LOCUS       BBa_K404159             3337 bp    DNA     linear       14-Jun-2024
DEFINITION  pCMV_CFP_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
ACCESSION   BBa_K404159
VERSION     BBa_K404159.1  
FEATURES             Location/Qualifiers
     promoter        2..655
                     /label=CMV
     misc_feature    662..1385
                     /label=CFP
     misc_feature    1391..1414
                     /label=middle-linker
     misc_feature    1421..3214
                     /label=VP2
     misc_feature    1613..3214
                     /label=VP3
     misc_feature    1421..3337
                     /label=AAV-2-cap (Stratagene)
     misc_feature    2768..2770
                     /label=HSPG-KO
     misc_feature    2759..2761
                     /label=HSPG-KO
ORIGIN
        1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat
       61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta
      661 gatggccggc atgagcaaag gcgaagaact gtttaccggc gtggtgccga ttctggtgga
      721 actggatggt gatgtgaacg gccataaatt tagcgtgagc ggcgaaggcg aaggtgatgc
      781 gacctatggc aaactgaccc tgaaatttat ttgcaccacg ggtaaactgc cggttccgtg
      841 gccgaccctg gttaccaccc tgacctgggg cgtgcagtgc tttgcgcgtt atccggatca
      901 tatgaaacag cacgatttct ttaaaagcgc catgccggaa ggctatgtgc aggaacgcac
      961 catctttttt aaagatgatg gcaactataa aacccgtgcg gaagtgaaat ttgaaggcga
     1021 taccctggtg aaccgtattg aactgaaagg catcgatttc aaagaagatg gcaacattct
     1081 gggccataaa ctggaatata acgcgattag cgataacgtg tatatcaccg cggataaaca
     1141 gaaaaacggc atcaaagcga actttaaaat ccgccacaac attgaagatg gcagcgtgca
     1201 gctggccgat cattatcagc agaacacccc gattggtgat ggcccggtgc tgctgccgga
     1261 taaccattat ctgagcaccc agagcaaact gagcaaagat ccgaacgaaa aacgtgacca
     1321 tatggtgctg ctggaatttg tgaccgcggc cggtattacc catggcatgg atgaactgta
     1381 taaaaccggc ggtggttctg gtggtggttc tggtaccggc gctccgggaa aaaagaggcc
     1441 ggtagagcac tctcctgtgg agccagactc ctcctcggga accggaaagg cgggccagca
     1501 gcctgcaaga aaaagattga attttggtca gactggagac gcagactcag tacctgaccc
     1561 ccagcctctc ggacagccac cagcagcccc ctctggtctg ggaactaata cgatggctac
     1621 aggcagtggc gcaccaatgg cagacaataa cgagggcgcc gacggagtgg gtaattcctc
     1681 gggaaattgg cattgcgatt ccacatggat gggcgacaga gtcatcacca ccagcacccg
     1741 aacctgggcc ctgcccacct acaacaacca cctctacaaa caaatttcca gccaatcagg
     1801 agcctcgaac gacaatcact actttggcta cagcacccct tgggggtatt ttgacttcaa
     1861 cagattccac tgccactttt caccacgtga ctggcaaaga ctcatcaaca acaactgggg
     1921 attccgaccc aagagactca acttcaagct ctttaacatt caagtcaaag aggtcacgca
     1981 gaatgacggt acgacgacga ttgccaataa ccttaccagc acggttcagg tgtttactga
     2041 ctcggagtac cagctcccgt acgtcctcgg ctcggcgcat caaggatgcc tcccgccgtt
     2101 cccagcagac gtcttcatgg tgccacagta tggatacctc accctgaaca acgggagtca
     2161 ggcagtagga cgctcttcat tttactgcct ggagtacttt ccttctcaga tgctgcgtac
     2221 cggaaacaac tttaccttca gctacacttt tgaggacgtt cctttccaca gcagctacgc
     2281 tcacagccag agtctggacc gtctcatgaa tcctctcatc gaccagtacc tgtattactt
     2341 gagcagaaca aacactccaa gtggaaccac cacgcagtca aggcttcagt tttctcaggc
     2401 cggagcgagt gacattcggg accagtctag gaactggctt cctggaccct gttaccgcca
     2461 gcagcgagta tcaaagacat ctgcggataa caacaacagt gaatactcgt ggactggagc
     2521 taccaagtac cacctcaatg gcagagactc tctggtgaat ccgggcccgg ccatggcaag
     2581 ccacaaggac gatgaagaaa agttttttcc tcagagcggg gttctcatct ttgggaagca
     2641 aggctcagag aaaacaaatg tggacattga aaaggtcatg attacagacg aagaggaaat
     2701 caggacaacc aatcccgtgg ctacggagca gtatggttct gtatctacca acctccaggc
     2761 tggcaacgcc caagcagcta ccgcagatgt caacacacaa ggcgttcttc caggcatggt
     2821 ctggcaggac agagatgtgt accttcaggg gcccatctgg gcaaagattc cacacacgga
     2881 cggacatttt cacccctctc ccctcatggg tggattcgga cttaaacacc ctcctccaca
     2941 gattctcatc aagaacaccc cggtacctgc gaatccttcg accaccttca gtgcggcaaa
     3001 gtttgcttcc ttcatcacac agtactccac gggacaggtc agcgtggaga tcgagtggga
     3061 gctccagaag gaaaacagca aacgctggaa tcccgaaatt cagtacactt ccaactacaa
     3121 caagtctgtt aatgtggact ttactgtgga cactaatggc gtgtattcag agcctcgccc
     3181 cattggcacc agatacctga ctcgtaatct gtaattgctt gttaatcaat aaaccgttta
     3241 attcgtttca gttgaacttt ggtctctgcg tatttctttc ttatctagtt tccatggcta
     3301 cgtagataag tagcatggcg ggttaatcat taactac
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