BBa_K400628 1 BBa_K400628 mAAA (EcoRI site mutated Aryl Acyl-Amidase) 2010-10-18T11:00:00Z 2015-05-08T01:12:23Z It was identified from a genomic library of a soil bacterium, which was isolated using a selection media having p-acetaminophenol as a sole carbon source. Aryl acylamidase is an enzyme that acts in the amide bond between aryl- and acyl- compounds. The most typical reaction is the hydrolysis of an anilide, producing a carboxylate and aniline, which is reversible. When a p-acetaminophenol was reacted with AAA, the product gave a purple-red color. As a result, a clone presenting AAA activity from the genomic library gave a color change around the colony when it was grown on solid media enriched with p-acetaminophenol. Since it gives color to colorless substance, it can be used as a reporter gene to a specific promoter. In our project, we put it downstream of cadmium-dependent promoter to detect any cadmium present in a solution. false false _511_ 0 6640 9 It's complicated false This part was cloned into pSB1C3 vector and mAAA was mutated in two EcoRI sites. false Seobeom Heo annotation2089461 1 mutated aryl acylamidase range2089461 1 1 1494 BBa_K400628_sequence 1 atgggtaagtcacattcgccagttcactggaagtcggcggccgagatcgtcgagttagtcaagagcaaacaaatttccccacgcgaggtcgtggaaagcacgatcgacctcatcgagcaacgcgatcccggcctgaatgcggtcgtgtacaaggcttacgatgaagcgcgcgaaaaagcggctgcgcttgagcggcgcatcatgcaaggcgagcctgttggcatgctggcgggggttccgactctcatgaaggacttgttcgcggccaagccgggctggccgtccacgctgggcggcattcgcgccctcaaggacgcccgcggcgctgccggcgtctggtccacttatccattgaagatgtccggcgaagacagcctgttgctgggacagacaaacagccccgtctatggctttcgcggaaccacggacaacacgtttttcggtccgacgcgcaatccattcaacctcgacttcaacgcaggggggtcttcgggaggagccgctgcgctcgtggccgatggcatcgttcctgtggcaggcggcacggatggcggcggctcgatccgcattccggcggcatggaccaatacctacggctttcagccttcgataggacgcgttccgttcaaaagcccgccccaatgctttccatccggggaccgtatctctatgaaggagcccatcacgcggacggtgccggatgcggcattggcaatgaatgtactgcacggcttcgaccgccgcgatcctgcttcgctgcgagtcaagctggacttcacctcagcgcttgcacaaggcgtgcgtggcaaaaaaatcggcctgacgctgaattacggggtatttcccgtccagcaagagattcaggatttgatcggcaaggctgcccgggtttttacagagcttggcgcacacgtcgagttcgtcgacctcggtatcccctacagccagaaacaaatgagcgatgcctggtgccgcatgatcgcgattcctactgtcgcctcgatgcaggcgctgcgcaaggaaggcattgacttatatggcgaacatcgggcagacatacccgatgcgctgatgaagtggatagatgcggtcgctgacataagcgtccagcagatcagcgctgatcaactattgagaacgaccgttttcgattgcatgaacggcgtattcgatcgctttgacctattattggcgccgaccctggcatgcatgcctgttcgcaatgcgactgatggctgcaccgagggaccaagccagatcaacggtgaagaaatcgaccctttgatcggctggtgcatgacttacctgaccaatttttcagggcatcccagtgccagtgtcccggctggcctgattgatggcctgccggcaggcatgctcattatcggcgatcgccaggcggacctggatgtcattgccgcaagcgccgctttcgagcgagccagcccgtggtcccaatactatgacattcccgccggacggcccctgtgataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z