LOCUS       BBa_K404161             3082 bp    DNA     linear       22-Nov-2024
DEFINITION  pCMV_DARPin-E01_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty)
ACCESSION   BBa_K404161
VERSION     BBa_K404161.1  
FEATURES             Location/Qualifiers
     promoter        2..655
                     /label=CMV 
     misc_feature    666..1130
                     /label=DARPin-E01
     misc_feature    1136..1159
                     /label=middle-linker
     misc_feature    1166..2959
                     /label=VP2
     misc_feature    1358..2959
                     /label=VP3
     misc_feature    1166..3082
                     /label=AAV-2-cap (Stratagene)
     misc_feature    2513..2515
                     /label=HSPG-KO
     misc_feature    2504..2506
                     /label=HSPG-KO
ORIGIN
        1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat
       61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta
      661 gatggccggc gacctgggca agaagctgct ggaagccgcc agagccggac aggacgacga
      721 agtgcggatc ctgatggcca acggcgccga cgtgaacgcc gacgatacct ggggctggac
      781 ccctctgcac ctggccgcct atcagggcca cctggaaatc gtggaagtgc tgctgaagaa
      841 cggggccgat gtgaatgcct acgactacat cggctggaca cccctgcatc tcgctgccga
      901 cggccacctc gagattgtcg aggtcctgct gaaaaatggc gctgatgtga acgccagcga
      961 ttatatcggc gacacccccc tccatctggc cgcccacaac ggccatctgg aaattgtcga
     1021 agtcctcctc aagcacggcg cagatgtcaa cgcccaggac aagttcggca agaccgcctt
     1081 cgacatcagc atcgacaacg gcaacgagga cctggccgag atcctccaga ccggcggtgg
     1141 ttctggtggt ggttctggta ccggcgctcc gggaaaaaag aggccggtag agcactctcc
     1201 tgtggagcca gactcctcct cgggaaccgg aaaggcgggc cagcagcctg caagaaaaag
     1261 attgaatttt ggtcagactg gagacgcaga ctcagtacct gacccccagc ctctcggaca
     1321 gccaccagca gccccctctg gtctgggaac taatacgatg gctacaggca gtggcgcacc
     1381 aatggcagac aataacgagg gcgccgacgg agtgggtaat tcctcgggaa attggcattg
     1441 cgattccaca tggatgggcg acagagtcat caccaccagc acccgaacct gggccctgcc
     1501 cacctacaac aaccacctct acaaacaaat ttccagccaa tcaggagcct cgaacgacaa
     1561 tcactacttt ggctacagca ccccttgggg gtattttgac ttcaacagat tccactgcca
     1621 cttttcacca cgtgactggc aaagactcat caacaacaac tggggattcc gacccaagag
     1681 actcaacttc aagctcttta acattcaagt caaagaggtc acgcagaatg acggtacgac
     1741 gacgattgcc aataacctta ccagcacggt tcaggtgttt actgactcgg agtaccagct
     1801 cccgtacgtc ctcggctcgg cgcatcaagg atgcctcccg ccgttcccag cagacgtctt
     1861 catggtgcca cagtatggat acctcaccct gaacaacggg agtcaggcag taggacgctc
     1921 ttcattttac tgcctggagt actttccttc tcagatgctg cgtaccggaa acaactttac
     1981 cttcagctac acttttgagg acgttccttt ccacagcagc tacgctcaca gccagagtct
     2041 ggaccgtctc atgaatcctc tcatcgacca atatttgtat tacttgagca gaacaaacac
     2101 tccaagtggt accaccacgc agagtcgact tcagttttct caggccggag cgagtgacat
     2161 tcgggaccag tctaggaact ggcttcctgg accctgttac cgccagcagc gagtatcaaa
     2221 gacatctgcg gataacaaca acagtgaata ctcgtggact ggagctacca agtaccacct
     2281 caatggcaga gactctctgg tgaatccggg cccggccatg gcaagccaca aggacgatga
     2341 agaaaagttt tttcctcaga gcggggttct catctttggg aagcaaggct cagagaaaac
     2401 aaatgtggac attgaaaagg tcatgattac agacgaagag gaaatcagga caaccaatcc
     2461 cgtggctacg gagcagtatg gatccgtatc taccaacctc caggctggca acgcccaagc
     2521 agctacagct gatgtcaaca cacaaggcgt tcttccaggc atggtctggc aggacagaga
     2581 tgtgtacctt caggggccca tctgggcaaa gattccacac acggacggac attttcaccc
     2641 ctctcccctc atgggtggat tcggacttaa acaccctcct ccacagattc tcatcaagaa
     2701 caccccggta cctgcgaatc cttcgaccac cttcagtgcg gcaaagtttg cttccttcat
     2761 cacacagtac tccacgggac aggtcagcgt ggagatcgag tgggagctcc agaaggaaaa
     2821 cagcaaacgc tggaatcccg aaattcagta cacttccaac tacaacaagt ctgttaatgt
     2881 ggactttact gtggacacta atggcgtgta ttcagagcct cgccccattg gcaccagata
     2941 cctgactcgt aatctgtaat tgcttgttaa tcaataaacc gtttaattcg tttcagttga
     3001 actttggtct ctgcgtattt ctttcttatc tagtttccat ggctacgtag ataagtagca
     3061 tggcgggtta atcattaact ac
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