BBa_K400628
1
BBa_K400628
mAAA (EcoRI site mutated Aryl Acyl-Amidase)
2010-10-18T11:00:00Z
2015-05-08T01:12:23Z
It was identified from a genomic library of a soil bacterium, which was isolated using a selection media having p-acetaminophenol as a sole carbon source.
Aryl acylamidase is an enzyme that acts in the amide bond between aryl- and acyl- compounds.
The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is reversible.
When a p-acetaminophenol was reacted with AAA, the product gave a purple-red color.
As a result, a clone presenting AAA activity from the genomic library gave a color change around the colony when it was grown on solid media enriched with p-acetaminophenol.
Since it gives color to colorless substance, it can be used as a reporter gene to a specific promoter.
In our project, we put it downstream of cadmium-dependent promoter to detect any cadmium present in a solution.
false
false
_511_
0
6640
9
It's complicated
false
This part was cloned into pSB1C3 vector and mAAA was mutated in two EcoRI sites.
false
Seobeom Heo
annotation2089461
1
mutated aryl acylamidase
range2089461
1
1
1494
BBa_K400628_sequence
1
atgggtaagtcacattcgccagttcactggaagtcggcggccgagatcgtcgagttagtcaagagcaaacaaatttccccacgcgaggtcgtggaaagcacgatcgacctcatcgagcaacgcgatcccggcctgaatgcggtcgtgtacaaggcttacgatgaagcgcgcgaaaaagcggctgcgcttgagcggcgcatcatgcaaggcgagcctgttggcatgctggcgggggttccgactctcatgaaggacttgttcgcggccaagccgggctggccgtccacgctgggcggcattcgcgccctcaaggacgcccgcggcgctgccggcgtctggtccacttatccattgaagatgtccggcgaagacagcctgttgctgggacagacaaacagccccgtctatggctttcgcggaaccacggacaacacgtttttcggtccgacgcgcaatccattcaacctcgacttcaacgcaggggggtcttcgggaggagccgctgcgctcgtggccgatggcatcgttcctgtggcaggcggcacggatggcggcggctcgatccgcattccggcggcatggaccaatacctacggctttcagccttcgataggacgcgttccgttcaaaagcccgccccaatgctttccatccggggaccgtatctctatgaaggagcccatcacgcggacggtgccggatgcggcattggcaatgaatgtactgcacggcttcgaccgccgcgatcctgcttcgctgcgagtcaagctggacttcacctcagcgcttgcacaaggcgtgcgtggcaaaaaaatcggcctgacgctgaattacggggtatttcccgtccagcaagagattcaggatttgatcggcaaggctgcccgggtttttacagagcttggcgcacacgtcgagttcgtcgacctcggtatcccctacagccagaaacaaatgagcgatgcctggtgccgcatgatcgcgattcctactgtcgcctcgatgcaggcgctgcgcaaggaaggcattgacttatatggcgaacatcgggcagacatacccgatgcgctgatgaagtggatagatgcggtcgctgacataagcgtccagcagatcagcgctgatcaactattgagaacgaccgttttcgattgcatgaacggcgtattcgatcgctttgacctattattggcgccgaccctggcatgcatgcctgttcgcaatgcgactgatggctgcaccgagggaccaagccagatcaacggtgaagaaatcgaccctttgatcggctggtgcatgacttacctgaccaatttttcagggcatcccagtgccagtgtcccggctggcctgattgatggcctgccggcaggcatgctcattatcggcgatcgccaggcggacctggatgtcattgccgcaagcgccgctttcgagcgagccagcccgtggtcccaatactatgacattcccgccggacggcccctgtgataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z