LOCUS       BBa_K404163             2830 bp    DNA     linear       01-Jun-2024
DEFINITION  pCMV_Z-EGFR-1907_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-His-Ta
            g)
ACCESSION   BBa_K404163
VERSION     BBa_K404163.1  
FEATURES             Location/Qualifiers
     promoter        2..655
                     /label=CMV
     misc_feature    656..670
                     /label=scar
     CDS             671..844
                     /label=ZEGFR_1907
     misc_feature    845..850
                     /label=scar
     misc_feature    851..874
                     /label=middle-linker
     misc_feature    875..880
                     /label=scar
     CDS             881..2707
                     /label=VP2
     CDS             881..2830
                     /label=AAV-2-cap (Stratagene)
     misc_feature    884..904
                     /label=basic region 2
     misc_feature    962..982
                     /label=basic region 3
     CDS             1073..2707
                     /label=VP3
     misc_feature    1277..1306
                     /label=A20-1 binding site
     misc_feature    1385..1402
                     /label=basic region 4
     misc_feature    1574..1600
                     /label=A20-2 binding site
     misc_feature    2162..2191
                     /label=A20-4 binding site
     misc_feature    2196..2227
                     /label=upstream 587
     misc_feature    2219..2221
                     /label=HSPG-KO
     misc_feature    2228..2233
                     /label=TG-spacer
     misc_feature    2234..2251
                     /label=His-tag
     misc_feature    2252..2260
                     /label=GLS-spacer
     misc_feature    2261..2276
                     /label=downstream 587
     misc_feature    2288..2290
                     /label=HSPG-KO
     misc_feature    2675..2698
                     /label=B1 binding site
ORIGIN
        1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat
       61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg
      121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt
      181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac
      241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg
      301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact
      361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt
      421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc
      481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc
      541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata
      601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta
      661 gatggccggc gtggataaca aatttaacaa agaaatgtgg gcggcgtggg aagaaattcg
      721 taacctgccg aacctgaacg gctggcagat gaccgcgttt attgcgagcc tggtggatga
      781 tccgagccag agcgcgaacc tgctggcgga agcgaaaaaa ctgaacgatg cgcaggcgcc
      841 gaaaaccggc ggtggttctg gtggtggttc tggtaccggc gctccgggaa aaaagaggcc
      901 ggtagagcac tctcctgtgg agccagactc ctcctcggga accggaaagg cgggccagca
      961 gcctgcaaga aaaagattga attttggtca gactggagac gcagactcag tacctgaccc
     1021 ccagcctctc ggacagccac cagcagcccc ctctggtctg ggaactaata cgatggctac
     1081 aggcagtggc gcaccaatgg cagacaataa cgagggcgcc gacggagtgg gtaattcctc
     1141 gggaaattgg cattgcgatt ccacatggat gggcgacaga gtcatcacca ccagcacccg
     1201 aacctgggcc ctgcccacct acaacaacca cctctacaaa caaatttcca gccaatcagg
     1261 agcctcgaac gacaatcact actttggcta cagcacccct tgggggtatt ttgacttcaa
     1321 cagattccac tgccactttt caccacgtga ctggcaaaga ctcatcaaca acaactgggg
     1381 attccgaccc aagagactca acttcaagct ctttaacatt caagtcaaag aggtcacgca
     1441 gaatgacggt acgacgacga ttgccaataa ccttaccagc acggttcagg tgtttactga
     1501 ctcggagtac cagctcccgt acgtcctcgg ctcggcgcat caaggatgcc tcccgccgtt
     1561 cccagcagac gtcttcatgg tgccacagta tggatacctc accctgaaca acgggagtca
     1621 ggcagtagga cgctcttcat tttactgcct ggagtacttt ccttctcaga tgctgcgtac
     1681 cggaaacaac tttaccttca gctacacttt tgaggacgtt cctttccaca gcagctacgc
     1741 tcacagccag agtctggacc gtctcatgaa tcctctcatc gaccaatatt tgtattactt
     1801 gagcagaaca aacactccaa gtggtaccac cacgcagagt cgacttcagt tttctcaggc
     1861 cggagcgagt gacattcggg accagtctag gaactggctt cctggaccct gttaccgcca
     1921 gcagcgagta tcaaagacat ctgcggataa caacaacagt gaatactcgt ggactggagc
     1981 taccaagtac cacctcaatg gcagagactc tctggtgaat ccgggcccgg ccatggcaag
     2041 ccacaaggac gatgaagaaa agttttttcc tcagagcggg gttctcatct ttgggaagca
     2101 aggctcagag aaaacaaatg tggacattga aaaggtcatg attacagacg aagaggaaat
     2161 caggacaacc aatcccgtgg ctacggagca gtatggatcc gtatctacca acctccaggc
     2221 tggcaacacc ggccatcatc atcatcatca tggcctgagc gcccaagcag ctacagctga
     2281 tgtcaacaca caaggcgttc ttccaggcat ggtctggcag gacagagatg tgtaccttca
     2341 ggggcccatc tgggcaaaga ttccacacac ggacggacat tttcacccct ctcccctcat
     2401 gggtggattc ggacttaaac accctcctcc acagattctc atcaagaaca ccccagtacc
     2461 tgcgaatcct tcgaccacct tcagtgcggc aaagtttgct tccttcatca cacagtactc
     2521 cacgggacag gtcagcgtgg agatcgagtg ggagctccag aaggaaaaca gcaaacgctg
     2581 gaatcccgaa attcagtaca cttccaacta caacaagtct gttaatgtgg actttactgt
     2641 ggacactaat ggcgtgtatt cagagcctcg ccccattggc accagatacc tgactcgtaa
     2701 tctgtaattg cttgttaatc aataaaccgt ttaattcgtt tcagttgaac tttggtctct
     2761 gcgtatttct ttcttatcta gtttccatgg ctacgtagat aagtagcatg gcgggttaat
     2821 cattaactac
//