LOCUS BBa_K404160 2641 bp DNA linear 22-Nov-2024 DEFINITION pCMV_His-Tag_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty) ACCESSION BBa_K404160 VERSION BBa_K404160.1 FEATURES Location/Qualifiers promoter 2..655 /label=CMV misc_feature 671..688 /label=His-tag misc_feature 695..718 /label=middle.linker misc_feature 725..2518 /label=VP2 misc_feature 917..2518 /label=VP3 misc_feature 725..2641 /label=AAV-2-cap (Stratagene) misc_feature 2072..2074 /label=HSPG-KO misc_feature 2063..2065 /label=HSPG-KO ORIGIN 1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat 61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta 661 gatggccggc catcatcacc atcaccacac cggcggtggt tctggtggtg gttctggtac 721 cggcgctccg ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc 781 gggaaccgga aaggcgggcc agcagcctgc aagaaaaaga ttgaattttg gtcagactgg 841 agacgcagac tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg 901 tctgggaact aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg 961 cgccgacgga gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga 1021 cagagtcatc accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta 1081 caaacaaatt tccagccaat caggagcctc gaacgacaat cactactttg gctacagcac 1141 cccttggggg tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca 1201 aagactcatc aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa 1261 cattcaagtc aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac 1321 cagcacggtt caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc 1381 gcatcaagga tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata 1441 cctcaccctg aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta 1501 ctttccttct cagatgctgc gtaccggaaa caactttacc ttcagctaca cttttgagga 1561 cgttcctttc cacagcagct acgctcacag ccagagtctg gaccgtctca tgaatcctct 1621 catcgaccag tacctgtatt acttgagcag aacaaacact ccaagtggaa ccaccacgca 1681 gtcaaggctt cagttttctc aggccggagc gagtgacatt cgggaccagt ctaggaactg 1741 gcttcctgga ccctgttacc gccagcagcg agtatcaaag acatctgcgg ataacaacaa 1801 cagtgaatac tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt 1861 gaatccgggc ccggccatgg caagccacaa ggacgatgaa gaaaagtttt ttcctcagag 1921 cggggttctc atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt 1981 catgattaca gacgaagagg aaatcaggac aaccaatccc gtggctacgg agcagtatgg 2041 ttctgtatct accaacctcc aggctggcaa cgcccaagca gctaccgcag atgtcaacac 2101 acaaggcgtt cttccaggca tggtctggca ggacagagat gtgtaccttc aggggcccat 2161 ctgggcaaag attccacaca cggacggaca ttttcacccc tctcccctca tgggtggatt 2221 cggacttaaa caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc 2281 ttcgaccacc ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacgggaca 2341 ggtcagcgtg gagatcgagt gggagctcca gaaggaaaac agcaaacgct ggaatcccga 2401 aattcagtac acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa 2461 tggcgtgtat tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaatt 2521 gcttgttaat caataaaccg tttaattcgt ttcagttgaa ctttggtctc tgcgtatttc 2581 tttcttatct agtttccatg gctacgtaga taagtagcat ggcgggttaa tcattaacta 2641 c //