LOCUS       BBa_K404160             2641 bp    DNA     linear       15-Jun-2024
DEFINITION  pCMV_His-Tag_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
ACCESSION   BBa_K404160
VERSION     BBa_K404160.1  
FEATURES             Location/Qualifiers
     promoter        2..655
                     /label=CMV
     misc_feature    671..688
                     /label=His-tag
     misc_feature    695..718
                     /label=middle.linker
     misc_feature    725..2518
                     /label=VP2
     misc_feature    917..2518
                     /label=VP3
     misc_feature    725..2641
                     /label=AAV-2-cap (Stratagene)
     misc_feature    2072..2074
                     /label=HSPG-KO
     misc_feature    2063..2065
                     /label=HSPG-KO
ORIGIN
        1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat
       61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta
      661 gatggccggc catcatcacc atcaccacac cggcggtggt tctggtggtg gttctggtac
      721 cggcgctccg ggaaaaaaga ggccggtaga gcactctcct gtggagccag actcctcctc
      781 gggaaccgga aaggcgggcc agcagcctgc aagaaaaaga ttgaattttg gtcagactgg
      841 agacgcagac tcagtacctg acccccagcc tctcggacag ccaccagcag ccccctctgg
      901 tctgggaact aatacgatgg ctacaggcag tggcgcacca atggcagaca ataacgaggg
      961 cgccgacgga gtgggtaatt cctcgggaaa ttggcattgc gattccacat ggatgggcga
     1021 cagagtcatc accaccagca cccgaacctg ggccctgccc acctacaaca accacctcta
     1081 caaacaaatt tccagccaat caggagcctc gaacgacaat cactactttg gctacagcac
     1141 cccttggggg tattttgact tcaacagatt ccactgccac ttttcaccac gtgactggca
     1201 aagactcatc aacaacaact ggggattccg acccaagaga ctcaacttca agctctttaa
     1261 cattcaagtc aaagaggtca cgcagaatga cggtacgacg acgattgcca ataaccttac
     1321 cagcacggtt caggtgttta ctgactcgga gtaccagctc ccgtacgtcc tcggctcggc
     1381 gcatcaagga tgcctcccgc cgttcccagc agacgtcttc atggtgccac agtatggata
     1441 cctcaccctg aacaacggga gtcaggcagt aggacgctct tcattttact gcctggagta
     1501 ctttccttct cagatgctgc gtaccggaaa caactttacc ttcagctaca cttttgagga
     1561 cgttcctttc cacagcagct acgctcacag ccagagtctg gaccgtctca tgaatcctct
     1621 catcgaccag tacctgtatt acttgagcag aacaaacact ccaagtggaa ccaccacgca
     1681 gtcaaggctt cagttttctc aggccggagc gagtgacatt cgggaccagt ctaggaactg
     1741 gcttcctgga ccctgttacc gccagcagcg agtatcaaag acatctgcgg ataacaacaa
     1801 cagtgaatac tcgtggactg gagctaccaa gtaccacctc aatggcagag actctctggt
     1861 gaatccgggc ccggccatgg caagccacaa ggacgatgaa gaaaagtttt ttcctcagag
     1921 cggggttctc atctttggga agcaaggctc agagaaaaca aatgtggaca ttgaaaaggt
     1981 catgattaca gacgaagagg aaatcaggac aaccaatccc gtggctacgg agcagtatgg
     2041 ttctgtatct accaacctcc aggctggcaa cgcccaagca gctaccgcag atgtcaacac
     2101 acaaggcgtt cttccaggca tggtctggca ggacagagat gtgtaccttc aggggcccat
     2161 ctgggcaaag attccacaca cggacggaca ttttcacccc tctcccctca tgggtggatt
     2221 cggacttaaa caccctcctc cacagattct catcaagaac accccggtac ctgcgaatcc
     2281 ttcgaccacc ttcagtgcgg caaagtttgc ttccttcatc acacagtact ccacgggaca
     2341 ggtcagcgtg gagatcgagt gggagctcca gaaggaaaac agcaaacgct ggaatcccga
     2401 aattcagtac acttccaact acaacaagtc tgttaatgtg gactttactg tggacactaa
     2461 tggcgtgtat tcagagcctc gccccattgg caccagatac ctgactcgta atctgtaatt
     2521 gcttgttaat caataaaccg tttaattcgt ttcagttgaa ctttggtctc tgcgtatttc
     2581 tttcttatct agtttccatg gctacgtaga taagtagcat ggcgggttaa tcattaacta
     2641 c
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