LOCUS BBa_K404164 3211 bp DNA linear 17-Dec-2024 DEFINITION pCMV_VP1up_NLS_Z-EGFR-1907_[AAV2]-VP23 (ViralBrick-587KO-Empty) ACCESSION BBa_K404164 VERSION BBa_K404164.1 FEATURES Location/Qualifiers promoter 2..655 /label=CMV misc_feature 673..1081 /label=VP1up misc_feature 1115..1228 /label=ZEGFR_1907 misc_feature 1295..3088 /label=VP2 misc_feature 1487..3088 /label=VP3 misc_feature 1088..1108 /label=NLS misc_feature 1295..3211 /label=AAV-2-cap (Stratagene) misc_feature 2642..2644 /label=HSPG-KO misc_feature 2633..2635 /label=HSPG-KO ORIGIN 1 gcgatgtacg ggccagatat acgcgttgac attgattatt gcctagttat taatagtaat 61 caattacggg gtcattagtt catagcccat atatggagtt ccgcgttaca taacttacgg 121 taaatggccc gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt 181 atgttcccat agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac 241 ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg 301 acgtcaatga cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact 361 ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt 421 ggcagtacat caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc 481 ccattgacgt caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc 541 gtaacaactc cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata 601 taagcagagc tctctggcta actagagaac ccactgctta ctggcttatc gaaattacta 661 gatggccggc gctgccgatg gttatcttcc agattggctc gaggacactc tctctgaagg 721 aataagacag tggtggaagc tcaaacctgg cccaccacca ccaaagcccg cagagcggca 781 taaggacgac agcaggggtc ttgtgcttcc tgggtacaag tacctcggac ccttcaacgg 841 actcgacaag ggagagccgg tcaacgaggc agacgccgcg gccctcgagc acgacaaagc 901 ctacgaccgg cagctcgaca gcggagacaa cccgtacctc aagtacaacc acgccgacgc 961 ggagtttcag gagcgcctta aagaagatac gtcttttggg ggcaacctcg gacgagcagt 1021 cttccaggcg aaaaagaggg ttcttgaacc tctgggcctg gttgaggaac ctgttaaggc 1081 gaccggccct gcaagaaaaa gattgaatac cggcgtggat aacaaattta acaaagaaat 1141 gtgggcggcg tgggaagaaa ttcgtaacct gccgaacctg aacggctggc agatgaccgc 1201 gtttattgcg agcctggtgg atgatccgag ccagagcgcg aacctgctgg cggaagcgaa 1261 aaaactgaac gatgcgcagg cgccgaaaac cggcgctccg ggaaaaaaga ggccggtaga 1321 gcactctcct gtggagccag actcctcctc gggaaccgga aaggcgggcc agcagcctgc 1381 aagaaaaaga ttgaattttg gtcagactgg agacgcagac tcagtacctg acccccagcc 1441 tctcggacag ccaccagcag ccccctctgg tctgggaact aatacgatgg ctacaggcag 1501 tggcgcacca atggcagaca ataacgaggg cgccgacgga gtgggtaatt cctcgggaaa 1561 ttggcattgc gattccacat ggatgggcga cagagtcatc accaccagca cccgaacctg 1621 ggccctgccc acctacaaca accacctcta caaacaaatt tccagccaat caggagcctc 1681 gaacgacaat cactactttg gctacagcac cccttggggg tattttgact tcaacagatt 1741 ccactgccac ttttcaccac gtgactggca aagactcatc aacaacaact ggggattccg 1801 acccaagaga ctcaacttca agctctttaa cattcaagtc aaagaggtca cgcagaatga 1861 cggtacgacg acgattgcca ataaccttac cagcacggtt caggtgttta ctgactcgga 1921 gtaccagctc ccgtacgtcc tcggctcggc gcatcaagga tgcctcccgc cgttcccagc 1981 agacgtcttc atggtgccac agtatggata cctcaccctg aacaacggga gtcaggcagt 2041 aggacgctct tcattttact gcctggagta ctttccttct cagatgctgc gtaccggaaa 2101 caactttacc ttcagctaca cttttgagga cgttcctttc cacagcagct acgctcacag 2161 ccagagtctg gaccgtctca tgaatcctct catcgaccaa tatttgtatt acttgagcag 2221 aacaaacact ccaagtggta ccaccacgca gagtcgactt cagttttctc aggccggagc 2281 gagtgacatt cgggaccagt ctaggaactg gcttcctgga ccctgttacc gccagcagcg 2341 agtatcaaag acatctgcgg ataacaacaa cagtgaatac tcgtggactg gagctaccaa 2401 gtaccacctc aatggcagag actctctggt gaatccgggc ccggccatgg caagccacaa 2461 ggacgatgaa gaaaagtttt ttcctcagag cggggttctc atctttggga agcaaggctc 2521 agagaaaaca aatgtggaca ttgaaaaggt catgattaca gacgaagagg aaatcaggac 2581 aaccaatccc gtggctacgg agcagtatgg atccgtatct accaacctcc aggctggcaa 2641 cgcccaagca gctacagctg atgtcaacac acaaggcgtt cttccaggca tggtctggca 2701 ggacagagat gtgtaccttc aggggcccat ctgggcaaag attccacaca cggacggaca 2761 ttttcacccc tctcccctca tgggtggatt cggacttaaa caccctcctc cacagattct 2821 catcaagaac accccggtac ctgcgaatcc ttcgaccacc ttcagtgcgg caaagtttgc 2881 ttccttcatc acacagtact ccacgggaca ggtcagcgtg gagatcgagt gggagctcca 2941 gaaggaaaac agcaaacgct ggaatcccga aattcagtac acttccaact acaacaagtc 3001 tgttaatgtg gactttactg tggacactaa tggcgtgtat tcagagcctc gccccattgg 3061 caccagatac ctgactcgta atctgtaatt gcttgttaat caataaaccg tttaattcgt 3121 ttcagttgaa ctttggtctc tgcgtatttc tttcttatct agtttccatg gctacgtaga 3181 taagtagcat ggcgggttaa tcattaacta c //