	ID=annotation2091114;Name=mrfp1
BBa_K400000	.	stop_codon	1790	1790	.	+	0	ID=annotation2091121;Name=stop
BBa_K400000	.	engineered_region	955	1635	.	+	0	ID=annotation2091113;Name=BBa_E1010
BBa_K400000	.	polyA_site	1784	1797	.	+	0	ID=annotation2091120;Name=polya
BBa_K400000	.	non_covalent_binding_site	817	854	.	+	0	ID=annotation2091106;Name=CAP
BBa_K400000	.	engineered_region	937	948	.	+	0	ID=annotation2091111;Name=BBa_B0034
BBa_K400000	.	engineered_region	729	1797	.	+	0	ID=annotation2091122;Name=BBa_J04450
BBa_K400000	.	engineered_region	1757	1797	.	+	0	ID=annotation2091118;Name=BBa_B0012
BBa_K400000	.	engineered_region	1669	1748	.	+	0	ID=annotation2091116;Name=BBa_B0010
BBa_K400000	.	stem_loop	1764	1783	.	+	0	ID=annotation2091119;Name=T7 TE
BBa_K400000	.	promoter	889	894	.	+	0	ID=annotation2091108;Name=-10
BBa_K400000	.	stem_loop	1680	1723	.	+	0	ID=annotation2091117;Name=stem_loop
BBa_K400000	.	engineered_region	1	720	.	+	0	ID=annotation2091104;Name=BBa_E0040
>BBa_K400000
aaacgggcaaggtgtcaccaccctgccctttttctttaaaaccgaaaagattacttcgcgttatgcaggcttcctcgct
cactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccaca
gaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgc
tggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgac
aggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccgga
tacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtagg
tcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtct
tgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgta
ggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgc
tgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttt
tgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgct
cagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatt
aaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggc
acctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggag
ggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaacc
agccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccggga
agctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcg
tcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaag
cggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcact
gcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaa
tagtgtatgcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcagaactttaaaag
tgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacc
cactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaat
gccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcattt
atcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatt
tccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgagg
cagaatttcagataaaaaaaatccttagctttcgctaaggatgatttctggaattccttcatattgccgacaaagtacg
cgttatccctgccggatgtggcgcgagcgccctatccggcatacacagatcgcatcaggttatgtttcgtttgtcatca
acctcaaatcaccaaaataatttattttatttcaataagatagataagtagaactgagaaagccatgctctcgtttcct
aagagttgttgcattttgctatatgttacaatataacattacacatcatatacattaactctggaggaaactgttatgg
gtaagtcacattcgccagttcactggaagtcggcggccgagatcgtcgagttagtcaagagcaaacaaatttccccacg
cgaggtcgtggaaagcacgatcgacctcatcgagcaacgcgatcccggcctgaatgcggtcgtgtacaaggcttacgat
gaagcgcgcgaaaaagcggctgcgcttgagcggcgcatcatgcaaggcgagcctgttggcatgctggcgggggttccga
ctctcatgaaggacttgttcgcggccaagccgggctggccgtccacgctgggcggcattcgcgccctcaaggacgcccg
cggcgctgccggcgtctggtccacttatccattgaagatgtccggcgaagacagcctgttgctgggacagacaaacagc
cccgtctatggctttcgcggaaccacggacaacacgtttttcggtccgacgcgcaatccattcaacctcgacttcaacg
caggggggtcttcgggaggagccgctgcgctcgtggccgatggcatcgttcctgtggcaggcggcacggatggcggcgg
ctcgatccgcattccggcggcatggaccaatacctacggctttcagccttcgataggacgcgttccgttcaaaagcccg
ccccaatgctttccatccggggaccgtatctctatgaaggagcccatcacgcggacggtgccggatgcggcattggcaa
tgaatgtactgcacggcttcgaccgccgcgatcctgcttcgctgcgagtcaagctggacttcacctcagcgcttgcaca
aggcgtgcgtggcaaaaaaatcggcctgacgctgaattacggggtatttcccgtccagcaagagattcaggatttgatc
ggcaaggctgcccgggtttttacagagcttggcgcacacgtcgagttcgtcgacctcggtatcccctacagccagaaac
aaatgagcgatgcctggtgccgcatgatcgcgattcctactgtcgcctcgatgcaggcgctgcgcaaggaaggcattga
cttatatggcgaacatcgggcagacatacccgatgcgctgatgaagtggatagatgcggtcgctgacataagcgtccag
cagatcagcgctgatcaactattgagaacgaccgttttcgattgcatgaacggcgtattcgatcgctttgacctattat
tggcgccgaccctggcatgcatgcctgttcgcaatgcgactgatggctgcaccgagggaccaagccagatcaacggtga
agaaatcgaccctttgatcggctggtgcatgacttacctgaccaatttttcagggcatcccagtgccagtgtcccggct
ggcctgattgatggcctgccggcaggcatgctcattatcggcgatcgccaggcggacctggatgtcattgccgcaagcg
ccgctttcgagcgagccagcccgtggtcccaatactatgacattcccgccggacggcccctgtgataatctagtcgtcc
gctcgctgtatctctgataaaacttgactctggagtcgactccagagtgtatccttcggttaatgagaaaaaacttaac
cggaggatgccatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaa
cggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttacc
aaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacc
cggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacgg
tggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaac
ttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacg
gtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccaccta
catggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagac
tacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataaactagaccaactcaaaatt
cacacctattaccttcctctgcacttacacattcgttaagtcatatatgtttttgacttatccgcttcgaagagagaca
ctacctgcaacaatcaggagcgcaatatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaatt
agatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttaccctt
aaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgct
ttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaac
tatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatc
gagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatg
tatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgt
tcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcc
acacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctggga
ttacacatggcatggatgaactatacaaaaggccttaactgcagtccggcaaaa