LOCUS BBa_K404157 2809 bp DNA linear 22-Dec-2024 DEFINITION pCMV_Z-EGFR-1907_Long-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty) ACCESSION BBa_K404157 VERSION BBa_K404157.1 FEATURES Location/Qualifiers promoter 2..655 /label=CMV misc_feature 671..844 /label=ZEGFR_1907 misc_feature 851..886 /label=long-linker misc_feature 893..2686 /label=VP2 misc_feature 1085..2686 /label=VP3 misc_feature 893..2809 /label=AAV-2-cap (Stratagene) misc_feature 2240..2242 /label=HSPG-KO misc_feature 2231..2233 /label=HSPG-KO ORIGIN 1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat 61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta 661 gatggccggc gtggataaca aatttaacaa agaaatgtgg gcggcgtggg aagaaattcg 721 taacctgccg aacctgaacg gctggcagat gaccgcgttt attgcgagcc tggtggatga 781 tccgagccag agcgcgaacc tgctggcgga agcgaaaaaa ctgaacgatg cgcaggcgcc 841 gaaaaccggc ggtggttctg gtggtggttc tggtggtggt tctggtaccg gcgctccggg 901 aaaaaagagg ccggtagagc actctcctgt ggagccagac tcctcctcgg gaaccggaaa 961 ggcgggccag cagcctgcaa gaaaaagatt gaattttggt cagactggag acgcagactc 1021 agtacctgac ccccagcctc tcggacagcc accagcagcc ccctctggtc tgggaactaa 1081 tacgatggct acaggcagtg gcgcaccaat ggcagacaat aacgagggcg ccgacggagt 1141 gggtaattcc tcgggaaatt ggcattgcga ttccacatgg atgggcgaca gagtcatcac 1201 caccagcacc cgaacctggg ccctgcccac ctacaacaac cacctctaca aacaaatttc 1261 cagccaatca ggagcctcga acgacaatca ctactttggc tacagcaccc cttgggggta 1321 ttttgacttc aacagattcc actgccactt ttcaccacgt gactggcaaa gactcatcaa 1381 caacaactgg ggattccgac ccaagagact caacttcaag ctctttaaca ttcaagtcaa 1441 agaggtcacg cagaatgacg gtacgacgac gattgccaat aaccttacca gcacggttca 1501 ggtgtttact gactcggagt accagctccc gtacgtcctc ggctcggcgc atcaaggatg 1561 cctcccgccg ttcccagcag acgtcttcat ggtgccacag tatggatacc tcaccctgaa 1621 caacgggagt caggcagtag gacgctcttc attttactgc ctggagtact ttccttctca 1681 gatgctgcgt accggaaaca actttacctt cagctacact tttgaggacg ttcctttcca 1741 cagcagctac gctcacagcc agagtctgga ccgtctcatg aatcctctca tcgaccaata 1801 tttgtattac ttgagcagaa caaacactcc aagtggtacc accacgcaga gtcgacttca 1861 gttttctcag gccggagcga gtgacattcg ggaccagtct aggaactggc ttcctggacc 1921 ctgttaccgc cagcagcgag tatcaaagac atctgcggat aacaacaaca gtgaatactc 1981 gtggactgga gctaccaagt accacctcaa tggcagagac tctctggtga atccgggccc 2041 ggccatggca agccacaagg acgatgaaga aaagtttttt cctcagagcg gggttctcat 2101 ctttgggaag caaggctcag agaaaacaaa tgtggacatt gaaaaggtca tgattacaga 2161 cgaagaggaa atcaggacaa ccaatcccgt ggctacggag cagtatggat ccgtatctac 2221 caacctccag gctggcaacg cccaagcagc tacagctgat gtcaacacac aaggcgttct 2281 tccaggcatg gtctggcagg acagagatgt gtaccttcag gggcccatct gggcaaagat 2341 tccacacacg gacggacatt ttcacccctc tcccctcatg ggtggattcg gacttaaaca 2401 ccctcctcca cagattctca tcaagaacac cccggtacct gcgaatcctt cgaccacctt 2461 cagtgcggca aagtttgctt ccttcatcac acagtactcc acgggacagg tcagcgtgga 2521 gatcgagtgg gagctccaga aggaaaacag caaacgctgg aatcccgaaa ttcagtacac 2581 ttccaactac aacaagtctg ttaatgtgga ctttactgtg gacactaatg gcgtgtattc 2641 agagcctcgc cccattggca ccagatacct gactcgtaat ctgtaattgc ttgttaatca 2701 ataaaccgtt taattcgttt cagttgaact ttggtctctg cgtatttctt tcttatctag 2761 tttccatggc tacgtagata agtagcatgg cgggttaatc attaactac //