LOCUS BBa_K404155 2785 bp DNA linear 27-Dec-2024 DEFINITION pCMV_Z-EGFR-1907_Short-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty) ACCESSION BBa_K404155 VERSION BBa_K404155.1 FEATURES Location/Qualifiers promoter 2..655 /label=CMV misc_feature 641..844 /label=ZEGFR_1907 misc_feature 851..862 /label=Short-linker misc_feature 869..2662 /label=VP2 misc_feature 1061..2662 /label=VP3 misc_feature 869..2785 /label=AAV-2-cap (Stratagene) misc_feature 2216..2218 /label=HSPG-KO misc_feature 2207..2209 /label=HSPG-KO ORIGIN 1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat 61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta 661 gatggccggc gtggataaca aatttaacaa agaaatgtgg gcggcgtggg aagaaattcg 721 taacctgccg aacctgaacg gctggcagat gaccgcgttt attgcgagcc tggtggatga 781 tccgagccag agcgcgaacc tgctggcgga agcgaaaaaa ctgaacgatg cgcaggcgcc 841 gaaaaccggc ggtggttctg gtaccggcgc tccgggaaaa aagaggccgg tagagcactc 901 tcctgtggag ccagactcct cctcgggaac cggaaaggcg ggccagcagc ctgcaagaaa 961 aagattgaat tttggtcaga ctggagacgc agactcagta cctgaccccc agcctctcgg 1021 acagccacca gcagccccct ctggtctggg aactaatacg atggctacag gcagtggcgc 1081 accaatggca gacaataacg agggcgccga cggagtgggt aattcctcgg gaaattggca 1141 ttgcgattcc acatggatgg gcgacagagt catcaccacc agcacccgaa cctgggccct 1201 gcccacctac aacaaccacc tctacaaaca aatttccagc caatcaggag cctcgaacga 1261 caatcactac tttggctaca gcaccccttg ggggtatttt gacttcaaca gattccactg 1321 ccacttttca ccacgtgact ggcaaagact catcaacaac aactggggat tccgacccaa 1381 gagactcaac ttcaagctct ttaacattca agtcaaagag gtcacgcaga atgacggtac 1441 gacgacgatt gccaataacc ttaccagcac ggttcaggtg tttactgact cggagtacca 1501 gctcccgtac gtcctcggct cggcgcatca aggatgcctc ccgccgttcc cagcagacgt 1561 cttcatggtg ccacagtatg gatacctcac cctgaacaac gggagtcagg cagtaggacg 1621 ctcttcattt tactgcctgg agtactttcc ttctcagatg ctgcgtaccg gaaacaactt 1681 taccttcagc tacacttttg aggacgttcc tttccacagc agctacgctc acagccagag 1741 tctggaccgt ctcatgaatc ctctcatcga ccaatatttg tattacttga gcagaacaaa 1801 cactccaagt ggtaccacca cgcagagtcg acttcagttt tctcaggccg gagcgagtga 1861 cattcgggac cagtctagga actggcttcc tggaccctgt taccgccagc agcgagtatc 1921 aaagacatct gcggataaca acaacagtga atactcgtgg actggagcta ccaagtacca 1981 cctcaatggc agagactctc tggtgaatcc gggcccggcc atggcaagcc acaaggacga 2041 tgaagaaaag ttttttcctc agagcggggt tctcatcttt gggaagcaag gctcagagaa 2101 aacaaatgtg gacattgaaa aggtcatgat tacagacgaa gaggaaatca ggacaaccaa 2161 tcccgtggct acggagcagt atggatccgt atctaccaac ctccaggctg gcaacgccca 2221 agcagctaca gctgatgtca acacacaagg cgttcttcca ggcatggtct ggcaggacag 2281 agatgtgtac cttcaggggc ccatctgggc aaagattcca cacacggacg gacattttca 2341 cccctctccc ctcatgggtg gattcggact taaacaccct cctccacaga ttctcatcaa 2401 gaacaccccg gtacctgcga atccttcgac caccttcagt gcggcaaagt ttgcttcctt 2461 catcacacag tactccacgg gacaggtcag cgtggagatc gagtgggagc tccagaagga 2521 aaacagcaaa cgctggaatc ccgaaattca gtacacttcc aactacaaca agtctgttaa 2581 tgtggacttt actgtggaca ctaatggcgt gtattcagag cctcgcccca ttggcaccag 2641 atacctgact cgtaatctgt aattgcttgt taatcaataa accgtttaat tcgtttcagt 2701 tgaactttgg tctctgcgta tttctttctt atctagtttc catggctacg tagataagta 2761 gcatggcggg ttaatcatta actac //