LOCUS BBa_K404122 3659 bp DNA linear 25-Nov-2024 DEFINITION [AAV2]-left-ITR_pCMV_betaglobin_mGMK_TK30_hGH_[AAV2]-right-ITR ACCESSION BBa_K404122 VERSION BBa_K404122.1 FEATURES Location/Qualifiers misc_feature 6..140 /label=left ITR misc_feature 88..103 /label=RBE (Rep-binding element) promoter 155..808 /label=CMV misc_feature 817..1309 /label=Beta-Globin-intron promoter 824..904 /label=part of chimeric CMV promoter misc_feature 899..1252 /label=intron 2 of human beta-globin misc_feature 905..1251 /label=beta-globin-intron II misc_feature 1252..1304 /label=part of human beta-globin-exon III CDS 1316..3019 /label=mGMK_TK30 misc_feature 3028..3506 /label=hGH misc_feature 3046..3050 /label=DSE (downstream sequence element) misc_feature 3080..3081 /label=cleavage site polyA_site 3110..3115 /label=polyA misc_feature 3521..3658 /label=right ITR misc_feature 3632..3647 /label=RBE (Rep-binding element) misc_feature 141..154 /label=scar misc_feature 1..5 /label=scar misc_feature 809..816 /label=scar misc_feature 1310..1315 /label=scar misc_feature 3020..3027 /label=scar misc_feature 3507..3520 /label=scar ORIGIN 1 gtgcaggcag ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg 61 ggcgaccttt ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa 121 ctccatcact aggggttcct gcggcctact agagcgatgt acgggccaga tatacgcgtt 181 gacattgatt attgcctagt tattaatagt aatcaattac ggggtcatta gttcatagcc 241 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca 301 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga 361 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc 421 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct 481 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat 541 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc 601 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt 661 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa 721 tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctctctgg ctaactagag 781 aacccactgc ttactggctt atcgaaatta ctagagcgaa tcccggccgg gaacggtgca 841 ttggaacgcg gattccccgt gccaagagtg acgtaagtac cgcctataga gtctataggc 901 ccacaaaaaa tgctttcttc ttttaatata cttttttgtt tatcttattt ctaatacttt 961 ccctaatctc tttctttcag ggcaataatg atacaatgta tcatgcctct ttgcaccatt 1021 ctaaagaata acagtgataa tttctgggtt aaggcaatag caatatttct gcatataaat 1081 atttctgcat ataaattgta actgatgtaa gaggtttcat attgctaata gcagctacaa 1141 tccagctacc attctgcttt tattttatgg ttgggataag gctggattat tctgagtcca 1201 agctaggccc ttttgctaat catgttcata cctcttatct tcctcccaca gctcctgggc 1261 aacgtgctgg tctgtgtgct ggcccatcac tttggcaaag aattgggatt actagatggc 1321 cggcgcagga cctaggccag tagtgctgag cgggccgtca ggggcaggga agagcactct 1381 gctcaagaag ctgttccagg agcacagcag catcttcggc ttcagtgtgt cccatactac 1441 aaggaaccca cgacctggtg aagaagatgg caaagattac tactttgtga ccagggagat 1501 gatgcagcgt gatattgcag caggggactt cattgagcat gctgagttct cagggaacct 1561 gtacgggaca agcaaggaag ctgttcgggc tgtgcaggcc atgaaccgca tctgcgtgct 1621 agatgtcgac ctacaaggtg tgcgcagcat caagaagact gatctgtgtc ccatctacat 1681 ctttgtgcag cctccctcgc tggacgtgct ggagcaacga ctgcgactgc gcaacactga 1741 gactgaggag agtctggcaa agcggctggc agctgcacgg acagacatgg agagcagcaa 1801 ggagcctggc ttgtttgacc tggtgatcat caatgacgac ctggataaag cctatgcaac 1861 cctgaagcag gcgctctctg aggaaatcaa gaaagcacag ggaactggcg cgtctgcgtt 1921 cgaccaggct gcgcgttctc gcggccatag caaccgacgt acggcgttgc gccctcgccg 1981 gcagcaagaa gccacggaag tccgcccgga gcagaaaatg cccacgctac tgcgggttta 2041 tatagacggt ccccacggga tggggaaaac caccaccacg caactgctgg tggccctggg 2101 ttcgcgcgac gatatcgtct acgtacccga gccgatgact tactggcggg tgctgggggc 2161 ttccgagaca atcgcgaaca tctacaccac acaacaccgc ctcgaccagg gtgagatatc 2221 ggccggggac gcggcggtgg taatgacaag cgcccagata acaatgggca tgccttatgc 2281 cgtgaccgac gccgttctgg ctcctcatat cgggggggag gctgggagct cacatgtccc 2341 gcccccggcc ctcaccattt tggctgaccg ccatcccatc gcatatttct tatgctaccc 2401 ggccgcgcgg taccttatgg gcagcatgac cccccaggcc gtgctggcgt tcgtggccct 2461 catcccgccg accttgcccg gcaccaacat cgtgcttggg gcccttccgg aggacagaca 2521 catcgaccgc ctggccaaac gccagcgccc cggcgagcgg ctggacctgg ctatgctggc 2581 tgcgattcgc cgcgtttacg ggctacttgc caatacggtg cggtatctgc aatgcggcgg 2641 gtcgtggcgg gaggactggg gacagctttc ggggacggcc gtgccgcccc agggtgccga 2701 gccccagagc aacgcgggcc cacgacccca tatcggggac acgttattta ccctgtttcg 2761 ggcccccgag ttgctggccc ccaacggcga cctgtataac gtgtttgcct gggccttgga 2821 cgtcttggcc aaacgcctcc gttccatgca cgtctttatc ctggattacg accaatcgcc 2881 cgccggctgc cgggacgccc tgctgcaact tacctccggg atggtccaga cccacgtcac 2941 cacccccggc tccataccga cgatatgcga cctggcgcgc acgtttgccc gggagatggg 3001 ggaggctaac accggttaat actagagacg ggtggcatcc ctgtgacccc tccccagtgc 3061 ctctcctggc cctggaagtt gccactccag tgcccaccag ccttgtccta ataaaattaa 3121 gttgcatcat tttgtctgac taggtgtcct tctataatat tatggggtgg aggggggtgg 3181 tatggagcaa ggggcaagtt gggaagacaa cctgtagggc ctgcggggtc tattgggaac 3241 caagctggag tgcagtggca caatcttggc tcactgcaat ctccgcctcc tgggttcaag 3301 cgattctcct gcctcagcct cccgagttgt tgggattcca ggcatgcatg accaggctca 3361 gctaattttt gtttttttgg tagagacggg gtttcaccat attggccagg ctggtctcca 3421 actcctaatc tcaggtgatc tacccacctt ggcctcccaa attgctggga ttacaggcgt 3481 gaaccactgc tcccttccct gtcctttact agaggccgca ggaaccccta gtgatggagt 3541 tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca aaggtcgccc 3601 gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc tgcctgcat //