LOCUS BBa_K404004 4402 bp DNA linear 26-Nov-2024 DEFINITION [AAV2]-Rep-VP13(ViralBrick-587KO-empty)_p5-TATAless ACCESSION BBa_K404004 VERSION BBa_K404004.1 FEATURES Location/Qualifiers CDS 1..1933 /label=Rep68 CDS 1..1867 /label=Rep78 promoter 410..529 /label=p19 CDS 673..1933 /label=Rep40 CDS 673..1867 /label=Rep52 promoter 1390..1507 /label=p40 CDS 1883..4216 /label=AAV-2-cap (Stratagene) CDS 1883..4090 /label=VP1 misc_feature 2015..2191 /label=PLA2 misc_feature 2240..2260 /label=basic region 1 misc_feature 2300..2320 /label=basic region 2 misc_feature 2378..2398 /label=basic region 3 CDS 2489..4090 /label=VP3 misc_feature 2801..2818 /label=basic region 4 promoter 4328..4401 /label=p5TATAless misc_feature 2296..2296 /label=G--> C VP2 KO misc_feature 3644..3646 /label=HSPK-KO misc_feature 3635..3637 /label=HSPG-KO misc_feature 4217..4327 /label=scar ORIGIN 1 atggcggggt tttacgagat tgtgattaag gtccccagcg accttgacga gcatctgccc 61 ggcatttctg acagctttgt gaactgggtg gccgagaagg aatgggagtt gccgccagat 121 tctgacatgg atctgaatct gattgagcag gcacccctga ccgtggccga gaagctccag 181 cgcgactttc tgacggaatg gcgccgtgtg agtaaggccc cggaggccct tttctttgtg 241 caatttgaga agggagagag ctacttccac atgcacgtgc tcgtggaaac caccggggtg 301 aaatccatgg ttttgggacg tttcctgagt cagattcgcg aaaaactgat tcagagaatt 361 taccgcggga tcgagccgac tttgccaaac tggttcgcgg tcacaaagac cagaaatggc 421 gccggaggcg ggaacaaggt ggtggatgag tgctacatcc ccaattactt gctccccaaa 481 acccagcctg agctccagtg ggcgtggact aatatggaac agtatttaag cgcctgtttg 541 aatctcacgg agcgtaaacg gttggtggcg cagcatctga cgcacgtgtc gcagacgcag 601 gagcagaaca aagagaatca gaatcccaat tctgatgcgc cggtgatcag atcaaaaact 661 tcagccaggt acatggagct ggtcgggtgg ctcgtggaca aggggattac ctcggagaag 721 cagtggattc aggaggacca ggcctcatac atctccttca atgcggcctc caactcgcgg 781 tcccaaatca aggctgcctt ggacaatgcg ggaaagatta tgagcctgac taaaaccgcc 841 cccgactacc tggtgggcca gcagcccgtg gaggacattt ccagcaatcg gatttataaa 901 attttggaac taaacgggta cgatccccaa tatgcggctt ccgtctttct gggatgggcc 961 acgaaaaagt tcggcaagag gaacaccatc tggctgtttg ggcctgcaac taccgggaag 1021 accaacatcg cggaggccat agcccacact gtgcccttct acgggtgcgt aaactggacc 1081 aatgagaact ttcccttcaa cgactgtgtg gacaagatgg tgatctggtg ggaggagggg 1141 aagatgaccg ccaaggtcgt ggagtcggcc aaagccattc tcggaggaag caaggtgcgc 1201 gtggaccaga aatgcaagtc ctcggcccag atagacccga ctcccgtgat cgtcacctcc 1261 aacaccaaca tgtgcgccgt gattgacggg aactcaacga ccttcgaaca ccagcagccg 1321 ttgcaagacc ggatgttcaa atttgaactc acccgccgtc tggatcatga ctttgggaag 1381 gtcaccaagc aggaagtcaa agactttttc cggtgggcaa aggatcacgt ggttgaggtg 1441 gagcatgagt tctacgtcaa aaagggtgga gccaagaaaa gacccgcccc cagtgacgca 1501 gatataagtg agcccaaacg ggtgcgcgag tcagttgcgc agccatcgac gtcagacgcg 1561 gaagcttcga tcaactacgc agacaggtac caaaacaaat gttctcgtca cgtgggcatg 1621 aatctgatgc tgtttccctg tagacaatgc gagagaatga atcagaactc aaatatctgc 1681 ttcactcacg gacagaaaga ctgtttagag tgctttcccg tgtcagaatc tcaacccgtt 1741 tctgtcgtca aaaaggcgta tcagaaactg tgctacattc atcatatcat gggaaaggtg 1801 ccagacgctt gcactgcctg cgatctggtc aatgtggatt tggatgactg catctttgaa 1861 caataaatga tttaaatcag gtatggctgc cgatggttat cttccagatt ggctcgagga 1921 cactctctct gaaggaataa gacagtggtg gaagctcaaa cctggcccac caccaccaaa 1981 gcccgcagag cggcataagg acgacagcag gggtcttgtg cttcctgggt acaagtacct 2041 cggacccttc aacggactcg acaagggaga gccggtcaac gaggcagacg ccgcggccct 2101 cgagcacgac aaagcctacg accggcagct cgacagcgga gacaacccgt acctcaagta 2161 caaccacgcc gacgcggagt ttcaggagcg ccttaaagaa gatacgtctt ttgggggcaa 2221 cctcggacga gcagtcttcc aggcgaaaaa gagggttctt gaacctctgg gcctggttga 2281 ggaacctgtt aagaccgctc cgggaaaaaa gaggccggta gagcactctc ctgtggagcc 2341 agactcctcc tcgggaaccg gaaaggcggg ccagcagcct gcaagaaaaa gattgaattt 2401 tggtcagact ggagacgcag actcagtacc tgacccccag cctctcggac agccaccagc 2461 agccccctct ggtctgggaa ctaatacgat ggctacaggc agtggcgcac caatggcaga 2521 caataacgag ggcgccgacg gagtgggtaa ttcctcggga aattggcatt gcgattccac 2581 atggatgggc gacagagtca tcaccaccag cacccgaacc tgggccctgc ccacctacaa 2641 caaccacctc tacaaacaaa tttccagcca atcaggagcc tcgaacgaca atcactactt 2701 tggctacagc accccttggg ggtattttga cttcaacaga ttccactgcc acttttcacc 2761 acgtgactgg caaagactca tcaacaacaa ctggggattc cgacccaaga gactcaactt 2821 caagctcttt aacattcaag tcaaagaggt cacgcagaat gacggtacga cgacgattgc 2881 caataacctt accagcacgg ttcaggtgtt tactgactcg gagtaccagc tcccgtacgt 2941 cctcggctcg gcgcatcaag gatgcctccc gccgttccca gcagacgtct tcatggtgcc 3001 acagtatgga tacctcaccc tgaacaacgg gagtcaggca gtaggacgct cttcatttta 3061 ctgcctggag tactttcctt ctcagatgct gcgtaccgga aacaacttta ccttcagcta 3121 cacttttgag gacgttcctt tccacagcag ctacgctcac agccagagtc tggaccgtct 3181 catgaatcct ctcatcgacc aatatttgta ttacttgagc agaacaaaca ctccaagtgg 3241 taccaccacg cagagtcgac ttcagttttc tcaggccgga gcgagtgaca ttcgggacca 3301 gtctaggaac tggcttcctg gaccctgtta ccgccagcag cgagtatcaa agacatctgc 3361 ggataacaac aacagtgaat actcgtggac tggagctacc aagtaccacc tcaatggcag 3421 agactctctg gtgaatccgg gcccggccat ggcaagccac aaggacgatg aagaaaagtt 3481 ttttcctcag agcggggttc tcatctttgg gaagcaaggc tcagagaaaa caaatgtgga 3541 cattgaaaag gtcatgatta cagacgaaga ggaaatcagg acaaccaatc ccgtggctac 3601 ggagcagtat ggatccgtat ctaccaacct ccaggctggc aacgcccaag cagctacagc 3661 tgatgtcaac acacaaggcg ttcttccagg catggtctgg caggacagag atgtgtacct 3721 tcaggggccc atctgggcaa agattccaca cacggacgga cattttcacc cctctcccct 3781 catgggtgga ttcggactta aacaccctcc tccacagatt ctcatcaaga acaccccggt 3841 acctgcgaat ccttcgacca ccttcagtgc ggcaaagttt gcttccttca tcacacagta 3901 ctccacggga caggtcagcg tggagatcga gtgggagctc cagaaggaaa acagcaaacg 3961 ctggaatccc gaaattcagt acacttccaa ctacaacaag tctgttaatg tggactttac 4021 tgtggacact aatggcgtgt attcagagcc tcgccccatt ggcaccagat acctgactcg 4081 taatctgtaa ttgcttgtta atcaataaac cgtttaattc gtttcagttg aactttggtc 4141 tctgcgtatt tctttcttat ctagtttcca tggctacgta gataagtagc atggcgggtt 4201 aatcattaac tacagcccgg gcgtttaaac agcgggcgga ggggtggagt cgtgacgtga 4261 attacgtcat agggttaggg aggtcctgta ttagaggtca cgtgagtgtt ttgcgacatt 4321 ttgcgacacc atgtggtctc gctggggggg ggggcccgag tgagcacgca gggtctccat 4381 tttgaagcgg gaggtttgaa ct //