LOCUS BBa_K404121 2826 bp DNA linear 03-Apr-2025 DEFINITION [AAV2]-left-ITR_pCMV_betaglobin_mCherry_hGH_[AAV2]-right-ITR ACCESSION BBa_K404121 VERSION BBa_K404121.1 FEATURES Location/Qualifiers misc_feature 1..147 /label=left ITR promoter 155..809 /label=CMV promoter misc_feature 816..1310 /label=Beta-Globin-intron misc_feature 1336..2046 /label=mCherry misc_feature 2069..2112 /label=rrnB_T1 misc_feature 2153..2172 /label=stem loop early T7 misc_feature 2688..2826 /label=right ITR misc_feature 2198..2664 /label=hGH ORIGIN 1 gtgcaggcag ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg 61 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa 121 ctccatcact aggggttcct gcggcctact agagcgatgt acgggccaga tatacgcgtt 181 gacattgatt attgcctagt tattaatagt aatcaattac ggggtcatta gttcatagcc 241 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 301 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 361 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 421 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 481 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 541 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 601 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 661 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 721 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag 781 aacccactgc ttactggctt atcgaaatta ctagagcgaa tcccggccgg gaacggtgca 841 ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga gtctataggc 901 ccacaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt ctaatacttt 961 ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct ttgcaccatt 1021 ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatttct gcatataaat 1081 atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata gcagctacaa 1141 tccagctacc attctgcttt tattttatgg ttgggataag gctggattat tctgagtcca 1201 agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca gctcctgggc 1261 aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag aattgggatt actagagaaa 1321 gaggagaaat actagatggt gagcaagggc gaggaggata acatggccat catcaaggag 1381 ttcatgcgct tcaaggtgca catggagggc tccgtgaacg gccacgagtt cgagatcgag 1441 ggcgagggcg agggccgccc ctacgagggc acccagaccg ccaagctgaa ggtgaccaag 1501 ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc agttcatgta cggctccaag 1561 gcctacgtga agcaccccgc cgacatcccc gactacttga agctgtcctt ccccgagggc 1621 ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac 1681 tcctccttgc aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc 1741 tccgacggcc ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg 1801 taccccgagg acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc 1861 ggccactacg acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc 1921 ggcgcctaca acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc 1981 gtggaacagt acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac 2041 aagtaataat actagagcca ggcatcaaat aaaacgaaag gctcagtcga aagactgggc 2101 ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca ctggctcacc 2161 ttcgggtggg cctttctgcg tttatatact agagacgggt ggcatccctg tgacccctcc 2221 ccagtgcctc tcctggccct ggaagttgcc actccagtgc ccaccagcct tgtcctaata 2281 aaattaagtt gcatcatttt gtctgactag gtgtccttct ataatattat ggggtggagg 2341 ggggtggtat ggagcaaggg gcaagttggg aagacaacct gtagggcctg cggggtctat 2401 tgggaaccaa gctggagtgc agtggcacaa tcttggctca ctgcaatctc cgcctcctgg 2461 gttcaagcga ttctcctgcc tcagcctccc gagttgttgg gattccaggc atgcatgacc 2521 aggctcagct aatttttgtt tttttggtag agacggggtt tcaccatatt ggccaggctg 2581 gtctccaact cctaatctca ggtgatctac ccaccttggc ctcccaaatt gctgggatta 2641 caggcgtgaa ccactgctcc cttccctgtc ctttactaga ggccgcagga acccctagtg 2701 atggagttgg ccactccctc tctgcgcgct cgctcgctca ctgaggccgg gcgaccaaag 2761 gtcgcccgac gcccgggctt tgcccgggcg gcctcagtga gcgagcgagc gcgcagctgc 2821 ctgcat //