LOCUS BBa_K404158 2881 bp DNA linear 15-Nov-2024 DEFINITION pCMV_Z-EGFR-1907_SEG-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty) ACCESSION BBa_K404158 VERSION BBa_K404158.1 FEATURES Location/Qualifiers promoter 2..655 /label=CMV misc_feature 671..844 /label=ZEGFR_1907 misc_feature 851..858 /label=SEG-linker misc_feature 965..2758 /label=VP2 misc_feature 1157..2758 /label=VP3 misc_feature 965..2881 /label=AAV-2-cap (Stratagene) misc_feature 2312..2314 /label=HSPG-KO misc_feature 2303..2305 /label=HSPG-KO ORIGIN 1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat 61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta 661 gatggccggc gtggataaca aatttaacaa agaaatgtgg gcggcgtggg aagaaattcg 721 taacctgccg aacctgaacg gctggcagat gaccgcgttt attgcgagcc tggtggatga 781 tccgagccag agcgcgaacc tgctggcgga agcgaaaaaa ctgaacgatg cgcaggcgcc 841 gaaaaccggc ggtggttctg gcggcggttc tgaaggtggc ggctccgaag gcggcggcag 901 cgagggcggt ggtagcgaag gtggtggctc cgagggtggc ggttccggcg gcggtagcac 961 cggcgctccg ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc 1021 gggaaccgga aaggcgggcc agcagcctgc aagaaaaaga ttgaattttg gtcagactgg 1081 agacgcagac tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg 1141 tctgggaact aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg 1201 cgccgacgga gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga 1261 cagagtcatc accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta 1321 caaacaaatt tccagccaat caggagcctc gaacgacaat cactactttg gctacagcac 1381 cccttggggg tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca 1441 aagactcatc aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa 1501 cattcaagtc aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac 1561 cagcacggtt caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc 1621 gcatcaagga tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata 1681 cctcaccctg aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta 1741 ctttccttct cagatgctgc gtaccggaaa caactttacc ttcagctaca cttttgagga 1801 cgttcctttc cacagcagct acgctcacag ccagagtctg gaccgtctca tgaatcctct 1861 catcgaccaa tatttgtatt acttgagcag aacaaacact ccaagtggta ccaccacgca 1921 gagtcgactt cagttttctc aggccggagc gagtgacatt cgggaccagt ctaggaactg 1981 gcttcctgga ccctgttacc gccagcagcg agtatcaaag acatctgcgg ataacaacaa 2041 cagtgaatac tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt 2101 gaatccgggc ccggccatgg caagccacaa ggacgatgaa gaaaagtttt ttcctcagag 2161 cggggttctc atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt 2221 catgattaca gacgaagagg aaatcaggac aaccaatccc gtggctacgg agcagtatgg 2281 atccgtatct accaacctcc aggctggcaa cgcccaagca gctacagctg atgtcaacac 2341 acaaggcgtt cttccaggca tggtctggca ggacagagat gtgtaccttc aggggcccat 2401 ctgggcaaag attccacaca cggacggaca ttttcacccc tctcccctca tgggtggatt 2461 cggacttaaa caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc 2521 ttcgaccacc ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacgggaca 2581 ggtcagcgtg gagatcgagt gggagctcca gaaggaaaac agcaaacgct ggaatcccga 2641 aattcagtac acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa 2701 tggcgtgtat tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaatt 2761 gcttgttaat caataaaccg tttaattcgt ttcagttgaa ctttggtctc tgcgtatttc 2821 tttcttatct agtttccatg gctacgtaga taagtagcat ggcgggttaa tcattaacta 2881 c //