LOCUS BBa_K404162 2857 bp DNA linear 29-Nov-2024 DEFINITION pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-BAP) ACCESSION BBa_K404162 VERSION BBa_K404162.1 FEATURES Location/Qualifiers promoter 2..655 /label=CMV misc_feature 671..844 /label=ZEGFR_1907 misc_feature 851..874 /label=middle-linker misc_feature 881..2734 /label=VP2 misc_feature 1073..2734 /label=VP3 misc_feature 881..2857 /label=AAV-2-cap (Stratagene) misc_feature 2234..2278 /label=BAP misc_feature 2228..2233 /label=TG-spacer misc_feature 2279..2287 /label=GLS-spacer misc_feature 2288..2303 /label=downstream 587 misc_feature 2196..2227 /label=upstream 587 misc_feature 2219..2221 /label=HSPG-KO misc_feature 2288..2290 /label=HSPG-KO ORIGIN 1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat 61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta 661 gatggccggc gtggataaca aatttaacaa agaaatgtgg gcggcgtggg aagaaattcg 721 taacctgccg aacctgaacg gctggcagat gaccgcgttt attgcgagcc tggtggatga 781 tccgagccag agcgcgaacc tgctggcgga agcgaaaaaa ctgaacgatg cgcaggcgcc 841 gaaaaccggc ggtggttctg gtggtggttc tggtaccggc gctccgggaa aaaagaggcc 901 ggtagagcac tctcctgtgg agccagactc ctcctcggga accggaaagg cgggccagca 961 gcctgcaaga aaaagattga attttggtca gactggagac gcagactcag tacctgaccc 1021 ccagcctctc ggacagccac cagcagcccc ctctggtctg ggaactaata cgatggctac 1081 aggcagtggc gcaccaatgg cagacaataa cgagggcgcc gacggagtgg gtaattcctc 1141 gggaaattgg cattgcgatt ccacatggat gggcgacaga gtcatcacca ccagcacccg 1201 aacctgggcc ctgcccacct acaacaacca cctctacaaa caaatttcca gccaatcagg 1261 agcctcgaac gacaatcact actttggcta cagcacccct tgggggtatt ttgacttcaa 1321 cagattccac tgccactttt caccacgtga ctggcaaaga ctcatcaaca acaactgggg 1381 attccgaccc aagagactca acttcaagct ctttaacatt caagtcaaag aggtcacgca 1441 gaatgacggt acgacgacga ttgccaataa ccttaccagc acggttcagg tgtttactga 1501 ctcggagtac cagctcccgt acgtcctcgg ctcggcgcat caaggatgcc tcccgccgtt 1561 cccagcagac gtcttcatgg tgccacagta tggatacctc accctgaaca acgggagtca 1621 ggcagtagga cgctcttcat tttactgcct ggagtacttt ccttctcaga tgctgcgtac 1681 cggaaacaac tttaccttca gctacacttt tgaggacgtt cctttccaca gcagctacgc 1741 tcacagccag agtctggacc gtctcatgaa tcctctcatc gaccaatatt tgtattactt 1801 gagcagaaca aacactccaa gtggtaccac cacgcagagt cgacttcagt tttctcaggc 1861 cggagcgagt gacattcggg accagtctag gaactggctt cctggaccct gttaccgcca 1921 gcagcgagta tcaaagacat ctgcggataa caacaacagt gaatactcgt ggactggagc 1981 taccaagtac cacctcaatg gcagagactc tctggtgaat ccgggcccgg ccatggcaag 2041 ccacaaggac gatgaagaaa agttttttcc tcagagcggg gttctcatct ttgggaagca 2101 aggctcagag aaaacaaatg tggacattga aaaggtcatg attacagacg aagaggaaat 2161 caggacaacc aatcccgtgg ctacggagca gtatggatcc gtatctacca acctccaggc 2221 tggcaacacc ggcggcctga acgatatttt tgaagcgcag aaaattgaat ggcatgaagg 2281 cctgagcgcc caagcagcta cagctgatgt caacacacaa ggcgttcttc caggcatggt 2341 ctggcaggac agagatgtgt accttcaggg gcccatctgg gcaaagattc cacacacgga 2401 cggacatttt cacccctctc ccctcatggg tggattcgga cttaaacacc ctcctccaca 2461 gattctcatc aagaacaccc cagtacctgc gaatccttcg accaccttca gtgcggcaaa 2521 gtttgcttcc ttcatcacac agtactccac gggacaggtc agcgtggaga tcgagtggga 2581 gctccagaag gaaaacagca aacgctggaa tcccgaaatt cagtacactt ccaactacaa 2641 caagtctgtt aatgtggact ttactgtgga cactaatggc gtgtattcag agcctcgccc 2701 cattggcacc agatacctga ctcgtaatct gtaattgctt gttaatcaat aaaccgttta 2761 attcgtttca gttgaacttt ggtctctgcg tatttctttc ttatctagtt tccatggcta 2821 cgtagataag tagcatggcg ggttaatcat taactac //